بررسی ترکیب‌های شیمیایی اسانس جمعیت‌های طبیعی مورتلخ ‏‏(‏Salvia mirzayanii Rech. f. & Esfand.‎‏) در استان هرمزگان

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد، دانشکده کشاورزی و منابع طبیعی، دانشگاه هرمزگان، بندرعباس، ایران

2 استادیار، دانشکده کشاورزی و منابع طبیعی، دانشگاه هرمزگان، بندرعباس، ایران

3 دانشیار، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج، ایران

چکیده

مورتلخ (Salvia mirzayanii) یکی از گونه­های چندساله و معطر اندمیک و در حال انقراض از تیره نعناع (Lamiaceae) می­باشد که به‌صورت خودرو در جنوب ایران می­روید. در این پژوهش، سرشاخه­های گلدار گیاه مورتلخ از پنج رویشگاه در استان هرمزگان شامل دق­فینو، سرچاهان، بستک، سیرمند و تنگه­زاغ جمع­آوری گردید. نمونه­ها در سایه و دمای محیط، خشک شدند. استخراج اسانس به روش تقطیر با آب توسط دستگاه طرح کلونجر با سه تکرار انجام شد و پس از اندازه­گیری مقدار اسانس (درصد وزنی به وزنی)، نوع و مقدار ترکیبات شیمیایی موجود در اسانس جمعیت‌ها با استفاده از دستگاه­های گاز کروماتوگرافی گازی (GC) و گاز کروماتوگرافی متصل به طیف­سنج جرمی (GC/MS) تعیین گردید. نتایج نشان داد عملکرد اسانس جمعیت‌های دق­فینو، سرچاهان، بستک، سیرمند و تنگه­زاغ به ترتیب 3/1، 2/1، 1/1، 2/1 و 4/1 درصد (وزنی به وزنی) بودند. در مجموع تعداد 40 ترکیب در اسانس مورتلخ شناسایی گردید که از این تعداد، 28 ترکیب در تمام جمعیت‌ها مشترک بودند. بیشترین اجزای شیمیایی شناسایی شده در جمعیت‌های دق­فینو و بستک (36 ترکیب) و کمترین آنها در جمعیت سرچاهان (33 ترکیب) ملاحظه گردید. عمده­ترین ترکیبات تشکیل‌دهنده اسانس مورتلخ شامل دلتا- کادینن، لینالول، 1، 8- سینئول، آلفا- ترپینئول و گاما- کادینن در بین جمعیت‌های مورد بررسی بودند. وجود تفاوت بین جمعیت‌های مورتلخ نشان داد ضمن تأثیر وراثت گیاه، این گونه از پتاسیل سازگاری بالایی نسبت به شرایط اقلیمی مختلف برخوردار می­باشد.

کلیدواژه‌ها

عنوان مقاله [English]

Study on chemical compositions of essential oil of some Salvia mirzayanii Rech. f. & ‎Esfand. natural populations in Hormozgan province

نویسندگان [English]

  • Safiyeh Binava 1
  • Alireza Yavari 2
  • Majid Shokrpour 3
1 M.Sc. Student, College of Agriculture & Natural Resources, University of Hormozgan, Bandar Abbas, Iran
2 Assistant Professor, College of Agriculture & Natural Resources, University of Hormozgan, Bandar Abbas, Iran
3 Associate Professor, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
چکیده [English]

Salvia mirzayanii Rech. f. & Esfand. is an endemic medicinal plant which belonging to the Lamiaceae family where grows wild in Iran. In the present study, the aerial parts of this plant were collected at full flowering stage from four habitats in Hormozgan province including Dagh-e-Fino, Sarchahan, Bastak, Sirmand and Tang-e-Zagh. The plant materials were dried at shade and at room temperature. The essential oils were obtained by hydro-distillation with three replications, the yields were calculated based on dry weight and the oils were analyzed by GC and GC/MS. Results showed that the essential oil contents from Dagh-e-Fino, Sarchahan, Bastak, Sirmand and Tang-e-Zagh natural populations obtained 1.3, 1.2, 1.1, 1.2 and 1.4 (w/w %), respectively. A total of 40 compounds were identified in the essential oils of the aerial parts of S. mirzayanii, of which 28 compounds were common to all natural populations. The most identified chemical components were observed in Dagh-e-Fino and Bastak populations (36 compounds) and the lowest in Sarchahan population (33 compounds). Results of essential oil compound analysis revealed that δ-cadinene, linalool, 1,8-cineol, α-terpineol, γ-cadinene and α-terpinyl acetate were the major compounds among the essential oils of studied populations. Existence of differences among populations of S. mirzayanii showed that in addition to the effect of plant inheritance, it also has a high adaptation potential so that a wide range of climatic conditions.

کلیدواژه‌ها [English]

  • Chemical diversity
  • essential oil
  • natural habitat
  • δ-cadinene
  • Salvia mirzayanii‎

مراجع

  1. Adams, R.P. (2011). Identification of essential oils by ion trap mass spectroscopy. Academic Press.
  2. Afkar, S., Azadpour, M., Mahdavi, B. & Rashidipour, M. (2021). Evaluation of essential oil composition and antibacterial effect of Mentha longifolia ‎collected from different regions of Lorestan province. Iranian Journal of Horticultural Science, 51(4), 823-835. (In Farsi).
  3. Aprotosoaie, A.C., Hăncianu, M., Costache, I.I. & Miron, A. (2014). Linalool: a review on a key odorant molecule with valuable biological properties. Flavour and Fragrance Journal, 29(4), 193-219.
  4. Armana, M., Azizi, N. & Yousefzadi, M. (2012). Cytotoxicity, antimicrobial activity and composition of the essential oil from Salvia mirzayanii f. & Esfand from Iran. Journal of Biologically Active Products from Nature, 2(1), 54-58.
  5. Asadipour, A., Mehrabani, M., Moghaddasian, M., Ramazani, M., Amanzadeh, Y. & Saber-Amoli, S. (2004). Composition of the volatile oil of Salvia mirzayanii & Esphand from Iran. Journal of Essential Oil Bearing Plants, 7(2), 182-185.
  6. Asadollahi, M., Firuzi, O., Heidary, F., Jamebozorgi, M., Alizadeh, A. & Jassbi, R. (2018). Ethnopharmacological studies, chemical composition, antibacterial and cytotoxic activities of essential oils of eleven Salvia in Iran. Journal of Herbal Medicine and Toxicology, 18, 124-136.
  7. Bahadori, S., Sonboli, A. & Jamzad, Z. (2016). Anatomical and morphological characteristics of Salvia candidissima ssp. candidissima (Lamiaceae) as a new record from Iran. Iranian Journal of Botany, 22(2), 104-111.
  8. Bahadori, M.B., Salehi, P. & Sonboli, A. (2017). Comparative study of the essential oil composition of Salvia urmiensis and its enzyme inhibitory activities linked to diabetes mellitus and Alzheimer’s disease. International Journal of Food Properties, 20(12), 2974-2981.
  9. Binava, S., Yavari, A. & Shokrpour, M. (2020). A study on the quality and quantity of essential oil from different plant organs of Salvia mirzayaniiF. & Esfand. Iranian Journal of Medicinal and Aromatic Plants Research, 35(6), 914-924. (In Farsi).
  10. British Pharmacopoeia. (2007). Appendix XI. Vol. 2, London, HMSO, 137-138.
  11. Chowdhury, S. & Kumar, S. (2020). Alpha-terpinyl acetate: A natural monoterpenoid from Elettaria cardamomum as multi-target directed ligand in Alzheimer’s disease. Journal of Functional Foods, 68, 1380-1392.
  12. Davies, N.W. (1998). Gas chromatographic retention indices of monoterpenes and sesquiterpenes on methyl silicon and Carbowax 20M phases. Journal of Chromatography, 503, 1-24.
  13. Dudareva, N., Pichersky, E. & Gershenzon, J. (2004). Biochemistry of plant volatiles. Journal of Plant Physiology, 135, 1893-1902.
  14. Fattahi, B., Nazeri, V., Kalantari, S. & Bonfill, M. (2014). Identification of compounds in the essential oil and quantification of flavonoids and Rosmarinic acid in Salvia reuterana and Salvia palaestina Benth. Iranian Journal of Medicinal and Aromatic Plants Research, 30(3), 463-475. (In Farsi).
  15. Ghasemi, E., Sharafzadeh, Sh., Amiri, B., Alizadeh, A. & Bazrafshan, F. (2020). Variation in essential oil constituents and antimicrobial activity of the flowering aerial parts of Salvia mirzayanii & Esfand. ecotypes as a folkloric herbal remedy in southwestern Iran. Journal of Essential Oil Bearing Plants, 23(1), 51-64.
  16. Govindarajan, M., Rajeswary, M. & Benelli, G. (2016). δ-Cadinene, calarene and δ-4-carene from Kadsura heteroclita essential oil as novel larvicides against malaria, dengue and filariasis mosquitoes. Combinatorial Chemistry & High Throughput Screening, 19(7), 565-571.
  17. Gupta, R.C., Doss, R.B., Srivastava, A., Lall, R. & Sinha, A. (2019). Nutraceuticals for control of ticks, fleas, and other ectoparasites. Nutraceuticals in Veterinary Medicine, 43, 625-633.
  18. Heydari, Z., Yavari, A., Jafari, L. & Mumivand, H. (2020). Study on the chemical diversity of essential oil from different plant parts of Salvia sharifii f. & Esfand. Iranian Journal of Medicinal and Aromatic Plants Research, 36(4), 627-641. (In Farsi).
  19. Hui, L.M., Zhao, G.D. & Zhao, J.J. (2015). δ-Cadinene inhibits the growth of ovarian cancer cells via caspase-dependent apoptosis and cell cycle arrest. International Journal of Clinical and Experimental Pathology, 8(6), 6046-6056.
  20. Jamzad, Z. (2012). Flora of Iran: Lamiaceae. Research Institute of Forests and Rangelands of Iran. 1074 p. (In Farsi).
  21. Javidnia, K., Miri, R., Kamalinejad, M. & Nasiri, A. (2002). Composition of the essential oil of Salvia mirzayanii f. & Esfand from Iran. Flavour and Fragrance Journal, 17, 465-467.
  22. Llusia, J., Penuelas, J., Alessio, G.A. & Estiarte, M. (2006). Seasonal contrasting changes of foliar concentrations of terpenes and other volatile organic compound in four dominant species of a Mediterranean shrubland submitted to a field experimental drought and warming. Physiologia Plantarum, 127, 632-649.
  23. Mahdieh, M., Talebi, S.M. & Akhani, M. (2018). Infraspecific essential oil and anatomical variations of Salvia nemorosa (Labiatae) populations in Iran. Industrial Crops and Products, 123, 35-45.
  24. Meamari, S., Yavari, A. & Bikdeloo, M. (2020). Investigation of chemical diversity of essential oil of natural populations of Zataria multiflora in Hormozgan province. Iranian Journal of Horticultural Science, 51(3), 669-677. (In Farsi).
  25. Medjahed, F., Merouane, A., Saadi, A., Bader, A., Cioni, P.L. & Flamini, G. (2016). Chemical profile and antifungal potential of essential oils from leaves and flowers of Salvia algeriensis (Desf.): A comparative study. Chilean Journal of Agricultural Research, 76(2), 195-200.
  26. Mirza, M., Baher Nik, Z. & Jamzad, Z. (2003). The Extraction and identification of the essential oil constituents of Salvia mirzayanii f. & Esfand. Iranian Journal of Medicinal and Aromatic Plants Research, 19(2), 117-124. (In Farsi).
  27. Mozaffarian, V. (2007). A dictionary of Iranian plants names. Farhang Moaser. (In Farsi).
  28. Nakamura, A., Fujiwara, S., Matsumoto, I. & Abe, K. (2009). Stress repression in restrained rats by (R)-(-)-linalool inhalation and gene expression profiling of their whole blood cells. Journal of Agricultural and Food Chemistry, 57, 5480-5485.
  29. Nematollahi, A., Mirjalili, M.H., Hadian, J. & Yousefzadi, M. (2017). Chemical diversity among the essential oils of natural Salvia mirzayanii (Lamiaceae) populations from Iran. Plant Production Technology, 9(1), 1-16. (In Farsi).
  30. Omidbaigi, R. (2009). Production and processing of medicinal plants. Astane ghodse Razavi. (In Farsi).
  31. Rajabi, Z., Ebrahimi, M., Farajpour, M., Mirza, M. & Ramshini, H. (2014). Compositions and yield variation of essential oils among and within nine Salvia species from various areas of Iran. Industrial Crops and Products, 61, 233-239.
  32. Raeisi Monfared, A., Yavari, A. & Moradi, N. (2018). Study on chemical compositions of essential oil of some Salvia santolinifolia ecotypes. Iranian Journal of Horticultural Science, 50(3), 745-754. (In Farsi).
  33. Raguso, R.A. (2016). More lessons from linalool: insights gained from a ubiquitous floral volatile. Current Opinion in Plant Biology, 32, 31-36.
  34. Resetnik, I., Baricevic, D., Batıˆr Rusu, D., Carovic´-Stanko, K., Chatzopoulou, P., Dajic´-Stevanovic´, Z., Gonceariuc, M., Grdisˇa, M., Gregurasˇ, D, Ibraliu, A., Jug-Dujakovic´, M., Krasniqi, E., Liber, Z., Murtic´, S., Pec´anac, D., Radosavljevic´, I., Stefkov, G., Stesˇevic´, D., Sosˇtaric, I., Varbanova, K. & Satovic, Z. (2016). Genetic diversity and demographic history of wild and cultivated/naturalised plant populations: evidence from Dalmatian sage (Salvia officinalis, Lamiaceae). PLoS One, 11, 1-23.
  35. Rechinger, K.H. (1982). Flora Iranica. Graz: Akademische Druck- und Verlagsanstalt.
  36. Sangwan, N.S., Farooqi, A.H.A., Shabih, F. & Sangwan, R.S. (2001). Regulation of essential oil production in plants. Plant Growth Regulation, 34, 3-21.
  37. Sefidkon, F. & Khajavi, M.S. (1999). Chemical composition of the essential oils of two Salvia species from Iran, Salvia verticillata and Salvia santolinifolia Boiss. Flavour and Fragrance Journal, 14, 77-78.
  38. Shibamoto, T. (1987). Retention indices in essential oil analysis in capillary gas chromatography in essential oil analysis. Alfred Heuthig.
  39. Soltanipoor, M.A. (2004). Phenolological study of Salvia mirzayaniif. & Esfand in different height regions of Hormozgan province. Pajoush and Sazandegi, 17(4), 34-38. (In Farsi).
  40. Soltanipoor, M.A. (2007). Investigation on relationship between ecological factors and natural distribution and density of Salvia mirzayanii & Esfand. medicinal species in Hormozgan province. Iranian Journal of Medicinal and Aromatic Plants, 23(2), 218-225. (In Farsi).
  41. Sonboli, A., Kanani, M.R. & Mojarad Ashna Abad, M. (2006). Comparison of the essential oil composition of Salvia santolinifolia in three localities from Iran. Iranian Journal of Medicinal and Aromatic Plants, 22(2), 128-134. (In Farsi).
  42. Taheri Boukani, K. & Najafzadeh, R. (2020). Evaluation of diversity of yarrow (Achillea biebersteinii and Achillea ‎wilhelmsii C. Koch) populations in West-Azerbaijan province based on ‎morphological traits, essential oil percentage and composition. Iranian Journal of Horticultural Science, 51(1), 215-228. (In Farsi).
  43. Vaičiulytė, V., Ložienė, K., Švedienė, J., Raudonienė, V. & Paškevičius, A. (2021). α-Terpinyl Acetate: Occurrence in essential oils bearing Thymus pulegioides, phytotoxicity, and antimicrobial effects. Molecules, 26(4), 106-122.
  44. Yavari, A., Nazeri, V., Sefidkon, F. & Hassani, M.E. (2010). Influence of some environmental factors on the essential oil variability of Thymus migricus. Natural Product Communications, 5(6), 943-948.
  45. Yamini, Y., Khajeh, M., Ghasemi, E., Mirza, M. & Javidnia, K. (2008). Comparison of essential oil compositions of Salvia mirzayanii obtained by supercritical carbon dioxide extraction and hydrodistillation methods. Food Chemistry, 108(1), 341-346.
  46. Zarshenas, M.M. & Krenn, L. (2014). Phytochemical and pharmacological aspects of Salvia mirzayanii f. & Esfand. Journal of Evidence-Based Integrative Medicine, 20(1), 65-72.

 

علوم باغبانی ایران
دوره 52، شماره 2
شهریور 1400
صفحه 391-403

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سابقه مقاله

  • تاریخ دریافت: 22 فروردین 1400
  • تاریخ بازنگری: 18 اردیبهشت 1400
  • تاریخ پذیرش: 22 اردیبهشت 1400

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