ارزیابی ترکیبات اسانس و اثر ضد‌باکتریایی پونه (‏Mentha longifolia‏) جمع‌آوری‌شده از مناطق ‏مختلف لرستان

نوع مقاله : مقاله پژوهشی

نویسندگان

1 استادیار، گروه کشاورزی، دانشگاه پیام نور، تهران، ایران

2 دانشجوی دکتری، مرکز تحقیقات داروهای گیاهی رازی، دانشگاه علوم پزشکی لرستان، خرم‌آباد، ‏ایران

3 استادیار، دانشکده کشاورزی، دانشگاه ولی عصر (عج) رفسنجان، رفسنجان، ایران

4 دانش‌آموخته کارشناسی ارشد، مرکز تحقیقات داروهای گیاهی رازی، دانشگاه علوم پزشکی لرستان، خرم‌آباد، ایران

چکیده

تحقیق حاضر با هدف بررسی ترکیبات شیمیایی اسانس استخراج شده از پونه جمع­آوری‌شده از مناطق مختلف شامل خرم‌آباد، الشتر و دلفان در استان لرستان و تعیین فعالیت ضد­باکتریایی آنها علیه سه سویه باکتری Escherichia coli، Staphylococcus aureus و Pseudomonas aeruginosa انجام شد. آنالیز GC-MS نشان داد ترکیبات اصلی اسانس پونه منطقه خرم‌آباد شامل پولگان (41/54 درصد) و 8،1- سینئول (05/22 درصد) بود، درحالی‌که پونه منطقه الشتر غنی از پیپریتنون (29/29 درصد)، پولگان (53/17 درصد)، پیپریتنون­اکساید (35/14 درصد) و 8،1- سینئول (34/14 درصد) و ترکیبات اصلی پونه منطقه دلفان سیترونلیل­استات (93/59 درصد) و آروماندرن (1/5 درصد) بودند. همبستگی معنی‌داری بین تمام ویژگی­های جغرافیایی و خاک، به‌جز فسفر و هدایت الکتریکی، با ترکیبات اسانس مشاهده شد. با توجه به  نتایج به‌دست‌آمده از MIC و MBC نشان داد حداقل غلظت بازدارندگی اسانس پونه مناطق مورد مطالعه بین mg/mL 2-03/0 بود. فعالیت ضدمیکروبی حاکی از این بود که مؤثرترین اسانس در مهارکنندگی باکتری­های E. coliو  S. aureus به‌ترتیب اسانس پونه منطقه الشتر و دلفان بودند و دو باکتری P. aeruginosa و S. aureus کمترین حساسیت به اسانس پونه منطقه خرم‌آباد داشتند. اسانس پونه جمع­آوری شده از مناطق مختلف استان لرستان دارای قدرت بازدارندگی علیه باکتری­های گرم مثبت و منفی مورد مطالعه از کم تا عالی بودندکه بستگی به منطقه جمع­آوری گیاهان و سویه­های مورد مطالعه باکتری داشت. در این تحقیق سه ترکیب سیترونلال، سیترونلیل­استات و آرومادرن برای اولین بار برای اسانس پونه گزارش شد.

کلیدواژه‌ها


عنوان مقاله [English]

Evaluation of essential oil composition and antibacterial effect of Mentha longifolia ‎collected from different region of Lorestan province

نویسندگان [English]

  • Soheila Afkar 1
  • Mozhgan Azadpour 2
  • Batool Mahdavi 3
  • Marzieh rashidipour 4
1 Assistant Professor, Department of Agriculture, Payame Noor University, Tehran, Iran
2 Ph.D. Candidate, Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, ‎Khorramabad, Iran
3 Assistant Professor, Faculty of Agriculture, Vali-e-Asr University of Rafsanjan, Rafsanjan, Iran
4 Former M.Sc. Student, Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran
چکیده [English]

The aim of this study was to investigate of the chemical composition of the essential oil extracted from Mentha longifolia collected from different regions of Lorestan province (Khorramabad, Aleshtar, Delfan) and to determine the antibacterial activities against three certain bacterial strains (Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli). The chemical profiling of the essential oil was performed using gas chromatography-mass spectrometry (GC-MS) analysis. GC-MS analysis revealed that essential oil of M. longifolia from Khorramabad region was constituted by pulegone (54.41%) and 1, 8-cineole (22.05%) as a major component. M. longifolia from Aleshtarwas rich in piperitenone (29.29%), pulegone (17.53%), piperitenone oxide (14.35%), 1, 8-cineole (14.34%) and the main component of M. longifolia from Delfanwas citronelyll acetate (59.93%), aromadrene (5.1%), respectively. A significant correlation between geographical and soil characteristics, except phosphorus and electrical conductivity, with the essential oils components was detected. The results obtained using MIC and MBC showed that the minimum inhibitory concentration of the essential oil of M. longifolia from studied regionwas in the range of 0.03-2 mg/mL. The most effective essential oil in bacterial inhibition for E. coli and S. aureus bacteria was the essential oil from Aleshtar and Delfan, respectively. Two bacteria P. aeruginosa and S. aureus were least susceptible to M. longifolia essential oil from Khorramabad region. The essential oil of M. longifolia from different regions of Lorestan provinc exhibited low to high inhibitory activity against gram-positive and negative bacteria,.which depended to the region of collected plants and studied bacterial strains. In this research, three components citronellyl acetat, citronellal and aromandrene was detected in M. longifolia essential oil for the first time

کلیدواژه‌ها [English]

  • Antibacterial activity
  • Chemical composition
  • Essential oil
  • Lorestan
  • Mentha longifolia‎
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