تأثیر محلول‌پاشی نانو اکسید آهن و کودهای زیستی بر فعالیت آنزیم‌های آنتی اکسیدان و برخی ‏خصوصیات فیزیولوژیکی ریشه و بخش هوایی سیاه‌دانه (‏Nigella sativa L.‎‏)‏

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری، دانشکده کشاورزی، دانشگاه صنعتی شاهرود، شاهرود، ایران

2 دانشیار، دانشکده کشاورزی، دانشگاه صنعتی شاهرود، شاهرود، ایران

10.22059/ijhs.2019.274442.1591

چکیده

به منظور بررسی تأثیر نانو اکسید آهن و انواع مختلف کودهای زیستی بر رشد ریشه، فعالیت آنزیم‌های آنتی‌اکسیدان در در دو بخش ریشه و برگ و نیز برخی دیگر از خصوصیات فیزیولوژیکی سیاه‌دانه، آزمایشی گلدانی به‌صورت فاکتوریل بر پایه طرح بلوک­های کامل تصادفی با سه تکرار در سال 1396 در دانشگاه صنعتی شاهرود اجرا شد. عامل اول محلول­پاشی نانو اکسید آهن در سه سطح (صفرF0=، 5/1F1= و 3 F2= گرم در لیتر آب) و عامل دوم کود­ زیستی در پنج سطح (عدم مصرف کود زیستی (شاهد)B0Glomus intraradicesB1=، B2= Glomus mosseae، آزوسپریلیوم B3= و ازتوباکتر B4=) بودند.  نتایج نشان داد فعالیت آنزیم‌های آنتی‌اکسیدانی کاتالاز، پلی فنل اکسیداز در دو بخش ریشه و برگ تحت تأثیر دو عامل کودهای زیستی و محلول­پاشی نانو اکسید آهن قرار گرفت، به‌طوری‌که استفاده از کودهای زیستی سبب کاهش و با افزایش غلظت نانو اکسید آهن، فعالیت آنها افزایش یافت. بیشترین میزان فعالیت آنزیم آسکوربات پراکسیداز در برگ نیز از تیمارF2B0  حاصل شد. اثر متقابل محلول‌پاشی نانو اکسید آهن و کودهای زیستی بر رنگدانه های فتوسنتزی و وزن خشک اندام هوایی معنی‌دار بود، به‌طوری‌که بیشترین مقدار کلروفیل a از تیمار F2B1، کلروفیل b، وزن خشک اندام هوایی و آهن اندام هوایی از تیمار F2B2 حاصل شدند. با توجه به نتایج آزمایش همزیستی با قارچ G. intrarardices موجب افزایش معنی‌دار جذب عناصر نیتروزن و آهن در گیاه شد.

کلیدواژه‌ها


عنوان مقاله [English]

Effects of foliar application of nano iron oxide and biofertilizers on the activity of ‎antioxidant enzymes and some physiological characteristics of the root and aerial ‎parts in black cumin (Nigella sativa L.)‎

نویسندگان [English]

  • Maryam Bromand Sivieri 1
  • Mostafa Heidary 2
  • Ahmad Gholami 2
  • Hadi Ghorbani 2
1 Ph.D. Candidate, Faculty of Agriculture, Shahrood University of Technology, Shahrood, Iran‎
2 Associate Professor, Faculty of Agriculture, Shahrood University of Technology, Shahrood, Iran‎
چکیده [English]

In order to study the effects of foliar application of nano iron oxide particles and biofertilizers on root growth, antioxidant enzymes activity in two parts of root and leaf, as well as some other physiological characteristics of black cumin (Nigella sativa L.) a pot experiment with factorial arrangement in randomized complete block design with three replications was carried out in 2017 growing season at the Faculty of Agriculture, University of Shahrood. The first factor was foliar applications of nano iron oxide at three levels (F0= control, F1= 1.5 and F2= 3 g /1 l water) and the second factor was biofertilizers at five levels (B0= Control, B1= Glomus Intraradices, B2= Glomus mosseae, B3= Azospirillum and B4= Azotobacter solution). The results showed that activity of antioxidant enzymes catalase, polyphenol oxidase in two parts of root and leaf was influenced by two factors of biological fertilizers and foliar application of nanoiron oxide particles, so that the use of biofertilizers reduced and with increasing iron oxide concentration, activity they increased. The highest activity of ascorbate peroxidase in leaf was obtained from F2B0 treatment. The interaction effect of foliar application of nanoiron oxide particles and biofertilizers had a significant effect on photosynthetic pigments and aerial dry weight, so that the highest amount of chlorophyll a was obtained from F2B1 treatment, chlorophyll b, aerial dry weight and iron of aerial from F2B2 treatment. According to the results of the experiment, the symbiosis with G. intrarardits fungus caused a significant increase in the absorption of nitrogen and iron elements in the plant.

کلیدواژه‌ها [English]

  • Ascorbate peroxidase
  • Catalase
  • Chlorophyll
  • Mycorrhiza
  • polyphenol oxidase‎
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