تاثیر ماده آلی و نوع محیط کشت بر خصوصیات رویشی، فعالیت آنزیمی و میزان اسانس بادرنجبویه ‏‏(‏Melissa officinalis L.‎‏) در شرایط تنش شوری

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری علوم باغبانی، واحد یاسوج، دانشگاه آزاد اسلامی، یاسوج، ایران

2 دانشیار علوم باغبانی، واحد جهرم، دانشگاه آزاد اسلامی، جهرم، ایران

3 دانشیار علوم باغبانی، واحد یاسوج، دانشگاه آزاد اسلامی، یاسوج، ایران

چکیده

به منظور مطالعه تاثیر ماده آلی و نوع محیط کشت بر خصوصیات رویشی، فعالیت آنزیمی و میزان اسانس گیاه دارویی بادرنجبویه در شرایط تنش شوری، آزمایشی به صورت فاکتوریل در قالب طرح کاملاً تصادفی با سه تکرار در شهرستان جهرم استان فارس انجام شد. فاکتور اول بستر کشت در سه سطح (ترکیب کمپوست+ خاک زراعی، ورمی­کمپوست+ خاک زراعی و کمپوست+ ورمی­کمپوست+ خاک زراعی (همگی به نسبت های مساوی))، فاکتور دوم ماده­آلی در دو سطح (اسید هیومیک و میکروارگانیسم­های موثر ((EM (با غلظت 5 در هزار)) و فاکتور سوم شوری در سه سطح  (515، 3656­ و7312 میکروموس بر سانتی­متر) بود. نتایج نشان داد با افزایش شوری، میزان پرولین و فعالیت آنزیم‌های کاتالاز و پراکسیداز افزایش و ارتفاع گیاه، تعداد برگ، کلرفیل و درصداسانس کاهش یافت. اثر افزودن توام ورمی‌کمپوست و کمپوست به خاک بر صفات رشدی و فیزیولوژیکی، موثرتر از هر یک از این دو ماده به تنهایی بود. به­طوری کلی کاربرد ماده آلی و میکروارگانیزم های موثر در بستر کشت حاوی خاک+ کمپوست+ ورمی­کمپوست اثرات منفی شوری بر بادرنجبویه را کاهش داد و کاربرد ماده آلی و میکروارگانیزم های موثر می تواند در شرایط تنش شوری باعث بهبود تحمل و افزایش خصوصیات رشدی و افزایش  محتوی درصد اسانس این گیاه دارویی گردد.

کلیدواژه‌ها


عنوان مقاله [English]

The effect of organic matter and type of culture medium on vegetative ‎characteristics, enzymatic activity and essential oil of lemon balm (Melissa ‎officinalis L.) under salinity stress

نویسندگان [English]

  • Shima Rahmanian 1
  • Abdolhossein Aboutalebi Jahroomi 2
  • Mehdi Hosseinifarahi 3
1 Ph. D. Candidate of Horticultural Science, Yasooj Branch, Yasooj, Iran
2 Associate Professor of Horticulture, Jahroom Branch, Islamic Azad University, Jahroom, Iran
3 Associate Professor of Horticulture, Yasooj Branch, Islamic Azad University, Yasooj, Iran
چکیده [English]

In order to study the effect of organic matter and type of culture medium on vegetative characteristics, enzymatic activity and essential oil of lemon balm under salinity stress, a factorial experiment based on completely randomized design with three replications in Jahrom city of Fars province carried out. The first factor was the medium culture at three levels (compost + soil, vermicompost + soil and compost + vermicompost + soil (all in equal proportions)), the second factor was organic matter at two levels (humic acid (HA) and effective microorganisms (EM)( concentration of 5 per thousand) and the third factor was salinity at three levels (515, 3656 and 7312 μm / cm). The results showed that with increasing salinity, proline content and activity of catalase and peroxidase enzymes increased and plant height, leaf number, chlorophyll and percentage of essential oil decreased. Co-addition of vermicompost and compost to soil on growth and physiological traits was more effective than either of these two substances alone. In general, the use of organic matter and effective microorganisms in the culture medium containing soil+ compost+ vermicompost reduced the negative effects of salinity on lemon balm and the use of organic matter and effective microorganisms can improved plant tolerance under salinity stress conditions and increased the growth characteristics and essential oil content of this medicinal plant.

کلیدواژه‌ها [English]

  • Catalase
  • fresh and dry weight
  • humic acid
  • sodium chloride
  • vermicompost
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