بررسی اثر محیط کشت و تنظیم‌کننده‌های‎ ‎رشد گیاهی بر‎ ‎ریزازدیادی دو پایه جنس پرونوس ‏(‏‎ CAB 6P‎و ‏JASPI‏)‏

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشیار، پژوهشکده میوه‌های معتدله و سردسیری، مؤسسه تحقیقات علوم باغبانی، سازمان تحقیقات، آموزش و ترویج کشاورزی، ‏کرج، ایران ‏

2 دانشجوی سابق کارشناسی ارشد، دانشگاه آزاد اسلامی واحد کرج، کرج، ایران

3 دانشیار، پژوهشکده کشاورزی، سازمان پژوهش‌های علمی و صنعتی ایران، تهران، ایران

4 کارشناس ارشد مؤسسه تحقیقات باغبانی، پژوهشکده میوه‌های معتدله و سردسیری، مؤسسه تحقیقات علوم باغبانی، سازمان ‏تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران

چکیده

دو پایه رویشی CAB 6P و JASPI متعلق به جنس Prunus، از لحاظ پاکوتاه بودن بسیار قابل‌توجه می‌باشند. با توجه به اهمیت بالای استفاده از پایه‌های پاکوتاه در تولید تجاری درختان میوه و تکثیر انبوه آنها از طریق کشت بافت، این تحقیق با هدف مطالعه پرآوری، ریشه­زایی و سازگاری این دو پایه و به‌صورت فاکتوریل در قالب طرح کاملاً تصادفی، شامل محیط کشت­های MS و MS½ در ترکیب با غلظت­های مختلف هورمون BA (صفر، 5/0، 1 و 5/1 میلی­گرم در لیتر) و IBA (صفر، 1/0 و 2/0 میلی­گرم در لیتر) بر پرآوری و اثر محیط کشت­های WPM و MS در ترکیب با غلظت­های مختلف هورمون IBA و NAA (صفر، 5/0، 1 و 5/1 میلی­گرم در لیتر) بر ریشه­زایی دو پایه CAB 6P و JASPI انجام گرفت. براساس نتایج، محیط کشت MS حاوی یک میلی­گرم در لیتر BA + 2/0 میلی‌گرم در لیتر IBA برای مرحله پرآوری هر دو پایه و محیط کشت WPM حاوی 5/1 میلی‌گرم در لیتر IBA + 5/0 میلی­گرم در لیتر NAA و محیط کشت WPM حاوی 5/0 میلی‌گرم در لیتر IBA + 5/0 میلی­گرم در لیتر NAA به‏‌‏ترتیب برای ریشه­زایی دو پایه CAB 6P و JASPI توصیه می گردند.

کلیدواژه‌ها

عنوان مقاله [English]

Effect of culture media and plant growth regulators on micropropagation of two ‎Prunus rootstocks (CAB 6p and JASPI) ‎

نویسندگان [English]

  • Naser Bouzari 1
  • Ramin Shafiei 2
  • Batool Hossinpour 3
  • Seyedeh Samaneh Hosseini 4
1 Associate Professor, Temperate and Cold Fruits Research Institute (TCFRI), Horticulture Science Research Institute, Karaj, Iran
2 Former M.Sc. Student, Islamic Azad University, Karaj Branch, Karaj, Iran‎
3 Associate Professor, Institute of Agriculture, Department of Plant Production, Iranian Research Organization for Science and ‎Technology (IROST), Tehran, Iran‎
4 M.Sc., Temperate and Cold Fruits Research Institute (TCFRI), Horticulture Science Research Institute, Karaj, Iran‎
چکیده [English]

The CAB 6P and JASPI rootstocks belongs to Prunus genus, are very noticeable in terms of dwarfing. Due to the importance of dwarf rootstocks in fruit trees commercial production and also their mass propagation by tissue culture, this research was conducted to study the proliferation, rooting and acclimatization of these rootstocks. A factorial experiment was carried out based on a completely randomized design, including the effect of MS and ½ MS media in combination with different concentrations of BA (0, 0.5, 1 and 1.5 mg/l) and IBA (0, 0.1 and 0.2 mg/l) on proliferation and also the effect of WPM and MS media in combination with different concentrations of IBA and NAA (0, 0.5, 1 and 1.5 mg/l) on rooting of two rootstock (JASPI and CAB 6P). Based on the results, MS medium with 1 mg/l BA + 0.2 mg/l IBA for proliferation of both rootstocks and WPM medium containing 1.5 mg/l IBA + 0.5 mg/l NAA and WPM medium containing 0.5 mg/l IBA+ 0.5 mg/l NAA are recommended for rooting stage of CAB 6P and JASPI rootstocks, respectively.

کلیدواژه‌ها [English]

  • BA
  • cherry
  • dwarf rootstock
  • IBA
  • plant tissue culture
  • plum

مراجع

  1. Andreu, P. & Marín, J. A. (2005). In vitro culture establishment and multiplication of the Prunus rootstock ‘Adesoto 101’ (P. insititia L.) as affected by the type of propagation of the donor plant and by the culture medium composition. Scientia Horticulturae, 106(2), 258-267.
  2. Daneshvar Hosseini, A., Ganji Moghadam, E., Kavari Khorasani, S. & Bihamta, M. (2011). Effects of growth regulators on micro propagation of some Mahaleb dwarf genotypes (Prunus mahaleb L.). Archives of Applied Science Research, 3 (1), 118-125.
  3. Debergh, P., Aitken-Christie, J., Cohen, D., Grout, B., Von Arnold, S., Zimmerman, R. & Ziv, M. (1992). Reconsideration of the term ‘vitrification’as used in micropropagation. Plant Cell, Tissue and Organ Culture, 30(2), 135-140.
  4. Dejampour, J., Garigurian, V., Majidi, A. & Aliasgharzadeh, N. (2007). Sterilization, establishment and proliferation of some Prunus interspesific hybrids for In vitro culture. Iranian Journal of Horticultural Science and Technology, 8 (3), 165-174. (in Farsi)
  5. Dejampour, J., Garigurian, V., Majidi, A. & Onsorudi, F. (2010). Evaluation of in vitro rooting of some interspecific hybrids in Prunus genus. Iranian Journal of Horticultural Science and Technology, 11 (1), 1-10. (in Farsi)
  6. Dorić, D., Ognjanov, V., Ljubojević, M., Barać, G., Dulić, J., Pranjić, A., & Dugalić, K. (2014). Rapid propagation of sweet and sour cherry rootstocks. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 42 (2), 488-494.
  7. Druart, P. (2013). Micropropagation of Prunus species relevant to cherry fruit production. In: M. Lambardi, E.A. Ozudogru & S.M. Jain (Eds). Protocols for micropropagation of selected economically-important horticultural plants. (pp. 119-136). Humana Press, New York.
  8. Dziedzic, E. & Malodobry, M. (2006). Vegetative cherry rootstocks in tissue culture. Scientific Works of the Lithuanian Institute of Horticulture and Lithuanian University of Agriculture. Sodininkysté ir Daržininkysté, 25, 77-84.
  9. Edriss, M. H., Baghdadi, G. A., Abd, E. R. & Abdel-Aziz, H. F. (2014). Micropropagation of some peach rootstocks. Nature and Science, 12 (3), 106-114.
  10. Fallahpour, M., Miri, S. M. & Bouzari, N. (2015). Effect of culture medium and growth regulators on micpropogation of CAB-6P rootstock. In: 3rd National Symposium on Sustainable Agriculture and Natural Resources, 17June, Mehrarvand Institute of Technology, Tehran, Iran. (in Farsi)
  11. Gangi Moghadam, E., Bolandi, A.R. & Anahid, S. (2008). Micropropagation of four selected dwarf mahaleb (Prunus mahaleb L.) genotypes. Pajouhesh and Sazandegi, 79, 54-61 (in Farsi).
  12. George, E. F. & Sherrington, P. D. (1984). Plant propagation by tissue culture: handbook and directory of commercial laboratories, Exegetics Ltd. Press, Edington.
  13. George, E. F., Hall, M. A. & De Klerk, G. J. (2008). Plant growth regulators II: cytokinins, their analogues and antagonists. In: Plant propagation by tissue culture. (pp. 205-226.) Springer Netherlands.
  14. Hosseini, A. D., Moghadam, E. G. & Anahid, S. (2010). Effects of media cultures and plant growth regulators in micropropagation of Gisela 6 rootstock. Annals of Biological Research, 1(2), 135-141.
  15. Izadpanah, M. (2003). The proliferation of Ulmus glabra through in vitro culture. Pajouhesh and Sazandegi, 16 (1), 66-74. (in Farsi)
  16. Izadpanah, M. (2004). The effects of age and growth phase on micropropagation of Prunus avium through in vitro culture. Pajouhesh and Sazandegi, 64, 63-70. (in Farsi)
  17. Kamali, K., Mahidi, E. & Zarghami, R. (2001). Determination of the most suitable culture medium and growth conditions for micropropagation of Gf677 (Hybrid of Almond X Peach) rootstocks. Seed and Plant Improvement Journal, 17 (3), 234-243 (in Farsi)
  18. Kamali, K., Majidi, E., Zarghami, R. & Arvin, M. J. (2006) Differences in micropropagation of vegetative rootstock (GF677) and other almond seed genotypes. Acta Horticulturae, 726, 199-200.
  19. Karamad, Z., Ganji Moghadam, E. & Bolandi, A. (2013). Effects of culture media and growth regulators on micropropagation of Gisela 6 rootstock. Agricultural Crop Management 16 (2): 339-351. (in Farsi)
  20. Lloyd, G. & McCown, B.H. (1980) Commercially-feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot-tip culture. Combined Proceedings International Plant Propagator’s Society, 30, 421-427.
  21. Mahdavian, M., Bouzari, N. & Abdollahi, H. (2010). Effects of culture media and growth regulators on proliferation and rooting of a vegetative mahlab rootstock (SL-64). Seed and Plant Improvement Journal, 26-1 (1), 15-26. (in Farsi)
  22. Moubayidin, L., Di Mambro, R. & Sabatini, S. (2009). Cytokinin-auxin crosstalk. Trends in Plant Science, 14, 557-562.
  23. Murashige, T. & Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiology of Plants, 15, 473-97.
  24. Pruski, K. (2007). Tissue culture propagation of Mongolian cherry (Prunus fruticosa L.) and Nanking cherry (Prunus tomentosa L.). In: S. M. Jain, & H. Häggman, (Eds), Protocols for micropropagation of woody trees and fruits. (pp. 391-407.) Springer Netherlands.
  25. Pruski, K. W., Lewis, T., Astatkie, T. & Nowak, J. (2000). Micropropagation of Chokecherry and Pincherry cultivars. Plant Cell, Tissue and Organ Culture, 63(2), 93-100.
  26. Reighard, G. L., Ouellette, D. R. & Brock, K. H. (2006). Growth and survival of 20 peach rootstocks and selections in South Carolina. Acta Horticulturae, 713, 269-273.
  27. Rogalski, M., Moraes, L. K. A. D., Felisbino, C., Crestani, L., Guerra, M. P. & Silva, A. L. D. (2003). Acclimatization of micropropagated Prunus sp. rootstocks. Revista Brasileira de Fruticultura, 25(2), 279-281.
  28. Rustaei, M., Nazeri, S., Ghadimzadeh, M., & Hemmaty, S. (2009). Effect of phloroglucinol, medium type and some component on in vitro proliferation of dwarf rootstock of apple (Malus domestica). International Journal of Agriculture and Biology, 11, 193-196.
  29. Ružić, D., Sarić, M., Cerović, R. & Ćulafić, L. (2000). Relationship between the concentration of macroelements, their uptake and multiplication of cherry rootstock ‘Gisela 5’ in vitro. Plant Cell, Tissue and Organ Culture, 63 (1), 9-14.
  30. Ružić, D. V. & Vujović, T. I. (2008). The effects of cytokinin types and their concentration on in vitro multiplication of sweet cherry cv. Lapins (Prunus avium L). Horticultural Science, 35(1), 12-21.
  31. Sarropoulou, V., Dimassi-Theriou, K. & Therios, I. (2015). Medium strength in inorganics and PVP concentration effects on cherry rootstocks in vitro rooting. Horticultural Science, 42(4), 185-192.
  32. Sedlak, J., Papertine, F. & Erbenova, M. (2005). In vitro propagation dwarfing sweet cherry rootstock. Acta Horticulturae, 667, 217-222
  33. Su, Y.H., Liu, Y.B. & Zhang, X.S.H. (2011). Auxin-cytokinin interaction regulates meristem development. Molecular Plant, 4 (4), 616-625.
  34. Tatari Varnosfaderani, M., Mousavi, A. & Bouzari, N. (2012). Micropropagation of some clonal rootstocks of stone fruits. Seed and Plant Improvement Journal, 28-1 (1), 53-66. (in Farsi)
  35. Vertesy, J. 1981. In vitro propagation of Prunus persica and Prunus persico davidiana shoot tip in order to get virus free plants. Acta Horticulturae, 94, 261-266.
  36. Yang, Z.B., Liu, G., Liu, J., Zhang, B., Meng, W., Müller, B., Hayashi, K., Zhang, X., Zhao, Z.H., Smet, I.V. & Ding, Z.H. (2017). Synergistic action of auxin and cytokinin mediates aluminum-induced root growth inhibition in Arabidopsis. EMBO Reports, 18, 1213-1230.
  37. Zarrouk, O., Aparicio, J., Gogorcena, Y. & Moreno, M.A. (2006). Graft compatibility for new peach rootstocks in nursery. Acta Horticulturae, 713, 327-329.
علوم باغبانی ایران
دوره 50، شماره 4
اسفند 1398
صفحه 851-864

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سابقه مقاله

  • تاریخ دریافت: 25 شهریور 1397
  • تاریخ بازنگری: 01 دی 1397
  • تاریخ پذیرش: 22 دی 1397

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