اثر سولفات منگنز بر تحمل تنش کم آبی دو رقم انگور در شرایط درون‌شیشه‌ای

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری، دانشکده کشاورزی، دانشگاه شیراز، شیراز، ایران

2 استاد، دانشکده کشاورزی، دانشگاه شیراز، شیراز، ایران

3 دانشیار، دانشکده کشاورزی، دانشگاه بوعلی سینا، همدان، ایران

4 دانشیار، دانشکده کشاورزی، دانشگاه شیراز، شیراز، ایران

5 استادیار، دانشکده کشاورزی، دانشگاه شیراز، شیراز، ایران

چکیده

خشکی از مهمترین تنش‌های غیرزنده است که روی رشد و جنبه‌های فیزیولوژیکی انگور اثر می‌گذارد. در این پژوهش اثر سولفات منگنز بر صفات مورفو-فیزیولوژیک و فعالیت آنزیم سوپراکسیددیسموتاز دو رقم انگور تامسون‌سیدلس و رطبی تحت تنش کم‌آبی در شرایط درون‌شیشه‌ای ارزیابی شد. آزمایش به‌صورت فاکتوریل در قالب طرح کاملاً تصادفی با 8 تکرار انجام شد. از محیط موراشیگ و اسکوگ (MS) با سه تیمار بدون منگنز (صفر میلی‌گرم در لیتر)، 9/16 میلی‌گرم در لیتر (غلظت استاندارد) و 8/33 میلی‌گرم در لیتر (دو برابر غلظت استاندارد) و تیمار تنش کم‌آبی در 4 سطح توسط مقادیر صقر، 3، 9 و 12 درصد وزنی به حجمی پلی‌اتیلن‌گلایکول 6000 برای هر دو رقم انگور استفاده شد. مریستم‌های نوک شاخه به‌عنوان ریز‌نمونه‌های گیاهی استفاده شدند. صقات رشدی شامل ارتفاع گیاه، وزن‌خشک ساقه، سطح و تعداد برگ و محتوای نسبی آب‌برگ تحت تأثیر تنش کم‌آبی کاهش یافت، اما تیمار سولفات منگنز باعث افزایش قابل توجهی در این صفات شد. بیشترین فعالیت آنزیم‌ سوپراکسیددیسموتاز برگ در تیمار 8/33 میلی‌گرم در لیتر سولفات منگنز در سطح تنش 12 درصد پلی‌اتیلن‌گلایکول در رقم رطبی (Umin-1g-1FW 4/65) دیده شد. نتایج این پژوهش پیشنهاد ‌می‌کند که سولفات منگنز می‌تواند موجب افزایش تحمل به تنش کم‌آبی در دو رقم انگور رطبی و تامسون سیدلس در شرایط درون‌شیشه‌ای شود.

کلیدواژه‌ها


عنوان مقاله [English]

The effect of MnSO4 on water stress tolerance in two cultivars of grapevine ‎‎(Vitis vinifera cv. L.) under in vitro condition

نویسندگان [English]

  • Parastoo Ghorbani 1
  • Saied Eshghi 2
  • Ahmad Ershadi 3
  • Akhtar Shekafandeh 4
  • Fatemeh Razaghi 5
1 Ph.D. Candidate, Faculty of Agriculture, Shiraz University, Shiraz, Iran
2 Professor, Faculty of Agriculture, Shiraz University, Shiraz, Iran
3 Asociated Professor, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran
4 Associate Professor, Faculty of Agriculture, Shiraz University, Shiraz, Iran
5 Assistant Professor, Faculty of Agriculture, Shiraz University, Shiraz, Iran
چکیده [English]

Drought is one of the most important abiotic stresses that have effect on growth and physiological aspect of grape. In this research effect of manganese sulfate on the morpho-physiological traits and superoxide dismutase enzyme activity in Thompson seedless and Rotabi grape cultivars under invitro at drought stress was evaluated. The manganese sulfate treatment was carried out across three levels, including MS without manganese (0 mg/L), MS with a standard manganese concentration (16.9 mg/L) and MS with twice the standard manganese concentration (33.8 mg/L), and the drought stress treatment was performed across four levels using 0, 3, 9 and 12% (w/v) solutions of polyethylene glycol (PEG) 6000 in two grape cultivars, namely seedless Thompson and Rotabi. The meristems of shoottip were used as explants. All growth parameters, including plant height, stem dry weight, leaf area, leaf number and relative water content (RWC) were reduced under the impact of drought. However, manganese sulfate treatment caused a significant increase in all these parameters at all concentrations. The highest amounts of superoxide dismutase enzyme activity was observed in 33.8 mg/L MnSO4 under drought stress with 12% PEG in the Rotabi cultivar (65.4 Umin-1g-1FW). The results of this research suggest that manganese sulfate treatment under invitro condition can improve water stress tolerance in both grapevines seedless Thompson and Rotabi cultivars.

کلیدواژه‌ها [English]

  • Meristems
  • polyethylene glycol
  • Plant height
  • SOD‎
Ahmadi, K., Gholizadeh, H., Ebadzadeh, H. R., Hatami, F., Hoseinpur, R., Kazemifard, R. & Abdeshah, H. (2016). Statistics Agriculture. Horticulture Crops. (in Farsi). https://horticulture.maj.ir
Anjum, S. A., Xie, X., Wang, L. C., Saleem, M. F., Man, C. & Lei, W. (2011). Morphological, physiological, and biochemical responses of plants to drought stress. African Journal of Agricultural Research, 6, 2026-2032.
Bates, L. S. & Waklren, R. P. (1973). Rapid determination of free proline water stress studies. Plant Soil, 39, 205-207.
Batukaev, A. A., Magomadov, A. S. & Malyh, G. P. (2014). Influence of manganese fertilizer on efficiency of grapes on sandy soils of the Chechen Republic. In: BIO Web of Conferences (pp.01007). EDP Sciences.
Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Analytical Biochemistry, 72, 248-254.
Dhindsa, R. S. & Matowe, W. (1981). Drought tolerance in two mosses: correlated with enzymatic defence against lipid peroxidation. Journal of Experimental Botany, 32, 79–91.
Doulati Baneh, H. (2016). Salinity effects on plant tissue nutritional status as well as growth and physiological factors in some cultivars and interspecies hybrids of grape. Iranian Journal of Horticultural Sciences, 47(1), 33-44. (In Farsi)
Ghaderi, N. (2009). The Effect of Water Stress on Some Physiological Properties of Five Grape Cultivars and Evaluation of its Genetic Diversity in Kurdistan. Ph.D. Thesis, University of Tehran. 150 p.
Gill. S. S., Anjum, N. A., Gill, R., Yadav, S., Hasanuzzaman, M. & Fujita, M. (2015). Superoxide dismutase-mentor of abiotic stress tolerance in crop plants. Environmental Science and Pollution Research, 22, 10375–10394.
Gul, H., Seema, A., Shabeena, H., Aziz, L., Rahim, Z., Sahar, S. & Pervez1, N. (2017). Exogenous application of zinc and manganese for improve chemical constituents in Brassica Juncea under drought stress. Journal Application Environmental Biology Science, 7, 81-90.
Hasanuzzaman, M., Hossain, M. A., Teixeira Dasilva, J. A. & Fujita, M. (2012). Plant responses and tolerance to abiotic oxidative stress. In: V. Bandi., Shanker, A. K., Shanker, C. & Mandapaka M. (Eds.), antioxidant defense is a key factor: Crop stress and its management. (PP. 261-316). Springer Berlin.
Hadadinejad, M., Ebadi, A., Fatahi, R., Mousavi, A., Santesteban, L. G. & Nejatianc, M. A. (2014). The effect of drought stress on photosynthetic traits and the expression of some genes for a few Iranian grapevine candidate rootstocks. Acta Horticulturae, 1045, 133-138.
Heath, R. L. & Packer, L. (1965). Effect of light on lipid peroxidation in chloroplasts. Biochemical and Biophysical Research Communications, 19, 716-720.
Hicox, J. D. & Israelstam, G. F. (1979). A method for the extraction of chlorophyll from leaf tissue without maceration. Canadian Journal of Botany, 57, 1332-1334.
Lisar, S. Y. S., Motafakkerazad, R., Hossain, M. M. & Rahman, I. M. M. (2012). Water stress in plants. In: M. Rahman & H. Hasegawa, (Eds.), causes, effects and responses. (PP. 1-14). Croatia.
Lovisolo, C., Perrone, I., Carra, A., Ferrandino, A., Flexas, J., Medrano, H. & Schubert, A. (2010). Drought-induced changes in development and function of grapevine (Vitis spp.) organs and in their hydraulic and non-hydraulic interactions at the whole-plant level: a physiological and molecular update. Functional Plant Biology, 37(2), 98-116.
Lutts, S., Kinet, J. M. & Bouharmont, J. (1995). Changes in plant response to NaCl during development of rice verities differing in salinity resistance. Journal of Experimental Botany, 46, 1843-1852.
Lidon, F. C., Barreiro, M. & Ramalho, J. (2004). Manganese accumulation in Rice: implications for photosynthetic functioning. Journal of Plant Physiology, 161, 1235–1244.
Kantar, M., Lucas, S. J. & Budak, H. (2011). Drought Stress: Molecular Genetics and Genomics Approaches. Faculty of Engineering and Natural Sciences, Sabanci University, Istanbul, Turkey. Advances in Botanical Research.
Kardavani, P. (1994). Arid areas. (9th ed.). Tehran University Press. (in Farsi).
Kobraee, S. & Shamsi, K. (2013). Impact of micronutrients foliar application on soybean yield and its components under water deficit condition. Journal of Biodiversity and Environmental Sciences, 3, 39-45.
Marschner, H. (1995). Mineral Nutrition of Higher Plants. Academic Press London.
Millaleo, R., reyes-diaz, M., Ivanov, A. G., Mora, M. L. & Alberdi, M. (2010). Manganese as essential and toxic element for plants: transport, accumulation and resistance mechanisms. Journal of Soil Science Plant Nutrition, 10, 476-494.
Mousavi, S. R., Shahsavari, M. & Rezaie, M. (2011). A general overview on Manganese (Mn) importance for crops production. Australian Journal of Basic and Applied Sciences, 5(9), 1799-1803.
Movahedy-dehnavy, M., Modarressanavy, S. A. M. & Mokhtassi-bidgoli, A. (2009). Foliar application of zinc and manganese improves seed yield and quality of Safflower (Carthamus tinctorius L.) grown under water deficit stress. Industrial Crops and Products, 30, 82-99.
Malakouti, M. J., Keshavarz, P. & Karimian. (2008). A comprehensive approach towards identification of nutrients deficiencies and optimal fertilization for sustainable agriculture. Tarbiat Modares University Press. (in Farsi)
Munns, R. (2011). Plant adaptations to salt and water stress: differences and commonalities. Advances in Botanical Research, 57, 1-32.
Pradubsuk, S. & Davenport, J. R. (2011). Seasonal distribution of micronutrients in mature ‘Concord’ grape: boron, iron, manganese, copper, and zinc. Journal of the American Society for Horticultural Science, 136(1), 69-77.
Rabiei, V. (2009). Physiological and morphological response of some grape cultivars to drought stress. Ph.D. Thesis Horticulture. University of Tehran. 181 p.
Rahman, A., Hossain, M. D. SH., Al-mahmud, J., Nahar, K., Hasanuzzaman, M. & Fujita, M. (2016). Manganese-induced salt stress tolerance in rice seedlings: regulation of ion homeostasis, antioxidant defense and glyoxalase systems. Physiology and Molecular Biology Plants, 22(3), 291-306.
Reyhanitabar, A. (2010). Kinetics of manganese release from some calcareous of Iran soils.  Water and Soil Science. 20(2), 131-142.
Sayed Tabatabaei, B. E. & Omidi, M. (2009). Plant Cell and Tissue Culture. Univercity of Tehran Press. (in Farsi)
Schurr, U., Heckenberger, U., Herdel, K., Walter, A. & Feil, R. (2000). Leaf development in Ricinus communis during drought stress: dynamics of growth processes, cellular structure, and sink–source transition. Journal of Experimental Botany, 51, 1515-1529.
Sebastian, A. & Prasad, M. N. V. (2015). Iron-and manganese-assisted cadmium tolerance in Oryza sativa L.: lowering of rhizotoxicity next to functional photosynthesis. Planta, 241, 1519-1528.
Shao, H. B., Chu, L. Y., Jaleel, C. A. & Zhao, C. X. (2008). Water-deficit stress-induced anatomical changes in higher plants. Plant Biology and Pathology, 331, 215-225.
Shitov, A. V., Pobeguts, O. V., Smolova, T. N., Allakhverdiev, S. I. & Klimov, V. V. (2009). Manganese-dependent carbohydrase activity of photosystem II proteins. Biochemistry Biokhimiia, 74(5), 509-517.
Soukhtesaraee, R., Ebadi, A., Salami, S. A & Lesani H. (2017). Evaluation of oxidative parameters in three grapevine cultivars under drought stress. Iranian Journal of Horticultural Sciences, 48(1), 85-98. (In Farsi)
Sukalovic, V. H. T., Vuletic, M., Veljovic-Jovanovic, S. & Vucinic, Z. (2010). The effects of manganese and copper in vitro and in vivo on peroxidase catalytic cycles. Journal of Plant Physiology, 167(18), 1550-1557.
Wang, Y. T., Wang, K. & Shao, X. Q. (2010). Manganese delays the senescence induced by drought in perennial ryegrass (Lolium perenne L.). African Journal of Agricultural Research, 5(22), 3035-3040.
Yemm, E. W. & Willis, A. J. (1954). The estimation of carbohydrates in plant extracts by anthrone. Biochemical Journal, 57, 508-514.
Yamasaki, S. & Dillenburg, L.C. (1999). Measurements of leaf relative water content in Araucaria angustifolia. Revista Brasileira de Fisiologia Vegetal, 11(2), 69-75.