اثر باکتری‌های تأمین‌کننده نیتروژن و فسفر بر ویژگی‌های نشای گوجه‌فرنگی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشیار، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران

2 دانشجوی دکتری، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران

3 استادیار، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران

چکیده

به منظور بررسی اثر باکتری­های تأمین‌کننده­ی نیتروژن و فسفر بر ویژگی­های نشاء گوجه‌فرنگی، آزمایشی به‌صورت فاکتوریل در قالب طرح کاملا تصادفی با سه تکرار در اسفند و فروردین ماه سال 139۵-۱۳۹۴ در گلخانه دانشگاه علوم کشاورزی و منابع طبیعی گرگان انجام شد. عامل اول، تیمار کود بیولوژیک در پنج سطح (بدون مصرف، آزوسپریلیوم بذرمال، سودوموناس بذرمال، آزوسپریلیوم خاک مصرف، سودوموناس خاک مصرف) و عامل دوم، آبیاری در دو سطح (چهار نوبت در روز و دو نوبت در روز) بود. در این پژوهش از باکتری­های آزوسپریلیوم براسیلنس سویه (of) و سودوموناس فلورسنس سویه (p-169) استفاده شد. نتایج نشان داد استفاده از کودهای بیولوژیک آزوسپریلیوم براسیلنس و سودوموناس فلورسنس به صورت خاک مصرف، توانست نسبت به تیمار بدون مصرف باکتری، بسیاری از خصوصیات مطلوب رشد مانند درصد جوانه­زنی، طول ساقه، وزن خشک اندام­هوایی، وزن تر ریشه، کاهش زمان تا ظهور برگ ششم و افزایش آنتوسیانین بافت (به ترتیب به میزان 67/1، 9/2، 05/19، 25/9، 38/3 و 42/18 درصد نسبت به شاهد) را بهبود دهد. بنابراین می­توان تیمارهای آزوسپریلیوم براسیلنس و سودوموناس فلورسنس خاک مصرف را تحت شرایط رژیم آبیاری معمولی در جهت بهبود کیفیت، کوتاه کردن دوره پرورش، کاهش خسارت تنش آبی و افزایش تحمل به تنش ناشی از انتقال از خزانه به زمین اصلی در تولید نشاء گوجه‌فرنگی توصیه نمود.

کلیدواژه‌ها

عنوان مقاله [English]

The effect of nitrogen and phosphorus supplier bacteria on the characteristics of ‎tomato seedling

نویسندگان [English]

  • Kambiz Mashayekhi 1
  • Ayoub Ghorbani Dehkordi 2
  • Saied Javad Mousavizadeh 3
  • Kamran Rahnama 1
1 Associate Professor, Gorgan University of Agricultural Sciences and Natural Resources, ‎Gorgan, Iran
2 Ph. D. Candidate, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
3 Assistant Professor, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
چکیده [English]

In order to study the effect of nitrogen and phosphorus supplier bacteria on tomato transplants characteristics, a factorial experiment in a completely randomized design with three replications, was conducted in a greenhouse of Gorgan University of Agricultural Sciences and Natural Resources, in March 2016. The first factor was biological fertilizer application at five levels (control, seed treatment of azosperilium, seed treatment of Pseudomonas, Azosperilium soil use, Pseudomonas soil use), and the second factor was irrigation at two levels (four times a day and two times a day). In this experiment, Azospirillium brasilense strain (P) and Pseudomonas fluorescens strain (p-169) were used. The results showed that the use of biofertilizers of Azospirilium brasilense and Pseudomonas fluorescens in soil application compare with control, improved germination percentage, stem length, stem and leave dry weight, root fresh weight, decreased the time to appearance of sixth leaf and increased anthocyanins in the tissue (1.67, 2.9, 19.05, 9.25, 3.38 and 18.42%), respectively. So, Azosperilium brasilense and Pseudomonas fluorescens as soil use can be used in normal irrigation regimes to improve the quality, shorten the nursery period, reduce water stress and improving seedling tolerance to the transmission stress in the main field, in the production of tomato transplants.

کلیدواژه‌ها [English]

  • Anthocyanin
  • antioxidant enzymes
  • Azospirillum
  • Pseudomonas
  • transplant‎

مراجع

  1. Arnon, D. I. (1967). Photosynthesis by isolated chloroplast I.T. central concept and comparison of three prochemical reaction. Biochemical et Biophysica Acta, 20, 440-446.
  2. Azcon, R. & Barea, J.M. (2010). Mycorrhizosphere interactions for legume improvement. In: Khanf, M.S., Zaidi, A. & Musarrat, J., (editors). Microbes for legume improvement. (pp.71-237.). Vienna: Springer.
  3. Beauchamp, C. & Fridovich, I. (1971). Superoxide dismutase: improved assay and an assay applicable to acrylamide gels. Analytical Biochemistry, 44, 276-287.
  4. Blank, L., Cohen, Y., Bomshein, M., Shulhani, R., Sofer, M. & Shtienberg, D. (2015). Variables associated with severity of bacterial canker and Wilt caused by Clavibacter michiganensis michiganensis in tomato greenhouse. Phytopathology, 21(3), 23-32.
  5. Casanovas, E.M., Barassi, C.A., Andrade, F.H. & Sueldo, R.J. (2003). Azospirillum-inoculated maize plant responses to irrigation restraints imposed during flowering. Cereal Research Communications, 31, 395-402.
  6. Chance, B. & Maehly, A.C. (1955). Assay of catalases and peroxidases. Methods in Enzymology, 11, 755-764.
  7. Creus, C.M., Graziano, M., Casanovas, E.M., Pereyra, M.A., Simontacchi, M., Puntarula, S., Barassi, C.A. & Lamattina, L. (2005). Nitric oxide is involved in the Azospirillum brasilense-induced lateral root formation in tomato. Planta, 221, 297-303.
  8. Creus, C.M., Sueldo, R.J. & Barassi, C.A. (2004). Water relations and yield in Azospirillum-inoculated wheat exposed to drought in the field. Canadian Journal of Botany, 82, 273–281.
  9. Ghamari, T., Souri, M.K., Arab, M. & Azadegan B. (2017). Effect of different levels and methods of phosphorus fertilizer application on growth and development of two stock (Matthiola incana) Cultivars. Iranian Journal of Plant Production Technology, 9(2), 1-13.
  10. Ghorbani Dehkordi, A., Mashayekhi, K. & Kamkar, B. (2015a). Effect of foliar application sucrose, boron, potassium nitrate and salicylic acid on yield and yield components of tomato var. Super A. Research in Crop Ecosystems, 2(1), 43-52. (in Farsi)
  11. Ghorbani Dehkordi, A., Mashayekhi, K., Kamkar, B. & Rahmani, B. (2015b). Effect of foliar application of salicylic acid and lime sulfur on some quantitative and qualitative characteristics of tomato transplanting var. Super A. Applied Research of Plant Ecophysiology, 2(1), 65-80. (in Farsi).
  12. Hasandokht M.R. (2012). Technology of vegetable production. Selseleh Publications, Qom, Iran, pp.558. (in Farsi).
  13. Hatami, M., Khanizadeh, P., Alsadat Abtahi, F. & Abaszadeh Dehgi, P. (2019). Influence of plant growth promoting rhizobacteria and hydro-priming on some physiological indices of lemon balm (Melissa officinalis). Iranian Journal of Horticultural Science, 52 (1), 11-21. (in Farsi).
  14. Jaime, P. & Fernande, N. (2008). Handbook of plant breeding “Vegetables II”. Springer, Valencia, Spain.
  15. Javanmardi, J. (2009). Scientific and applied basis for vegetable transplant production. Jahad Daneshgahi Press, Mashhad. (in Farsi)
  16. Kamoshita, A., Babu, R.C., Boopathi, N.M. & Fukai, S. (2008). Phenotypic and genotypic analysis of drought-resistance traits for development of rice cultivars adapted to rainfed environments. Field Crops Research, 109, 1-23.
  17. Kar, M. & Mishra, D. (1976). Catalase, peroxidase and polyphenoloxidase activities during rice leaf senescence. Plant Physiology, 57, 315-319.
  18. Kimbrel, J.A., Givan, S.A., Halgren, A.B., Creason, A.L., Mills, D.I., Banowetz, G.M., Armstrong, D.J. & Chang, J.H. (2010). An improved, high-quality draft genome sequence of the germination arrest factor-producing Pseudomonas fluorescens BMC Genomics, 11, 522.
  19. Kucey, R.M.N., Janzen, H.H. & Legett, M.E. (1989). Microbially mediated increase in plant available phosphorus. Advances in Agronomy, 42, 199-228.
  20. Mascher, F., Hase, C., Moenne-Loccoz, Y. & Defago, G. (2000). The viable-but-nonculturable state induced by abiotic stress in the biocontrol agent Pseudomonas fluorescens CHA0 does not promote strain persistence in soil. Applied Environment Microbiology, 66, 1662–1667.
  21. Motsara, M.R., Bhattacharyya, P. & Srivastava, B. (1995). Biofertiliser technology, marketing and usage. A Sourcebook Cum Glossary, pp.194.
  22. Naiji, M. & Souri, M. K. (2015). Evaluation of growth and yield of savory (Satureja hortensis) under organic and biological fertilizers toward organic production. Journal of Plant Productions,38(3), 93-103. (in Farsi)
  23. Agronomy, Breeding and Horticulture (Scientific Journal of Agriculture) 38(3), 93-103. (in Farsi)
  24. Naiji, M. & Souri, M. K. (2018). Nutritional value and mineral concentrations of sweet basil under organic compared to chemical fertilization. Acta Scientiarum Polonorum Hortorum Cultus, 17(2), 167-175.
  25. Okon Y. & Labandera-Gonza´lez, C.A. (1994). Agronomic applications of Azospirillum: an evaluation of 20 years world wide field inoculation. Soil Biology and Biochemistry, 26, 1551–1601.
  26. Pourranjbari Saghaiesh, S., Souri, M.K. & Moghaddam, M. (2019). Characterization of nutrients uptake and enzymes activity in Khatouni melon (Cucumis melo inodorus) seedlings under different concentrations of nitrogen, potassium and phosphorus of nutrient solution. Journal of Plant Nutrition, 42(2), 178-185.
  27. Ramamoorthy, V., Raguchander, t. & Samiyappan, R. (2002). Induction of defense-related proteins in tomato roots treated with Pseudomonas fluorescens Pf1 and Fusarium oxysporum sp. lycopersici. Plant and Soil, 239, 55-68.
  28. Rodriguez, H. & Fraga, R. (1999). Phosphate solubilizing bacteria and their role in plant growth promotion. Biotechnology Advances, 17, 319-339.
  29. Ruiz-Sanchez, M., Armada, E., Munoz, Y., Garcia de Salamone, Aroca, R., Ruiz-Lozano, J.M. & Azcon, R. (2011). Azospirillum and arbuscular mycorrhizal colonization enhance rice growth and physiological traits under well-watered and drought conditions. Plant Physiology, 168, 1031-1037.
  30. Saravanakumar, D. & Samiyappan, R. (2007). ACC deaminase from Pseudomonas fluorescens mediated saline resistance in groundnut (Arachis hypogea) plants. Applied Microbiology, 102, 1283-1292.
  31. Schilling, G., Gransee, A., Deubel, A., Lezovic, G. & Ruppel, S. (1998). Phosphorus availability, root exudates and microbial activity in the rhizosphere. Pflanzenerna'hr. Bodenk, 161, 465–478.
  32. Shekari, F., Massiha, S. & Esmailpoor, B. (2005). The physiology of vegetable crops. Zanjan University Press, pp.394. (in Farsi)
  33. Shekhalipour, M., Bolandnazar, S.A. Sarikhani, M.R. & Panahandeh, J. (2018). Effect of application of biofertilizers on yield, quality and antioxidant capacity of tomato fruit. Iranian Journal of Horticultural Science, 50 (3), 621-632. (in Farsi)
  34. Somers, E., Ptacek, D., Gysegom, P., Srinivasan, M. & Vandeleyden J. (2005). Azospirillum brasilense produces the auxin-like phenylacetic acid by using the key enzyme for indole-3-acetic acid biosynthesis. Applied and Environmental Microbiology, 71(4), 1803-1810.
  35. Souri, M. K. & Yaghoubi Sooraki, F. (2019). Benefits of organic fertilizers spray on growth quality of chili pepper seedlings under cool temperature. Journal of Plant Nutrition, 42 (6), 650-656.
  36. Wanger, G.J. (1979). Content and vacuole/extra vacuole distribution of neutral sugars, free amino acids, and anthocyanins in protoplasts. Journal of Plant Physiology, 64, 88-93.
  37. Xu, Y.C., Zhang, J.B., Jiang, Q.A., Zhou, L.Y. & Miao, H.B. (2006). Effects of water stress on the growth of Lonicera japonica and quality of honeysuckle. Zhong Yao Cai., 29(5), 420-423.
  38. Yue, H.T., Mo, W.P. & Li, C. (2007). The salt stress relief and growth promotion effect of Rs-5 on cotton. Plant Soil, 297, 139-145.
  39. Zekavati, H.R., Mansoori, N. & Fatemi, S.R. (2019). Effect of Trichoderma harzianum fungus on the physiology traits and some vegetative and reproductive characteristics of tuberose (Polianthes tuberose cv. Double) under drought stress conditions. Iranian Journal of Horticultural Science, 51 (4), 1017-1026. (in Farsi)
علوم باغبانی ایران
دوره 52، شماره 1
خرداد 1400
صفحه 113-123

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سابقه مقاله

  • تاریخ دریافت: 27 خرداد 1398
  • تاریخ بازنگری: 03 آذر 1398
  • تاریخ پذیرش: 06 آذر 1398

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