اثر باکتری‌های محرک رشد در مقاومت به تنش شوری گیاه کلم زینتی رقم کاموم ‏(‏Brassica oleraceae L.‎‏)‏

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشیار، دانشکده کشاورزی، دانشگاه زنجان، زنجان، ایران. ‏ کد پستی: 45371-38791‏

2 دانشجوی دکتری، دانشکده کشاورزی، دانشگاه زنجان، زنجان، ایران. ‏ کد پستی: 45371-38791‏

3 کارشناسی ارشد، دانشکده کشاورزی، دانشگاه زنجان، زنجان، ایران. ‏کد پستی: 45371-38791‏

4 استادیار، دانشکده کشاورزی، دانشگاه زنجان، زنجان، ایران. ‏کد پستی: 45371-38791‏

چکیده

به منظور مطالعه تأثیر باکتری‌ها بر خصوصیات مورفولوژی و بیوشیمی کلم زینتی تحت تنش شوری، آزمایشی به صورت فاکتوریل در قالب طرح کاملاً تصادفی در سه تکرار اجرا گردید. تیمارها شامل شوری در سه سطح (4 و 8 دسی زیمنس بر متر و شاهد (عدم شوری) و باکتری‌های محرک رشد در سه سطح (عدم تلقیح، Pseudomonas putida و Bacillus subtilis) بودند. صفات رویشی گیاه شامل ارتفاع بوته، تعداد برگ، وزن تر و خشک برگ، وزن تر و خشک بوته و ریشه، با افزایش میزان تنش شوری، کاهش معنی‌داری نسبت به تیمار شاهد نشان دادند و تلقیح با باکتری‌های محرک رشد به‌خصوص سودوموناس پوتیدا، موجب بهبود این صفات گردید. فعالیت آنتی‌اکسیدانی و فنل کل با افزایش شوری، افزایش یافت، به طوری که بیشترین میزان فنل و آنتی­اکسیدان در تیمار 8 دسی زیمنس بر متر (به ترتیب  mg/g FW 08/1 و  μmol/g FW52/62) مشاهده شد. از طرف دیگر استفاده از باکتری‌ باسیلوس توانست میزان فنل کل و آنتی­اکسیدان را در تقابل با شوری افزایش دهد (mg/g FW08/1 و μmol/g FW78/62). میزان پرولین برگ نیز، از یک روند افزایشی در سطوح مختلف تنش شوری برخوردار بود و بیش­ترین میزان آن در بالاترین سطح تنش شوری و کاربرد باکتری باسیلوس سوبتیلیس مشاهده گردید. اثر متقابل باکتری و تنش شوری، تجمع عنصر سدیم را کاهش و موجب افزایش میزان عنصر پتاسیم گردید. نتایج این آزمایش نشان داد باکتری‌های محرک رشد، به‌خصوص باکتری سودوموناس پوتیدا موجب کاهش خسارت ناشی از تنش شوری در گیاه کلم زینتی می‌شوند.

کلیدواژه‌ها

عنوان مقاله [English]

Effects of plant growth promoting rhizobacteria on salinity tolerance of ornamental ‎cabbage (Brassica oleraceae L. cv. Kamome)‎

نویسندگان [English]

  • Mitra Aelaei 1
  • Fahimeh Salehi 2
  • Morteza Bahrami 3
  • Mohsen Sanikhani 4
1 Associate Professor, Faculty of Agriculture, University of Zanjan, Zanjan, Iran, ‎Postal Code: 45371-38791.‎
2 Ph. D. Student,, Faculty of Agriculture, University of Zanjan, Zanjan, Iran, Postal Code: 45371-‎‎38791.‎
3 M. Sc., Faculty of Agriculture, University of Zanjan, Zanjan, Iran, Postal Code: 45371-38791‎
4 Assistant Professor, Faculty of Agriculture, University of Zanjan, Zanjan, Iran, Postal Code: 45371-38791.‎
چکیده [English]

In order to study the morphologies and biochemistry of ornamental cabbage under salt stress, a factorial experiment was carried out based on completely randomized design in three replications. The treatments included salinity at three levels (4 and 8 dS.m-1 and control (no salinity) and growth stimulating bacteria at three levels (no inoculation Pseudomonas putida and Bacillus subtilis). Plant growth characteristics, including plant height, number of leaves, fresh and dry weight of leaves, fresh and dry weight of plant and root, showed a significant decrease compared to the control treatment with increasing salinity stress, and inoculation with growth-promoting bacteria, especially Pseudomonas putida, improved these attributes. Antioxidant activity and total phenol increased with increasing salinity, so that the highest amount of phenol and antioxidant was observed at treatment 8 dS/m (1.08 mg/g FW and 62.52 μmol/g FW, respectively). On the other hand, the use of Bacillus bacteria could increase the amount of total phenol and antioxidants in contrast with salinity (1.08 mg/g FW and 62.78 μmol/g FW). The amount of proline in leaves also had an increasing trend at different levels of salinity stress, and its highest level was observed at the highest level of salinity stress and the application of Bacillus subtilis bacteria. The interaction between bacteria and salinity stress reduced the accumulation of sodium element and increased the amount of potassium element. The results of this experiment showed that growth-promoting bacteria, especially Pseudomonas putida bacteria, reduce the damage caused by salt stress in ornamental cabbage plants.

کلیدواژه‌ها [English]

  • Bacillus subtilis
  • proline
  • Pseudomonas putida
  • salinity stress‎

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علوم باغبانی ایران
دوره 53، شماره 2
شهریور 1401
صفحه 423-438

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  • تاریخ دریافت: 03 اردیبهشت 1400
  • تاریخ بازنگری: 31 تیر 1400
  • تاریخ پذیرش: 15 شهریور 1400

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