تأثیر باکتری‌های محرک رشد گیاه و هیدروپرایمینگ بر برخی شاخص‌های فیزیولوژیکی گیاه دارویی ‏بادرنجبویه (‏Melissa officinalis‏)‏

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشیار، دانشکده کشاورزی و منابع طبیعی، دانشگاه اراک، اراک، ایران

2 دانش‌آموخته کارشناسی ارشد، دانشکده کشاورزی و منابع طبیعی، دانشگاه اراک، اراک، ایران

3 استادیار، دانشکده کشاورزی و منابع طبیعی، دانشگاه اراک، اراک، ایران

4 استادیار، دانشکده کشاورزی، دانشگاه ولیعصر(عج) رفسنجان، رفسنجان، ایران

چکیده

کاربرد باکتریهای محرک رشد موجب تحریک متابولیسم و فرایند‌های متابولیکی در جهت افزایش کارایی گیاهان می-شوند. این مطالعه با هدف بررسی اثر هیدروپرایمینگ بذر و تلقیح باکتری‌های محرک رشد سویه‌های فلورسنس و پوتیدا به خاک به عنوان محرک زیستی بر برخی صفات فیزیولوژیکی گیاه دارویی بادرنجبویه (Melissa officinalis) انجام شد. این آزمایش به صورت فاکتوریل در قالب طرح کاملا تصادفی در شرایط گلخانه و آزمایشگاه گروه گیاهان دارویی دانشگاه اراک انجام شد. فاکتور اول هیدروپرایمینگ در زمان‌های مختلف (12، 24، 48 و 72 ساعت)، و فاکتور دوم دو سویه باکتری (فلورسنس و پوتیدا)، شاهد (بدون تلقیح) بود. نتایج نشان داد که هیدروپرایمینگ بذر در زمان‌های مختلف و باکتری‌های محرک رشد موجب افزایش رنگیزه-های فتوسنتزی، فنل، فلاونوئیدکل، درصد و عملکرد اسانس شد. همچنین هیدروپرایمینگ به مدت 48 ساعت به همراه باکتری پوتیدا، منجر به افزایش چشمگیر میزان فلاونوئید شد و در مدت 12 ساعت به همراه باکتری فلورسنس اثر معنی داری بر مقدار فنل داشت. با توجه به نتایج مثبت و افزایشی باکتری‌ها بر شاخص‌های مورد بررسی گیاه دارویی بادرنجبویه، می‌توان گفت باکتری‌های بکار رفته در این تحقیق می‌توانند به‌عنوان یک جایگزین مناسب برای کود شیمیایی ضمن کاهش مصرف کودهای شیمیایی، تضمین‌کننده تولید گیاهان دارویی عاری از مواد شیمیایی باشند.

کلیدواژه‌ها

عنوان مقاله [English]

Influence of plant growth promoting rhizobacteria and hydro-priming on some ‎physiological indices of lemon balm (Melissa officinalis)‎

نویسندگان [English]

  • Mehrnaz Hatami 1
  • Parisa Khanizadeh 2
  • Faezeh alsadat Abtahi 3
  • Peyman Abaszadeh Dehgi 4
1 Associate Professor, Faculty of Agriculture and Natural Resources, Arak University, ‎Arak, Iran
2 Former M.Sc. Student, Faculty of Agriculture and Natural Resources, Arak University, Arak, Iran
3 Assistant Professor, Faculty of Agriculture and Natural Resources, Arak University, Arak, Iran
4 Assistant Professor, Faculty of Agriculture, University of Vali-e-Asr, Rafsanjan, Rafsanjan, Iran
چکیده [English]

Application of plant growth promoting rhizobacteria metabolism and metabolic processes to increase plant efficacy. The aim of this study was to investigate the effect of seeds hydropriming and inoculation of plant growth promoting rhizobacteria (Pseudomonas fluoresense, Pseudomonas p < /em>utida) to soil as biological stimulative on some physiological traits of Melissa officinalis. The experiment was conducted as a factorial based on a completely randomized design in greenhouse conditions and laboratory of Medicinal Plants Department of Arak University. The first factor was hydropriming at different times (12, 24, 48 and 72 hours), and the second factor was two bacterial species (Pseudomonas fluoresense, Pseudomonas putida), control (without inoculation). The results showed that seed hydropriming at different times and growth promoters increased the photosynthetic pigments, phenol, flavonoids, percentage and essential oil yield. Also, hydropriming for 48 hours with Pseudomonas putida bacteria resulted in a significant increase in flavonoid content and a significant effect on phenol content during 12 hours with Pseudomonas fluoresensebacteria. According to the positive and additive effects of plant growth promoting rhizobacteria on Melissa officinalis, the plant growth promoting rhizobacteria could be recommended as an alternative to chemical fertilizers to reduce the consumption of chemical fertilizers and guaranty the production of medicinal plants, free of chemicals.

کلیدواژه‌ها [English]

  • Essential oil
  • flavonoid
  • photosynthetic pigments
  • priming
  • rhizobacteria

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علوم باغبانی ایران
دوره 52، شماره 1
خرداد 1400
صفحه 11-21

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سابقه مقاله

  • تاریخ دریافت: 15 اردیبهشت 1398
  • تاریخ بازنگری: 31 خرداد 1398
  • تاریخ پذیرش: 14 مرداد 1398

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