تأثیر متیل جاسمونات و اسید سالیسیلیک در تغییرات آنزیم‌های آنتی‌اکسیدان، بیان ژن ‏bapt‏ و بیوسنتز ‏تاکسان‌ها در گیاهچه‌های حاصل از کشت درون‌شیشه‌ای فندق رقم سگورب ‏‏(‏Corylus avellana L cv. ‘Segorb’‎‏) ‏

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج، ایران

2 استاد، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج، ایران

3 دانشیار، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج، ایران

4 دکتری، مدیر واحد تحقیقات و توسعه شرکت کشت و صنعت رعنا، تهران، ایران

چکیده

تاکسول به­عنوان یک دارو با منشأ گیاهی در درمان سرطان‌های مختلف استفاده می‌گردد. گیاه فندق (Corylus avellana L.) به­عنوان یک منبع جایگزین برای تأمین تاکسول مطرح شده و توجه زیادی را بخود معطوف کرده است. در این مطالعه، تأثیر دو الیسیتور متیل جاسمونات و اسید سالیسیلیک بر روی گیاهچه‌های حاصل از کشت درون­شیشه­ای فندق رقم سگورب مورد بررسی قرار گرفت. فعالیت آنزیم‌های آنتی‌اکسیدانی PAL، کاتالاز، PPO، SOD و نیز محتوی پراکسید هیدورژن مورد مطالعه قرار گرفت. همچنین این تحقیق، با هدف ارزیابی بیان ژن‌های txs، dbat و bapt  بیوسنتز و تولید تاکسان‌ها انجام شد. بررسی میزان فعالیت آنزیم‌های آنتی‌اکسیدانی و محتوی پراکسید هیدروژن حاکی از تأثیر معنی‌دار الیسیتورها در افزایش محتوی H2O2 و افزایش فعالیت آنزیم­های مورد مطالعه در تیمارهای مؤثر بر افزایش متابولیت­ها بود. ژن‌های txs و dbat در فندق رقم سگورب بیان نشدند. بیشترین میزان بیان ژن bapt به­ترتیب در تیمار با اسید سالیسیلیک در غلظت 1/0 میلی­مولار در روز دوم و در تیمار با متیل جاسمونات در غلظت 1/0 میلی­مولار در روز چهارم بود و بالاترین مقدار تاکسان‌ها به­ترتیب در تیمار با متیل جاسمونات 1/0 میلی­مولار و اسید سالیسیلیک 2/0 و 1/0 میلی­مولار به‌دست آمد، به طوری که نسبت به شاهد هشت برابر افزایش نشان داد. در کل، میزان تاکسان­ها از روز دوم تا روز 16 نسبت به شاهد بیشتر بود. بیشترین مقدار 10-deacetyltaxol و تاکسول مربوط به روز چهارم بعد از اعمال الیسیتور بود. در مجموع، هر دو الیسیتور متیل­جاسمونات و اسید سالیسیلیک باعث افزایش بیان نسبی ژنbapt  شدند.

کلیدواژه‌ها


عنوان مقاله [English]

Influence of methyl jasmonate and salicylic acid treatment on the antioxidant ‎enzyme changes, expression of‏ ‏bapt‏ ‏and biosynthesis of taxanes in in vitro plantlets ‎of hazelnut cv ‘Segorb’ ‎

نویسندگان [English]

  • Rahimeh Hemmati Gougeh 1
  • Mohamadreza Fattahi Moghadam 2
  • Majid Shokrpour 3
  • Mohammadreza Naghavi 2
  • Alidad Varshochi 4
1 Ph.D. Candidate, College of Agriculture and Natural Resources, University of Tehran, ‎Karaj, Iran
2 Professor, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
3 Associate Professor, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
4 Ph.D. Head of R&D, RANA Agro-Industry Corporation, Tehran, Iran
چکیده [English]

Taxol is a phytochemical medicine which is used as a chemothrapy medication to treat several cancers. The hazelnut has been suggested as alternative source of taxol and has gained much attention. In this study, the influence of two elicitors, methyl jasmonate and salicylic acid was applied on hazelnut cv. Segorb plantlets originated from in vitro condition. The activation of antioxidant enzymes such as PAL, catalase, PPO, SOD as well as hydrogen peroxide content was studied. Also, this research was carried out intending to evaluate txs, dbat and bapt gene expression, biosynthesis and production of taxanes. The investigations of antioxidant enzyme activities and H2O2 content were indicated the significant effects of such elicitors on increasing H2O2 content and the subsequent rise in catalase activity. txs and dbat genes in hazelnut ‘Segorb’ cultivar did not express. The highest expression of bapt was observed during the second day of treatment with 0.1 mM of SA and 0.1 mM metyljasmonate, while the highest amount of taxanes detected was after treatment with metyljasmonate at 0.1mM, SA at 0.2 and 0.1 mM, receptively, where taxanes detection demonstrated an 8-fold increase compared to the control. Overall, the content of taxanes on the 2 th to 16th day was higher than control. The highest amount of 10-deacetyltaxol and taxol was detected on day 4 after elicitor application. In conclusion, two elicitors metyljasmonate and salicylic acid caused to increase relative gene expression of bapt.

کلیدواژه‌ها [English]

  • Anticancer phytochemical medicine
  • Elicitor
  • gene expression
  • Taxol
  • Tissue culture
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