بررسی روند برخی تغییرات بیوشیمیایی و فیزیولوژیکی در برگ رقم‌های زیتون (‏Olea europaea ‎L.‎‏) طی دوره سازگاری و عدم سازگاری به سرما

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی سابق دکتری، دانشکده‎ ‎کشاورزی،‎ ‎دانشگاه‎ ‎صنعتی‎ ‎اصفهان،‎ ‎اصفهان

2 دانشیار، دانشکده‎ ‎کشاورزی،‎ ‎دانشگاه‎ ‎صنعتی‎ ‎اصفهان،‎ ‎اصفهان

3 استاد، دانشکده‎ ‎کشاورزی،‎ ‎دانشگاه‎ ‎صنعتی‎ ‎اصفهان،‎ ‎اصفهان

10.22059/ijhs.2020.297583.1768

چکیده

این پژوهش به منظور بررسی تغییرات در سطح کربوهیدرات­های محلول برگ، ظرفیت آنتی اکسیدانی، فنول کل، چگالی برگ، محتوای نسبی آب برگ، ماده خشک برگ و اسیدهای چرب در طی دوره سازگاری و عدم سازگاری به سرما و ارتباط آن با تحمل به یخ‌زدگی زیتون انجام شد. سه رقم زیتون آمفی­سیس، گرگان و مانزانیلا انتخاب و نمونه­های برگی از باغ زیتون در طی پاییز تا تابستان در شش زمان (آبان، آذر، دی، بهمن، فروردین و تیر ماه) تهیه شدند. به منظور تعیین تحمل به یخ‌زدگی نمونه­های برگی به مدت 12 ساعت در معرض انجماد مصنوعی (صفر تا 25- درجه سلسیوس) قرار گرفتند. نتایج نشان داد بین رقم­های زیتون از نظر تحمل به یخ‌زدگی در هر مرحله از نمونه­برداری تفاوت معنی‌داری مشاهده شد. تحمل به یخ‌زدگی رقم­ها طی مرحله سازگاری به سرما افزایش و در مرحله خروج از سازگاری کاهش یافت. کربوهیدرات­های محلول، ظرفیت آنتی‌اکسیدانی، محتوای فنول کل و درصد ماده خشک برگ، به ویژه از مرحله شروع سازگاری به سرما تا سازگاری کامل به سرما در دی ماه در رقم متحمل به یخ‌زدگی -آمفی­سیس- بیشتر از رقم­های گرگان و مانزانیلا بود. بیشترین نسبت اسید­های چرب غیر اشباع به اشباع در هر دو ماه دی و تیر در رقم آمفی­سیس مشاهده شد. بنابراین از ترکیباتی مانند کربوهیدرات­های محلول، محتوای فنول کل و اسید­های چرب، می‌توان به عنوان شاخص­های بیوشیمیایی تعیین کننده تحمل به یخ‌زدگی در غربالگری رقم­های زیتون استفاده کرد.

کلیدواژه‌ها


عنوان مقاله [English]

Investigation of some biochemical and physiological changes in leaves of olive (Olea ‎europaea L.) cultivars during cold acclimation and de-acclimation stage ‎

نویسندگان [English]

  • Safoora Saadati 1
  • Bahram Bani Nasab 2
  • Mostafa Mobli 3
  • Mahdiyeh Gholami 2
1 Former Ph.D. Student,, College of Agriculture, Isfahan University of Technology, Isfahan, Iran‎
2 Associate Professor, College of Agriculture, Isfahan University of Technology, Isfahan, Iran‎
3 Professor, College of Agriculture, Isfahan University of Technology, Isfahan, Iran‎
چکیده [English]

This study was conducted to investigate the changes in the levels of soluble carbohydrate, antioxidant capacity, total phenolic acid and fatty acid composition of olive during cold acclimation and deacclimation related with freezing tolerance (FT). Three olive cultivars, Amphisis, Gorgan and Manzanilla were selected and leaf samples were prepared from field-grown trees from autumn to summer at six times (November, December, December, February, April and July). The FT was measured by exposure to artificial freezing (0ºC to –25ºC). Result showed that there was a significant difference between the olive cultivars in terms of FT at each stage of sampling. The FT of cultivars increased during the cold acclimation stage and declined in deacclimation stage. Soluble carbohydrates, antioxidant capacity, total phenolic content, leaf dry matter, especially from the beginning of cold acclimation until full acclimation, in cold-tolerant –Amphisis– cultivar was more than the Gorgan and Manzanilla cultivars. The highest ratio of unsaturated to saturated fatty acids in both January and July was observed in Amphisis cultivar. Therefore, compounds such as soluble carbohydrates, total phenolic content, and fatty acids can be used as biochemical indicator of FT in screening of olive cultivars.

کلیدواژه‌ها [English]

  • Fatty acids
  • Freezing tolerance
  • soluble carbohydrates
  • total phenolic content
  1. Arias, N. S., Bucci, S. J., Scholz F. G. & Goldstein, G. (2015). Freezing avoidance by supercooling in Olea europaea cultivars: the role of apoplastic water, solute content and cell wall rigidity. Plant, Cell and Environment, 38, 2061–2070.
  2. Ashworth, E. N. & Abeles, F. B. (1984). Freezing behavior of water in small pores and the possible role in the freezing of plant tissues. Plant Physiology, 76, 201–204.
  3. Balasundram, N., Sundram, K. & Samman, S. (2006). Phenolic compounds in plants and agri–industrial by–products: Antioxidant activity, occurrence, and potential uses. Food Chemistry, 99, 191–203.
  4. Bartolozzi, F., Mencuccini, M. & Fontanazza, G. (2001). Enhancement of frost tolerance in olive shoots in vitro by cold acclimation and sucrose increase in the culture medium. Plant Cell, Tissue and Organ Culture, 67, 299–302.
  5. Bligh, E.G. & Dyer, W. J. (1959). A rapid method of total lipid extraction and purification. Canadian Journal of Biochemistry and Physiology, 37, 911–917.
  6. Cansev, A., Gulen, H. & Eris, A. (2009). Cold–hardiness of olive (Olea europaea) cultivars in cold–acclimated and non–acclimated stages: seasonal alteration of anti–oxidative enzymes and dehydrin–like proteins. The Journal of Agricultural Science, 147, 51–61.
  7. Ebrahimzadeh, M. A., Hosseinimehr, S. J. & Hamidinia, A. (2008). Antioxidant and free radical scavenging activity of Feijoa sallowiana fruits peel and leaves. Pharmacologyonline, 1, 7–14.
  8. Eris, A., Gulen, H., Barut, E. & Cansev, A. (2007). Annual patterns of total soluble sugars and proteins related to cold hardiness in olive (Olea europaea Gemlik). The Journal of Horticultural Science and Biotechnology, 82, 597–604.
  9. Ershadi, A., Karimi, R. & Mahdei, K. N. (2016). Freezing tolerance and its relationship with soluble carbohydrates, proline and water content in 12 grapevine cultivars. Acta Physiologiae Plantarum, 38, 2.
  10. Gao, X., Ohlander, M., Jepsson, N., Bjork, L. & Trajkovski, V. (2000). Changes in antioxidamt effects and their relationship to phytonutrients in fruits of sea buckthorn (Hippophae rhamnoides) during maturation. Journal of Agricultural and Food Chemistry, 48, 1485–1490.
  11. Ghasemi Soloklui, A. A., Ershadi, A. & Fallahi, E. (2012). Evaluation of cold hardiness in seven Iranian commercial pomegranate (Punica granatum) cultivars. HortScience, 47, 1821–1825.
  12. Goli, S. A. H., Sahri, M. M. & Kadivar, M. (2008). Enzymatic interesterification of structured lipids containing conjugated linoleic acid with palm stearin for possible margarine production. European Journal of Lipid Science and Technology, 110, 1102–1108.
  13. Gulen, H., Cansev, A. & Eris, A. (2009). Cold hardiness of olive (Olea europaea) cultivars in cold–acclimated and non–acclimated stages: seasonal alteration of soluble sugars and phospholipids. The Journal of Agricultural Science, 147, 459–467.
  14. Gusta, L. V., Wisniewski, M., Nesbitt, N. T. & Gusta, M. L. (2004). The effect of water, sugars, and proteins on the pattern of ice nucleation and propagation in acclimated and non–acclimated canola leaves. Plant Physiology, 135, 1642–1653.
  15. Hashempour, A., Ghasemnezhad, M., Fotouhi Ghazvini, R. & Sohani, M. M. (2014). Olive (Olea europaea ) freezing tolerance related to antioxidant enzymes activity during cold acclimation and non acclimation. Acta Physiologiae Plantarum, 36, 3231–3241.
  16. Huang, Y. & Wang, Z. (1982). Cytological determination of cold resistance in fruit trees (Malus). Acta Horticulturae Sinica, 9, 23–30.
  17. Hubackova, M. (1982). Effect of the lignification of grapevine shoots on the resistance of buds in winter. Vitis, 9, 271–274.
  18. Irigoyen, J. J., Emerich, D. W. & Sanchez–Diaz. M. (1992). Water stress induced changes in concentration of proline and total soluble sugars in nodulated alfalfa (Medicago sativa) plants. Physiologia Plantarum, 84, 55–60.
  19. Jiang, H. & Howell, G. (2002). Correlation and regression analyses of cold hardiness, air temperatures, and water content of Concord grapevines. American Journal of Enology and Viticulture, 53, 227–230.
  20. Johnson–Flanagan, A. M. & Owens, J. N. (1985). Peroxidase activity in relation to suberization and respiration in white spruce (Picea glauca [Moench] Voss) seedling roots. Plant Physiology, 79, 103–107.
  21. Joyce, C., Sam, C. & Cecil, S. (2005). Relationship of cold acclimation, total phenolic content and antioxidant capacity with chilling tolerance in petunia (Petunia × hybrid). Environmental and Experimental Botany, 53, 225–232.
  22. Mancuso, S. (2000). Electrical resistance changes during exposure to low temperature measure chilling and freezing tolerance in olive tree (Olea europaea) plants. Plant, Cell and Environment, 23, 291-299.
  23. Moradi Heidarabad, S. & Ershadi, A. (2021). Evaluation of some physiological and biochemical responses of seven commercial grape cultivars to cold stress during the growing season. Iranian Journal of Horticultural Science, 52, 213–224. (in Farsi).
  24. Palta, J. P., Whitaker, B. D. & Weiss, L. S. (1993). Plasma membrane lipids associated with genetic variability in freezing tolerance and cold acclimation of Solanum Plant Physiology, 103, 793–803.
  25. Pennycooke, J. C., Cox, S. & Stushnoff, C. (2005). Relationship of cold acclimation, total phenolic content and antioxidant capacity with chilling tolerance in petunia (Petunia× hybrida). Environmental and Experimental Botany, 53, 225–232.
  26. Proietti, P. & Famiani, F. (2002). Diurnal and seasonal changes in photosynthetic characteristics in different olive (Olea europaea) cultivars. Photosynthetica, 40, 171–176.
  27. Rajashekar, C. B. & Burke, M. J. (1996). Freezing characteristics of rigid plant tissues. Development of cell tension during extracellular freezing. Plant Physiology, 111, 597–603.
  28. Rice–Evans, C.A., Miller, N.J. & Paganga, G. (1997). Antioxidant properties of phenolic compounds. Trends in Plant Science, 2, 152–159.
  29. Rosa, R. D., Rallo, L. & Rapoport, H.F. (2000). Olive floral bud growth and starch content during winter rest and spring budbreak. HortScience, 35, 1223–1227.
  30. Scholz, F. G., Bucci, S. J., Goldstein, G., Meinzer, F., Franco, A. C. & Miralles–Wilhelm, F. (2007). Biophysical properties and functional significance of stem water storage tissues in Neotropical savanna Plant, Cell and Environment, 30, 236–248.
  31. Shojaee, K., Davarynejad, G. & Nezami, A. (2012). An investigation on freezing tolerance of peach and nectarine reproductive buds in controlled conditions. Iranian Journal of Horticultural Science, 43, 13– (in Farsi).
  32. Stefanowska, M., Kuraś, M., Kubacka–Zebalska, M. & Kacperska, A. (1999). Low temperature affects pattern of leaf growth and structure of cell walls in winter oilseed rape (Brassica napus, var. oleifera L.). Annals of Botany, 84, 313–319.
  33. Sugiyama, S. & Shimazaki, T. (2007). Increased cell–wall mass and resistance to freezing and snow mold during cold acclimation of winter wheat under field conditions. Plant Production Science, 10, 383–390.
  34. Vyse, K., Pagter, M., Zuther, E. & Hincha, D. K. (2019). Deacclimation after cold acclimation-a crucial, but widely neglected part of plant winter survival. Journal of Experimental Botany, 70, 4595–4604.
  35. Weatherley, P. (1950). Studies in the water relations of the cotton plant. The field measurement of water deficit in leaves. New Phytologist, 49, 81–97.
  36. Wilson, J. M. (1972). The mechanism of chill–and drought–hardening of Phaseolus vulgaris New Phytologist, 76, 257–269.