ارزیابی میزان تحمل به یخ‌زدگی پنیرباد (‏Withania coagulans Dun‏) تحت شرایط کنترل‌شده

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری، گروه کشاورزی دانشگاه آزاد اسلامی واحد شیروان، ایران

2 دانشیار، بخش تحقیقات علوم زراعی و باغی، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی خراسان رضوی، مشهد، ایران

3 استادیار، گروه زراعت و اصلاح نباتات، دانشگاه آزاد اسلامی واحد شیروان، ایران ‏

چکیده

به‌منظور بررسی میزان تحمل به یخ‌زدگی گیاه پنیرباد آزمایشی در قالب طرح کاملاً تصادفی با 4 تکرار و نه تیمار دمایی (صفر، 3-، 6-، 9-، 12-، 15-، 18- و 21- درجه سانتی‌گراد و تیمار شاهد ۲5 درجه سانتی‌گراد) انجام شد. صفات مورد مطالعه شامل درصد نشت یونی، پایداری غشای سلولی، محتوای پرولین برگ، کلروفیل فلورسانس، درجه سبزینگی، دمای 50 درصد کشندگی بر اساس درصد بقا (LT50su) و دمای 50 درصد کشندگی بر اساس نشت الکترولیت‌ها (LT50el) بود. نتایج نشان داد اثر تنش یخ‌زدگی بر تمامی صفات مورد مطالعه در سطح احتمال 1 درصد معنی‌دار بود. با افزایش تنش یخ‌زدگی نشت یونی افزایش و پایداری غشای سلولی کاهش نشان دادند. میزان کلروفیل فلورسانس برگ با افزایش تنش کاهش یافت، به طوری که در دماهای پایین تر از 9- درجه سانتی‌گراد به کمترین مقدار خود رسید. درجه سبزینگی تا 6- درجه سانتی‌گراد با شیب شدید و در کمتر از آن با شیب ملایم تر کاهش یافت. بیشترین محتوای پرولین مربوط به تیمار 6- درجه سانتی‌گراد بود که نسبت به تیمار شاهد 53 درصد افزایش یافت. با کاهش دما درصد بقا کاهش یافت، به طوری که در دماهای زیر 9- درجه سانتی‌گراد به کلی گیاهان از بین رفتند. همچنین LT50el  و LT50su  به ترتیب در دمای 153/10- و 958/8- درجه سانتی‌گراد اتفاق افتاد. در نهایت، یافته ها در این تحقیق نشان دادند که گیاه دارویی پنیرباد تنش یخ‌زدگی 6- درجه سانتی‌گراد را به طور قابل قبولی تحمل می‌نماید.

کلیدواژه‌ها

عنوان مقاله [English]

Evaluation of freezing stress in withania (Withania coagulans‏ ‏Dun) under controlled ‎conditions

نویسندگان [English]

  • Tayebeh Zendedel 1
  • Ebrahim Ganji Moghadam 2
  • Maryam Tatari 3
1 Ph.D. Candidate, Department of Agricultural, Shirvan Branch, Islamic Azad University, Iran‎
2 Associate Professor, Crop and Horticultural Science Research Department, Khorasan Razavi Agricultural Resources Research and ‎Education Center, Mashhad, Iran
3 Assistant Professor, Department of Agricultural, Shirvan, Branch, Islamic Azad University, Iran
چکیده [English]

In order to investigate freezing tolerance in withania under controlled conditions, a completely randomized design was carried out with four replications and nine temperature treatments (0, -3, -6, -9, -12, -15, -18, -21 °C and control treatment 25°C). Studied traits included electrolyte leakage, cell membrane stability, proline content, chlorophyll fluorescence, chlorophyll degree, 50% lethal temperature based on survival (LT50su) and 50% lethal temperature based on electrolyte leakage (LT50el). Results showed that the effect of freezing stress on all traits was significant at a probability of one percent. Electrolyte leakage showed increased and cell membrane stability decreased. The leaf chlorophyll fluorescence decreased with increasing freezing stress, so that it reached its lowest point at temperatures below -9°C. Chlorophyll content index was also affected by the effect of freezing stress, which decreased at a steep slope of 0 to -6°C and lowered to -6 °C with lower slope. The highest content of proline was related to treatment at -6°C, which increased by 53% compared to control treatment. By decreasing the temperature, the survival rate decreased, so that the plants disappeared at temperatures below -9°C. Also, LT50el and LT50su occurred at temperatures of -10.153 and -8.958°C, respectively. Finally, the findings in this study showed that the medicinal plants withania can be tolerate at -6°C.

کلیدواژه‌ها [English]

  • Chlorophyll fluorescence
  • proline
  • stress
  • survival percentage

مراجع

  1. Amirghasemi, T. (2003). Frosting of plants (frost, injuries, prevention) (1th ed.). Ayndangan House. (In Farsi).
  2. Asghari, B., Rehman, A. & Matthias, W. (2009). Altitudinal variation in the content of protein, proline, sugar and ABA in the Alpine herbs from Hunza Valley, Pakistan. Pakistan Journal of Botany, 41(4), 1593-1602.
  3. Ashraf, M. & Foolad, M. R. (2007). Roles of glycine betaine and proline in improving plant abiotic stress resistance. Environmental and Experimental Botany, 59, 206-216.
  4. Barranco, D., Ruiz, & Gomez-del-Campo, M. (2005). Frost tolerance of eight olive cultivars. Horticultural Science, 40, 558-560.
  5. Bates, L. , Walden, R. P. & Teave, I. D. (1973). Rapid determination of free proline for water stress studies. Plant and Soil, 39, 205-208.
  6. Behandari, S. R., YongHyeon, K. & JunGu, L., (2018). Detection of temperature stress using chlorophyll fluorescence parameters and stress-related chlorophyll and proline content in paprika (Capsicum annuum L.) seedlings. Horticultural Science and Technology, 36 (5), 619-629.
  7. Borumand Reza Zadeh, Z., Nezami, A. & Nezami, S. (2014). Evaluation of Freezing Tolerance of Three Ajowan (Trachyspermum ammi Sprague) Ecotypes in Controlled Conditions. Iranian Journal of field crops, 11(1), 121-130. (In Farsi).
  8. Cardona, C. , Duncan, R R. & Lindstorm, O. (1997). Low temperature tolerance assessment in paspalum. Crop Science, 37, 1283-1291.
  9. Cavender-Bares, (2007). Chilling and freezing stress in live oaks (Quercus section Virentes): intraand interspecifc variation in PS II sensitivity corresponds to latitude of origin. Photosynthesis Research, 94 (2-3), 437-453.
  10. Chen, Y., Zhang, M., Chen, T. Zhang, & L, A. n. (2006). The aelationship between seasonal changes in Anti-oxidative system and freezing tolerance in the leaves of evergreen woody plants of sabina. South African Journal of Botany, 72 (2), 272-279.
  11. Dashti, M., Kafi, M., Tavakoli, H., Mirza, M. & Nezami, A. (2016). Effects of freezing stress on Morpho-physiological indices and chlorophyll fluorescence of Salvia leriifolia in seedling stage. Journal of Plant research. Iranian Journal of Biology, 28(5), 962-973. (In Farsi).
  12. Davik,, Koehler, G., From, B., Torp, T., Rohloff, J., Eidem, P., Wilson, R. C., Sønsteby, A., Randall, S. K. & Alsheikh, M. (2013). Dehydrin, alcohol dehydrogenase, and central metabolite levels are associated with cold tolerance in diploid strawberry (Fragaria spp.). Planta, 237(1), 265-277.
  13. Eugenia, M., Nunes, S. & Ray Smith, G. (2003). Electrolyte leakage assay capable of quantifying freezing resistance in Rose Clover. Crop Science, 43, 1349-1357.
  14. Ghahremani, A., Ganji moghaddam, E., Tatari, M. & Khosroyar, S. (2020). Effect of type and concentration of growth regulators on the proliferation and marcotting of Withania coagulans medical plant. Iranian Journal of Horticultural Science,51(2), 287-294. (In Farsi).
  15. Hana, B. & Bischofa, C. (2004). Direct cell injury associated with eutectic crystallization during freezing. Cryobio, 48, 8-21.
  16. Hanim, H. & Nesrin, Y. (2009). Does climate change have an effect on proline accumulation in pomegranate (Punica granatum L.) fruits. Scientific Research and Essay, 4(12), 1543-
  17. Hao,, Arora R., Yadav, A. K. & Joshee N. (2009). Freezing tolerance and cold acclimation in guava (Psidium guajava L.). HortScience, 44(5), 1258-1266.
  18. Ibrahim, M. M. & Bafeel, S. O. (2008). Photosynthetic efficiency and pigment contents in alfalfa seedling subjected to dark and chilling condition. International Journal of Agriculture and Biology, 10(3), 306-310.
  19. Kheir khah, T., Nezami, A., Kafi, M. & Asadi, GH. (2014). Evaluation of cold tolerance in field grown mentha (Mentha piperita‎‏)‎ under ‎laboratory conditions by electrolyte leakage test. Iranian Journal of Field Crops Research, 13(2), 269-277. (In Farsi).
  20. Khorsandi, t., Nezami. A., Kafi. M. & Goldani, M. (2013). Effects of spring late frost on black seed (Nigella sativa) under controlled conditions. Iranian Journal of Field Crops Research, 12, 665-676. (In Farsi).
  21. Li, H., Qiang, S. & Qian, Y. (2008). Physiological response of different croftonweed (Eupatorium adenophorum) populations to low temperature. Weed Science, 56, 196-
  22. Maleki, H., Zakizade, H., Hamidoghli, Y., Biglui, M. & Chamani, E. (2018). Ornamental potential and freezing tolerance of six Thymus spp. species as ground-covering plants in the landscape. Zemdirbyste-Agriculture, 105, 79-88.
  23. Modhan, M. M., Narayanan, S. L. & Ibrahim, S. M. (2000). Chlorophyll stability indexes (CSI): its impacts on salt tolerance in rice. International Rice Research. Notes, 25(2), 38-40.
  24. Murray, G. , Eser, D., Gusta, L. V. & Eteve, G. (1988). Winter hardiness in pea, lentil, faba bean and chickpea. In: Summerfield R.J., (Ed), World crops: Cool Season Food Legumes. (pp. 831-843.) Kluwer Academic Publishers, the Netherlands.
  25. Nathalie, V. & Christian, H. (2008). Proline accumulation in plants: a review. Amino Acids, 35, 753-759.
  26. Negi, M. , Singh, A. & Lakshmikumaran, M. (2000). Genetic variation and relationship among and within Withania species as revealed by AFLP markers. Genome, 43(6), 975-980.
  27. Nezami, A., Haj Mohamadniya Ghali Baf, K. & Kamandi, (2011). Evaluation of sugar beet (Beta vulgaris L.) To freezing tolerance under controlled conditions. Environmental Stresses in Crap Sciences, 3(2), 177-187. (In Farsi).
  28. Oliveira, & Penuelas, J. (2000). Comparative photochemical and phenomorphological responses to winter stress of an evergreen (Quercus ilex L.) and a semi-deciduous (Cistus albidus L.) Mediterranean woody species. Acta Oecologica, 21, 97-107.
  29. Panwar, J. & Tarafdar, J. (2006). Distribution of three endangered medicinal plant species and their colonization with arbuscular mycorrhizal fungi. Journal of Arid Environments, 65(3), 337-350.
  30. Percival, & Henderson, A. (2003). An assessment of the freezing tolerance of urban trees using chlorophyll fluorescence. Journal of Horticultural Science and Biotechnology, 78, 254-260.
  31. Rashed Mohasel, H., Nezami, A., Bagheri, A., Hajmohammadnia, K. & Bannayan, M. (2009). Evaluation of freezing tolerance of two fennel (Foeniculum vulgar L.) ecotypes under controlled conditions. Journal of Herbs, Spices & Medicinal Plants, 15, 131-140.
  32. Rezvan Beydokhti, S., Nezami, A., Kafi, M. & Khazaei, R. (2011). Effects of freezing stress on electrolyte leakage of persian shallot (Allium altissimum ) under controlled conditions. Agroecology, 3( 3), 371-382. (In Farsi)
  33. Sairam, R.K. & Srivastava. G.C. (2001). Water stress tolerance of wheat (Triticum aestivum ) variation in hydrogen peroxide accumulation and antioxidant activity in tolerant and susceptible genotype. Journal Agronomy and Crop Science, 186, 63-70.
  34. Valizadeh, J. & Valizadeh, M. (2009). In vitro callus induction and plant regeneration from Withania coagulans: a valuable medicinal plant. Pakistan Journal of Biological Sciences, 12(2), 1415-1419.
  35. Wisniewski, M., Carole, B. & Gusta, L.V. (2003). An overview of cold hardiness in woody plants: seeing the forest through the trees. Horticultural Science, 38, 952-
علوم باغبانی ایران
دوره 53، شماره 2
شهریور 1401
صفحه 341-350

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سابقه مقاله

  • تاریخ دریافت: 10 بهمن 1397
  • تاریخ بازنگری: 21 اردیبهشت 1398
  • تاریخ پذیرش: 30 اردیبهشت 1398

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