بهینه‌سازی ازدیاد درون‌شیشه‌ای گل صد تومانی (‏Paeonia mascula Mill (L.)‎‏) ‏

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری مهندسی علوم باغبانی (گیاهان زینتی)، گروه علوم باغبانی، پردیس دانشگاهی، دانشگاه گیلان، رشت

2 استادیار، گروه علوم باغبانی، دانشکده علوم کشاورزی، دانشگاه گیلان، رشت

3 استاد، سازمان جنگل‌ها و مراتع کشور، گیاه‌شناس سیستماتیک گیاهی، باغ ملی گیاه‌شناسی پیکان‌شهر، تهران

4 استادیار، گروه فیتوشیمی، پژوهشکده گیاهان دارویی، دانشگاه شهید بهشتی، تهران

چکیده

این پژوهش در دو مرحله جهت بهینه­سازی تکثیر درون‌شیشه‌ای گل صدتومانی بومی ایران انجام شد. در مرحله اول اثر غلظت‌های مختلف بنزیل آمینوپیورین (BAP) (صفر، 5/0،  و 2 میلی‌گرم در لیتر)، نفتالن استیک اسید (NAA) (صفر، 1/0، 2/0 و 3/0 میلی‌گرم در لیتر) و جیبرلیک اسید (GA3) (صفر و 5/0 میلی­گرم در لیتر) روی جوانه­های انتهایی گل صد تومانی گونه mascula به‌منظور باززایی مورد مطالعه قرار گرفت و در مرحله دوم اثر غلظت‌های مختلف BAP (صفر، 5/0، 1 و 2 میلی‌گرم در لیتر) و NAA (صفر، 25/0، 5/0 و 1 میلی­گرم در لیتر) روی القای کالوس‌های جنینی از برگ‌های جوان بررسی شد. در تمام آزمایش‌ها محیط کشت پایه MS مورد استفاده قرار گرفت. در مرحله اول بیشترین تعداد شاخه­ (38/5) در یک میلی­گرم در لیتر BAP به همراه 1/0 میلی­گرم در لیتر NAA و 5/0 میلی‌گرم در لیتر GA3 به‌دست آمد. نتایج نشان داد که افزایش غلظت BAP باعث کاهش طول شاخساره شد. حداکثر فراوانی کالوس جنین­زای متراکم (93/17) و تعداد جنین سوماتیکی در هر ریزنمونه (11/7) در تیمار 2 میلی­گرم در لیتر BAP و 5/0 میلی­گرم در لیتر NAAبه‌دست آمد. حداکثر درصد ریشه‌زایی (51/70 درصد) و تعداد ریشه (02/5) با 25/0 میلی‌گرم در لیتر NAAبه‌دست آمد.

کلیدواژه‌ها

عنوان مقاله [English]

Optimization in vitro micropropagation of peony (Paeonia mascula) ‎

نویسندگان [English]

  • Edris Mahdavi Fikijvar 1
  • Hedayat Zakizadeh 2
  • Valiollah Mozaffarian 3
  • Hassan Rezadoost 4
1 Ph.D. Candidate, Department of Horticultural Sciences, University Campus, University of Guilan, Rasht, Iran
2 Assistant Professor, Department of Horticultural Sciences, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran
3 Professor, Research Institute of Forests and Rangelands, Tehran, Iran
4 Assistant Professor, Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, ‎Evin, Tehran, Iran
چکیده [English]

This research was conducted in two stages to optimize in vitro propagation of Persian wild paeony. Stage 1: effect of BAP (0, 0.5, 1 and 2 mg.l-1), NAA (0, 0.1, 0.2 and 0.3 mg.l-1) and GA3 (0 and 0.5 mg.l-1) on apical bud ofPaeonia mascula and stage 2: effect of BAP (0, 0.5, 1 and 2 mg.l-1) and NAA (0, 0.25, 0.5 and 1 mg.l-1) were studied on embryonic callus induction on young leaves of Paonia mascula. At stage 1: the highest shoots number (5.38) was obtained on media containing 1 mg.l-1 BAP + 0.1 mg.l-1 NAA+ 0.5 mg.l-1 GA3. The results showed that increasing BAP level caused decreasing of shoot length. At stage 2: maximum of compact embryonic callus (17.93%) and somatic embryo number per explant (7.11) were obtained on 2 mg.l-1 BAP and 0.5mg.l-1 NAA. For callus proliferation, calli were maintained on MS medium containing 2 mg.l-1 BAP, 0.1 mg.l-1 NAA and 0.5 mg.l-1 GA3. Maximum rooting percentage (70.51%) and root number (0.0.02) were obtained with 0.25 mg.l-1 NAA.

کلیدواژه‌ها [English]

  • In vitro culture
  • organogenesis
  • somatic embryogenesis

مراجع

  1. Albers, M.R.J. & Kunneman, B.P.A.M. (1992). Micropropagation of Paeonia. Acta Horticulture, 314, 85-92.
  2. An, B.Y. (2005). Studies on the establishment of in vitro regeneration system of Paeonia suffruticosa Andr. M.Sc. thesis. Northeast Forestry University, China.
  3. Bhaskaran, S. & Smith, R.H. (1990). Regeneration in cereal tissue culture: A review. Crop Science, 30, 1328-1336.
  4. Bhojwani, S.S. & Razdan, M.K. (1996). Plant Tissue Culture: Theory and Practice, a revised edition. Elsevier Science.
  5. Bouza, L., Jacques, M. & Miginiac, M. (1994a). In vitro propagation of Paeonia suffruticosa Andr. cv. ‘Mme de Vatry’: development effects of exogenous hormones during the multiplication phase. Scientia Horticulturae, 57, 241-251.
  6. Bouza, L., Jacques, M. & Miginiac, E. (1994b). Requirements for in vitro rooting of Paeonia suffruticosa Andr. cv. ‘Mme de Vatry’. Scientia Horticulturae, 58, 223-233.
  7. Buchheim, J.A.T. & Meyer, M.M. (1992). Micropropagation of peony (Paeonia spp.). In: Bajaj YPS (ed) Biotechnology in Agriculture and Forestry, Vol. 20 (pp. 269–285). Springer- Verlag, Berlin, Heidelberg.
  8. Chen, X.L. (2005). The preliminary research on tissue culture of Paeonia suffruticosa. MSc Thesis. Henan Agricultural University, China. 
  9. Fan, X.F. & Fan, X.L. (2005). Studies on the clonal bud of three species of peony. Journal of Longdong University, (Natural Science Edition), 15, 38-41
  10. Féher, A., Pasternak, T. P. & Dudits, D. (2003) Transition of somatic plant cells to an embryogenic state. Review of plant biotechnology and applied genetics, Plant Cell, Tissue and Organ Culture, 74(3), 201-228. 
  11. Gabryszewska, E. (1998). The influence of cytokinins, thidiazuron, paclobutrazol and red light on shoot proliferation of herbaceous peony cv. Jadwiga in vitro. Journal of Fruit Ornamental Plant Research, 6, 157-169.
  12. Gabryszewska, E. (2010). The effect of glucose and growth regulators on the organogenesis of Paeonia lactiflora Pall. in vitro. Journal of Fruit and Ornamental Plant Research, 18 (2), 309-320
  13. Gaj, M.D. (2004) Factors influencing somatic embryogenesis induction and plant regeneration with particular reference to Arabidopsis thaliana Heynt. Plant Growth Regulators, 43, 27-47.
  14. Grando, M. F., Franklin, C.I. & Shatters, R.G. (2002). Optimizing embryogenic callus production and plant regeneration from 'Tifton 9' bahiagrass (Paspalum notatum Flügge) seed explants for genetic manipulation. Plant Cell Tissue Organ Culture, 71, 213-222.
  15. Guo, F.Y. (2001). Study on tissue culture of Paeonia lactiflora. M.Sc. thesis. Beijing Forestry University, China.
  16. Hosoki, T., Ando, M., Kubara, T., Hamada, M. & Itami, M. (1989). In vitro propagation of herbaceous peony (Paeonia lactiflora Pall.) by a longitudinal shoot-split method. Plant Cell Report, 8, 243-246.
  17. Jin-ding, Thomas, J.A. & Meyer M, M. Jr. (1987) American Peony Society Bulletin, 263, 24-30.
  18. Karami, O. (2008). Induction of Embryogenic Callus and Plant Regeneration in Carnation (Dianthus caryophyllus L.). Journal of Biological Sciences, 8 (4), 68-72.
  19. Kartha, K.K., Michayluk, M.R., Rao, K.L., Gamborg, O.L. & Constabel, F. (1974). Callus formation and plant regeneration from mesophyll protoplasts of rape plant (Brassica napus L. cv. 'Zephyr'). Plant Science Letters, 3, 265-271.
  20. Kim, H.M., Shin, J.H. & Sohn, J.K. (2006) Cryopreservation of somatic embryos of the herbaceous peony (Paeonia lactiflora Pall.) by air drying. Cryobiology, 53, 69-74
  21. Kim, Y.S. & Lee, B.K. (1996) Somatic embryogenesis and plant regeneration in cotyledon culture of Paeonia albiflora. Journal of Korean Society of Horticultural Sciences, 37, 827-830.
  22. Kong, X.S. & Zhang, M.X. (1998). Fast propagation of tree peony. Northwest Horticulture, 3 (4), 87-89.
  23. Krikorian, A.D., Kelly, K. & Smith, D.L. (1987). Hormones in tissue culture and micropropagation. In: P.J. Davies (Editor), Plant Hormones and their Role in Plant Growth and Development. (pp. 593-613) Martinus Nijhoff, Dordrecht.
  24. Lee, B.K., Ko, J.A. & Kim, Y.S. (1992) Studies on the thidiazuron treatment of anther culture in Paeonia albiflora. Journal of Korean Society of Horticultural Sciences, 33, 384-395.
  25. Li, Y.M. (2004). Studies on the tissue culture of three cultivars of Paeonia suffruticosa Andr. MSc Thesis. Beijing University, China.
  26. Li, Z.J., Wang, G.D., Xin, S.L. & Chen, K.J. (2006). The new technology of propagation in the organization cultivation of Paeonia suffruticosa. Journal of Laiyang Agricultural College (Natural Science), 23, 122-125.
  27. Lin, J.J., Thomas, J.A. & Meyer, M.M. (1987). Tissue culture and embryoid formation in Paeonia lactiflora Pall. American Peony Society Bulletin, 263, 24-30.
  28. Liu, C.M, Xu, Z.H., Chua, N.H. (1993). Auxin polar transport is essential for the establishment of bilateral symmetry during early plant embryogenesis. Plant Cell, 5, 621-630.
  29. Maroofi, H. (2005). Record of Paeonia mascula (Paeoniaceae) from Iran. The Iran Journal of Botany, 11 (1), 97-99. Tehran.
  30. Meyer, M.M. (2012) Multiple shoot induction and rooting of Paeonia lactiflora ‘Da Fu Gui’.  African Journal of Biotechnology, 11 (41), 977-978.
  31. Mol, H. & Von Arnold, S. (1991). Origin and development of embryogenic cultures from seedling of Norway spruce (Picea abies). Journal of Plant Physiology, 138, 223-230.
  32. Murashige, T. & Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiology Plant, 15, 473-496.
  33. Nasri, F., Mortazavi, S.N., Ghaderi, N. & Javadi, T. (2013). Propagation in vitro of Alstroemeria ligtu Hybrid through direct organogenesis from leaf base. Journal of Horticultural Research, 21 (2), 23-30.
  34. Orlikowska, T., Marasek, A. & Kucharska, D. (1998). Regeneration of Paeonia mloksewitschii Lom. and P. tenuifolia L. in vitro from different explants. Acta Societatis Botanicorum Poloniae, 67, 223-227.
  35. Page M. 2005. The Gardener’s Peony: Herbaceous and Tree Peonies. Portland (OR): Timber Press.
  36. Qin, K.J. (2004). Herbaceous peony. Beijing, China, Forestry Press, P. 76.
  37. Radtke, G.M. (1983) Tissue culture of herbaceous peonies. American Peony Society Bulletin, 246, 19-23.
  38. Rajabpoor, S., Azghandi, A.V. & Saboora, A. (2007) Effects of different concentrations of 2,4-D and BAP on somatic embryogenesis induction in saffron (Crocus sativus L.). Pakistsan Journal of Biological Science, 10, 3927-3930.
  39. Rahman M. S., Islam M. N., Tahar A. & Karim M. A. (2004) Influence GA, and MH and their time of spray on morphology, yield contributing characters and yield of Soybean. Asian Journal of plant Science, 3, 602-609.
  40. Rather, Z.A., Nazki, I.T. Qadri, Z.A. Mir M.A. Bhat K.M. & Hussain G. (2014). In vitropropagation of herbaceous peony (Paeonia lactiflora Pall.) cv. Sara Bernhardt using shoot tips. Indian Journal of Horticulture, 71 (3), 385-389.
  41. Roberts, M. & Sunderland, N. (1977) Pollen culture of Paeonia. John Innes Inst Annual Report, 68, 60-61.
  42. Sahrawat, A.K. & Chand, S. (2001) Continuous somatic embryogenesis and plant regeneration from hypocotyls segments of Psoralea corylifolia Linn., an endangered and medicinally important Fabaceae plant. Current Science, 81, 1328-1331.
  43. Shi, X.Q. & Zhang, Z.X. (2005). Callus culture of medicine organ of peony (Fenghuang Mountains). Acta Agricultural Nucleatae Sin, 19, 186-190Sonali, J., Sivanesan, I., Lim, L.I. & Jeong, B.R. (2013). In vitro zygotic embryo germination and somatic embryogenesis through cotyledonary explants of Paeonia lactiflora Pall. Flower Research Journal, 21 (1), 17-22.
  44. Tariq Hossain, H. M. M., Kim, Y. H. & Lee, Y. S. (2010). The apical bud as a novel explant for high-frequency in vitro plantlet regeneration of Perilla frutescens L. Britton. Plant Biotechnology Report, 4, 229-235.
  45. Tian, D. (2008). Container production and post-harvest handling of lotus (Nelumbo) and micropropagation of herbaceous peony (Paeonia). Ph.D. dissertation. Auburn University, Alabama, USA.
  46. Tian, D., Tilt, K.M., Dane, F., Woods, F.M. & Sibley, J.L. (2010). Comparison of shoot induction ability of different explants in herbaceous peony (Paeonia lactiflora Pall.). Scientia Horticulturae, 123, 385-389.
  47. Verma, S.K., Yucesan, B.B., Gurel, S. & Gurel, E. (2011). Indirect somatic embryogenesis and shoot organogenesis from cotyledonary leaf segments of Digitalis lamarckii Ivan., an endemic medicinal species. Turkish Journal of Biology, 35, 743-750.
  48. Wang, H.Y., He, S.L., Tanaka, M., Pham, T.V. & Teixeira da Silva JA (2010). Effects of 2, 4-D on callus formation in tree peony (Paeonia suffruticosa) under different light conditions and light quality. Floriculture Ornamental Biotechnology, 4 (1), 99-102.
  49. Wang, H.Y., He, S.L., Tanaka, M., Pham, T.V. & Teixeira da Silva, J.A. (2012). Effect of IBA concentration, carbon source, substrate, and light source on root induction ability of tree peony (Paeonia suffruticosa Andr.) plantlets in vitro. European Journal of Horticultural Sciences, 77, 122-128.
  50. Wang, Q. (2007). Peony tissue culture and rapid propagation. MSc Thesis. Henan Normal University, China.
  51. Werner, T & Schmülling T. (2009). Cytokinin action in plant development. Current Opinion in Plant Biology, 12, 527 538.
  52. Wu, H.J., Yu, X.N., Teixeira da Silva J.A. & Lu, J.P. (2011). Direct shoot induction of Paeonia lactiflora ‘Zhong Sheng Fen’ and rejuvenation of hyperhydric shoots. New Zealand Journal of Crop and Horticultural Science, 39, 271-278.
  53. Xie, J.X. (1987). Tissue culture of Paeonia suffruticosa. Plant Physiology Communication, 2, 53.
  54. Yu, X.N., Wu, H.J., Cheng, F.Y., Teixeira da Silva, J.A. & Shen, M.M. (2011). Studies on multiple shoot induction and proliferation of Paeonia lactiflora Pall. ‘Zhong Sheng Fen’. Propagation of Ornamental Plants, 11 (3), 144-148.
  55. Zaerr, J. B. & Mapes, M. O. (1982). Action of Growth regulators. In: J.M. Bonga & D. J. Durzan (Eds) Tissue Culture in Forestry (pp. 231-255) Dr. W. Junk Publishers, The Hague.
  56. Zhang, G.H., Wang, H.M. & Wang, L.X. (2001). Study on tissue culture techniques for peony. Shandong Agricultural Sciences, 5, 16-18
  57. Zhang, Q.R. (2006). The preliminary research on tissue culture of Paeonia lactiflora Pall. MSc Thesis. Henan Agricultural University, China.
علوم باغبانی ایران
دوره 51، شماره 1
خرداد 1399
صفحه 153-164

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  • تاریخ دریافت: 10 اسفند 1396
  • تاریخ بازنگری: 16 دی 1397
  • تاریخ پذیرش: 20 بهمن 1397

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