بررسی تأثیر تنش مولیبدن بر عملکرد سیستم آنتی‌اکسیدان گیاهچه‌های جعفری ‏‏(‏Petroselinum sativum L.‎‏) در شرایط آزمایشگاهی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانش‌آموخته کارشناسی ارشد، دانشکده علوم، دانشگاه فردوسی مشهد، مشهد، ایران

2 استاد، دانشکده علوم، دانشگاه فردوسی مشهد، مشهد، ایران

3 استادیار، دانشکده علوم، دانشگاه فردوسی مشهد، مشهد، ایران

چکیده

ازآنجایی‌که خاک­ های کشاورزی در اکثر نقاط دنیا به مقادیر متفاوتی از فلزات سنگین آلوده هستند، تنش فلزات سنگین یکی از تنش‌های اصلی در این مناطق محسوب می­ گردد که می ­تواند تأثیر منفی بر میزان تولید محصولات گیاهی داشته باشد. در این پژوهش تأثیر تنش مولیبدن [سطح شاهد (C: 9/1میکرومولار) و سطوح تیماری 5/0+C، 5/2+C، 5+C، 10+C میکرومولارمولیبدات سدیم اضافه شده به محلول غذایی هوگلند] بر رشد و عملکرد سیستم آنتی‌اکسیدان آنزیمی و غیرآنزیمی گیاه جعفری درمرحله گیاهچه ­ای (پنج­ هفته­ ای) مورد ارزیابی قرار گرفت. نتایج نشان داد هرچند سطوح مختلف تنش مولیبدن موجب کاهش وزن خشک و طول بخش هوایی و ریشه گیاهچه‌ها و همچنین کاهش شاخص ­پایداری ­غشا و محتوای­ نسبی آب برگ آن­ها شد، ولیکن، اثر افزایش­دهنده بر محتوای پرولین (افزایش 150 درصدی محتوای پرولین بخش­هوایی نسبت به شاهد) و ترکیبات­ فنلی (افزایش 80 و 85 درصدی محتوای فنل­کل بخش­ هوایی و ریشه نسبت به شاهد) داشت. فعالیت آنزیم­های آنتی‌اکسیدان کاتالاز، بنزیدین­پراکسیداز، آسکوربات­ پراکسیداز و پلی­ فنل­ اکسیداز هم بخش­هوایی و هم ریشه، در شرایط مواجه با تنش مولیبدن افزایش یافتند. بنابراین این‌طور می‌توان نتیجه ­گیری کرد که گیاهچه ­های جعفری سعی می­ کنند از مسیر کاهش نسبی فرایند رشد و به عوض آن، افزایش سیستم­ دفاع آنتی‌اکسیدان به "تحمل" شرایط تنش فلزسنگین مولیبدن در این مرحله نموی بپردازد.

کلیدواژه‌ها

عنوان مقاله [English]

Effects of molybdenum stress on antioxidant system performance of parsley ‎seedlings (Petroselinum sativum L.) under laboratory condition

نویسندگان [English]

  • Sedigheh Barati 1
  • Mehrdad Lahouti 2
  • Monireh Cheniany 3
1 Former M. Sc. Student,, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran
2 Professor, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran
3 Assistant Professor, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran
چکیده [English]

Since agricultural soils in many parts of the world are contaminated with varying amounts of heavy metals, heavy metal stress is one of the major issues in these areas that can have a negative effects on crop production. In this study, the effect of different levels of molybdenum [Control (C: 1.9 µM), C+0.5, C+2.5, C+5, C+10 µM sodium molybdate] was evaluated on the growth and function of enzymatic and non-enzymatic antioxidant system of parsley at seedling stage. The results showed that although the molybdenum stress decreased dry weight and length of both plant parts (shoot and root) as well as membrane stability index and relative water content, it had an increasing effect on proline (150% increase in proline content of shoot compared to control) and phenolic compounds (80% and 85% increase in phenolic content of shoot and root, compared to control). The activity of antioxidant enzymes catalase, benzidine peroxidase, ascorbate peroxidase and polyphenoloxidase was also increased under molybdenum stress. Therefore, it may be concluded that parsley seedlings try to "tolerate" the stress condition of molybdenum heavy metal at this physiological-growth stage by partially reducing the growth process and, in turn, enhancing its antioxidant defense systems.

کلیدواژه‌ها [English]

  • Enzymatic antioxidant system
  • heavy metal
  • molybdenum stress
  • parsley
  • proline

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علوم باغبانی ایران
دوره 52، شماره 2
شهریور 1400
صفحه 281-291

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سابقه مقاله

  • تاریخ دریافت: 28 شهریور 1398
  • تاریخ بازنگری: 24 آبان 1398
  • تاریخ پذیرش: 08 آذر 1398

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