بررسی تغییرات غلظت عناصر غذایی در برگ مکزیکن لایم (‏Citrus aurantifolia Swingle‏) طی ‏پیشرفت بیماری جاروک

نوع مقاله : مقاله پژوهشی


1 استادیار، پژوهشکده مرکبات و میوه‌های نیمه‌گرمسیری، مؤسسه تحقیقات علوم باغبانی، سازمان تحقیقات، آموزش و ترویج ‏کشاورزی، رامسر، ایران

2 دانشیار، پژوهشکده مرکبات و میوه‌های نیمه‌گرمسیری، مؤسسه تحقیقات علوم باغبانی، سازمان تحقیقات، آموزش و ترویج ‏کشاورزی، رامسر، ایران

3 استادیار، مرکز تحقیقات کشاورزی و منابع طبیعی مازندران، مازندران، سازمان تحقیقات، آموزش و ترویج کشاورزی، ساری، ‏ایران


بیماری جاروک مکزیکن لایم، که توسط Candidatus Phytoplasma aurantifolia ایجاد می‌شود، مهم‌ترین عامل محدودکننده تولید این محصول در مناطق جنوبی ایران است. این پژوهش با هدف بررسی تغییرات غلظت عناصر غذایی در برگ نهال‌های ریشه‌دار مکزیکن‌لایم آلوده‌شده با فیتوپلاسما و مکزیکن‌لایم سالم طی پیشرفت بیماری (330-90 روز) و امکان جداسازی نهال‌های سالم و آلوده تحت شرایط گلخانه‌ای انجام گرفت. بدین‏منظور، تعدادی برگ از نهال‏های آلوده و سالم در بازه‌های زمانی 90، 150، 210، 270 و 330 روز پس از آلوده‏سازی نمونه‌برداری شد و غلظت عناصر غذایی شامل نیتروژن، فسفر، پتاسیم، کلسیم، منیزیم، بور، آهن، منگنز، روی و مس طی پیشرفت بیماری در برگ گیاهان اندازه‏‏گیری شد. نتایج نشان داد آلودگی به فیتوپلاسما منجر به کاهش معنی‏دار غلظت نیتروژن، کلسیم، بور و منگنز و افزایش معنی­دار غلظت فسفر، پتاسیم، منیزیم، آهن و روی در برگ گیاهان آلوده شد. غلظت مس در برگ گیاهان آلوده در مقایسه با گیاهان سالم تغییر معنی‏داری نداشت. به علاوه، نتایج تجزیه به مولفه‌های اصلی نشان داد بر اساس غلظت عناصر غذایی نمی­توان گیاهان آلوده و سالم را در مراحل اولیه نمونه‏‏برداری (قبل از ظهور علایم ظاهری بیماری) جداسازی نمود، اما با ظهور علائم ظاهری بیماری و پیشرفت بیماری، آنالیز مولفه اصلی قادر به جداسازی گیاهان آلوده از گیاهان سالم بود. به‏طور کلی نتایج مطالعه حاضر بینشی جدید در مورد پاسخ مکزیکن‌لایم به بیماری‏گر فیتوپلاسما فراهم نمود و می­تواند در اتخاذ راهکارهایی در جهت بازتوانی و افزایش عمر اقتصادی درختان آلوده به جاروک مورد استفاده قرار گیرد.


عنوان مقاله [English]

Evaluation of leaf nutrient changes in Mexican lime (Citrus aurantifolia Swingle) ‎during the progression of witches’ broom disease of lime (WBDL)‎

نویسندگان [English]

  • Tahereh Raiesi 1
  • Morteza Golmohammadi 2
  • Ali Asadi kangarshahi 3
1 Assistant Professor, Citrus and Subtropical Fruit Research Centre, Horticultural Science Research Institute, Agricultural Research, ‎Education and Extension Organization (AREEO), Ramsar, Iran
2 Associate Professor, Citrus and Subtropical Fruit Research Centre, Horticultural Science Research Institute, Agricultural Research, ‎Education and Extension Organization (AREEO), Ramsar, Iran
3 Assistant Professor, Mazandaran Agricultural and Natural Resources Research Center, Agricultural Research, Education and ‎Extension Organization (AREEO), Sari, Iran
چکیده [English]

Witches’ broom disease of lime (WBDL), caused by ‘Candidatus Phytoplasma aurantifolia’, is major limiting factor for Mexican lime (Citrus aurantifolia Swingle) production in southern Iran. In this investigation, leaf nutrient profiling of the healthy and infected rooted Mexican lime during WBDL progression (90-330 days) as well as to distinguish the infected from the healthy Mexican limes under greenhouse condition. For this aim, the healthy and infected plants were sampled 90, 150, 210, 270 and 330 days after inoculation with phytoplasma and leaf nutrient concentrations including nitrogen, phosphorus, potassium, calcium, magnesium, boron, iron, manganese, zinc and copper were measured during WBDL progression. The results showed phytoplasmal infection mostly caused to a marked decrease in the concentration of nitrogen, calcium, boron, manganese, and significant increase in phosphorus, magnesium, potassium, iron, and zinc in the infected leaves. In Mexican lime leaves, copper concentration did not significantly change after inoculation with phytoplasma with respect to the healthy plants. In addition, the principal components analysis (PCA) based on the concentration of nutrients could not distinction infected and healthy plants in the early stages of sampling (before the appearance of symptoms), but PCA revealed a clear distinction between the leaf nutritional profiles of healthy and infected plants after the appearance of symptoms during the progression of WBDL. In conclusion, this study provides new insights into the lime response to phytoplasma infection during the progression of WBDL and it can be useful in adopting strategies to improve and increase the economic efficiency of trees infected with WBDL.

کلیدواژه‌ها [English]

  • Macro nutrient
  • micro nutrient
  • PCA
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