بررسی اثر محیط کشت و تنظیم‌کننده‌های‎ ‎رشد گیاهی بر‎ ‎ریزازدیادی دو پایه جنس پرونوس ‏(‏‎ CAB 6P‎و ‏JASPI‏)‏

نوع مقاله: مقاله کامل

نویسندگان

1 دانشیار، پژوهشکده میوه‌های معتدله و سردسیری، مؤسسه تحقیقات علوم باغبانی، سازمان تحقیقات، آموزش و ترویج کشاورزی، ‏کرج، ایران ‏

2 دانشجوی سابق کارشناسی ارشد، دانشگاه آزاد اسلامی واحد کرج، کرج، ایران

3 دانشیار، پژوهشکده کشاورزی، سازمان پژوهش‌های علمی و صنعتی ایران، تهران، ایران

4 کارشناس ارشد مؤسسه تحقیقات باغبانی، پژوهشکده میوه‌های معتدله و سردسیری، مؤسسه تحقیقات علوم باغبانی، سازمان ‏تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران

چکیده

دو پایه رویشی CAB 6P و JASPI متعلق به جنس Prunus، از لحاظ پاکوتاه بودن بسیار قابل‌توجه می‌باشند. با توجه به اهمیت بالای استفاده از پایه‌های پاکوتاه در تولید تجاری درختان میوه و تکثیر انبوه آنها از طریق کشت بافت، این تحقیق با هدف مطالعه پرآوری، ریشه­زایی و سازگاری این دو پایه و به‌صورت فاکتوریل در قالب طرح کاملاً تصادفی، شامل محیط کشت­های MS و MS½ در ترکیب با غلظت­های مختلف هورمون BA (صفر، 5/0، 1 و 5/1 میلی­گرم در لیتر) و IBA (صفر، 1/0 و 2/0 میلی­گرم در لیتر) بر پرآوری و اثر محیط کشت­های WPM و MS در ترکیب با غلظت­های مختلف هورمون IBA و NAA (صفر، 5/0، 1 و 5/1 میلی­گرم در لیتر) بر ریشه­زایی دو پایه CAB 6P و JASPI انجام گرفت. براساس نتایج، محیط کشت MS حاوی یک میلی­گرم در لیتر BA + 2/0 میلی‌گرم در لیتر IBA برای مرحله پرآوری هر دو پایه و محیط کشت WPM حاوی 5/1 میلی‌گرم در لیتر IBA + 5/0 میلی­گرم در لیتر NAA و محیط کشت WPM حاوی 5/0 میلی‌گرم در لیتر IBA + 5/0 میلی­گرم در لیتر NAA به‏‌‏ترتیب برای ریشه­زایی دو پایه CAB 6P و JASPI توصیه می گردند.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of culture media and plant growth regulators on micropropagation of two ‎Prunus rootstocks (CAB 6p and JASPI) ‎

نویسندگان [English]

  • Naser Bouzari 1
  • Ramin Shafiei 2
  • Batool Hossinpour 3
  • Seyedeh Samaneh Hosseini 4
1 Associate Professor, Temperate and Cold Fruits Research Institute (TCFRI), Horticulture Science Research Institute, Karaj, Iran
2 Former M.Sc. Student, Islamic Azad University, Karaj Branch, Karaj, Iran‎
3 Associate Professor, Institute of Agriculture, Department of Plant Production, Iranian Research Organization for Science and ‎Technology (IROST), Tehran, Iran‎
4 M.Sc., Temperate and Cold Fruits Research Institute (TCFRI), Horticulture Science Research Institute, Karaj, Iran‎
چکیده [English]

The CAB 6P and JASPI rootstocks belongs to Prunus genus, are very noticeable in terms of dwarfing. Due to the importance of dwarf rootstocks in fruit trees commercial production and also their mass propagation by tissue culture, this research was conducted to study the proliferation, rooting and acclimatization of these rootstocks. A factorial experiment was carried out based on a completely randomized design, including the effect of MS and ½ MS media in combination with different concentrations of BA (0, 0.5, 1 and 1.5 mg/l) and IBA (0, 0.1 and 0.2 mg/l) on proliferation and also the effect of WPM and MS media in combination with different concentrations of IBA and NAA (0, 0.5, 1 and 1.5 mg/l) on rooting of two rootstock (JASPI and CAB 6P). Based on the results, MS medium with 1 mg/l BA + 0.2 mg/l IBA for proliferation of both rootstocks and WPM medium containing 1.5 mg/l IBA + 0.5 mg/l NAA and WPM medium containing 0.5 mg/l IBA+ 0.5 mg/l NAA are recommended for rooting stage of CAB 6P and JASPI rootstocks, respectively.

کلیدواژه‌ها [English]

  • BA
  • Cherry
  • dwarf rootstock
  • IBA
  • plant tissue culture
  • plum
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