علوم باغبانی ایران

علوم باغبانی ایران

شناسایی، اهلی‌کردن و مقایسۀ عملکرد نژاد بومی قارچ صدفی (Pleurotus spp) با نژاد تجاری

نوع مقاله : مقاله پژوهشی

نویسندگان
فاطمه رئیسی 1
غلام علی پیوست 2
معظم حسن پور اصیل 2
جمال علی الفتی 3
سید اکبر خداپرست 4
1 کارشناس ارشد، گروه علوم باغبانی، دانشگاه گیلان
2 استاد، گروه علوم باغبانی، دانشگاه گیلان
3 استادیار گروه علوم باغبانی، دانشگاه گیلان
4 دانشیار گروه گیاه‌پزشکی، دانشگاه گیلان
10.22059/ijhs.2015.56916
چکیده
در این پژوهش ابتدا دو نژاد قارچ صدفی که از نظر صفات ظاهری تفاوت نشان می‏دادند از جنگل‌های گیلان جمع‌آوری و سپس در محیط کشت MEA کاشته شدند. پس از تکمیل رشد میسیلیوم در ظروف کشت، اسپان قارچ تهیه ‌و کشت قارچ در بستر کاه برنج انجام شد. در‌نهایت پس از برداشت محصول، شناسایی گونه‏های وحشی با استفاده از روش PCR و روش‏های میکرو و ماکروسکوپی صورت گرفت. بر این اساس گونه‏های شناسایی‌شده شامل Pleurotus ostreatus و Pleurotus pulmonariusبودند. نتایج این پژوهش نشان داد که کوتاه‏ترین زمان تشکیل پین‏هد مربوط به نژاد تجاری فلوریدا (8/14روز) و بیشترین میزان پروتئین (24/26 درصد) و نیتروژن (77/9 درصد) مربوط به نژاد استراتوس بود. در سایر صفات اندازه‏گیری شده تفاوت معنا‏داری بین نژادهای مختلف وجود نداشت.
کلیدواژه‌ها
قارچ صدفی‌
کشت بافت
PCR
Pleurotus ostreatus
Pleurotus pulmonarius

عنوان مقاله English

Identification, domestication and yield of local Pleurotus species in comparison with commercial oyster mushroom

نویسندگان English

Fatemeh Raeisi 1
Gholamali Peyvast 2
Moazam Hassanpour Asil 2
Jamal Ali Olfati 3
Seyed Akbar Khodaparast 4
1 Former M.Sc. Student, Horticultural Department, University of Guilan, Iran
2 Professor, Horticultural Department, University of Guilan, Iran
3 Assistant Professor, Horticultural Department, University of Guilan, Iran
4 Associate Professor, Department of Plant Protection, University of Guilan, Iran
چکیده English

In present study two species of Pleurotus with different morphological characteristics were collected from Guilan forest and then cultured on MEA medium. Spawn was prepared on wheat grains and finally all strains were cultivated on rice straw. Identification of species was performed by PCR, microscopic and macroscopic characters. Results revealed that our local strains are known as Pleurotus ostreatus and Pleurotus pulmonarius. Minimum days required for pinhead formation were observed in P. florida (14.8 days). The highest protein and nitrogen content was found in P. florida (26.24 and 9.77%). In other characteristics, there were no any significant differences between strains.

کلیدواژه‌ها English

oyster mushroom
PCR
Pleurotus ostreatus
Pleurotus pulmonarius
Tissue culture
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  • تاریخ دریافت 14 مرداد 1392
  • تاریخ بازنگری 05 مرداد 1393
  • تاریخ پذیرش 04 شهریور 1393