تأثیر محرک‌های زیستی هیومی فورته و‎ ‎اسید آمینه ال-آرژنین بر صفات رشدی، فیزیولوژیکی و ‏بیوشیمیایی جعفری آفریقایی (‏Tagetes erecta L.‎‏) تحت تنش خشکی

نوع مقاله: مقاله کامل

نویسندگان

1 دانشجوی کارشناسی ارشد، دانشکده علوم کشاورزی و صنایع غذایی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، ‏تهران، ایران

2 استادیار، دانشکده علوم کشاورزی و صنایع غذایی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران

3 استادیار گروه کشاورزى، دانشگاه پیام نور، تهران

4 استاد، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج، ایران

چکیده

به منظور بررسی اثر محرک زیستی هیومی فورته و اسید آمینه آرژنین بر خصوصیات رشدی، فیزیولوژیکی و بیوشیمیایی گل جعفری آفریقایی (Tagetes erecta L.) در شرایط تنش خشکی، آزمایشی به‌صورت فاکتوریل در قالب طرح پایه کاملا تصادفی در 3 تکرار انجام شد. عامل اول هیومی فورته در سه سطح (0، 5/2 و 5 میلی­گرم در لیتر)، عامل دوم اسیدآمینه ال-آرژنین در سه سطح (0، 5/1 و 3 میلی­مولار ) و عامل سوم تنش خشکی در سه سطح (100، 70 و 40 درصد ظرفیت زراعی) بودند. صفات تعداد گل، قطر گل­، کلروفیل کل، کاروتنوئید، فعالیت آنزیم سوپر اکسید دیسموتاز و مقدار عناصر پرمصرف (نیتروژن، پتاسیم و فسفر) در برگ اندازه گیری شدند. نتایج تجزیه واریانس نشان داد اثر هیومی فورته و خشکی روی تعداد و قطر گل معنی­دار شد (05/0P≤). تنش خشکی روی تمامی صفات مورد بررسی تأثیر معنی­داری داشت به‌طوریکه تنش 40 درصد ظرفیت زراعی سبب کاهش تعداد و قطر گل، کلروفیل کل و عناصر پرمصرف شد، اما سبب افزایش فعالیت آنزیم سوپر اکسید دیسموتاز و کاروتنوئید گردید. نمونه­های تیمار شده با هیومی فورته 5 میلی­گرم در لیتر بیشترین تأثیر بر صفات آزمایشی داشتند. اثرات سه­گانه هیومی­فورته، ال-آرژرنینوخشکی روی کلروفیل کل، فعالیت آنزیم سوپراکسید دیسموتاز، فسفر و پتاسیم معنی­دار شد (05/0P≤). تیمار ترکیبی هیومی فورته 5 میلی­گرم در لیتر به علاوه ال-آرژنین 3 میلی­مولار مناسب‌ترین تیمار جهت کاهش آثار سوء ناشی از تنش به‌ویژه تنش 40 درصد ظرفیت زراعی روی گیاه جعفری آفریقایی می­باشد. 

کلیدواژه‌ها


عنوان مقاله [English]

The effect of humi-forthi and L-arginine amino acid on growth, physiological and ‎biochemical characteristics of Marigold (Tagetes erecta) under drought stress

نویسندگان [English]

  • Mahsa Rezasefat 1
  • Sepideh Kalatejari 2
  • Foad Fatehi 3
  • Ahmad Khalighi 4
1 M.Sc. Student, Faculty of Agricultural Sciences and Food Industry, Islamic Azad University, Science and ‎Research Branch, Tehran, Iran
2 Assistant Professor, Faculty of Agricultural Sciences and Food Industry, Islamic Azad University, Science and Research Branch, ‎Tehran, Iran‎
3 Assistant Professor, Faculty of Agriculture, Payame Noor university, Tehran, Iran
4 Professor, Department of Horticulture, College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
چکیده [English]

In order to investigate effect of humi-forthi biostimulator and L-arginine amino acid on growth, physiological and biochemical characteristics of marigold (Tagetes erecta) under drought stress, a factorial experiment based on a completely randomized design with three replications was carried out. The first factor was humi-forthi at three levels (0, 2.5 and 5 mg/l), the second factor was L-arginine at three levels (0, 1.5 and 3 mM), and the thirs factor was drought stress at three levels (100%, 70%, and 40% FC). Traits including the number of flowers, flower diameter, total chlorophyll content, SOD enzyme activities, andNPK concentrations were measured. The results indicated that drought stress, especially 40% FC, significantly influenced all morpho-physiological traits of marigold. There was observed a reduction flower diameter and number, chlorophyll content, and NPK under drought stress, whereas an increase was recorded for SOD activity and carotenoid. In most traits, 5 mg/l humi-forthi was more effective in respect to 2.5 mg/l and control. In addition, 3 mM L-arginine significantly had greater impact in respect to control and 1.5 mM. The interaction of humi-forthi, L-arginine and drought stress was significant on total chlorophyll, SOD activity, P and K (P≤0.05). The treatment of 5 mg/l humi-forthi + 3 mM L-arginine is the most effective treatment for alleviating the adverse effect of drought stress particularly 40 % FC.  

کلیدواژه‌ها [English]

  • Drought stress
  • humi-forthi
  • L-arginine
  • marigold‎
  1. Alcázar, R., Planas, J., Saxena, T., Zarza, X., Bortolotti, C., Cuevas, J. & Altabella, T. (2010). Putrescine accumulation confers drought tolerance in transgenic Arabidopsis plants over-expressing the homologous Arginine decarboxylase 2 gene. Plant Physiology and Biochemistry, 48(7), 547-552.
  2. Amiri, R., Nikbakht, A. & Etemadi, N. (2015). Alleviation of drought stress on rose geranium [Pelargonium graveolens (L.) Herit.] in terms of antioxidant activity and secondary metabolites by mycorrhizal inoculation. Scientia Horticulturae, 197, 373-380.
  3. Bhattacharyya, M. (2017). Use of marigold (Tagetes sp.) for the successful control of nematodes in agriculture. The Pharma Innovation, 6(11, Part A), 1.
  4. Bouvier, F., Isner, J. C., Dogbo, O. & Camara, B. (2005). Oxidative tailoring of carotenoids: a prospect towards novel functions in plants. Trends in Pant Science, 10(4), 187-194.
  5. Cak Mak, I., Wolfgang, H.P. & Bonnie, M.C. (2010). Biofortification of durum wheat with zinc and iron. Cereal Chemistry, 87(1), 10-20.
  6. Chaves, M. M., Pereira, J. S., Maroco, J., Rodrigues, M. L., Ricardo, C. P. P., Osório, M. L. & Pinheiro, C. (2002). How plants cope with water stress in the field? Photosynthesis and growth. Annals of Botany, 89(7), 907-916.
  7. Escalona, A., Salas-Sanjuán, M. C., Santos, C. D. & Guzmán, M. (2014). The effect of water salinity on growth and ionic concentration and relation in plant tissues in Zinnia elegans and Tagetes erecta for use in urban landscasping. ITEA, 110(4), 325-334.
  8. Faraloni, C. & Torzillo, G. (2017). Synthesis of antioxidant carotenoids in microalgae in response to physiological stress. Carotenoids. InTechOpen, 143-157.
  9. Fox, K. F., Balevičius, V., Chmeliov, J., Valkunas, L., Ruban, A. V. & Duffy, C. D. (2017). The carotenoid pathway: what is important for excitation quenching in plant antenna complexes?. Physical Chemistry Chemical Physics, 19(34), 22957-22968.
  10. Guerra-Guimarães, L., Pinheiro, C., Chaves, I., Barros, D. R. & Ricardo, C. P. (2016). Protein dynamics in the plant extracellular space. Proteomes, 4(3), 22.
  11. Hussain, M. I., Shah, S. H., Hussain, S. A. J. J. A. D. & Iqbal, K. H. A. L. I. D. (2002). Growth, yield and quality response of three wheat (Triticum aestivum L.) varieties to different levels of N, P and K. International Journal of Agriculture and Biology, 4(3), 362-364.
  12. Jiang, Y. & Huang, B. (2001). Drought and heat stress injury to two cool-season turfgrasses in relation to antioxidant metabolism and lipid peroxidation. Crop Science, 41(2), 436-442.
  13. Liao, P., Liu, D., Xu, T. R., Yang, Y. & Cui, X. (2017). Soil water stress attenuate the growth and development but enhance the saponin synthesis of Panax notogesing during flowering stage. Industrial Crops and Products, 108, 95-105.
  14. Liu, F., Xing, S., Ma, H., Du, Z. & Ma, B. (2013). Cytokinin-producing, plant growth-promoting rhizobacteria that confer resistance to drought stress in Platycladus orientalis container seedlings. Applied Microbiology and Biotechnology, 97(20), 9155-9164.
  15. Mirseyedi, S. K., Nasiri, Y., Morshedloo, M. R. & Khalili, M. (2020). Evaluation of organic, chemical, biological and amino acids application on ‎quantitative and qualitative characteristics of chamomile (Matricaria chamomilla ‎L.) at different harvesting. Iranian Journal of Horticaltural Sciences, 50(4), 755-767. (in Farsi)
  16. Naghdibadi, H. A., Labafi, M. R., Ghavami, N., Ghaderi, A., Abdosi, V. & mehrafrin, A. (2015). Phytochemical and morphological responses of Thymus vulgaris L. for biomimetic sprays based on amino acids and methanol, Iranian Journal of Medical and AromaticPlants. 14(54), 146-158. (in Farsi)
  17. Nathan L. J. (2011). Arginine Amino Acid, Nova Science Publishers, 267 p.
  18. Pan, Y., Wu, L. J. & Yu, Z. L. (2006). Effect of salt and drought stress on antioxidant enzymes activities and SOD isoenzymes of liquorice (Glycyrrhiza uralensis Fisch). Plant Growth Regulation, 49(2-3), 157-165.
  19. Park, Y. J., Park, S. Y., Valan Arasu, M., Al-Dhabi, N. A., Ahn, H. G., Kim, J. K. & Park, S. U. (2017). Accumulation of carotenoids and metabolic profiling in different cultivars of tagetes flowers. Molecules, 22(2), 313.
  20. Porcel, R. & Ruiz-Lozano, J. M. (2004). Arbuscular mycorrhizal influence on leaf water potential, solute accumulation, and oxidative stress in soybean plants subjected to drought stress. Journal of Experimental Botany, 55(403), 1743-1750.
  21. Ramroodi, M., Rezaieenia, N., Gloeie, M. & Frozandeh, M. (2017). The effect of biological fertilizers on physiological properties and nutrients uptake of Cichorium intibus under drought stress. Iranian Journal of Field Crops Research, 15(4), 25-32. (in Farsi)   
  22. Sauheitl, L., Glaser, B. & Weigelt, A. (2009). Advantages of compound‐specific stable isotope measurements over bulk measurements in studies on plant uptake of intact amino acids. Rapid Communications in Mass Spectrometry, 23(20), 3333-3342.
  23. Shafiei, N., Khaleghi, E. & Moallemi, N. (2019). Effect of salicylic acid on some morphological and physiological characteristics of olive cv. ‘Konservalia’ under water deficit condition. Iranian Journal of Horticaltural Sciences, 49(4), 881-890.  (in Farsi)
  24. Shekari, F., Mehrafarin, A., Naghdibadi, H.A. & Hajiaghaie, R. (2014). Foliar application of biotic stimulators on yield and yield components of Plantago psyllium L. Iranian Journal of Medical and Aromatic Plants, 30(5), 811-820. (in Farsi)  
  25. Winter, G., Todd, C.D., Trovato, M., Forlani, G. & Funck, D. (2015). Physiological implications of arginine metabolism in plants. Frontiers in Pant Science, 6, 223-231.
  26. Yamori, W. & Shikanai, T. (2016). Physiological functions of cyclic electron transport around photosystem I in sustaining photosynthesis and plant growth. Annual Review of Plant Biology, 67, 81-106.
  27. Zeier, J. (2013). New insights into the regulation of plant immunity by amino acid metabolic pathways. Plant, Cell & Environment, 36(12), 2085-2103.