گزینش به‌کمک نشانگر ‏SNP‏ به‌منظور به‌نژادی صفت شیرینی میوه در طالبی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد، پردیس ابوریحان دانشگاه تهران، تهران، ایران

2 دانشیار، پردیس ابوریحان دانشگاه تهران، تهران، ایران

3 دانشجوی دکتری، پردیس ابوریحان دانشگاه تهران، تهران، ایران

چکیده

این تحقیق باهدف به­نژادی صفت شیرینی میوه در طالبی رقم ساوه انجام شد. برای این منظور تلاقی بین رقم ساوه با رقم گالیا انجام شد و نسل F2 تشکیل شد. برای اندازه­گیری شیرینی میوه طالبی در نسل والدین و نسل F2 از دستگاه رفراکتومتر استفاده شد. در نسل F2 42 گیاه از 2100 گیاه انتخاب و ارزیابی شد. همچنین سه نشانگر SNP مرتبط با شیرینی میوه در جمعیت F2با روش HRM با کمک دستگاه ریل تایم پی‌سی‌آر تعیین ژنوتیپ گردید. از بین سه نشانگر SNP یکی از آن­ها (SlE1)  بین والدین چندشکلی نشان داد و رقم گالیا و ساوه به­ترتیب دارای ژنوتیپ A1A1 و A2A2 بودند. پس از تعیین ژنوتیپ به‌ترتیب 13، 23 و 6 گیاه برای سه ژنوتیپ A1A1، A1A2 و A2A2  به‌دست آمد. تجزیه واریانس طرح کاملاً­ تصادفی نامتعادل نشان داد این سه گروه از نظر میزان قند، تفاوت معنی­داری داشتند. میانگین میزان قند در واحد بریکس (%) برای سه ژنوتیپ A1A1، A1A2 و A2A2 به­ترتیب 75/11، 85/10 و 68/6  به‌دست آمد. مقایسه میانگین با روش دانکن نشان داد تفاوت این میانگین‌ها در سطح احتمال 01/0 p< معنی­دار بود. این نتایج نشان می­دهد نشانگر SlE1-HRM به‌خوبی می­تواند گیاهانی را گزینش کند که میزان قند آن­ها نسبت به والد ساوه بیشتر است.   

کلیدواژه‌ها


عنوان مقاله [English]

SNP marker assisted selection for improving the sugar content in the cantaloupe

نویسندگان [English]

  • Fatemeh Ramezani 1
  • Hossein Ramshini 2
  • Mahmoud Lotfi 2
  • Mohammad Mahdi Mortazavian 2
  • Safdar Pour-Mombeini 3
1 M. Sc. Student, Abu Raihan Campus, Univesrity of Tehran, Tehran, Iran
2 Associate Professor, Abu Raihan Campus, Univesrity of Tehran, Tehran, Iran
3 Ph. D. Candidate, Abu Raihan Campus, Univesrity of Tehran, Tehran, Iran
چکیده [English]

This research was carried out with the aim of improving fruit sugar content in cantaloupe, accession Saveh. The ‘Saveh’ was crossed with ‘Galia’ and the F2 population was generated. The sugar content in fruits of parental generations and F2 population was measured using a refractometer. In F2 population 42 plants out of 2100 plants were selected and evaluated. HRM technique was used to determine the genotype of three candidate SNP markers which previously had been reported strongly associated with sugar content in fruit. The HRM analysis was carried out using Real Time PCR. Among the three SNP markers, one of them (SlE1-HRM) showed polymorphism. ‘Galia’ and ‘Saveh’ genotypes had A1A1 and A2A2 genotypes, respectively. After determining the genotypes, 13, 23 and 6 plants were obtained for three genotypes of A1A1, A1A2 and A2A2 , respectively. Unbalanced completely randomized design analysis of variance showed that these three groups had a significant difference in sugar content. The average of sugar content in brix unit (%) for A1A1, A1A2 and A2A2 genotypes was 11.75, 10.85 and 6.68, respectively. The mean comparison with Duncan's method showed that the difference of these average values was significant at the probability level of p <0.01. These results indicate that the marker can well select the plants that have higher sugar content than the parent of Saveh.

کلیدواژه‌ها [English]

  • Galia
  • HRM method
  • Realtime PCR
  • Saveh‎
  1. Aharoni, Y., Copel, A. & Fallik, E. (1993). Storing ‘Galia’ melons in a controlled atmosphere with ethylene absorbent. HortScience, 28(7), 725-726.
  2. Albuquerque, B., Lidon, F.C. & Graca Barreiro, M. (2006(. A case study on the flavor properties of melon (Cucumis melo L.) cultivars. Fruits, 61(5), 333-339.
  3. Bahrami Sirmandi, S., Bahrami Sirmandi, H. & Hasanzadeh, R. A. (2013). A complete and pictured guide of vegetable culture and breeding. Sarva Publishers, 244p. (in Farsi)
  4. Burger, Y., Saar, U., Katzir, N., Paris, H, S., Yeselson, Y., Levin, I. & Schaffer, A. (2002). A single recessive gene for sucrose accumulation in Cucumis melo fruit. Journal of American Society for Horticultural Science, 127(6), 938-943.
  5. Diaz, A., Fergany, M., Formisano, G., Ziarsolo, P., Blanca, J., Fei, Z., Staub, J.E., Zalapa, J.E., Cuevas, H.E., Dace, G. & Oliver, M. (2011). A consensus linkage map for molecular markers and Quantitative Trait Loci associated with economically important traits in melon (Cucumis melo L.). BMC Plant Biology, 11, 111-125.
  6. Diba Zar, Sh., Sheikhi, N., Hemmat Zadeh, F., Charkh Kar, S. & Pour Bakhsh, A. (2014). Use the HRM method to detect and subtract the Iranian isolate from Newcastle disease from vaccine strains. Journal of Camparative Pathobiology, 11(3), 1345-1356.
  7. Fallik, E., Alkali-tuvia, S., Horev, B., Copel, A., Rodov, V., Aharoni, Y., Ulrich, D. & Schulz, H. (2001). Characterisation of “Galia” melon aroma by GC and mass spectrometric sensor measurements after prolonged storage. Postharvest Biology and Technology, 22, 85-91.
  8. Food and Agricultural Organization. (2016). Biodiversity: Agricultural biodiversity in FAO. Retrieved October 12, 2018. From: http://faostat.fao.org/site/339/default.aspx.
  9. Gross, K.C., Wang, C.Y. & Salveit, M. (2016). The commercial storage of fruits, vegetables and florist and nursery stocks. United States Department of Agriculture.
  10. Han, Y., Khu, D-M. & Monteros, M. J. (2012). High-resolution melting analysis for SNP genotyping and mapping in tetraploid alfalfa (Medicago sativa L.). Molecular Breeding, 29, 489-501.
  11. Hwang, J.H., Ahn, S.G., Oh, J.Y., Choi, Y.W., Kang, J.S. & Park, Y.H. (2011). Functional characterization of watermelon (Citrullus lanatus L.) EST-SSR by gel electrophoresis and high resolution melting analysis. Scientia Horticulturea. (Amsterdam), 130, 715-724.
  12. Kaviani Charati, A., Sabouri, H., Fallahi, H.A. & Jorjani, E. (2016) QTL mapping of spike characteristics in barley using F3 and F4 families derived from Badia × Komino Cross. Journal of Plant Genetic Research, 3(1), 13-28. (in Farsi)
  13. Khu, Y.H.D. & Monteros, M.J. (2012). High-resolution melting analysis for SNP genotyping and mapping in tetraploid alfalfa (Medicago sativa L.). Molecular Breeding, 29, 489-501.
  14. Kristensen, L. S. & Dobrovic, A. (2008). Direct genotyping of single nucleotide polymorphisms in methyl metabolism genes using probe-free high-resolution melting analysis. Cancer Epidemiol Biomarkers Prev, 17, 1240-1247.
  15. Leida, C., Moser, C., Esteras, C., Sulpice, R., Lunn, J. E., De Langen, F., Monforte, A. J. & Picó, B. (2015). Variability of candidate genes, genetic structure and association with sugar accumulation and climacteric behavior in a broad germplasm collection of melon (Cucumis melo L.). BMC Genetics, 16, 28.
  16. Liew, M., Pryor, R., Palais, R., Meadows, C., Erali, M., Lyon, E. & Wittwer, C. (2004). Genotyping of single-nucleotide polymorphisms by high-resolution melting of small amplicons. Clinical Chemistry (Molecular Diagnostics and Genetics), 50(7), 1156-1164.
  17. Mir Drikvand, R. (2016) Investigation of heritability of morphological traits and genetic diversity among rainfed barley genotypes using molecular and morphological markers. Journal of Plant Genetic Research, 3(2), 69-82. (in Farsi)
  18. Murray, HG. & Thompson, W.F. (1980) Rapid isolation of high molecular weight DNA. Nucleilc Acids Res, 8, 4321-4325.
  19. Oumouloud, A., El-Otmani, M., Chikh-Rouhou, H., Claver, A.G., Torres, R.G., Perl-Treves, R. and lvarez, J.M. (2013). Breeding melon for resistance to Fusarium wilt: recent developments. Euphytica, 192, 155-169.
  20. Perpiñá, G., Esteras, C., Gibon, Y., Monforte, A. J. & Picó, B. (2016). A new genomic library of melon introgression lines in a cantaloupe genetic background for dissecting desirable agronomical traits. BMC Plant Biology, 16(1), p154.
  21. Pitrat, M. (2008). Melon In: Prohens, J. & Nuez, F. (Ed), Handbook of Plant Breeding-Vegetables I (pp. 283-316). Springer, New York.
  22. Raghami, M., Hassandokht, M. R., Zamani, Z., Fattahi Moghadam, M. R. & Kashi, A. (2016). Genetic analysis of fruit sweetness in Iranian melon (Cucumis melo) by complete-diallel crosses. In: Proceedings of the 9th National congress of Horticultural Science, 25-28 Jan. Iran, Ahvaz, Shahid Chamran University. (in Farsi)
  23. Sobhani, A. R. & Hamidi, H. (2015). Melon Breeding and production management. Tak press. (in Farsi)
  24. Suh, S. R., Lee, K-H., Yu, S. H., Shin, H. S., Choi, Y. S. & Yoo, S. N. (2012). Melon Surface Color and Texture Analysis for Estimation of Soluble Solids Content and Firmness. Journal of Biosystems Engineering, 37(4), 252-257.
  25. Taylor, C. F. (2009). Mutation scanning using high-resolution melting. Biochemical Society Transactions, 37(2), 433-437.
  26. Wu, S. B., Franks, T. K., Hunt, P., Wirthensohn, M. G., Gibson, J. P. & Sedgley, M. (2010). Discrimination of SNP genotypes associated with complex haplotypes by high resolution melting analysis in almond: Implications for improved marker efficiencies. Molecular Breeding, 25, 351-357.
  27. Yari, F. (2005). Effect of fruit position on plant and planting Density on melon seed quality of Samsouri. M.Sc Thesis. University of Tabrriz, Iran. (in Farsi)
  28. Yuneji, S., Ghanbarian, D. & Farhadi, A. (2010). Firmness Changes in the melon fruit during ripening and stored. In: Proceedings of the 6th National congress on Agricultural Machinery Engineering & Mechanization. 15-16 Sept., Iran, Karaj, College of Agriculture and Natural Resources, University of Tehran.(in Farsi)