اثر سدیم نیترو پروساید بر برخی ویژگی‌های بیوشیمیایی و پاداکسندگی میوه ازگیل ‏‏(‏Mespilus germanica L.‎‏) طی انبارمانی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانش‌آموخته کارشناسی ارشد، دانشکده کشاورزی، دانشگاه ارومیه، ارومیه، ایران

2 دانشیار، دانشکده کشاورزی، دانشگاه ارومیه، ارومیه، ایران

چکیده

از رویشگاه­های طبیعی ازگیل در ایران، استان آذربایجان شرقی منطقه ارسباران می­باشد. به منظور بررسی تأثیر سدیم نیترو پروساید در 4 غلظت صفر، 3، 5 و 8 میکرومولار بر صفات کمی و کیفی میوه ازگیل طی 5 زمان (صفر، 15، 30، 45 و 60 روز) نگهداری در انبار سرد با دمای 1±1 درجه سانتی­گراد و رطوبت نسبی 95-85 درصد، پژوهشی به‌صورت فاکتوریل در قالب طرح کاملاً تصادفی با 3 تکرار انجام شد. بر اساس نتایج بیشترین و کمترین درصد کاهش وزن میوه در طول دوره انبارداری به ترتیب در شاهد (24/2 درصد) و 5 میکرومولار (36/1درصد) به‌دست آمد. شاخص‌های مرتبط با رنگ میوه شامل L*, Hue, Chroma و میزان سفتی میوه در غلظت 5 میکرومولار سدیم نیترو پروساید نسبت به شاهد بهتر حفظ شدند. سدیم نیترو پروساید در غلظت 3 میکرومولار توانست میزان فنل کل، فلاونوئید کل، تانن کل را در حد بالایی حفظ کند و از افزایش فعالیت آنزیم پلی فنل اکسیداز(PPO) در طول دوره انبارداری نسبت به شاهد جلوگیری کند. همچنین میزان ظرفیت آنتی‌اکسیدانی کل به روش دی پی پی اچ (DPPH) و فعالیت آنزیم‌های آنتی‌اکسیدانی کاتالاز (CAT) و گایاگول پراکسیداز (GPX) در غلظت 8 میکرومولار سدیم نیترو پروساید نسبت به شاهد بیشتر بود. بنابراین به نظر می‌رسد سدیم نیترو پروساید با حفظ تمامیت غشاء، حفظ ترکیبات فنلی و کاهش فعالیت آنزیم PPO باعث افزایش کیفیت پس از برداشت میوه ازگیل شده است. بطور کلی می­توان گفت که غلظت 3 میکرومولار سدیم نیترو پروساید در طی انباداری توانست از کاهش بیش از حد صفات مرتبط با کیفیت میوه ازگیل جلوگیری کند. 

کلیدواژه‌ها

عنوان مقاله [English]

Effect of sodium nitroprusside on biochemical and antioxidant properties of medlar ‎‎(Mespilus germanica L.) fruit during storage period

نویسندگان [English]

  • Bashir Ayob nezhadghan 1
  • Hamid Hassanpour 2
1 Graduate M.Sc., Faculty of Agriculture, Urmia University, Urmia, Iran
2 Associate Professor, Faculty of Agriculture, Urmia University, Urmia, Iran
چکیده [English]

Medlar (Mespilus germanica L.) is grown wildly in the East Azerbaijan province (Arasbaran). To evaluate the effect of different concentrations of sodium nitroprusside (SNP)(0, 3, 5 and 8 µM /L) on quantitative and qualitative parameters of medlar fruits during cold storage (1±1°C and 85-95% relative humidity) at five storage times (0, 15, 30, 45 and 60 days), a study was conducted in a factorial experiment based on a completely randomized design with three  replications. According to the results, the highest and lowest percentage of fruit weight loss were obtained at control (2.24 %) and 5 μM/L SNP (1.36 %) during storage, respectively. Fruit color parameters such as L*, hue, chroma and firmness of fruit were maintained at 5 μM/L SNP compared to control. The SNP at 3 µM/L was able to maintain total phenol, total flavonoid, total tannin at high levels and prevent increased polyphenol oxidase (PPO) activity during storage compared to the control. Also, total antioxidant capacity based on DPPH method and activity of antioxidant enzymes such as catalase (CAT) and guaiacol peroxidase (GPX) at 8 µM/L SNP were higher than control. Therefore, SNP seems to increase the postharvest quality of medlar fruit via maintaining the membrane integrity, maintaining phenolic compounds, and reducing the activity of PPO enzyme. In general, it can be concluded that the SNP at 3 µM/L could prevent the excessive reduction of fruit compounds during the storage period.

کلیدواژه‌ها [English]

  • Antioxidant enzymes
  • fruit color
  • phenolic compounds
  • tannin‎

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علوم باغبانی ایران
دوره 52، شماره 1
خرداد 1400
صفحه 257-268

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سابقه مقاله

  • تاریخ دریافت: 10 شهریور 1398
  • تاریخ بازنگری: 08 آذر 1398
  • تاریخ پذیرش: 29 بهمن 1398

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