بهینه‌سازی ازدیاد درون‌شیشه‌ای گل صد تومانی (‏Paeonia mascula Mill (L.)‎‏) ‏

نوع مقاله: مقاله کامل

نویسندگان

1 دانشجوی دکتری مهندسی علوم باغبانی (گیاهان زینتی)، گروه علوم باغبانی، پردیس دانشگاهی، دانشگاه گیلان، رشت

2 استادیار، گروه علوم باغبانی، دانشکده علوم کشاورزی، دانشگاه گیلان، رشت

3 استاد، سازمان جنگل‌ها و مراتع کشور، گیاه‌شناس سیستماتیک گیاهی، باغ ملی گیاه‌شناسی پیکان‌شهر، تهران

4 استادیار، گروه فیتوشیمی، پژوهشکده گیاهان دارویی، دانشگاه شهید بهشتی، تهران

چکیده

این پژوهش در دو مرحله جهت بهینه­سازی تکثیر درون‌شیشه‌ای گل صدتومانی بومی ایران انجام شد. در مرحله اول اثر غلظت‌های مختلف بنزیل آمینوپیورین (BAP) (صفر، 5/0،  و 2 میلی‌گرم در لیتر)، نفتالن استیک اسید (NAA) (صفر، 1/0، 2/0 و 3/0 میلی‌گرم در لیتر) و جیبرلیک اسید (GA3) (صفر و 5/0 میلی­گرم در لیتر) روی جوانه­های انتهایی گل صد تومانی گونه mascula به‌منظور باززایی مورد مطالعه قرار گرفت و در مرحله دوم اثر غلظت‌های مختلف BAP (صفر، 5/0، 1 و 2 میلی‌گرم در لیتر) و NAA (صفر، 25/0، 5/0 و 1 میلی­گرم در لیتر) روی القای کالوس‌های جنینی از برگ‌های جوان بررسی شد. در تمام آزمایش‌ها محیط کشت پایه MS مورد استفاده قرار گرفت. در مرحله اول بیشترین تعداد شاخه­ (38/5) در یک میلی­گرم در لیتر BAP به همراه 1/0 میلی­گرم در لیتر NAA و 5/0 میلی‌گرم در لیتر GA3 به‌دست آمد. نتایج نشان داد که افزایش غلظت BAP باعث کاهش طول شاخساره شد. حداکثر فراوانی کالوس جنین­زای متراکم (93/17) و تعداد جنین سوماتیکی در هر ریزنمونه (11/7) در تیمار 2 میلی­گرم در لیتر BAP و 5/0 میلی­گرم در لیتر NAAبه‌دست آمد. حداکثر درصد ریشه‌زایی (51/70 درصد) و تعداد ریشه (02/5) با 25/0 میلی‌گرم در لیتر NAAبه‌دست آمد.

کلیدواژه‌ها


عنوان مقاله [English]

Optimization in vitro micropropagation of peony (Paeonia mascula) ‎

نویسندگان [English]

  • Edris Mahdavi Fikijvar 1
  • Hedayat Zakizadeh 2
  • Valiollah Mozaffarian 3
  • Hassan Rezadoost 4
1 Ph.D. Candidate, Department of Horticultural Sciences, University Campus, University of Guilan, Rasht, Iran
2 Assistant Professor, Department of Horticultural Sciences, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran
3 Professor, Research Institute of Forests and Rangelands, Tehran, Iran
4 Assistant Professor, Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, ‎Evin, Tehran, Iran
چکیده [English]

This research was conducted in two stages to optimize in vitro propagation of Persian wild paeony. Stage 1: effect of BAP (0, 0.5, 1 and 2 mg.l-1), NAA (0, 0.1, 0.2 and 0.3 mg.l-1) and GA3 (0 and 0.5 mg.l-1) on apical bud ofPaeonia mascula and stage 2: effect of BAP (0, 0.5, 1 and 2 mg.l-1) and NAA (0, 0.25, 0.5 and 1 mg.l-1) were studied on embryonic callus induction on young leaves of Paonia mascula. At stage 1: the highest shoots number (5.38) was obtained on media containing 1 mg.l-1 BAP + 0.1 mg.l-1 NAA+ 0.5 mg.l-1 GA3. The results showed that increasing BAP level caused decreasing of shoot length. At stage 2: maximum of compact embryonic callus (17.93%) and somatic embryo number per explant (7.11) were obtained on 2 mg.l-1 BAP and 0.5mg.l-1 NAA. For callus proliferation, calli were maintained on MS medium containing 2 mg.l-1 BAP, 0.1 mg.l-1 NAA and 0.5 mg.l-1 GA3. Maximum rooting percentage (70.51%) and root number (0.0.02) were obtained with 0.25 mg.l-1 NAA.

کلیدواژه‌ها [English]

  • In vitro culture
  • organogenesis
  • somatic embryogenesis
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