مطالعه اثر اسپرمین در شرایط تنش شوری بر خصوصیات مورفوفیزیولوژیکی گیاه پروانش ‏‏(‏Chatarantus roseus L.‎‏)‏

نوع مقاله : مقاله پژوهشی

نویسندگان

1 استادیار، دانشکده کشاورزی، دانشگاه‎ ‎زنجان،‎ ‎زنجان،‎ ‎ایران. ‏45371-38791‏

2 دانشجوی کارشناسی ارشد، دانشکده کشاورزی، دانشگاه‎ ‎زنجان،‎ ‎زنجان،‎ ‎ایران. ‏45371-38791‏

3 دانشجوی دکتری، دانشکده کشاورزی، دانشگاه‎ ‎زنجان،‎ ‎زنجان،‎ ‎ایران. ‏45371-38791‏

چکیده

به منظور مطالعه اثر اسپرمین در شرایط تنش شوری در گیاه پروانش آزمایشی به صورت فاکتوریل در قالب طرح کاملاً تصادفی در گلدان­های که حاوی پیت ماس قهوه­ای به پرلایت با نسبت 4:1 بودند در گلخانه دانشگاه زنجان اجرا شد. تیمارها شامل اسپرمین  در چهار سطح (صفر، 5، 10 و 15 ppm) و تنش شوری در سه سطح (صفر، 60 و 120 میلی­مولار کلریدسدیم) بودند. صفات مورد ارزیابی شامل صفات مورفولوژیکی(وزن تر، وزن خشک، ارتفاع، قطر گل، تعداد برگ)، صفات فیزیولوژی(محتوای نسبی آب، کلروفیل کل، کاروتنوئید) و فعالیت آنزیم­های آنتی‌اکسیدانی پراکسیداز و مقدار پرولین بودند. نتایج نشان داد کاربرد پلی­آمین اسپرمین رشد و عملکرد گیاه پروانش را در شرایط تنش بهبود بخشید و بر صفات وزن تر و وزن خشک بوته، ارتفاع، تعداد گل، محتوای نسبی آب، پرولین، محتوای کلروفیل، کاروتنوئید و آنزیم پراکسیداز در سطح احتمال یک درصد و بر صفت تعداد برگ در سطح احتمال پنج درصد اثر معنی‌داری نشان داد. بیشترین میزان پرولین (37/0µmol/g FW) در تیمار 10پی‌پی‌ام و کمترین میزان (23/0 µmol/g FW) در تیمار شاهد بدست آمد. بالاترین میزان آنزیم پراکسیداز (099/0 units.g-1 FW.min-1) در تیمار 15 پی‌پی‌ام اسپرمین به دست آمد که نشان­دهنده افزایش میزان فعالیت این آنزیم نسبت به شاهد با افزایش سطح اسپرمین بود. بنابراین استفاده از اسپرمین در زمینه دستیابی به شاخص­های رشد مطلوب در این گیاه سودمند است.

کلیدواژه‌ها


عنوان مقاله [English]

The Study of effects of spermine under salinity stress on morphophysiological ‎characteristics of Catharanthus roseus L.‎

نویسندگان [English]

  • Mitra Aelaei 1
  • Zahra Karami 2
  • Masood Arghavani 1
  • Fahimeh Salehi 3
1 Assistant Professor, Faculty of Agriculture, University of Zanjan, Zanjan, Iran,‎ ‎45371-38791‎
2 M.Sc. Student, Faculty of Agriculture, University of Zanjan, Zanjan, Iran,‎ ‎45371-38791‎
3 Ph.D. Candidate, Faculty of Agriculture, University of Zanjan, Zanjan, Iran,‎ 45371-38791‎
چکیده [English]

In order to study the effects of spermine in Catharanthus roseus L under salinity stress conditions, a factorial experiment was conducted in a completely randomized design pots containing 4:1 brown peat moss to perlite in greenhouse of Zanjan University. Treatments were four spermine at four levels (0, 5, 10 and 15 ppm) and salinity stresses at three levels (0, 60 and 120 mM) of sodium chloride. Traits which were studied were as fresh and dry weight, plant height, flower diameter, leaf number as morphological traits, relative water content, total chlorophyll, carotenoids as physiological traits, activity of antioxidant enzymes such as peroxidase and proline. Results showed that application of polyamine spermine improves growth and yield of Chatarantus roseus L in stress condition and on fresh and dry weight, plant height, flower number, relative water content, proline, chlorophyll content, carotenoid, peroxidase enzyme and leaf number at 1% and 5% probability levels. The highest proline content (0.37 µmol/g FW) was obtained in 10 ppm and the lowest (0.23 µmol/g FW) in control treatment. The highest peroxidase enzyme (0.099 units. g-1 FW.min-1) was obtained in 15 ppm spermine treatment which showed an increase in spermine compared to the control. Therefore, the use of spermine in this plant for achieving desirable growth indices is recommended and can be beneficial.

کلیدواژه‌ها [English]

  • Carotenoid
  • growth indices
  • Peroxidase
  • proline‎
  1. Alborz, Z., Habibi, F. & Mortazavi, S. (2014). Effect of spraying the putrescine and spermine on vase life of Alestromeria cv. “Sukari”. Journal of Agricultural Crop Management, 17 (1), 241-255. (in Farsi).
  2. Alcazar, R., Marco, F., Cuevas, J. C., Patron, M., Ferrando, A., Carrasco, P., Tiburcio, A. F. & Altabella, T. (2006). Involvement of polyamines in plant response to abiotic stress. Biotechnology Letters, 28, 1867-
  3. Arnon, D.I. (1949). Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiology, 24 (1), 1-3.
  4. Baniasadi, F., Safari, V.R. & Maghsoudi moud, A.A. (2014). Effect of spermidine and spermine on some growth parameters (Calendula officinalis). In: Proceeding of 1th National Congress on Flowers and Ornamental Plants, 21-24 Oct., National Institute of Flowers and Ornamental Plants, Karaj, Iran, 6, 21-23. (in Farsi).
  5. Barker, D., Sullivan, C. & Moser, L. E. (1993). Water deficit effects on osmotic potential, cell wall elasticity, and proline in five forage grasses. Agronomy Journal, 85 (2), 270-275.
  6. Barres, H.D. & Weatherley, P.E. (1962). A re-examination of the relative turgidity techniques for sstimating water deficits in leaves. Australian Journal of Biological Sciences, 15, 413-428.
  7. Bates, L.S., Waldren, R.P. & Teare, I.D. (1973). Rapid determination of free proline for water-stress studies. Plant and Soil, 39(1), 205-207.
  8. Castilla, N. & Lopez-Galvez, J. (1994). Vegetable crop responses in improved low-cost plastic greenhouses. Journal of Horticultural Science, 69(5), 915-921
  9. Dat, J., Vandenabeele, S., Vranová, E., Van Montagu, M., Inzé, D. & Van Breusegem, F. (2000). Dual action of the active oxygen species during plant stress responses. Cellular and Molecular Life Sciences, 57 (5), 779-795.
  10. Das, C., Sengupta, T., Chattopadhyay, S., Setua, M., Das, N.K. & Saratchandra, B. (2002). Involvement of kinetin and spermidine in controlling salinity stress in mulberry (Morus alba L. Cv. S 1). Acta Physiologiae Plantarum, 24(1), 53-57.
  11. Dhindsa, R.S., Plumb-Dhindsa, P. & Thorpe, T. A. (1981). Leaf senescence: correlated with increased levels of membrane permeability and peroxidation, and decreased levels of superoxide dismutase and catalase. Journal of Experimental Botany, 32(1), 93-101.
  12. Emraei tabar, S., Ershadi, A. & Robati, T. (2016). The effect of putrescine and spermine on almond and peach drought tolerance. Journal of Agricultural Crop Management, 18 (1), 203-218. (in Farsi).
  13. Engel, N., Schmidt, M., LÜTz, C. & Feierabend, J. (2006). Molecular identification, heterologous expression and properties of light‐insensitive plant catalases. Plant, Cell & Environment, 29 (4), 593-607.
  14. Farjadi shakib, M., Naderi, R. & Mashhadi, M. (2013). The effect of spermidine foliar application on morphological, physiological and biochemical properties of Iranian cyclamen. Journal of Plant Ecophysiology, 31 (2), 21-23. (In Farsi).
  15. Farooq, M., Wahid, A. & Lee, D. J. (2009). Exogenously applied polyamines increase drought tolerance of rice by improving leaf water status, photosynthesis and membrane properties. Acta Physiologiae Plantarum, 31 (5), 937-945.
  16. Ghahsare, M. & Kafi, M. (2014). Scientific and practical flowering. Vol 1. Esfahan Publications. (In Farsi).
  17. Gibon, Y., Sulpice, R. & Larher, F. (2000). Proline accumulation in canola leaf discs subjected to osmotic stress is related to the loss of chlorophylls and to the decrease of mitochondrial activity. Physiologia Plantarum, 110 (4), 469-476.
  18. Groppa, M.D. & Benavides, M.P. (2008). Polyamines and abiotic stress: recent advances. Amino acids, 34 (1), 35-36.
  19. Gunes, A., Inal, A. & Alpaslan, M. (1996). Effect of salinity on stomatal resistance, proline, and mineral composition of pepper. Journal of Plant Nutrition, 19 (2): 389-396.
  20. Homai, M. (2002). Plant response to salinity. Publication of the Scientific Committee on Irrigation and Drainage. No. 1. (In Farsi).
  21. Jalili marandi, R. (2010) Physiology of environmental stresses and mechanisms of resistance in horticultural plants (Fruit trees, vegetables, ornamental plants and medicinal plants). First volume. University Jihad Publications, Urmia. (In Farsi).
  22. Kamyab, F. (2016). Effect of different polyamines on vase life, ethylene production and some physiological traits of Dianthus caryopyllus Cv. Red Corsa. Iranian Journal of Agronomy, 18 (2), 275-288. (in Farsi).
  23. Kalidass, C., Ramasamy Mohan, V. & Daniel, A. (2010). Effect of auxin and cytokinin on vincristine production by callus cultures of Catharanthus roseus(apocynaceae). Tropical and Subtropical Agroecosystems, 12 (2), 10-13.
  24. Koca, H., Ozdemir, F. & Turkan, I. (2006). Effect of salt stress on lipid peroxidation and superoxide dismutase and peroxidase activities of Lycopersicon esculentum and pennellii. Biologia Plantarum, 50 (4), 745-748.
  25. Kováčik, J., Bačkor, M. & Kadukova, J. (2008). Physiological responses of Matricaria chamomilla to cadmium and copper excess. Environmental Toxicology, 23 (1), 123-130.
  26. Kuznetsov, V.V. & Shevyakova, N. (1999). Proline under stress: biological role, metabolism, and regulation. Russian Journal of Plant Physiology, 46(2), 274-287.
  27. Lee, M.M., Lee, S.H. & Park, K.Y. (1997). Effects of spermine on ethylene biosynthesis in cut carnation (Dianthus caryophyllus) flowers during senescence. Journal of Plant Physiology, 151, 68-73.
  28. Lester, G.E. (2000). Polyamines and their cellular anti-senescence properties in honey dew muskmelon fruit. Plant Science, 160, 105-112.
  29. Liu, J.H., Kitashiba, H., Wang, J., Ban, Y. & Moriguchi, T. (2007). Polyamines and their ability to provide environmental stress tolerance to plants. Plant Biotechnology, 24, 117-126.
  30. Liu, K., Fu, H., Bei, Q. & Luan, S. (2000). Inward potassium channel in guard cells as a target for polyamine regulation of stomatal movements. Plant Physiology, 124(3), 1315-1326.
  31. Liu, J., Tong, L.P., Shen, T.W., Li, J., Wu, L. & Yu, Z.L. (2006). Impact of ion implantation on licorice (Glycyrrhiza uralensis Fisch) growth and antioxidant activity under drought stress. Plasma Science and Technology, 9 (3), 301-306.
  32. Mahgoub, M.H., El Aziz, N.A. & Mazhar, M.A. (2011). Response of Dahlia pinnata plant to foliar spray with putrescine and thiamine on growth, flowering and photosynthetic pigments. American-Eurasian Journal of Agricultural & Environmental Sciences, 10(5), 769-775.
  33. Miraabolghasemi, M., Aelaei, M., Kheiry, A. & Ghahramani, Z. (2021). Effect of γ-aminobutyric acid and spermine on morphophysiological traits and pigmentation of Rosa damascena Iranian Journal of Horticultural Science. 52 (1), 47-60. (in Farsi).
  34. Moradi, F. (2002). Physiological characterization of rice cultivars for salinity tolerance during vegetative and reproductive stages. PhD Thesis. The University of Philippines, Philippines.
  35. Mortazavi, S.H., Arghavani, M., Hassanpour Asil, M. & Kheiri, A. (2021). Effect of polyamines on morphophysiological characteristics of Dutch iris (Iris hollandica 'Blue Magic') cut flower. Iranian Journal of Horticultural Science. 52 (2), 269-280. (in Farsi).
  36. Nahed, A.A, Taha Lobna, G. & Ibrahim Soad, S. (2009). Some studies on the effect of putrescine, ascorbic acid and thiamine on growth, flowering and some chemical constituents of Gladiolus plants at Nubaria. Ozean Journal of Apple Science, 2(2), 12-23.
  37. Omid baigi, R. (2007). Production and processing approaches for medicinal plants. Astan Ghods Razavi Publications. (In Farsi).
  38. Orabi, S.A., Salman, S.R. & Shalaby, M.A. (2010). Increasing resistance to oxidative damage in cucumber (Cucumis sativus) plants by exogenous application of salicylic acid and paclobutrazol. World Journal of Agricultural Sciences, 6 (3), 252-259.
  39. Papenfus, H., Kulkarni, M., Stirk, W., Finnie, J. & Van Staden, J. (2013). Effect of a commercial seaweed extract (Kelpak®) and polyamines on nutrient-deprived (N, P and K) okra seedlings. Scientia Horticulturae, 151, 142-146.
  40. Ritchie, S. W., Nguyen, H. T. and Holaday, A. S. (1990). Leaf water content and gas exchange parameters of two wheat genotypes differing in drought resistance, Crop Science, 30, 105-111.
  41. Roychoudhury, A., Basu, S. & Sengupta, D.N. (2011). Amelioration of salinity stress by exogenously applied spermidine or spermine in three varieties of indica rice differing in their level of salt tolerance. Journal of Plant Physiology, 168 (4), 317-328.
  42. Sankar, B.E., Jaleel, C.A., Manivannan, P., Kishorekumar, A., Somasundaram, R. & Panneerselvam, R. (2007). Drought-induced biochemical modifications and proline metabolism in (Abelmoschus esculentus) Moench. Acta Botanica Croatica, 66 (1), 43-56.
  43. Santiago, M., Diego, H., Sauchez, A. G., Alponso, V. & Oscar A. R. (2004). Effect of polyamines on growth and salinity resistance of two rice cultivars. Biology Planta, 54, 199-201.
  44. Saxena, N. P., Krishnamurthy, L. & Johansen, C. (1993). Registration of a drought-resistant chickpea germplasm. Crop Science, 33(6), 1424-1424.
  45. Schonfeld, M.A., Johnson, R.C., Carver, B.F. & Mornhinweg, D.W. (1988). Water relations in winter wheat as drought resistance indicators. Crop Science, 28(3), 526-531.
  46. Shao, H.B., Chu, L.Y., Jaleel, C.A. & Zhao, C.X. (2008). Water-deficit stress induced anatomical changes in higher plants. Comptes Rendus Biologies, 331, 215-225.
  47. Sharma, S. & Uppal, K.S.K. (1999). Influence of salt stress on growth and quality on sugarcane. Indian Sugar, 22, 1-4.
  48. Sinclair, T. & Ludlow, M. (1985). Who taught plants thermodynamics? The unfulfilled potential of plant water potential. Functional Plant Biology, 12(3), 213-217.
  49. 49 Singh, A. & Prasad, R. (2009). Salt stress effects growth and cell wall bound enzymes in Arachis hypogaea seedlings. International Journal of Integrative Biology, 7 (2), 117-123.
  50. Tang, W. & Newton, J.R. (2005). Polyamines reduced salt induced oxidative damage by increasing the activities of antioxidant enzymes and decreasing lipid peroxidation in Virginia pine. Plant Growth Regulation, 46, 31-43.
  51. Turhan, E. & Eris A. (2004), Effects of sodium chloride applications and different growth media on ionic composition in strawberry plant. Journal Plant Nutrition, 27, 1653- 1662.
  52. Younesnia omran, F. (2015). The role of polyamines in salt stress tolerance under in-vitro condition in Lilium ledebourii M.Sc. Thesis, Mohaghegh Ardebili University, Ardebil, Iran. (in Farsi)
  53. Zadnour, P., Khosh-Khui, M. & Rahimian, A. (2011). Effects of putrescine, spermine and spermidine on flowering and vegetative growth of gladiolus plants. In: Proceeding of 7th Congress on Iranian Horticultural Science. 5-8 Sep., Isfehan University of Technology, Isfehan, Iran, 7, 651-654. (in Farsi).
  54. Zargari, A. (1997). Medicinal plants. University of Tehran Publications, Vol 3, Tehran. (in Farsi).