تأثیر تابش اشعۀ فرابنفش C بر مسیر زیست ساختی استیلبنوئیدها در کشت تعلیقی یاختۀ انگور (Vitis vinifera L. cv. Shahani)

نوع مقاله: مقاله کامل

نویسندگان

1 استاد، دانشکدۀ کشاورزی، دانشگاه بوعلی‌سینا، همدان

2 دانشجوی دکتری، دانشکدۀ کشاورزی، دانشگاه بوعلی‌سینا، همدان

چکیده

استیلبن­های انگور یک خانوادۀ شناخته‌شده از پلی فنول­های گیاهی هستند که فعالیت­های زیستی (بیولوژی) چندی از آن­ها در رابطه با سودمندی­های سلامتی به تأیید رسیده­اند. در این پژوهش، تأثیر محرک اشعۀ فرابنفش C در سه زمان (10، 20 و 30 دقیقه) در ترکیب یا بدون ترکیب با تابش نور مرئی با سطح انرژی بالا (10000 لوکس) روی یک رگۀ (لاین) یاخته‌ای به‌دست‌آمده از میانبر انگور رقم شاهانی (Vitis vinifera cv. Shahani) در قالب آزمایش فاکتوریل با طرح کامل تصادفی بررسی و نتایج کسب‌شده با تیمارهای شاهد مقایسه شد. نتایج نشان دادند، تاریکی می­تواند بیشتر از نور مرئی مسیر سوخت‌وسازی (متابولیکی) مربوط به تولید استیلبنوئیدها را تحریک کند. در میان زمان­های استفاده‌شده از محرک اشعۀ فرابنفش C، ده دقیقه استفاده از اشعۀ فرابنفش C برای تولید مؤثر و تجمع بالای فنول­ها (73/112 میلی­گرم گالیک اسید در هر گرم وزن خشک یاخته) و فلاونوئید­ها (47/150 میلی­گرم کاتکین در هر گرم وزن خشک یاخته) و همچنین استیلبنوئیدها شامل مجموع رسوراترول و فرم گلوکوزیله‌شدۀ آن به نام پیسید (54/7 میکرو­گرم بر میلی­لیتر) بهینه بود. افزون بر این، نتایج نشان داد، یک رابطۀ منفی معنی­دار بین تولید این متابولیت­ها و رشد یاخته وجود دارد. این یافته­ها داده‌های ارزشمندی به‌منظور تولید انبوه کشت­های یاخته‌ای برای تولید این ترکیب‌های خیلی با ارزش در سامانه‌های بیوراکتور ارائه می­کنند.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

The effect of UV-C light irradiation on stilbenoid biosynthetic pathway in grape (Vitis vinifera L. cv. Shahani) cell suspension culture

نویسندگان [English]

  • Mansour Gholami 1
  • Seid Ali Andi 2
1 Professor, Faculty of Agriculture, Bu-Ali Sina University of Hamadan, Iran
2 Ph. D. Candidate, Faculty of Agriculture, Bu-Ali Sina University of Hamadan, Iran
چکیده [English]

Grape stilbenes are well-known family of plant polyphenolics that have been confirmed to have many biological activities in relation to health benefits. In this study, we investigated the effect of UV-C elicitor at three different irradiation periods (10, 20 and 30 min) in combination or not with high-level light irradiation (10000 LUX) on a cell line obtained from the pulp of Vitis vinifera cv. Shahani as Completely Randomized Factorial Design, and compared the results with those of untreated control cultures. Results showed that growing the cells in dark condition can stimulate the metabolic pathway related to bio-production of the stilbenoids more than that of the cells growing under light condition. Among the time courses of UV-C elicitor irradiation, according to our results, irradiation of UV-C for 10 min was optimum for efficient production and higher accumulation of phenolics (112.73 mg GA/g DCW) and flavonoids (150.47 mg catechin/g DCW) as well as stilbenoids including the summation of resveratrol and its glycosylated form piceid (7.54 µg/ml). Furthermore, it was shown that there is a significant negative correlation between production of these metabolites and the cell growth. These data provide valuable information for the future scale up of cell cultures for the production of these very high value compounds in bioreactor systems.

کلیدواژه‌ها [English]

  • Cell suspension culture
  • light irradiation
  • UV-C elicitor
  • Vitis vinifera cv. Shahani
  1. Ali, M., Abbasi, B. H. & Ali, G. S. (2015). Elicitation of antioxidant secondary metabolites with jasmonates and gibberellic acid in cell suspension cultures of Artemisia absinthium L. Plant Cell Tissue and Organ Culture, 120(3), 1099-1106.
  2. Arora, J., Goyal, S. & Ramawat, K.G. (2010). Enhanced stilbene production in cell cultures of Cayratia trifolia through co-treatment with abiotic and biotic elicitors and sucrose. In Vitro Cellular & Developmental Biology-Plant, 46, 430-436.
  3. Cai, Z., Riedel, H., Saw, N.M M.T., Mewis, I., Reineke, K., Knorr, D. & Smetanska, I. (2011). Effects of elicitors and high hydrostatic pressure on secondary metabolism of Vitis vinifera suspension culture. Process Biochemistry, 46, 1411-1416.
  4. Cheng, J. H., Wei, L. Z. & Wu, J. (2015). Effect of Light Quality Selective Plastic Films on Anthocyanin Biosynthesis in Vitis vinifera L. cv. Yatomi Rosa. Journal of Agricultural Science and Technology, 17, 157-166.
  5. Donnez, D., Kim, K. H., Antoine, S., Conreux, A., De Luca, V., Jeandet, P., Clément, C. & Courot, E. (2011). Bioproduction of resveratrol and viniferins by an elicited grapevine cell culture in a 2 L stirred bioreactor. Process Biochemistry, 46, 1056-1062.
  6. Dornenburg, H. & Knorr, D. (1993). Cellular permeabilization of cultured plant tissues by high electric field pulses or ultra high pressure for the recovery of secondary metabolites. Food Biotechnology, 7, 35-48.
  7. Ferri, M., Tassoni, A., Franceschetti, M., Righetti, L., Naldrett, M. J. & Bagni, N. (2009). Chitosan treatment induces changes of protein expression profile and stilbene distribution in Vitis vinifera cell suspensions. Proteomics, 9, 610-624.
  8. Gambourg, O. L., Miller, R. A. & Ojima, K. (1968). Nutrient requirements of suspension cultures of soybean root cells. Experimental Cell Research, 50, 151-156.
  9. Guan, L., Dai, Z., Wu, B.H., Wu, J., Merlin, I., Hilbert, G., Renaud, C., Gome`s, E., Edwards, E., Li, S.H. & Delrot, S. (2016). Anthocyanin biosynthesis is differentially regulated by light in the skin and flesh of white-fleshed and teinturier grape berries. Planta, 243, 23-41.
  10. Hahlbrock, K. (1975). Further Studies on the Relationship between the Rates of Nitrate Uptake, Growth and Conductivity Changes in the Medium of Plant Cell Suspension Cultures. Planta, 124, 311-318.
  11. Hsieh, T. C. & Wu, J. M. (2010). Resveratrol: Biological and pharmaceutical properties as anticancer molecule. BioFactors, 36 (5), 360-369.
  12. Hsieh, T. C. (2009). Antiproliferative Effects of Resveratrol and the Mediating Role of Resveratrol Targeting Protein NQO2 in Androgen Receptorpositive, Hormone-non-responsive CWR22Rv1 Cells. Anticancer Research, 29, 3011-3018.
  13. Kim, Y. C. (2010). Neuroprotective Phenolics in Medicinal Plants. Archives of Pharmacal Research, 33, 1611-1632.
  14. Kiselev, K. V., Shumakova, O. A. & Manyakhin, A.Yu. (2013). Effect of Plant Stilbene Precursors on the Biosynthesis of Resveratrol in Vitis amurensis Rupr. Cell Cultures. Applied Biochemistry and Microbiology, 49(1), 53-58.
  15. Kloypan, C., Jeenapongsa, R., Sri-in, P., Chanta, S., Dokpuang, D., Tip-pyang, S. & Surapinit, N. (2012). Stilbenoids from Gnetum macrostachyum Attenuate Human Platelet Aggregation and Adhesion. Phytotherapy Research, 26, 1564-1568.
  16. Krasnow, M.N. & Murphy, A.T. (2004). Polyphenol Glucosylating Activity in Cell Suspensions of Grape (Vitis vinifera). Journal of Agricultural and Food Chemistry, 52, 3467-3472.
  17. Krisa, S., Larronde, F., Budzinski, H., Decendit, A., Deffieux, G. & Merillon, J. M. (1999). Stilbenes production by Vitis vinifera cell suspension cultures: methyl jasmonate induction and 13C biolabeling. Journal of Natural Products, 62, 1688-1690.
  18. Lijavetzky, D., Almagro, L., Belchi-Navarro, S., Martínez-Zapater, J. M., Bru, R. & Pedreño, M. A. (2008). Synergistic effect of methyljasmonate and cyclodextrin on stilbene biosynthesis pathway gene expression and resveratrol production in Monastrell grapevine cell cultures. BMC Research Notes, 1, 132.
  19. Liscombe, D. K. (2008). Discovery of Novel Alkaloid Biosynthetic Genes Using Biochemical Genomics. Ph.D. Thesis. Department of Biological Sciences, Calgary University, Canada.
  20. Liu, W., Liu, C., Yang, C., Wang, L. & Li, S. (2010). Effect of grape genotype and tissue type on callus growth and production of resveratrols and their piceids after UV-C irradiation. Food Chemistry, 122, 475-481.
  21. Mewis, I., Smetanska, I. M., Müller, C. T. & Ulrichs, C. (2011). Specific Poly-phenolic Compounds in Cell Culture of Vitis vinifera L. cv. Gamay Fréaux. Applied Biochemistry and Biotechnology, 164, 148-161.
  22. Morel, G. (1970). Le probleme de la transformation tumorale chez les végétaux. Physiologie Vegetale, 8, 189-191.
  23. Murashige, T. & Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15, 472-497.
  24. Nagella, P. & Murthy, H. N. (2010). Establishment of cell suspension cultures of Withania somnifera for the production of withanolide A. Bioresource Technology, 101, 6735-6739.
  25. Qu, J., Zhang, W. & Yu, X. (2011). A combination of elicitation and precursor feeding leads to increased anthocyanin synthesis in cell suspension cultures of Vitis vinifera. Plant Cell Tissue and Organ Culture, 107, 261-269.
  26. Roat, C. & Ramawat, K. G. (2009). Morphactin and 2iP markedly enhance accumulation of stilbenes in cell cultures of Cayratia trifolia (L.) Domin. Acta Physiologiae Plantarum, 31, 411-414.
  27. Sae-Lee, N., Kerdchoechuen, O. & Laohakunjit, N. (2014). Enhancement of Phenolics, Resveratrol and Antioxidant Activity by Nitrogen Enrichment in Cell Suspension Culture of Vitis vinifera. Molecules, 19, 7901-7912.
  28. Santamaria, A. R., Mulinacci, N., Valletta, A., Innocenti, M. & Pasqua, G. (2011). Effects of Elicitors on the Production of Resveratrol and Viniferins in Cell Cultures of Vitis vinifera L. cv Italia. Journal of Agricultural and Food Chemistry, 59, 9094-9101.
  29. Saw, N. M. M. T., Riedel, H., Cai, Z., Ku¨tu¨k, O. & Smetanska, I. (2012). Stimulation of anthocyanin synthesis in grape (Vitis vinifera) cell cultures by pulsed electric fields and ethephon. Plant Cell Tissue and Organ Culture, 108, 47-54.
  30. Shi, L., Cao, S., Chen, W. & Yang, Z. (2014). Blue light induced anthocyanin accumulation and expression of associated genes in Chinese bayberry fruit. Scientia Horticulturae, 179, 98-102.
  31. Singleton, V. L., Orthofer, R. & Lamuela-Raventos, R. M. (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu Reagent. Methods in Enzymology, 299, 152-178.
  32. Van der Plas, L. H. W., Eijkelboom, C. & Hagendoorn, M. J. M. (1995). Relation between primary and secondary metabolism in plant cell suspensions. Plant Cell Tissue and Organ Culture, 43, 111-116.
  33. Vuong, T. V., Franco, C. & Zhang, D. (2014). Treatment strategies for high resveratrol induction in Vitis vinifera L. cell suspension culture. Biotechnology Reports, 1-2, 15-21.
  34. Waffo-Téguo, P., Krisa, S., Pawlus, A. D., Richard, T., Monti, J.P. & Merillon, J. M. (2013). Phytochemistry, Botany and Metabolism of Alkaloids, Phenolics and Terpenes. In: K.G. Ramavat & J.M. Mérillon (Eds), Grapevine Stilbenoids: Bioavailability and Neuroprotection. (p. 2277) Springer Science.   
  35. Xu, A., Zhan, J. C. & Huang, W. D. (2015). Effects of ultraviolet C, methyl jasmonate and salicylic acid, alone or in combination, on stilbene biosynthesis in cell suspension cultures of Vitis vinifera L. cv. Cabernet Sauvignon. Plant Cell Tissue and Organ Culture, 122(1), 197-211.
  36. Yin, Y., Borges, G., Sakuta, M., Crozier, A. & Ashihara, H. (2012). Effect of phosphate deficiency on the content and biosynthesis of anthocyanins and the expression of related genes in suspension-cultured grape (Vitis sp.) cells. Plant Physiology and Biochemistry, 55, 77-84.
  37. Yuan, Y. J., Wei, Z. J., Miao, Z. Q. & Wu, J. C. (2002). Acting paths of elicitors on Taxol biosynthesis pathway and their synergistic effect. Biochemical Engineering Journal, 10, 77-83.
  38. Zhang, W., Curtin, C., Kikuchi, M. & Franco, C. (2002). Integration of jasmonic acid and light irradiation for enhancement of anthocyanin biosynthesis in Vitis vinifera suspension cultures. Plant Science, 162, 459-468.
  39. Zhao, J., Davis, L. C. & Verpoorte, R. (2005). Elicitor signal transduction leading to production of plant secondary metabolites. Biotechnology Advances, 23, 283-333.
  40. Zhou, Y. & Singh, B. R. (2004). Effect of Light on Anthocyanin Levels in Submerged, Harvested Cranberry Fruit. Journal of Biomedicine and Biotechnology, 5, 259-263.