شناسایی آلل‌های خودناسازگاری برخی از ژنوتیپ‌های بادام استان یزد با استفاده از روش ‏PCR

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد، دانشکده کشاورزی، دانشگاه زابل، زابل، ایران

2 دانشیار، دانشکده کشاورزی و منابع طبیعی، دانشگاه یزد، یزد، ایران

3 دانشیار، دانشکده کشاورزی، دانشگاه زابل، زابل، ایران

4 دانشیار، پژوهشکده میوه‌های معتدله و سردسیری، موسسه تحقیقات علوم باغبانی، سازمان تحقیقات، آموزش و ترویج ‏کشاورزی، کرج، ایران

چکیده

یکی از مشکلات تولید بادام، خودناسازگاری در این گیاه بوده که از نکات مهم اصلاحی این گیاه تلقی می‌شود. خودناسازگاری در بادام باعث غیر یکنواختی زمان برداشت، مشکلات مربوط به گرده‌افشانی و بروز مشکلاتی در مدیریت باغ خواهد شد. اغلب ارقام بادام دارای خودناسازگاری از نوع گامتوفتیکی هستند که توسط یک مکان ژنی چند آللی کنترل می‌شود. عامل بازدارنده لقاح گل در این پدیده توقف رشد لوله گرده در میانه خامه می­باشد. هدف از این تحقیق شناسایی و تعیین آلل­های مربوط به خودناسازگاری در ژنوتیپ‌های بادام در استان یزد بوده است. در این تحقیق، ژنوتیپ‌های برتر بادام جمع­آوری و پس از استخراجDNA  برای تشخیص آلل­های S جفت آغازگرهای اختصاصی AS1II-AmyC5R،ConF-ConR  و Cebador2-Cebador8 مورد استفاده قرار گرفت. در واکنش زنجیره­ای پلیمراز جفت آغازگر AS1II-AmyC5R و Cebador2-Cebador8 به ترتیب باندهایی به اندازه 1205 و 1200 جفت بازی برای آلل Sf تشکیل گردید. لازم به ذکر است ارقام خودسازگار از تلاقی تونو با ترکیب آللی S1Sfبه‌عنوان والد پدری و خودسازگار و آیدین با ترکیب آللی S1S23به‌عنوان والد مادری و خودناسازگار به‌دست‌آمده است. با استفاده از جفت آغازگر ConF-ConR آلل­های S1، S2، S3، S10، S11، S23 و S31در نمونه­های خودناسازگار شناسایی شدند. در این تحقیق آلل­های­ Sf، S3، S2، S1، S5، S10،S11، S23 و S13با استفاده از جفت آغازگر AS1II-AmyC5R شناسایی شدند. بر اساس نتایج به‌دست‌آمده  آلل­های S1، S2، S3 و S11  دارای بیشترین فراوانی و آلل‌های S5، S23، S10، S13وS31 به ترتیب کمترین درصد فراوانی را نشان دادند.

کلیدواژه‌ها


عنوان مقاله [English]

Identification of self-incompatibility alleles in some of Yazd province almond ‎genotypes by using PCR method‏ ‏

نویسندگان [English]

  • Mitra Abouei 1
  • Kazem Kamali Aliabad 2
  • Mahmood Soluki 3
  • leila Fahmideh 3
  • Ali Imani 4
1 M.Sc. Student, Faculty of Agriculture, Zabol University, Zabol, Iran
2 Associate Professor, Faculty of Agriculture and Natural Resources, Yazd University, Yazd, Iran
3 Associate Professor, Faculty of Agriculture, Zabol University, Zabol, Iran
4 Associate Professor, Temperate Fruits Research Center, Horticultural Science Research Institute(HSRI), Agricultural Research, ‎Education and Extension Organization (AREEO), Karaj, Iran
چکیده [English]

One of the problems in almond production is self-incompatibility in this plant, which is considered as an important point of breeding. Self-incompatibility causes non-uniformity of harvesting time as well as some of garden management and pollination problems. Most cultivars of almonds have gametophytic self-incompatibility that is controlled by a multi-allelic gene locus. The fertilization inhibitor factor in this phenomenon, pollen tube growth stops in the middle of the style. The purpose of this research was identification and determination of the self-compatible genotype in the Yazd province.in this investigation better genotypes of almond were collected and after DNA extraction was done, in order to detect S alleles in different almond and some hybrid genotypes, the specific primer pairs, including AS1II-AmyC5R, ConF-ConR and Cebador2-Cebador8, were used in the polymerase chain reaction. In polymerase chain reaction, using the AS1II-AmyC5R and Cebador2-Cebador8 primers, the Sf allele with the size of 1200 base pairs was detected. Using the ConF-ConR pair of primer, the S1, S2, S3, S10, S11, S23, and S31 alleles were detected in the self-incompatible samples. Using AS1II-AmyC5R pair of primer, the known alleles of S3, Sf, S2, S1, S5, S10 S11 S23, and S13 were detected. The other bands obtained from the PCR were related to the known self-in-compatibility alleles that might be considered as new alleles.  According to the obtained results, S1, S2, S3, and S11alleles had the highest frequency and S5, S23, S10, S13and S31alleles respectively had the lowest frequency.

کلیدواژه‌ها [English]

  • almond
  • Molecular marker
  • PCR
  • S-allele
  • Self-compatibility
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