تأثیر تلقیح ریشه‌ای قارچ مایکوریزا (‏Glomus mosseae‏) بر تحمل به خشکی نهال‌های لیلکی ایرانی ‏‏(‏Gleditsia caspica Desf‏)‏

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانش‌آموخته کارشناسی ارشد، دانشکده کشاورزی و منابع طبیعی، دانشگاه اراک، اراک، ایران

2 دانشیار، دانشکده کشاورزی و منابع طبیعی، دانشگاه اراک، اراک، ایران

چکیده

خشکی یکی از مهمترین عوامل محیطی محدودکننده کشت گیاهان چوبی می‌باشد. به‌منظور افزایش تحمل نهال‌های لیلکی ایرانی به تنش خشکی از طریق همزیستی با قارچ آربسکولار مایکوریزا، آزمایشی به‌صورت فاکتوریل در قالب طرح کاملاً تصادفی در سال‌های ۱۳۹۷-۱۳95 اجرا شد. فاکتورهای آزمایش شامل مایکوریزا در دو سطح (تلقیح یا عدم تلقیح با Glomus moseae) و تنش خشکی در دو سطح (آبیاری یا عدم آبیاری) بود. تنش خشکی باعث کاهش معنی‌دار صفات رشدی از جمله محتوای آب نسبی، درصد ماده خشک اندام‌های هوایی و ریشه، محتوای کلروفیل کل، محتوای کاروتنوئید کل و شاخص پایداری غشاء سلولی و افزایش معنی‌دار محتوای پرولین شد. تلقیح ریشه نهال‌ها با قارچ مایکوریزا باعث بهبود محتوای آب نسبی (به‌میزان 14 درصد)، محتوای کاروتنوئید کل (به‌میزان 25 درصد)، درصد ماده خشک اندام‌های هوایی (به‌میزان 5/4 درصد)، درصد ماده خشک ریشه (به‌میزان 2/6 درصد) و ظرفیت آنتی‌اکسیدانی کل (به‌میزان 8/54 درصد) نسبت به نهال‌های تلقیح‌نشده گردید. همچنین شاخص پایداری غشاء سلولی، محتوای کلروفیل کل، محتوای فنل کل و فلاونوئید کل در نهال‌های تلقیح شده نسبت به نهال‌های تلقیح نشده چه در شرایط تنش خشکی و چه در شرایط آبیاری نرمال بیشتر بود. به‌طورکلی، نتایج نشان داد قارچ مایکوریزا باعث بهبود ویژگی‌های رشدی و کاهش اثرات سوء تنش خشکی از طریق افزایش ترکیبات آنتی‌اکسیدانی در نهال‌های لیلکی ایرانی گردید.

کلیدواژه‌ها

عنوان مقاله [English]

Effect of root inoculation of mycorrhiza fungi (Glomus mosseae) on growth and ‎resistance to drought stress in Gleditsia caspica seedlings

نویسندگان [English]

  • Payam Puryafar 1
  • Alireza Khaleghi 2
  • Ahmadreza Abbasifar 2
  • Mina Taghizadeh 2
1 M. Sc.‎‏ ‏Graduate, Faculty of Agriculture and Natural Resources, Arak University, Arak, Iran
2 Associate Professor, Faculty of Agriculture and Natural Resources, Arak University, Arak, Iran
چکیده [English]

Drought is one of the most important environmental factors which limit the growth of woody plant. In order to evaluvate the resistance of Caspian locust seedlings to drought stress through inoculation with arbuscular mycorrhiza fungi a factorial experiment was performed in completely randomized design in 2016-2018. The factors were included mycorrhiza at two levels (inoculation and uninoculated with Glomus moseae) and drought stress at two levels (Well-watered and withholding water). Drought stress significantly reduced growth traits such as relative water content, percentage of aerial and root dry biomass, content of total chlorophyll, total carotenoid content and cell membrane stability index and significantly increased proline content. Root inoculation of seedlings with mycorrhizal fungi improved relative water content (14%), total carotenoid content (25%), aerial dry biomass percentage (4.5%), root dry biomass percentage (6.2%), and total antioxidant capacity (54.8%) than to uninoculated seedlings. Also, cell membrane stability index, total chlorophyll content, total phenol content and total flavonoid content in inoculated seedlings were higher than non-inoculated seedlings under both drought and normal irrigation conditions. Overall, the results showed that mycorrhizal fungi improved growth characteristics and reduced adverse effects of drought stress by increasing antioxidant compounds in Caspian locust seedlings.

کلیدواژه‌ها [English]

  • Arbuscular mycorrhiza
  • Caspian locust
  • drought stress

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علوم باغبانی ایران
دوره 52، شماره 3
آذر 1400
صفحه 633-645

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سابقه مقاله

  • تاریخ دریافت: 02 بهمن 1398
  • تاریخ بازنگری: 15 خرداد 1400
  • تاریخ پذیرش: 15 تیر 1400

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