تأثیر پرولین بر برخی ویژگی‌های فیزیولوژیکی و بیوشیمیایی دو رقم گل حنا (‏Impatiens ‎walleriana‏) تحت تنش شوری

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد، دانشکده کشاورزی، دانشگاه لرستان

2 استادیار و دانشیار، دانشکده کشاورزی، دانشگاه لرستان

3 دانشیار، دانشکده کشاورزی، دانشگاه لرستان

چکیده

به منظور مطالعه بررسی تأثیر محلول‎پاشی پرولین بر برخی ویژگی‎های فیزیولوژیکی و بیوشیمیایی  گل حنا، آزمایشی به ‎صورت فاکتوریل در قالب طرح کاملاً تصادفی با سه تکرار اجرا شد. عامل اول دو رقم گل حنا (Accent premium salmon و Tempo orange)، عامل دوم محلول‌پاشی پرولین (شاهد (صفر)، 5 و 10 میلی‎مولار به‌صورت هفتگی) وعامل سوم تنش شوری در چهار سطح (شاهد (صفر)، 20، 40 و 60 میلی‎مولار کلرید سدیم به صورت آبیاری سه روز یک‌بار (90 درصد ظرفیت زراعی)) بود. نتایج نشان داد افزایش تنش شوری موجب کاهش معنی‌دار وزن تر و خشک برگ، ساقه، ریشه و کل بوته، تعداد گل، قطر گل، زمان باز شدن گل، محتوای کلروفیل a، کلروفیل b، کلروفیل کل، کارتنوئید و فعالیت آنزیم کاتالاز و از سوی دیگر باعث افزایش قابل توجهی در میزان محتوای پرولین درونی، فعالیت آنزیم‌های پراکسیداز و آسکوربات پراکسیداز در هر دو رقم گل حنا شد. در حالی که اعمال تیمار پرولین اثرات تنش را تعدیل کرد و هر دو غلظت پرولین موجب افزایش قابل توجهی در همه‏ی ویژگی‌های مورد مطالعه نسبت به شاهد شد. بیشترین فعالیت آنزیم کاتالاز و مقدار پرولین در رقم سالمون و بیشترین فعالیت آنزیم آسکوربات پراکسیداز در رقم تمپو مشاهده شد. به‌طور کلی نتایج حاصل از این مطالعه نشان داد تنش شوری در همه‏ی سطوح دارای اثرات منفی بر رشد و عملکرد در هر دو رقم گل حنا بود، در صورتی که کاربرد پرولین به‌ویژه در غلظت 10 میلی‏مولار باعث کاهش اثرات منفی تنش شوری و افزایش مقاومت گیاه به تنش شوری شد.

کلیدواژه‌ها

عنوان مقاله [English]

Effect of proline on some physiological and biochemical characteristics of two ‎cultivars of Impatiens walleriana under salt stress

نویسندگان [English]

  • Fatemeh Roozbahani 1
  • Sadegh Mousavi-Fard 2
  • Abdolhossein Rezaeinejad 3
1 M.Sc. Student, Faculty of Agriculture, Lorestan University, Khoramabad, Iran
2 Assistant Professor, Faculty of Agriculture, Lorestan University, Khoramabad, Iran
3 Associate Professor, Faculty of Agriculture, Lorestan University, Khoramabad, Iran
چکیده [English]

The aim of this study was to investigate the effect of foliar application of proline on some physiological and biochemicalcharacteristics of two cultivars of impatiens under salinity stress. The experiment was a factorial based on a completely randomized design with three replications. The first factor was two cultivars of impatiens (Accent Premium Salmon and Tempo Orange), the second factor was foliar application of proline (0 as control, 5 and 10 mM were applied weekly) and the third factor was salinity stress at four levels (0 as control, 20. 40 and 60 mM sodium chloride were applied as irrigation (90% field capacity) every three day). The results showed that increasing salinity stress significantly reduced fresh and dry weight of leaf, stem, root and total plant, time of flower opening, flower diameter, number of flower, chlorophyll a, chlorophyll b, total chlorophyll, carotenoid content and catalase activity, and while significantly increased proline content, activity of peroxidase and ascorbate peroxidase enzymes in both cultivars of impatiens. Exogenous application of 5 and 10 mM proline have mitigated salinity stress effects and caused a significant increase in all of the studied characteristics. The highest activity of catalase and proline content was observed in Salmon cultivar, whereas the highest activity of ascorbate enzyme was observed in Tampo cultivar. In general, the results of this study indicated that all levels of salinity stress had negative effects on growth and yield of both impatiens cultivars, but proline application especially at a concentration of 10 mM reduced the effects of salinity stress and increased plant toleranceto stress.

کلیدواژه‌ها [English]

  • Antioxidant enzymes
  • Salmon
  • sodium chloride
  • Tempo

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علوم باغبانی ایران
دوره 51، شماره 3
آذر 1399
صفحه 537-549

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  • تاریخ دریافت: 26 اردیبهشت 1398
  • تاریخ بازنگری: 24 مهر 1398
  • تاریخ پذیرش: 18 آبان 1398

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