اثر سالیسیلیک‌اسید‌ و متیل‎ ‎‏‌جاسمونات بر تولید فنل، فلاونوئید و رنگدانه‌های کالوس گیاه پروانش ‏‏(‏Catharanthus roseus L.‎‏)‏

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانش‌آموخته کارشناسی ارشد، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری، ایران

2 دانشیار، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری، ایران

چکیده

محرک­های غیر زیستی سالیسیلیک‌اسید (SA) و متیل جاسمونات (MJ) می‌توانند سبب افزایش تولید متابولیت­های ثانویه گیاهان شوند. در این پژوهش، اثر SA و MJ بر وزن تر، میزان فلاونوئید، فنل کل و رنگدانه­های کالوس پروانش در دو آزمایش فاکتوریل در قالب طرح کاملاً تصادفی مطالعه شد. فاکتور اول در هر آزمایش غلظت­ محرک غیر­زیستی SA یا MJ در چهار سطح صفر، 50، 100 و 200 میکرومولار و فاکتور دوم، مدت زمان استفاده از غلظت­های مختلف محرک­ها در دو سطح  5 و 10 روز بود که هر دو آزمایش با سه تکرار انجام شدند. بر اساس نتایج اثر متقابل زمان و SA بر کلیه صفات، به­غیر از وزن تر کالوس و آنتوسیانین، معنی‌دار شد و اثر متقابل زمان و MJ بر میزان فنل کل، کلروفیل و کاروتنوئید معنی‌دار گردید (01/0P<). بیشترین میزان فنل کل (123/55 میلی­گرم در گرم وزن تر) مربوط به تیمار 100 میکرو­مولار MJ در 5 روز بود و بیشترین میزان فلاونوئید (467/21 میلی­گرم در گرم وزن تر) در تیمار 50 میکرو­مولار SA در 5 روز به‌دست آمد. بنابراین، غلظت­های 50 و 100 میکرومولار محرک­های غیر­زیستی SA و MJ در 5 روز می‌توانند برای افزایش میزان فلاونوئید و فنل کل کالوس پروانش مورد استفاده قرار گیرند.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of salicylic acid and methyl jasmonate on production of phenol, flavonoid and ‎pigments of Madagascar periwinkle (Catharanthus roseus L.) callus

نویسندگان [English]

  • Atiyeh Niyazazari 1
  • Vida Chalavi 2
  • Sara Kabirnataj 1
1 M. Sc. Graduate, Sari Agricultural Sciences and Natural Resources University, Sari, Iran
2 Associate Profesosr, Sari Agricultural Sciences and Natural Resources University, Sari, Iran
چکیده [English]

Salicylic acid (SA) and methyl jasmonate(MJ) are abiotic elicitors which can increase production of secondary metabolites in plants. Effects of SA and MJ on fresh weight, flavonoids, total phenol and pigments of Madagascar periwinkle callus were investigated in two factorial experiments, in a completely randomized design. The first factor was the concentration of a different abiotic elicitor, SA or MJ in 4 levels of 0, 50, 100 and 200 μM, and the second factor was time of using different concentrations of elicitors at 2 levels of 5 and 10 days, with three replications. Based on results, the interaction between time and SA in all traits, unless fresh weigh of callus and production of anthocyanin, was significant and the interaction between time and MJ on total phenol, chlorophyll and carotenoids production was significant (P<0.01). The highest total phenol content (55.123 mg/g F.W.) was obtained from 100 μM MJ treatment for 5 days and the highest amount of flavonoid (21.467 mg/g F.W.) was obtained in 50 μM SA treatment for 5 days. Therefore, 50 and 100 μM concentrations of SA and MJ abiotic elicitors in 5 days can be used for increase the production of flavonoid and total phenol in catharanthus roseus L. callus.

کلیدواژه‌ها [English]

  • Anthocyanin
  • Carotenoids
  • ‎‏ ‏chlorophyll
  • Medicinal plant
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