ارزیابی برخی ویژگی‌ها در لاین‌های جهش‌یافته نسل چهارم خرفه (‏Portulaca oleracea L.‎‏) حاصل ‏از تیمار با دی متیل سولفات ‏

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانش‌آموخته کارشناسی ارشد، دانشکده کشاورزی، دانشگاه شاهد، تهران، ایران‏

2 دانشیار، دانشکده کشاورزی، دانشگاه شاهد، تهران، ایران ‏

چکیده

القای جهش ازجمله روش­های مهم ایجاد تنوع ژنتیکی است. خرفه به دلیل محتوای بالای آنتی‌اکسیدان‌های مفید و اسیدهای چرب امگا-۳، مصارف داروئی منحصربه‌فردی دارد. در این تحقیق، تنوع ژنتیکی 24 لاین جهش­یافته نسل چهارم (M4) خرفه حاصل از تیمار با دی متیل سولفات به همراه شاهد در قالب طرح بلوک­های کامل تصادفی با سه تکرار بررسی شد. تفاوت بین لاین­ها ازنظر ارتفاع گیاه، طول برگ، عرض برگ، شکل برگ، وزن خشک ساقه، و شاخص سبزینگی برگ در گیاه 30 روزه معنی­دار بود. تجزیه مختصات اصلی و تجزیه خوشه­ای، لاین­ها را در دو خوشه مجزا گروه­بندی کرد. لاین­های خوشه یک ازنظر بیشتر صفات مانند وزن تر ساقه و برگ و تعداد کپسول نسبت به لاین­های خوشه دو برتری معنی‌دار داشتند. صفت تعداد برگ با وزن ‌تر ساقه، وزن‌ تر برگ، وزن‌ تر گیاه، تعداد کپسول و نسبت وزن خشک به وزن‌تر گیاه به‌طور معنی­دار همبستگی داشت. در تجزیه علیت مراتبی، وزن ‌تر ساقه در مرتبه نخست تنها صفت مؤثر بر تعداد کپسول بود و در مرتبه دوم، وزن ‌تر برگ و ارتفاع گیاه مهم‌ترین صفاتی بودند که از طریق اثر مستقیم بر وزن‌ تر ساقه، بر روی تعداد کپسول تأثیر داشتند. لاین جهش یافته L18 از نظر برخی صفات مهم زراعی مانند ارتفاع گیاه، طول برگ و تعداد کپسول در بوته نسبت به شاهد برتری معنی‌دار داشت که می‌توان از آن برای تحقیقات آتی به­خصوص برای اصلاح گیاه خرفه باهدف تجاری‌سازی و کاشت وسیع برای مصارف ازجمله سبزی، استحصال روغن، و همچنین کاربردهای داروئی استفاده نمود.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Evaluation of some characteristics of the fourth generation of common purslane ‎‎(Portulaca oleracea L.) mutated from treatment with dimethyl sulfate ‎

نویسندگان [English]

  • Maryam Nasrollahi Pari 1
  • Mohammad Hossein Fotokian 2
1 Graduated M. Sc. Student , Faculty of Agriculture, Shahed University, Tehran, Iran
2 Associate Professor, Faculty of Agriculture, Shahed University, Tehran, Iran
چکیده [English]

Mutation induction is an important method for generating genetic diversity. Purslane (Portulaca oleracea L.) has unique medicinal uses due to its high content of beneficial antioxidants and omega-3 fatty acids. In this study, the genetic diversity of 24 mutant lines of fourth-generation (M4) purslane obtained from dimethyl sulfate treatment together with the control was investigated in a randomized complete block design with 3 replicates. The differences among the lines were significant in traits including plant height, leaf length, leaf width, stem dry weight, and leaf chlorophyll in the 30-day-old plant. The principal coordinates and cluster analyses divided the lines into two separate clusters. The lines in cluster 1 had a significant advantage over the lines in cluster 2 for most traits such as stem fresh weight, leaf fresh weight, and number of capsules per plant. The number of leaves was significantly correlated with stem fresh weight, leaf fresh weight, plant fresh weight, number of capsules per plant and the ratio of plant dry weight to plant fresh weight. In sequential path analysis, stem fresh weight in the first order was the only trait affecting number of capsules per plant, and in the second-order, leaf fresh weight and plant height were the most important traits that had an effect on number of capsules per plant through the direct effect of stem fresh weight. L18 mutant line had a significant advantage over the control in some agronomic traits such as plant height, leaf length and number of capsules per plant, that can be used for future research, especially for cultivation, commercialization and extensive planting for uses as vegetables, oil extraction, and medicinal applications.

کلیدواژه‌ها [English]

  • Mutation induction
  • principal coordinate analysis
  • sequential path analysis
  • variance components

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علوم باغبانی ایران
دوره 53، شماره 4
دی 1401
صفحه 881-890

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سابقه مقاله

  • تاریخ دریافت: 14 آبان 1400
  • تاریخ بازنگری: 17 آذر 1400
  • تاریخ پذیرش: 19 خرداد 1401

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