ازدیاد درون‌شیشه‌ای و سازگاری آدنیوم به‌عنوان یک گیاه زینتی ارزشمند

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانش‌آموخته کارشناسی ارشد، دانشکده کشاورزی و منابع طبیعی، دانشگاه اراک

2 استادیار، دانشکده کشاورزی و منابع طبیعی، دانشگاه اراک

چکیده

شرایط کشت درون‌شیشه‌ای آدنیوم به­عنوان یک گیاه زینتی وارداتی با ضریب ازدیاد کم، می‌تواند زمینه خوبی را برای تکثیر انبوه در تولید این گیاه ارزشمند فراهم آورد. بدین منظور این پژوهش به صورت فاکتوریل در قالب طرح کاملاً تصادفی طی آزمایش‌های شاخساره­زایی، پرآوری و ریشه­زایی جهت بهینه‌کردن اندام­زایی در دو گونه آبسوم و عربیکوم انجام شد. بیشترین شاخساره‌زایی در محیط کشت MS دارای BA 5/0 میلی‌گرم در لیتر برای گونه آبسوم و برای گونه عربیکوم در محیط کشت MS دارای 5/0 میلی‌گرم در لیتر BA به‌همراه 5/0 میلی‌گرم در لیتر TDZ بود. اندام‌زایی در گونه عربیکوم بیشتر به­صورت برگ و ریشه مشاهده شد و انگیزش شاخساره در آن نسبت به گونه آبسوم در مدت زمان طولانی‌تر رخ داد. نتایج نشان داد می‌توان از غلظت 5/0 میلی­گرم در لیتر BA برای پرآوری شاخساره‌­های انگیزش یافته در هر دو گونه آدنیوم استفاده کرد. بهترین تیمار در مرحله ریشه‌زایی محیط MS کامل به‌همراه 3/0 درصد زغال فعال بدون تنظیم‌کننده رشد حاصل شد. سازگاری تدریجی گیاهچه‌ها ابتدا در پرلیت استریل دارای MS یک دوم غلظت و سپس در بستر کشت خاک باغچه، ورمی‌کمپوست و پرلیت انجام گرفت.

کلیدواژه‌ها


عنوان مقاله [English]

In vitro propagation and acclimatization of Adenium sp. as valuable ornamental ‎plant

نویسندگان [English]

  • Fatemeh Zareian 1
  • Mina Taghizadeh 2
  • Alireza Khaleghi 2
1 Former M. Sc. Student, Faculty of Agriculture and Natural Resources, Arak University, Arak, Iran
2 Assistant Professor, Faculty of Agriculture and Natural Resources, Arak University, Arak, Iran
چکیده [English]

Optimizing the tissue culture condition for Adenium sp as an imported ornamental plant with a low propagation rate can deliver the way for mass propagation to multiplication of this ornamental plant. So, this research was carried out in a factorial experiment based on a completely randomized design to optimize organogenesis of Adenium abessum and Adenium arabicum during shoot induction, proliferation and root induction experiments. The highest rate of shoot regeneration for Adenium obesum was achieved in MS medium containing 0.5 mgl-1 BA, and for Adenium arabicum was achieved in MS medium containing 0.5 mgl-1 BA in combination with 0.5 mgl-1 TDZ. Organogenesis was more observed as leafy and rooted in A. arabicum and shoot induction in that was longer compare with A. abessum. The result showed that 0.5 mgl-1 BA can be used to prolifered shoots induced in both specie of Adenium. The full strength of free plant growth regulator MS medium supplemented with 0.3% activated charcoal was the best treatment at root induction stage. The plantlet gradually became first adapted in sterile perlite with 1/2 MS concentration and then in the culture media contained soil, vermicompost and perlite.

کلیدواژه‌ها [English]

  • Desert rose
  • callus
  • cytokinine
  • micropropagation
  • shoot regeneration
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