مطالعه اثر اسپرمین در شرایط تنش شوری بر خصوصیات مورفوفیزیولوژیکی گیاه پروانش ‏‏(‏Chatarantus roseus L.‎‏)‏

نوع مقاله : مقاله پژوهشی


1 استادیار، دانشکده کشاورزی، دانشگاه‎ ‎زنجان،‎ ‎زنجان،‎ ‎ایران. ‏45371-38791‏

2 دانشجوی کارشناسی ارشد، دانشکده کشاورزی، دانشگاه‎ ‎زنجان،‎ ‎زنجان،‎ ‎ایران. ‏45371-38791‏

3 دانشجوی دکتری، دانشکده کشاورزی، دانشگاه‎ ‎زنجان،‎ ‎زنجان،‎ ‎ایران. ‏45371-38791‏


به منظور مطالعه اثر اسپرمین در شرایط تنش شوری در گیاه پروانش آزمایشی به صورت فاکتوریل در قالب طرح کاملاً تصادفی در گلدان­های که حاوی پیت ماس قهوه­ای به پرلایت با نسبت 4:1 بودند در گلخانه دانشگاه زنجان اجرا شد. تیمارها شامل اسپرمین  در چهار سطح (صفر، 5، 10 و 15 ppm) و تنش شوری در سه سطح (صفر، 60 و 120 میلی­مولار کلریدسدیم) بودند. صفات مورد ارزیابی شامل صفات مورفولوژیکی(وزن تر، وزن خشک، ارتفاع، قطر گل، تعداد برگ)، صفات فیزیولوژی(محتوای نسبی آب، کلروفیل کل، کاروتنوئید) و فعالیت آنزیم­های آنتی‌اکسیدانی پراکسیداز و مقدار پرولین بودند. نتایج نشان داد کاربرد پلی­آمین اسپرمین رشد و عملکرد گیاه پروانش را در شرایط تنش بهبود بخشید و بر صفات وزن تر و وزن خشک بوته، ارتفاع، تعداد گل، محتوای نسبی آب، پرولین، محتوای کلروفیل، کاروتنوئید و آنزیم پراکسیداز در سطح احتمال یک درصد و بر صفت تعداد برگ در سطح احتمال پنج درصد اثر معنی‌داری نشان داد. بیشترین میزان پرولین (37/0µmol/g FW) در تیمار 10پی‌پی‌ام و کمترین میزان (23/0 µmol/g FW) در تیمار شاهد بدست آمد. بالاترین میزان آنزیم پراکسیداز (099/0 units.g-1 FW.min-1) در تیمار 15 پی‌پی‌ام اسپرمین به دست آمد که نشان­دهنده افزایش میزان فعالیت این آنزیم نسبت به شاهد با افزایش سطح اسپرمین بود. بنابراین استفاده از اسپرمین در زمینه دستیابی به شاخص­های رشد مطلوب در این گیاه سودمند است.


عنوان مقاله [English]

The Study of effects of spermine under salinity stress on morphophysiological ‎characteristics of Catharanthus roseus L.‎

نویسندگان [English]

  • Mitra Aelaei 1
  • Zahra Karami 2
  • Masood Arghavani 1
  • Fahimeh Salehi 3
1 Assistant Professor, Faculty of Agriculture, University of Zanjan, Zanjan, Iran,‎ ‎45371-38791‎
2 M.Sc. Student, Faculty of Agriculture, University of Zanjan, Zanjan, Iran,‎ ‎45371-38791‎
3 Ph.D. Candidate, Faculty of Agriculture, University of Zanjan, Zanjan, Iran,‎ 45371-38791‎
چکیده [English]

In order to study the effects of spermine in Catharanthus roseus L under salinity stress conditions, a factorial experiment was conducted in a completely randomized design pots containing 4:1 brown peat moss to perlite in greenhouse of Zanjan University. Treatments were four spermine at four levels (0, 5, 10 and 15 ppm) and salinity stresses at three levels (0, 60 and 120 mM) of sodium chloride. Traits which were studied were as fresh and dry weight, plant height, flower diameter, leaf number as morphological traits, relative water content, total chlorophyll, carotenoids as physiological traits, activity of antioxidant enzymes such as peroxidase and proline. Results showed that application of polyamine spermine improves growth and yield of Chatarantus roseus L in stress condition and on fresh and dry weight, plant height, flower number, relative water content, proline, chlorophyll content, carotenoid, peroxidase enzyme and leaf number at 1% and 5% probability levels. The highest proline content (0.37 µmol/g FW) was obtained in 10 ppm and the lowest (0.23 µmol/g FW) in control treatment. The highest peroxidase enzyme (0.099 units. g-1 FW.min-1) was obtained in 15 ppm spermine treatment which showed an increase in spermine compared to the control. Therefore, the use of spermine in this plant for achieving desirable growth indices is recommended and can be beneficial.

کلیدواژه‌ها [English]

  • Carotenoid
  • growth indices
  • Peroxidase
  • proline‎
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