شناسایی، اهلی‌کردن و مقایسۀ عملکرد نژاد بومی قارچ صدفی (Pleurotus spp) با نژاد تجاری

نوع مقاله: مقاله کامل

نویسندگان

1 کارشناس ارشد، گروه علوم باغبانی، دانشگاه گیلان

2 استاد، گروه علوم باغبانی، دانشگاه گیلان

3 استادیار گروه علوم باغبانی، دانشگاه گیلان

4 دانشیار گروه گیاه‌پزشکی، دانشگاه گیلان

چکیده

در این پژوهش ابتدا دو نژاد قارچ صدفی که از نظر صفات ظاهری تفاوت نشان می‏دادند از جنگل‌های گیلان جمع‌آوری و سپس در محیط کشت MEA کاشته شدند. پس از تکمیل رشد میسیلیوم در ظروف کشت، اسپان قارچ تهیه ‌و کشت قارچ در بستر کاه برنج انجام شد. در‌نهایت پس از برداشت محصول، شناسایی گونه‏های وحشی با استفاده از روش PCR و روش‏های میکرو و ماکروسکوپی صورت گرفت. بر این اساس گونه‏های شناسایی‌شده شامل Pleurotus ostreatus و Pleurotus pulmonariusبودند. نتایج این پژوهش نشان داد که کوتاه‏ترین زمان تشکیل پین‏هد مربوط به نژاد تجاری فلوریدا (8/14روز) و بیشترین میزان پروتئین (24/26 درصد) و نیتروژن (77/9 درصد) مربوط به نژاد استراتوس بود. در سایر صفات اندازه‏گیری شده تفاوت معنا‏داری بین نژادهای مختلف وجود نداشت.

کلیدواژه‌ها


عنوان مقاله [English]

Identification, domestication and yield of local Pleurotus species in comparison with commercial oyster mushroom

نویسندگان [English]

  • Fatemeh Raeisi 1
  • Gholamali Peyvast 2
  • Moazam Hassanpour Asil 2
  • Jamal Ali Olfati 3
  • Seyed Akbar Khodaparast 4
1 Former M.Sc. Student, Horticultural Department, University of Guilan, Iran
2 Professor, Horticultural Department, University of Guilan, Iran
3 Assistant Professor, Horticultural Department, University of Guilan, Iran
4 Associate Professor, Department of Plant Protection, University of Guilan, Iran
چکیده [English]

In present study two species of Pleurotus with different morphological characteristics were collected from Guilan forest and then cultured on MEA medium. Spawn was prepared on wheat grains and finally all strains were cultivated on rice straw. Identification of species was performed by PCR, microscopic and macroscopic characters. Results revealed that our local strains are known as Pleurotus ostreatus and Pleurotus pulmonarius. Minimum days required for pinhead formation were observed in P. florida (14.8 days). The highest protein and nitrogen content was found in P. florida (26.24 and 9.77%). In other characteristics, there were no any significant differences between strains.

کلیدواژه‌ها [English]

  • oyster mushroom
  • PCR
  • Pleurotus ostreatus
  • Pleurotus pulmonarius
  • Tissue culture
  1. Adamovic, M., Grubic, G., Milenkovic, I., Jovanovic, R., Prptic, R., Sretenovic, L. & Stoicevic, L. J. (1997). The biodegradation of wheat straw by Pleurotus ostreatus mushroom and its use in cattle feeding. Animal Food Science Technology, 71, 357-362.
  2. Alam, N., Amin, R., Khan, A., Ara, I., Shim, M., Lee, M. & Lee,T. (2008). Nutritional analysis of cultivated mushrooms in Bangladesh –Pleurotus ostreatus, Pleurotus sajor-caju, Pleurotus florida and Calocybe indica. Mycobiology, 36(4), 228-232.
  3. Asghar, R., Tariq , M. & Rehaman, T. (2007). Propagation of Pleurotus sajor-caju (Oyster mushroom) through tissue culture. Pakistan Journal of Botany, 39(4), 1383-1386.
  4. Baysal, E., Peker, H., Yalinkilic, M. & Temiz , A. (2003). Cultivation of oyster mushroom on waste paper with some added supplementary materials. Bioresource Technology, 89, 95-97.
  5. Bonatti, M., Karnopp, P., Soares, H. M. & Furlan, S. A. (2004). Evaluation of Pleurotus ostreatus and Pleurotus sajor-caju nutritional characteristics when cultivated in different lignocellulosic wastes. Food chemistry, 88, 425-428.
  6. Dus, N. & Mukherjee, M. (2007). Cultivation of Pleurotus ostreatus on weed plants. Bioresource Technology. 98, 2723-2726.
  7. Gbolagade, J., Ajayi, A., Oku, I. & Wankasi, D. (2006). Nutritive value of common wild edible mushrooms from southern nigeria. Global Journal of Biotechnology & Biochemistry, 1(1), 16-21.
  8. Han, J. (1999). The influence of photosyntheticbacteria treatments on the crop yield, dry matter content, and protein cotent of the mushroom Agaricus bisporus. Scientia Horticulturae, 82, 171-178.
  9. Jonathan, S. G. & Fasidi, I. O. (2003). Studies on Psathyerella atroumbonata (Pegler), a Nigerian edible fungus. Food Chemistry, 81, 481-484.
  10. Khan, Md. A., Ruhul Amin, S. M., Nazim Uddin, Md., Tania, M. & Alam, N. (2008). Comparative study of the nutritional composition of oyster mushrooms cultivated in Bangladesh. Bangladesh Journal of Mushroom, 2(1), 9-14.
  11. Norouzi, A., Peyvast, Gh. & Olfati, J.A. (2008). Oilseed rape straw for cultivation of oyster mushroom. Maejo International Journal of Science and Technology, 2(3), 502-507.
  12. Obodai, M., Cleland-Okine, J. & Vowotor, K. A. (2002). Comparative study and yield of Pleurotus ostreatus mushroom on different lignocellulosic by-products. Microbiology and Biotechnology, 53, 42-47.
  13. Olfati, J.A. & Peyvast, Gh. (2008). Lawn clippings for cultivation of oyster mushroom. International Journal of Vegetable Science, 14(2), 98-103.
  14. Olfati. J.A., Peyvast, Gh. & Mami, Y. (2009). Identification and chemical properties of popular wild edible mushrooms from northern Iran. Journal of Horticulture and Forestry, 1(3), 48-51.
  15. Peyvast, Gh., Olfati, J.A., Kariminia, A. & Fallah, A. (2009). Bradyrhizobium bacteria increase oyster mushroom quality. Journal of pure and Applied Microbiology, 3(2), 421-424.
  16. Royse, D. J., Rhodes, T. W., Ohga, S. & Sanchez., J. E. (2004). Yield, mushroom size and time to production of pleurotus cornucopiae (oyster mushroom) grown on switch grass substrate spawned and supplemented at various rates. Bioresource Technology, 91, 85-91.
  17. Singh, D. & Singh, S. P. (2005). Modern Mushroom Cultivation. Updesh Purohit for Agrobios. Pp. 86-93.
  18. Yildiz, S., Yildiz, U. G., Gezer, E. D. & Temiz, A. (2002). Some lignocellulosic wastes used as raw material in cultivation of the pleurotus ostreatus culture mushroom. Process Biochemistry, 38, 301-306.
  19. Zhang, R., Li, X. & Fadel, J. G. (2001). Oyster mushroom cultivation with rice and wheat straw. Bioresource Technology, 82, 277-284.