نوع مقاله : مقاله پژوهشی
نویسندگان
1 دانشجوی دکتری، گروه علوم باغبانی، دانشکده علوم کشاورزی، دانشگاه گیلان، رشت، ایران
2 استادیار، گروه علوم باغبانی، دانشکده علوم کشاورزی، دانشگاه گیلان، رشت، ایران
3 دانشیار، گروه علوم باغبانی، دانشکده علوم کشاورزی، دانشگاه گیلان، رشت، ایران
چکیده
ارکیدهها از معروفترین گیاهان زینتی در جهان هستند. از مهمترین چالشهای تولید ارکیدهها، دشواری ازدیاد آنها است از این رو ریزازدیادی به عنوان روش تکثیر آن کاربرد دارد. در این مطالعه ریزازدیادی تجاری گیاه ارکیده Phalaenopsis amabilis cv. Detroit با استفاده از اندام‌های رویشی انجام شد. ابتدا گرههای روی ساقه گل، جهت باززایی مستقیم در محیط کشت MS با غلظتهای متفاوتی از NAA و BAP کشت شدند و گیاهچههای حاصل جهت ریشهزایی در محیط کشت ½MSحاوی غلظتهای مختلفی از IAA و NAA و زغال فعال قرارداده شدند. برگهای استریل گیاهچهها نیز در محیط کشت ½MS دارای BAP،NAA و TDZ جهت بررسی قابلیت باززایی و تولید مستقیم جنین‌های بدنی کشت شدند. نتایج نشان داد موثرترین غلظت جهت باززایی گیاهچه در محیط کشت MS دارای ۱ میلیگرم در لیتر NAA و ۴ میلی گرم در لیتر BAP به دست آمد. بیشترین تعداد ریشه از تیمار 2 میلیگرم در لیتر NAA بهدست آمد. بیشترین پروتوکورم از تیمار 3 میلیگرم در لیتر TDZ به دست آمد. میزان زندهمانی گیاهچههای باززایی شده از ریزنمونه گرههای روی ساقه گل 93/84 درصد بود. در نهایت، بالاترین سازگاری گیاهچهها ) 2/90 درصد( در محیط حاوی کوکوپیت و زغال فعال با نسبت 1 به 1 بهدست آمد.
کلیدواژه‌ها
عنوان مقاله [English]
The study of large scale Phalaenopsis amabilis cv. Detroit plantlets production through in vitro culture of the vegetative organs
نویسندگان [English]
- Khosro Balilashaki 1
- Hedayat Zakizadeh 2
- Jamal-Ali Olfati 3
1 Ph. D. Candidate, Department of Horticultural Sciences, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran
2 Assistant Professor, Department of Horticultural Sciences, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran
3 Associate Professor, Department of Horticultural Sciences, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran
چکیده [English]
Orchids are one of the most popular plants in the world. Because of its hard propagation as most important problem, micropropagation technique has been employed recently. In this study, commercial micropropagation of orchid Phalaenopsis cut flower “Detroit” was done by vegetative tissue. First, nodal explants of Phalaenopsis amabilis cv. Detroit flower stalks cultured on Murashige and Skoog (MS) medium supplemented with different concentration of NAA and BAP for direct regeneration and the obtained planlets were cultured on ½ MS medium supplemented with different combination of NAA, IAA and activated charcoal for rooting. The sterile leaves of plantlets were cultured on ½ MS medium containing NAA, BAP and TDZ for evaluation of regeneration and direct production of somatic embryos. Results showed that effective concentration for plantlet regeneration obtained in MS medium containing 1 mg/l NAA and 4 mg/l BAP. The highest number of root produced in 2 mg/l NAA. The highest number of protocorms obtained at 3 mg/l TDZ. The plants survived rates from nodal flower stalk explants was 84.93%. At the end, the highest plantlets acclimatization (90.20 %) was in medium containing cocopeat and activated charcoal (1: 1).
کلیدواژه‌ها [English]
- Acclimatization
- plant growth regulators
- protocorm
- regeneration
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