نوع مقاله : مقاله پژوهشی
نویسندگان
1 دانشجوی کارشناسی ارشد، دانشکدۀ کشاورزی دانشگاه تربیت مدرس، تهران، ایران
2 استادیار، دانشکدۀ کشاورزی دانشگاه تربیت مدرس، تهران، ایران
چکیده
گلمحمدی یکی از مهمترین گیاهان معطر در ایران و جهان است. اسانس استخراجشده از گلبرگ این گل در داروسازی، صنایع آرایشی و بهداشتی و همچنین غذایی استفاده میشود. استفاده از روشهای معمول برای افزایش این گیاه با دشواریهایی چون انتقال عاملهای آلودگی و محدود بودن گیاه مادری همراه است. با توجه به برتریهای روشهای افزایش درون شیشهای نسبت به روشهای رایج، این تحقیق بهمنظور بررسی تأثیر انواع مختلف محیط کشت با سطوح هورمونی متفاوت برای بهبود افزایش درون شیشهای و کنترل سبزردی (کلروز) نوساقهها انجام شد. محیط کشتهای MS و MS تغییریافته (mMS0، mMS1، mMS2، mMS3، mMS4و mMS5) در این پژوهش استفاده شد. قطعههای گرهای حاوی 1 تا 2 جوانۀ جانبی در محیط کشت MS با سطوح مختلف هورمونی برای استقرار کشت شدند. برای نوساقهزایی به محیط کشتهای حاوی غلظتهای 1 و 2 میلیگرم بر لیتر BAP و 2/0 میلیگرم بر لیتر IBA منتقل شده و آنگاه برای ریشهزایی در محیط کشتهای 1/2 MS و LS با سطوح 1 و 2 میلیگرم بر لیتر IBA کشت شدند. بهترین تیمار برای افزایش شمار نوساقههای گلمحمدی محیط کشت مایع mMS0 حاوی 5/1 میلیگرم بر لیتر BAP به همراه 2/0 میلیگرم بر لیتر IBA بود. بیشترین میزان سبزینۀ (کلروفیل) برگها نیز در محیط کشت mMS0 مشاهده شد. همچنین بیشترین درصد ریشهزایی مربوط به ژنوتیپ گالیکا در محیط کشت 1/2MS دارای 2 میلیگرم بر لیتر IBA بود.
کلیدواژهها
عنوان مقاله [English]
Improvement of in vitro micro propagation of Rosa damascena genotypes
نویسندگان [English]
- Mohamad Omidi 1
- Abbas Yadollahi 2
- Noorollah Ahmadi 2
- Farhad Beyranvand 1
1 M. Sc. Student and Assistant Professor, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran
2 Assistant Professor, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran
چکیده [English]
Rosa damascena is one of the most important Aromatic plants in the world. The extracted oil from the petals of the flowers is used in the pharmaceutical, cosmetic and food industries. Traditional methods to propagate this plant have problems such as transfer of pollution agents and limited native plants. Due to more advantages of the in vitro proliferation in compared to traditional methods, this study designed to evaluate impression different medium cultures with diverse hormone levels to improve the proliferation and control of shoots chlorosis. In this research we used medium cultures of MS and mMS (mMS0, mMS1, mMS2, mMS3, mMS4 and mMS5). In order to establishment in MS medium, nodal segments containing 1 or 2 lateral buds were cultured. For regenerating the shoots, mediums with different concentrations of 1 and 2 mg/l BAP and 0/2 mg/l IBA were supplied, then plantlets were transferred to the rooting medium (1/2 MS and LS) with different levels of IBA (1 and 2 mg/l). The best treatment to increase the number of rose shoots was mMS medium containing 1.5 mg/l of BAP and 0.2 mg/l of IBA. The highest chlorophyll content of leaves was observed in mMS medium. Gallica plantlets produced the highest percentage of rooting on 1/2 MS with 2 mg/l of IBA.
کلیدواژهها [English]
- BAP
- Chlorophyll
- Hormone
- IBA
- Micropropagation
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