اندازه‌گیری محتوای برخی متابولیت‌های ثانویه زیست فعال و برخی فلانونوئیدهای گیاه سلمکی ‏‏(‏Atriplex patula L.‎‏) در مراحل مختلف فنولوژیکی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشیار، دانشکده کشاورزی، دانشگاه بوعلی سینا، همدان، ایران

2 دانش‌آموخته کارشناسی ارشد، دانشکده کشاورزی، دانشگاه بوعلی سینا، همدان، ایران

3 استادیار، دانشکده کشاورزی، دانشگاه بوعلی سینا، همدان، ایران

چکیده

در این آزمایش تأثیر مراحل فنولوژیکی گیاه بر برخی ترکیبات زیست فعال و فلاونوئیدهای روتین و کوئرستین گیاه سلمکی (Atriplex patula L.) مورد بررسی قرار گرفت. سه مرحله فنولوژیکی رویشی (هشت برگی)، گل­دهی و بذردهی به عنوان سه تیمار آزمایش لحاظ گردید. در سه مرحله فنولوژیکی برخی از ترکیبات بیوشیمایی و کوئرستین و روتین گیاه اندازه گیری شدند. نتایج نشان داد بیش­ترین و کمترین (06/2 و 88/0 میلی گرم در گرم وزن تر) میزان کلروفیل کل به ترتیب در مرحله بذر­دهی و مرحله رویشی مشاهده شد. بیشترین و کمترین میزان کلروفیل a، کلروفیل b و کاروتنوئید به ترتیب در مرحله بذر دهی و مرحله رویشی یافت شد. بیشترین مقدار فنل کل (76/132 میلی گرم اسید گالیک در 100 گرم وزن تر)، تانن کل (057/0 میلی گرم اسید تانیک در 100 گرم وزن تر) و ظرفیت آنتی­اکسیدانی (28/50 درصد) در مرحله گل­دهی و کم­ترین مقدار فنل کل (24/92 میلی گرم اسید گالیک در 100 گرم وزن تر)، تانن کل (041/0 میلی گرم اسید تانیک در 100 گرم وزن تر) و فعالیت آنتی اکسیدانی (95/16 درصد) در مرحله رویشی مشاهده شد. بیش­ترین و کمترین میزان فلاونوئید کل (05/88 و 89/35 میلی گرم روتین در 100 گرم وزن تازه) به ترتیب در مرحله بذر­دهی و رویشی مشاهده شد. بیش­ترین و کم­ترین مقدار کوئرستین به ترتیب در مرحله بذر­دهی و رویشی (05/0 و 01/0 میلی‌گرم در گرم وزن خشک) مشاهده شد. بیشترین و کمترین مقدار روتین (11/2 و 85/1 میلی گرم در گرم وزن خشک) در مرحله بذر­دهی و مرحله رویشی یافت گردید. با توجه به میزان بالای برخی از ترکیبات بیوشیمیایی در گیاه سلمکی می‌توان در آینده از پتاسیل دارویی این گیاه بهره برد.

کلیدواژه‌ها

عنوان مقاله [English]

Evaluating the content of some bioactive secondary metabolites and flavonoids in ‎Atriplex patula at different phenological stages

نویسندگان [English]

  • Mohammad Sayyari 1
  • Sameyea Ghaeloni 2
  • Ali Azizi 3
1 Associate Professor, Faculty of Agriculture, BuAli Sina University, Hamedan, ‎Iran
2 Graduated M. Sc, Faculty of Agriculture, BuAli Sina University, Hamedan, Iran
3 Assistant Professor, Faculty of Agriculture, BuAli Sina University, Hamedan, Iran
چکیده [English]

This experiment was performed to determine the content of some bioactive compounds and flavonoids of Atriplex patula L. at different phenological stages. Three phonological stages, including vegetative (eight-leaf), flowering and seed production stages, were considered as three treatments of experiment. Some biochemical parameters and also rutin and quercetin were measured at different growth stages. The results showed that the highest (2.06 mg g-1) and lowest (0.88 mg g-1) total chlorophyll content was observed at seed production and vegetative stages, respectively. Also, the highest chlorophyll ‘a’, chlorophyll ‘b’ and carotenoids contents were observed in the seed production and vegetative stages, respectively. The highest amount of total phenols (132.76 mg gallic acid 100g-1 FW), total tannin (0.057 mg tannic acid 100g-1 FW) and antioxidant capacity (50.28%) were found at flowering stage, while the lowest amount of total phenols (92.24 mg gallic acid 100g-1 FW), total tannin (0.041 mg tannic acid 100g-1 FW) and antioxidant capacity (16.95%) were observed at vegetative stage. The highest and lowest (88.05 and 35.95 mg rutin 100g-1 FW) amount of total flavonoid was found at seed production and vegetative stages. The highest and lowest quercetin levels were observed in seed production and vegetative stages (0.05 and 0.01 mg g-1 dry weight), respectively. Also, the highest and lowest (2.11 and 1.85 mg g-1 dry weight) amount of rutin were found in the seed production and vegetative stages. Due to the high amount of some biochemical compounds in Atriplex patula, the medicinal potential of this plant can be used in the future.

کلیدواژه‌ها [English]

  • Antioxidant capacity
  • quercetin
  • rutin
  • tannin
  • total phenolics

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علوم باغبانی ایران
دوره 53، شماره 4
دی 1401
صفحه 859-868

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سابقه مقاله

  • تاریخ دریافت: 07 بهمن 1399
  • تاریخ بازنگری: 21 اسفند 1400
  • تاریخ پذیرش: 24 فروردین 1401

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