اثر سدیم نیترو پروساید بر برخی ویژگی‌های بیوشیمیایی و پاداکسندگی میوه ازگیل ‏‏(‏Mespilus germanica L.‎‏) طی انبارمانی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانش‌آموخته کارشناسی ارشد، دانشکده کشاورزی، دانشگاه ارومیه، ارومیه، ایران

2 دانشیار، دانشکده کشاورزی، دانشگاه ارومیه، ارومیه، ایران

چکیده

از رویشگاه­های طبیعی ازگیل در ایران، استان آذربایجان شرقی منطقه ارسباران می­باشد. به منظور بررسی تأثیر سدیم نیترو پروساید در 4 غلظت صفر، 3، 5 و 8 میکرومولار بر صفات کمی و کیفی میوه ازگیل طی 5 زمان (صفر، 15، 30، 45 و 60 روز) نگهداری در انبار سرد با دمای 1±1 درجه سانتی­گراد و رطوبت نسبی 95-85 درصد، پژوهشی به‌صورت فاکتوریل در قالب طرح کاملاً تصادفی با 3 تکرار انجام شد. بر اساس نتایج بیشترین و کمترین درصد کاهش وزن میوه در طول دوره انبارداری به ترتیب در شاهد (24/2 درصد) و 5 میکرومولار (36/1درصد) به‌دست آمد. شاخص‌های مرتبط با رنگ میوه شامل L*, Hue, Chroma و میزان سفتی میوه در غلظت 5 میکرومولار سدیم نیترو پروساید نسبت به شاهد بهتر حفظ شدند. سدیم نیترو پروساید در غلظت 3 میکرومولار توانست میزان فنل کل، فلاونوئید کل، تانن کل را در حد بالایی حفظ کند و از افزایش فعالیت آنزیم پلی فنل اکسیداز(PPO) در طول دوره انبارداری نسبت به شاهد جلوگیری کند. همچنین میزان ظرفیت آنتی‌اکسیدانی کل به روش دی پی پی اچ (DPPH) و فعالیت آنزیم‌های آنتی‌اکسیدانی کاتالاز (CAT) و گایاگول پراکسیداز (GPX) در غلظت 8 میکرومولار سدیم نیترو پروساید نسبت به شاهد بیشتر بود. بنابراین به نظر می‌رسد سدیم نیترو پروساید با حفظ تمامیت غشاء، حفظ ترکیبات فنلی و کاهش فعالیت آنزیم PPO باعث افزایش کیفیت پس از برداشت میوه ازگیل شده است. بطور کلی می­توان گفت که غلظت 3 میکرومولار سدیم نیترو پروساید در طی انباداری توانست از کاهش بیش از حد صفات مرتبط با کیفیت میوه ازگیل جلوگیری کند. 

کلیدواژه‌ها


عنوان مقاله [English]

Effect of sodium nitroprusside on biochemical and antioxidant properties of medlar ‎‎(Mespilus germanica L.) fruit during storage period

نویسندگان [English]

  • Bashir Ayob nezhadghan 1
  • Hamid Hassanpour 2
1 Graduate M.Sc., Faculty of Agriculture, Urmia University, Urmia, Iran
2 Associate Professor, Faculty of Agriculture, Urmia University, Urmia, Iran
چکیده [English]

Medlar (Mespilus germanica L.) is grown wildly in the East Azerbaijan province (Arasbaran). To evaluate the effect of different concentrations of sodium nitroprusside (SNP)(0, 3, 5 and 8 µM /L) on quantitative and qualitative parameters of medlar fruits during cold storage (1±1°C and 85-95% relative humidity) at five storage times (0, 15, 30, 45 and 60 days), a study was conducted in a factorial experiment based on a completely randomized design with three  replications. According to the results, the highest and lowest percentage of fruit weight loss were obtained at control (2.24 %) and 5 μM/L SNP (1.36 %) during storage, respectively. Fruit color parameters such as L*, hue, chroma and firmness of fruit were maintained at 5 μM/L SNP compared to control. The SNP at 3 µM/L was able to maintain total phenol, total flavonoid, total tannin at high levels and prevent increased polyphenol oxidase (PPO) activity during storage compared to the control. Also, total antioxidant capacity based on DPPH method and activity of antioxidant enzymes such as catalase (CAT) and guaiacol peroxidase (GPX) at 8 µM/L SNP were higher than control. Therefore, SNP seems to increase the postharvest quality of medlar fruit via maintaining the membrane integrity, maintaining phenolic compounds, and reducing the activity of PPO enzyme. In general, it can be concluded that the SNP at 3 µM/L could prevent the excessive reduction of fruit compounds during the storage period.

کلیدواژه‌ها [English]

  • Antioxidant Enzymes
  • fruit color
  • phenolic compounds
  • tannin‎
  1. Abd Elahi, R., Asghari, M.R. & Esmailli, M. (2010). Effect of nitric oxide and putrescine on quality properties and postharvest life of strawberry fruits (cv. Selva). Journal of Food Industry Research, 20(1), 177-190. (In Farsi).
  2. Aebi, H. (1984). Catalase in vitro. Methods in Enzymology, 105, 121-126.
  3. Asghari, M.R., Ghafari Baktash, H., Rasmi, Y. & Farokhzad, A.R. (2014). Study of the interaction of salicylic acid and nitric oxide after harvesting in preserving the quality and increasing the marketability of apple fruit (cv. Red delicious). Journal of Production and Processing of Crop and Gardening, 4(13), 51-61. (In Farsi).
  4. Asghari, M.R. & Khomeyri Sani, M. (2010). Effect of postharvest putrescine and nitric oxide application on some quality attributes and total content phenolics on table grape (cv. Sefide bidane). Journal of Food Industry Research, 3(2), 61-72. (In Farsi).
  5. Atares, L., Perez Masia, R. & Chiralt, A. (2011). The role of some antioxidants in the HPMC film properties and lipid protection in coated toasted almonds. Journal of Food Engineering, 104(4), 649-659.
  6. Bahamir, S. & Mohammad Khani, A. (2014). Effect of nitric oxide on qualitative properties and after harvesting of strawberry fruit (cv. Gaviyata). First Iranian National Technology Congress, 1-9. (In Farsi).
  7. Broadhurst, R.B. & Jones, W.T. (1978). Analysis of condensed tannins using acidified vanillin. Journal of the Science of Food and Agriculture, 29, 788-794.
  8. Du, G., Li, M., Ma, F. & Liang, D. (2009). Antioxidant capacity and the relationship with polyphenol and vitamin C in Actinidia Food Chemistry, 113, 557-562.
  9. Erkan, M. & Selcuk, N. (2015). The effects of 1-MCP treatment on fruit quality of medlar fruit (Mespilus germanica cv. Istanbul) during long term storage in the palliflex storage system. Postharvest Biology and Technology, 100, 81–90.
  10. Flores, F.B., Sanchez-Bel, P., Valdengro, M., Romojaro Maria, F., Martinez-Madrid, C. & Isabel Egea, M. (2008). Effects of a pretreatment with nitric oxide on peach (Prunus persica) storage at room temperature. European Food Research and Technology, 227, 1599-1611.
  11. Ghorbani, B. & PakKish, Z. (2017). Effect of nitric oxide on reducing the adverse effects of cold on orange fruit (Citrus sinensis cv. Washington Navel) during storage. Journal of Horticulture, 31(3), 492-504. (In Farsi).
  12. Gulcin, I., Topal, F., Sarikaya, S.B., Bursal, E., Bilsel, G. & Goren, A.C. (2011). Polyphenol contents and antioxidant properties of medlar (Mespilus germanica ). Records of Natural Products, 5(3), 158-175.
  13. Hedayati, G.H. & Sadeghi, F. (2021). Effects of sodium nitroprusside (SNP) on marketability and qualitative properties of Mexican lime fruit (Citrus aurantifolia) at storage. Iranian Journal of Horticultural Science, 51(4), 913-923. (In Farsi).
  14. Huang, K.T. & Kao, C.H. (2005). Nitric oxide counteracts the senescence of rice leaves induced by hydrogen peroxide. Botanical Bulletin of Academia Sinica, 46, 21-28.
  15. kang, H.M. & Saltveit, M.E. (2002). Chilling tolerance of maize, cucumber and rice seedling leaves and roots and differentially affected by salicylic acid. Plant Physiology, 115, 571-576.
  16. Khoshbakht, K. & Hammer, K. (2005). Notes on neglected and underutilized crops. Genetic Resources and Crop Evolution, 52, 249-265.
  17. Lineh, T.T.T., Jitareerat, P., Aimla-or, S., Srilaong, V., Boonyaritthongchai, P. & Uthairatanakij, A. (2015). Applying of sodium nitroprusside (SNP) on postharvest mango fruits delay ripening and maintain quality. African Journal of Agricultural Research, 10(31), 3067-3072
  18. Meng, X., Libliu, J. & Tian, S. (2007). Physiological responses and quality attributes of table grape fruit to chitosan perharvest spray and postharvest coating during storage. Food Chemistry, 106, 501-508.
  19. Moradinezhad, F., Ranjbari, F. & Khayyat, M. (2016). Effect of nitric oxide on biochemical and antioxidant properties of pomegranate fruit cv. Shishe-kab during cold storage. International Journal of Horticultural Science and Technology, 3(2), 211-219.
  20. Mostofi, Y., Rasouli, P., Naderi, R., Bagheri Marandi, G. & Shafiei, M.R. (2011). Effect of nitric oxide and thidiazuron on vase life and some qualitative characteristics of cut carnation flowers (Dianthus caryophyllus Nelson). Iranian Journal of Horticultural Science, 41(4), 301-308. (In Farsi).
  21. Mouzafariyan, V. (2004). Iran trees and Shrubs. Tehran Contemporary Culture, 1003 pages. (In Farsi).
  22. Nakajima, J.I., Tanaka, I., Seo, S., Yamazaki, M. & Saito, K. (2004). LC/PDA/ESI- MS profiling and radical scavenging activity of anthocyanins in various berries. Journal of Biomedicine and Biotechnology, 5, 241–247.
  23. Nasibi, F. (2011). Effect of different sodium nitroproside concentrations on reduction of oxidative damage caused by drought stress in Tomato plant. Journal of Plant Biology, 3(9), 63-74. (In Farsi).
  24. Parvaneh, V. (1992). Food Quality Control and Chemistry Analysis. University of Tehran Publication, 322. (In Farsi).
  25. Pizzocaro, F., Torreggiani, & Gilardi, G. (1993). Inhibition of apple polyphenoloxidase (PPO) by ascorbicacid, citric acid and sodium chloride. Journal of Food Processing and Preservation, 17, 21-30.
  26. Shin, Y., Liu, R.H., Nock, J.F., Holliday, D. & Watkins, C.B. (2007). Temperature and relative humidity effects on quality, total ascorbic acid, phenolics and flavonoid concentrations, and antioxidant activity of strawberry. Postharvest Biology and Technology, 45, 349-357.
  27. Shuhua, Z., Lina, S., Mengchen, L. & Jie, Z. (2008). Effect of nitric oxide on reactive oxygen species and antioxidant enzymes in kiwifruit during storage. Journal of the Science of Food and Agriculture, 88, 2324-2331.
  28. Singh, S.P., Singh, Z. & Swinny, E.E. (2009). Postharvest nitric oxide fumigation delays fruit ripening and alleviates chilling injury during cold storage of Japanese plums (Prunus salicina Lindell). Postharvest Biology and Technology, 53, 101-108.
  29. Updhyaya, A., Sankhla, D., Davis, T.D., Sankhla, N. & Smidth, B.N. (1985). Effect of paclobotrazol on the activities of some enzymes of activated oxygen metabolism and lipid peroxidation in senescing soybean leaves. Journal of Plant Physiology, 121, 453-461.
  30. Xueqing, L., Jae kyung, K., Yunsheng, L. & Fang Liu, X.Ch. (2005). Tannic acid stimulates Glucose Transport and Inhibits Adipocyte differentiation in 3T3-L1 cells. Journal of Nutrition, 135(2), 165-171.
  31. Zheng, Y., Wang, C.Y., Wang, S.Y. & Zheng, W. (2003). Effect of high-oxygen atmospheres on blueberry phenolic, anthocyanins, and antioxidant capacity. Journal of Agricultural and Food Chemistry, 51, 7162-7169.
  32. Zhu, S., Sun, L. & Zhou, J. (2009). Effects of nitric oxide fumigation on phenolic metabolism of postharvest chinese winter jujube ( Zizyphus jujube Mill. cv. Dongzao) in relation to fruit quality. Food Science and Technology, 42, 1009-1014.
  33. Zhua, S., Liu, M. & Zhou, J. (2006). Inhibition by nitric oxide of ethylene biosynthesis and lipoxygenase activity in peach fruit during storage. Postharvest Biology and Technology, 42, 41-48.