کاربرد آمینولولینیک اسید بر رشد، فعالیت آنتی‌اکسیدانی و تجمع آنتوسیانین در ریزنمونه‌های سیب تحت تنش شوری در شرایط درون‌شیشه‌ای

نوع مقاله: مقاله کامل

نویسندگان

1 دانشجوی سابق دکتری، دانشکده کشاورزی، دانشگاه تبریز، تبریز، ایران

2 دانشیار، دانشکده کشاورزی، دانشگاه تبریز، تبریز، ایران

3 استادیار، دانشکده کشاورزی، دانشگاه تبریز، تبریز، ایران

چکیده

در این پژوهش اثر 5- آمینولولینیک اسید در پنج غلظت 0، 5/2، 5، 10 و 20 میلی­گرم در لیتر در تعدیل تنش شوری (چهار سطح 0، 30، 60 و 90 میلی­مولار) در ریزنمونه‌های سیب پایه  بوداگوسکی نه، بررسی گردید و برخی خصوصیات بیوشیمیایی اندازه­گیری شدند. شاخساره و کالوس بر روی محیط کشت قرار داده شدند و تغییرات فیتوشیمیایی با وجود یا عدم وجود 5- آمینولولینیک اسید در شرایط تنش شوری اندازه­گیری شدند. ریزنمونه‌های شاخساره و کالوس تیمار شده با 5- آمینو لوولینیک اسید (غلظت 5/2 تا 20 میکرومولار) کاهش در فعالیت آنزیم کاتالاز و پراکسیداز و افزایش در فعالیت سوپراکسیددیسموتاز و آسکوربات پراکسیداز را نشان دادند. بیشترین مقدار کلروفیل a وb  ریزنمونه‌های شاخساره در غلظت 10 میکرومولار آمینولولینیک اسید مشاهده شد. هم­چنین تنش شوری نقش مؤثری در افزایش مقدار آنتوسیانین ریزنمونه‌ها ایفا نمود و بیشترین مقدار آن در تنش شوری 90 میلی­مولار به‌دست آمد. افزایش شدت تنش شوری و غلظت 5- آمینو لوولینیک اسید (10-5/2 میکرومولار) در محیط کشت اثر افزایشی بر فعالیت آنتی­اکسیدانی و فنول کل ریزنمونه‌های مورد مطالعه داشت. تنش شوری و آمینو لوولینیک اسید اثر هم­افزایی بر مقدار سنتز فلاونوئیدها نیز داشت.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Effects of aminolevulinic acid on growth, antioxidant activity and anthocyanin accumulation of apple explants under salinity stress in in vitro culture conditions

نویسندگان [English]

  • Fatemeh Zahedzadeh 1
  • Fariborz Zaare Nahandi 2
  • Mohammad Reza Dadpour 2
  • Ali Reza Motallebi-Azar 2
  • Saiedeh Alizadeh Salteh 3
1 Former Ph.D. Student, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
2 Associate Professor, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
3 Assistant Professor, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
چکیده [English]

The present study was carried out to evaluate the effect of 5-aminolevulinic acid (ALA) on physiological and biochemical changes of in vitro-cultured Budaguvsky 9 (Bud 9) apple cultivar under NaCl induced salinity stress. Virus-free shoots and callus  of Bud 9 apple  were cultured on MS medium containing different levels of NaCl (0, 30, 60 and 90 mM) and ALA (0, 2.5, 5, 10 and 20 µM). Four weeks later phytochemical variations of regenerated plantlets with or without NaCl and/or ALA treatments were recorded.  Shoot explants and callus treated with 5-aminolevolinic acid at 2.5 to 20 µM concentrations demonstrated reduction in catalase and peroxidase and enhancement in superoxide dismutase and ascorbate peroxidase enzyme activity. The highest chlorophyll a and b values were observed at shoot explants treated with concentration of 10 μm of aminolevolinic acid. Also, salinity stress was performed an effective role on increasing the amount of anthocyanins of explants and the highest amount was obtained in 90 mM salinity stress. Increasing the intensity of salinity stress and amino acid concentration (2.5-10 μm) in culture media had synergic effect on the antioxidant activity, total phenol as well as flavonoids content of studied explants.  

کلیدواژه‌ها [English]

  • Antioxidant Enzymes
  • biochemical change
  • Bud 9 apple
  • callus
  • explant
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