بررسی تأثیر تنش مولیبدن بر عملکرد سیستم آنتی‌اکسیدان گیاهچه‌های جعفری ‏‏(‏Petroselinum sativum L.‎‏) در شرایط آزمایشگاهی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانش‌آموخته کارشناسی ارشد، دانشکده علوم، دانشگاه فردوسی مشهد، مشهد، ایران

2 استاد، دانشکده علوم، دانشگاه فردوسی مشهد، مشهد، ایران

3 استادیار، دانشکده علوم، دانشگاه فردوسی مشهد، مشهد، ایران

چکیده

ازآنجایی‌که خاک­ های کشاورزی در اکثر نقاط دنیا به مقادیر متفاوتی از فلزات سنگین آلوده هستند، تنش فلزات سنگین یکی از تنش‌های اصلی در این مناطق محسوب می­ گردد که می ­تواند تأثیر منفی بر میزان تولید محصولات گیاهی داشته باشد. در این پژوهش تأثیر تنش مولیبدن [سطح شاهد (C: 9/1میکرومولار) و سطوح تیماری 5/0+C، 5/2+C، 5+C، 10+C میکرومولارمولیبدات سدیم اضافه شده به محلول غذایی هوگلند] بر رشد و عملکرد سیستم آنتی‌اکسیدان آنزیمی و غیرآنزیمی گیاه جعفری درمرحله گیاهچه ­ای (پنج­ هفته­ ای) مورد ارزیابی قرار گرفت. نتایج نشان داد هرچند سطوح مختلف تنش مولیبدن موجب کاهش وزن خشک و طول بخش هوایی و ریشه گیاهچه‌ها و همچنین کاهش شاخص ­پایداری ­غشا و محتوای­ نسبی آب برگ آن­ها شد، ولیکن، اثر افزایش­دهنده بر محتوای پرولین (افزایش 150 درصدی محتوای پرولین بخش­هوایی نسبت به شاهد) و ترکیبات­ فنلی (افزایش 80 و 85 درصدی محتوای فنل­کل بخش­ هوایی و ریشه نسبت به شاهد) داشت. فعالیت آنزیم­های آنتی‌اکسیدان کاتالاز، بنزیدین­پراکسیداز، آسکوربات­ پراکسیداز و پلی­ فنل­ اکسیداز هم بخش­هوایی و هم ریشه، در شرایط مواجه با تنش مولیبدن افزایش یافتند. بنابراین این‌طور می‌توان نتیجه ­گیری کرد که گیاهچه ­های جعفری سعی می­ کنند از مسیر کاهش نسبی فرایند رشد و به عوض آن، افزایش سیستم­ دفاع آنتی‌اکسیدان به "تحمل" شرایط تنش فلزسنگین مولیبدن در این مرحله نموی بپردازد.

کلیدواژه‌ها


عنوان مقاله [English]

Effects of molybdenum stress on antioxidant system performance of parsley ‎seedlings (Petroselinum sativum L.) under laboratory condition

نویسندگان [English]

  • Sedigheh Barati 1
  • Mehrdad Lahouti 2
  • Monireh Cheniany 3
1 Former M. Sc. Student,, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran
2 Professor, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran
3 Assistant Professor, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran
چکیده [English]

Since agricultural soils in many parts of the world are contaminated with varying amounts of heavy metals, heavy metal stress is one of the major issues in these areas that can have a negative effects on crop production. In this study, the effect of different levels of molybdenum [Control (C: 1.9 µM), C+0.5, C+2.5, C+5, C+10 µM sodium molybdate] was evaluated on the growth and function of enzymatic and non-enzymatic antioxidant system of parsley at seedling stage. The results showed that although the molybdenum stress decreased dry weight and length of both plant parts (shoot and root) as well as membrane stability index and relative water content, it had an increasing effect on proline (150% increase in proline content of shoot compared to control) and phenolic compounds (80% and 85% increase in phenolic content of shoot and root, compared to control). The activity of antioxidant enzymes catalase, benzidine peroxidase, ascorbate peroxidase and polyphenoloxidase was also increased under molybdenum stress. Therefore, it may be concluded that parsley seedlings try to "tolerate" the stress condition of molybdenum heavy metal at this physiological-growth stage by partially reducing the growth process and, in turn, enhancing its antioxidant defense systems.

کلیدواژه‌ها [English]

  • Enzymatic antioxidant system
  • Heavy metal
  • molybdenum stress
  • Parsley
  • proline
  1. Aksoy, M. & Dinler, B. S. (2012). Changes in physiological parameters and some antioxidant enzymes activities of soybean (Glycine max) leaves under cadmium and salt stress. Journal of Stress Physiology & Biochemistry, 8(4), 179 – 190.
  2. Andrson, S. (2003). Basic information about molybdenum as plant nutrient. Plant Cell and Environment, 24, 1271-1278.
  3. Arnon, D. J. (1956). Chlorophyll absorption spectrum and quantitative determination. Biochemical and Biophysical Acta, 20, 449-461.
  4. Arrigoni, O. L., Gara, F., Tommasi, R. & Lis, O. (1992). Change in ascorbate system during seed development of Vicia faba. Plant Physiology, 99, 235-238.
  5. Assche, F. & Cliysters, H. (1990). Effect of metals on enzyme activity in plants. Plant, Cell and Environment, 13(3), 195-206.
  6. Azizpour, K., Shakiba, M. R., Khosh Kholgh Sima, N., Alyari, H., Moghaddam, M., Esfandiari, E. & Pessarakli, M. (2010). Physiological response of spring durum wheat genotypes to salinity. Journal of Plant Nutrition, 33, 859-873.
  7. Bates, L. S., Waldren, R. P. & Tear, I. D. (1973). Rapid determination of free proline for water stress studies. Plant and Soil, 39, 205-207.
  8. Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of micro gram quantities of protein utilizing the principle of protein dye binding. Analytical Biochemistry, 72, 248-254.
  9. Cakmak, I. & Horst, W. J. (1991). Effect of aluminum on lipid peroxidation, superoxide dismutase, catalase and peroxidase activities in root tips of soybean (Glycine max). Physiologia Plantarum, 83, 463-468.
  10. Cenkci, S., Cioerci, I. H., Yildiz, M., Oezay, C., Bozdao, A. & Terzi, H. (2010). Lead contamination reduces chlorophyll biosynthesis and genomic template stability in (Brassica rapa). Environmental and Experimental Botany, 67, 467-473.
  11. Fariduddin, Q., Hayat, S. & Ahmad, A. (2003). Salicylic acid influences net photosynthetic rate, carboxylation efficiency, nitrate reductase activity and seed yield in Brassica juncea. Photosynthetica, 41, 281-284.
  12. Gardea-Torresdey, J., Peralta-Videa, J., Montes, M., Role, G. & Corral-Diaz, B. (2004). Bioaccumulation of cadmium, chromium and copper by (Convolvulus arvensis) Impact on plant growth and uptake of nutritional elements. Bio resource Technology, 92, 229-235.
  13. Heath, R. L. & Packer, L. (1969). Photo peroxidation in isolated chloroplast, 1. Kinetics and stoichiometry of fatty acid peroxidation. Archives of Biochemistry and Biophysics,125, 189-198.
  14. Hogland, D. R. & Arnon, D. I. (1950). The water culture method for growing plants without soil. California Agricultural Experiment Station, Circular, 347.
  15. Irani, M., Sarmadi, M., Bernard, F. & Shakerbazarno, H. (2008). Effect of molybdenum stress on anthocyanin, protein, malondialdehyde content and peroxidase activity in two varieties of licorice (Glycyrrhiza glabra) under in vitro culture. Special Issue on Pajouhesh & Sazandegi, (8), 20-13 (In Farsi).
  16. Jung, CH., Maeder, V., Funk, F. & Frey, B. (2003). Release of phenols from Lupinus albus roots exposed to Cu and their possible role in Cu detoxification. Plant Soil, 252 (2), 301-312.
  17. Karamian, R., Asadbeigy, M. & Yari, S. (2017). Antioxidant activity of Glycyrrhiza glabra extract and protective effect of its leaf extract on ethanol-induced nephrotoxicity in male rats. Scientific Journal of Ilam University of Medical Sciences, 26(4), 1-12.
  18. Khavari-Nejad, R. A., Goshehgir, Z. & Sa'adatman, S. (2010). The effects of selenium molybdenum interaction on contents of photosyntetic pigments in tomato (Lycopersicum esculentum). Journal of Plant Science Researches, 30, 711-719.
  19. Kuznetsov, V. & Shevyakova, N. I. (1999). Proline under stress: Biological role, metabolism and regulation. Journal of Plant Physiology, 46 (2), 247-287.
  20. Li, J. & Li, X. D. (2002). Effect of copper and molybdenum on quality in pakchoi. Fujian Agricultural Science and Technology, 3, 13-14.
  21. Lichtenthaler, H. K. (1987). Chlorophyll fluorescence signature of leaves during the autumnal chlorophyll breakdown. Journal of Plant Physiology, 131, 101-110.
  22. Liu, P., Yang, Y. S., Xu, G. D., Fang, Y. H. & Yang, Y. A. (2005). The response of antioxidant enzymes of three soybean varieties to molybdenum and boron in soil with a connection to plant quality. Plant, Soil and Environment, 51(8), 351-359.
  23. Martins, L. L. & Mourato, M. P. (2006). Effect of excess copper on tomato plants growth parameters, enzyme activities, chlorophyll, and mineral content. Journal of Plant Nutrition, (12), 2179-2198.
  24. McKersie, B. D. & Leshem, Y. (1994). Stress and stress coping in cultivated plants. (pp. 1-180.) Kluwer Academic Publish.
  25. Michalak, A. (2006). Phenolic compounds and their antioxidant activity in plants growing under heavy metal stress. Polish Journal of Environmental Studies, 15(4), 523-530.
  26. Mittler, R., Vanderauwera, S., Gollery, M. & Van Breusegem, F. (2004). Reactive oxygen gene network of plants. Trends in Plant Science, 9 (10), 490-498.
  27. Ovecka, M. & Takac, T. (2014). Managing heavy metal toxicity stress in plant: biological and biotechnological tools, Biotechnoligy Advances, 32(1), 73-86.
  28. Pourakbar, L., Khayami, M., Khara, J. & Farbodina, T. (2007). Copper-Induse change in antioxidative system in maize (Zea mays). Pakistan Journal of Biological Sciences, 10, 3662-3667.
  29. Putter, J. (1974). In: methods of enzymatic analysis (2nd ed.). Weinhan.
  30. Rajeev, G., Yogesh, K., Sharma, K. & Arvind, K. (2015). Effect of molybdenum stress on growth, yield and seed quality in black gram. Journal of Plant Nutriation, 48, 879-886.
  31. Raymond, M., Poulin, E., Boirox, V., Dupont, E & Pasteur N. (1993). Stability of insecticide resistance due to amplification of esterase genes in Cluex pipiens. Heredity, 70, 301-307.
  32. Rout, G.R. & Das, P. (2002). Rapid hydroponic screening for molybdenum tolerance in rice through morphological and biochemical analysis. Rostlinna Vyroba, 48(11), 505-512.
  33. Sairam, R. K. & Saxena, D.C. (2000). Oxidative stress and antioxidant in wheat genotypes, possible mechanism of water stress tolerance. Journal of Argonomy and Grop Science, 184, 55-61
  34. Sakihama, Y & Yamasaki, H. (2002). Lipid peroxidation induces by phenolics in conjunction with aluminium ions. Biology Plantarum, 45, 249- 254.
  35. Sanita, D., Toppi, L. & Gabbrielli, R. (1999). Response to cadmium in higher plants. Environmental and Experimental Botany, 41, 105-130.
  36. Shanker, K. A., Cervantes, C., Loza, H. & Avudainayagam, S. (2004). Chromium toxicity in plant. Environment International, 3, 739-753.
  37. Singleton, V.L., Orthofer, R. & Lamuela-raventos, R. M. (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods in Enzymology, 299, 152-178.
  38. Srivastava, S., Mishara, S., Tripathi, R., Dwivedi, S. & Gupta, D. (2006). Copper induced oxidative stress and responses of antioxidants and phytochelatins in (Hydrilla verticillata). Royle Aquatic Toxicology, 80(4), 405-415.
  39. Sundaramoorthy, P., Alagappan, C., Kaliyaperumal, S. G., Pachikkaran, U. & Logalashmanan, B. (2010). Chromium stress in paddy: (i) Nutrient status of paddy under chromium stress., (ii) Phytoremediation of chromium by aquatic and terrestrial weeds. Comptes Rendus Biologies, 333, 597-607.
  40. Tanentzap, F. M., Stempel, A. & Ryser, P. (2015). Reliability of leaf relative water content (RWC) measurements after storage: consequences for in situ measurements. Botany, 93, 535-541.
  41. Wang, Y., Liu, C., Li, K., Sun, F., Hu, H., Li, X., Zhao, Y., Han, C., Zhang, W., Duan, Y., Liv, M. & Li, X. (2007). Arabidopsis EIN2 modulates stress response through abscisic acid response pathway. Plant Molecular Biology, 64, 633-644.
  42. Warner, R. L. & Kleinhofs, A. (1992). Genetics and molecular biology of nitrate metabolism in higher plants. Physiologia Plantarum, 85(2), 245-252.
  43. Williams, R. J. P. & Frausto Da Silva, J. J. R. (2002). The involvement of molybdenum in life. Biochemical and Biophysical Research Communications, 292(2), 293-299.
  44. Zengin, F. & Munzuroglu, O. (2005). Effect of some heavy metal on content of chlorophyll, proline and some antioxidant chemicals in bean seedling. Acta Biological Cracoviensa Series Botanica, 47, 157-164.