اثر محلول پاشی لاکتات کلسیم بر خصوصیات فیزیولوژیکی، فعالیت آنتی اکسیدانی و عملکرد کاهو (Lactuca sativa L.) تحت شرایط کم آبیاری

نوع مقاله: مقاله کامل

نویسندگان

1 دانشجوی سابق کارشناسی ارشد، دانشکده کشاورزی، دانشگاه زنجان، زنجان، ایران

2 دانشیار، دانشکده کشاورزی، دانشگاه زنجان، زنجان، ایران

3 استادیار، دانشکده کشاورزی، دانشگاه زنجان، زنجان، ایران

چکیده

به­منظور ارزیابی اثر لاکتات­کلسیم بر برخی از ویژگی­های فیزیولوژیکی، آنتی­اکسیدانی و عملکرد کاهو تحت شرایط کم­آبیاری، آزمایشی به­صورت کرت­های خرد شده بر پایه طرح بلوک­های کامل تصادفی در سه تکرار در شرایط اقلیمی زنجان در سال 1396 اجرا شد. تیمار­های آزمایش شامل سه رژیم آبیاری مختلف (100، 85 و 70 درصد نیاز آبی گیاه) به­عنوان فاکتور اصلی و محلول­پاشی لاکتات­کلسیم در سه سطح (0، 75/0 و 5/1 گرم در لیتر) به­عنوان فاکتور فرعی بودند. نتایج نشان داد که تنش کم­آبیاری به­طور معنی‌داری فعالیت آنتی­اکسیدانی و میزان پرولین، فنل، فلاونوئید و فعالیت آنزیم­های پراکسیداز و کاتالاز را افزایش داد و شاخص پایداری غشا، محتوای نسبی آب برگ، باقیمانده آب برگ و عملکرد را کاهش داد. کاربرد برگی لاکتات­کلسیم به­طور معنی­داری وضعیت آب برگ، فعالیت آنتی­اکسیدانی و عملکرد گیاه را نسبت به تیمار شاهد افزایش داد. بیشترین مقدار پرولین و فعالیت آنزیم­های کاتالاز و پراکسیداز با کاربرد لاکتات­کلسیم 5/1 گرم در لیتر در شرایط کم­آبیاری 70 درصد حاصل شد. حداکثر فعالیت آنتی­اکسیدانی تحت 75/0 گرم در لیتر و شرایط کم­آبیاری 85 درصد به­دست آمد. با توجه به نتایج، کاربرد لاکتات­کلسیم 5/1 گرم در لیتر برای بهبود وضعیت آب برگ، فعالیت آنتی­اکسیدانی آنزیمی و غیرآنزیمی و عملکرد کاهو پیشنهاد می­گردد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Effect of foliar spray of calcium lactate on physiological characteristics, antioxidant activity and yield of lettuce (Lactuca sativa L.) under deficit irrigation

نویسندگان [English]

  • Arezoo Khani 1
  • Taher Barzegar 2
  • Jaefar Nikbakht 2
  • Zahra Ghahremani 3
1 Former M. Sc. Student, Department of Horticultural Sciences, Faculty of Agriculture, University of Zanjan, Zanjan, Iran
2 Associate Professor, Department of Horticultural Sciences, Faculty of Agriculture, University of Zanjan, Zanjan, Iran
3 Assistant Professor, Department of Horticultural Sciences, Faculty of Agriculture, University of Zanjan, Zanjan, Iran
چکیده [English]

In order to evaluate the effect of calcium lactate (Ca) on some physiological, antioxidant properties and yield of lettuce cv.New Red Fire under deficit irrigation, a split plot experiment based on randomized complete blocks design with three replications was conducted in Zanjan climatic conditions during 2017. Experimental treatments consisted of three different irrigation regimes (70, 85 and 100% ETc) as the main plot and calcium lactate at three levels (0, 0.75 and 1.5 g.l-1) as a sub plot. Results showed that deficit irrigation significantly increased the antioxidant activity, proline, phenol, flavonoids contents as well as peroxidase and catalase enzymes activity. In contrast, deficit irrigation reduced the membrane stability index, leaf relative water content, excised leaf water retention and yield. The foliar application of calcium lactate significantly increased plant water status, antioxidant activity and yield. The highest proline content and catalase and peroxidase enzymes activity was obtained with application of 1.5 g.l-1 Ca under deficit irrigation of 70% ETc. The maximum antioxidant activity was achieved under 0.75 g.l-1 Ca treatment and deficit irrigation of 85% ETc. According to results, application of 1.5 g.l-1 Ca can be proposed to improve plant water status, enzyme and non-enzyme antioxidant activity and yield of lettuce. 

کلیدواژه‌ها [English]

  • Antioxidant Enzymes
  • proline
  • relative water content
  • membrane stability
  1. Abedi, T. & Pakniyat, H. (2012). Antioxidant enzyme changes in response to drought stress in ten cultivar of oilseed rape (Brassica napus L.). Czech Journal of Genetics and Plant Breeding, 46(4), 27-34.
  2. Ahmadizadeh, M., Valizadeh, M., Zaefizadeh, M. & Shahbazi, H. (2011). Antioxidative protection and electrolyte leakage in durum wheat under drought stress condition. Journal of Applied Sciences Research, 7(3), 236-246.
  3. Alarcon, A. L., Madrid, R., Egea, C. & Guillen, I. (1999). Calcium deficiency provoked by the application of different forms and concentrations of Ca+2 to soilless cultivated muskmelons. Scientia Horticulturae, 81, 89-102.
  4. Alizadeh Ahmadabadi, A., Khorasani Nejad, S. & Hemati, Kh. (2016). Effect of deficit irrigation and ecosystemic stress on morphological and phytochemical characteristics of Echinacea purpurea. Agricultural Crop Management, 19(1), 1-14. (in Farsi)
  5. Anjali, S. & Kale, P. B. (2007). Response and recovery of Coriandrum sativum L. variety indoor exposed to soil moisture stress. Indian Journal of Plant Physiology, 12, 266-270.
  6. Anjum, N. A., Umar, S. & Chan, M. T.  (2010). Ascorbate-glutathione pathway and stress tolerance in plants. Springer Dordrecht Heidelberg, 265-291.
  7. Apel, K. & Hirt, H. (2004). Reactive oxygen spicies: metabolism, oxidative stress, and signal transduction. Plant Biology, 55, 373-399.
  8. Ashraf, M. (2010). Inducing drought tolerance in plants. Recent advances. Biotechnology Advances, 28, 169-183.
  9. Athukorala, Y., Kim, K. N. & Jeon, Y. J. (2006). Antiproliferative and antioxidant properties of an enzymatic hydroliysate from brown algae, Ecklonia cava. Food and Chemical Toxicology, 44, 1065-1074.
  10. Aydin, A., Kant, C. & Turan, M. (2012). Humic acid application alleviates salinity stress of bean (Phaseolus vulgaris L.) plants decreasing membrane leakage. African Journal of Agricultural Research, 7(7), 1073-1086.
  11. Azizpour, K., Shakiba, M. R., Khosh Kholgh, S. N., Alyari, H., Moghaddam, M., Esfandiari, E. & Pessarakli, M. (2010). Physiological response of spring durum wheat genotypes to salinity. Journal of Plant Nutrition, 33, 859-873.
  12. Barbagallo, R. N., Chisari, M. & Patané, C. (2012). Polyphenol oxidase total phenolics and ascorbic acid changes during storage of minimally processed ‘California Wonder’ and ‘Quadrato d'Asti’ sweet peppers. Food Science and Technology, 49, 192-196.
  13. Bates, L. S., Waldren, R. P. & Tevre I. V. (1973). Rapid determination of free proline for water- stress studies. Plant Soil, 39, 205- 207. 
  14. Bernstein, N., Shoresh, M., Xu, Y. & Huang, B. (2010). Involvement of the plant antioxidative response in the differential growth sensitivity to salinity of leaves vs roots during cell development. Free Radical Biology and Medicine, 49(7), 1161-1171.
  15. Blokhina, O., Virolainen, E. & Fagerstedt, K. (2003). Antioxidants, oxidative damage and oxygen deprivation stress: a review. Annals of Botany, 91, 179- 194.
  16. Cakmak, I. & Horst, W. (1991). Effect of aluminum on lipid peroxidation, superoxide dismutase, catalase and peroxidase activities in root tip of soybean (Glysin max). Plant Physiology, 83, 463-468.
  17. Castaneda, P. & Perez, L. M. (1996). Calcium ions promote the response of citrus lemon against fungal elicitors or wounding, Phytochemistry, 42, 595-598.
  18. Chance, B. & Maehly, A. C. (1955). Assay of catalases and peroxidases. Methods in Enzymology, 2, 764-775.
  19. Chang, Y. L., Kim, D. O., Lee, K. W., Lee, H. J. & Lee, C. Y. (2002). Vitamin C equivalent antioxidant capacity (VCEAC) of phenolic phytochemicals. Journal of Agricultural and Food Chemistry, 50(13), 3713-3717.
  20. Chengbin, X., Xuemei, L. & Lihong, Z. (2013). The effect of calcium chloride on growth, photosynthesis and antioxidant responses of (Zoysia japonica) under drought conditions. Plos One, 8(7), 1-10.
  21. Cousson, A. (2009). Involvement of phospholipase C-independent calcium-mediated abscisic acid signaling during Arabidopsis response to drought. Biologia Plantarum, 53, 53-62.
  22. Demiralay, M., Sağlam, A. & Kadıoğlu, A. (2013). Salicylic acid delays leaf rolling by inducing antioxidant enzymes & modulating osmoprotectant content in Ctenanthe setosa under osmotic stress. Turkish Journal of Biology, 37, 49-59.
  23. Drunasky, N. & Struve, D. K. (2005). Quercus macrocarpa and Q. prinus physiological and morphological response to drought stress. Urban Forestry & Urban Greening, 4, 13-22. 
  24. Dutilleul, C., Garmier, M., Noctor, G., Mathieua, C., Chetrita, P., Foyer, C. & Paepe, R. (2003). Leaf mitochondria modulate whole cell redox homeostasis set antioxidant capacity, and determine stress resistance through altered signaling and diurnal regulation. Plant cell, 15, 1212-1226.
  25. Ebrahimi, A., Naghavi, M. R. & Sabokdast, M. (2009). Evaluation and comparision of chlorophyll content, carotenoid, protein and enzyme in different barley species native in Iran. Journal of Agricultural Science, 40, 77-89. (in Farsi)
  26. Farooq, M., Wahid, A., Kobayashi, N., Fujita, D. & Basra, S. M. A. (2009). Plant drought stress: effects, mechanisms and management. Agronomy for Sustainable Development, 29, 185-212.
  27. Fujita, T., Maggio, A., Garcia-Rios, M., Stauffacher, C., Bressan, R. A. & Csonka, L. N. (2003). Identification of regions of the tomato γ-glutamyl kinase that are involved in allosteric regulation by proline. Journal of Biological Chemistry, 278(16), 14203-14210.
  28. Gue, Z., Ou, W., Lu, S. & Zhong, Q. (2006). Differential responses of antioxidative system to chilling and drought in four rice cultivars differing in sensitivity. Plant Physiology Biochemistry, 44, 828-836.
  29. Hanson, A. D. & Hitz, W. D. (1982). Metabolic responses of mesophytes to plant water deficits. Annual Review of plant Biology, 33, 163-203.
  30. Hassani, A. & Omid Beighi, R. (2002). Effects of water stress on some morphological, physiological and metabolical characteristics of basil (Ocimum basilicum). Agricultural Knowledge, 12 (3), 47-59.
  31. Hassanpour, H. & Niknam, V. (2014). Effect of drought stress on growth and activity of antioxidant enzymes of Mentha pulegium L. in flowering stage. Journal of Plant Physiology, 3 (8), 25-34. (in Farsi)
  32. Hernandez-Munoz, P., Almenar, E., Valle, V. D., Velez, D. & Gavara, R. (2008). Effect of chitosan coating combined with postharvest calcium treatment on strawberry (Fragaria ananassa) quality during refrigerated storage. Food Chemistry, 110, 428-435.
  33. Jafarzadeh, L., Omidi, H. & Bostani, A. A. (2013). Effect of drought stress and bio-fertilizer on flower yield, photosynthesis pigments and proline content of Marigold (Calendula officinalis L.). Iranian Journal of Medicinal and Aromatic Plants, 29(3), 666-680. (in Farsi)
  34. Jubany-Mari, T., Prinsen, E., Munne-Bosch, S. & Alegre, L. (2010). The timing of methyl jasmonate, hydrogen peroxidase and ascorbate accumulation during water deficit and subsequent recovery in the Mediterranean shrub (Cistus albidus L.). Journal Environmental and Experimental Botany, 69, 47-55.
  35. Kader, A. & Lindberg, S. (2010). Cytosolic calcium and pH signaling in plants under salinity stress. Plant Signaling and Behavior, 5, 233-238.
  36. Khazaie, H. R. & Borzooei, A. (2006). Effects of water stress on antioxidant activity and physiological characteristics of wheat. The first international conference on the theory and practices in Biological Water Saving (ICTPB), Beijing China.
  37. Kirnak, H., Tas, I., Kaya, C. & Higgs, D. (2002). Effects of deficit irrigation on growth, yield, and Fruit quality of eggplant under semi-arid conditions. Australian Journal of Agriculture Research, 53, 1367-1373.
  38. Koutinas, N., Sotiropoulos, T., Petridis, A., Almaliotis, D., Deligeorgis, E., Therios, I. & Voulgarakis, N. (2010). Effects of preharvest calcium foliar sprays on several fruit quality attributes and nutritional status of the Kiwifruit cultivar Tsechelidis. Horticultural Science, 45(6), 984-987.
  39. Křístková, E., Doležalová, I., Lebeda, A., Vinter, V. & Novotná, A. (2008). Description of morphological characters of lettuce (Lactuca sativa L.) genetic resources. Horticultural Science, 35, 113-129.
  40. Kurtyka, R., Małkowski, E., Kita, A. & Karcz, W. (2008). Effect of calcium and cadmium on growth and accumulation of cadmium, calcium, potassium and sodium in maize seedlings. Polish Journal of Environmental Studies, 17 (1), 51-56.
  41. Lonbani, M. & Arzani, A. (2011). Morpho-physiological traits associated with terminal drought-stress tolerance in triticale and wheat. Agronomy Research, 9, 315-329.
  42. Luna-Guzman, I. & Barrett, D. M. (2000). Comparison of calcium chloride and calcium lactate effectiveness in maintaining shelf stability and quality of fresh-cut cantaloupe. Postharvest Biology and Technology, 19, 61-72.
  43. Ma, Q. Q., Wang, W., Li, Y. H., Li, D. Q. & Zou, Q. (2006). Alleviation of photoinhibition in drought-stressed wheat (Triticum aestivum) by foliar-applied glycinebetaine. Journal of Plant Physiology, 163(2), 165-175.
  44. Ma, R., Zhang, M., Li, B., Dud, G., Wang, J. & Chen, J. (2005). The effects of exogenous Ca2+ on endogenous polyamine levels and drought-resistant traits of spring wheat grown under arid conditions. Journal of Arid Environments, 63, 177-190.
  45. Mani, F. (2014). Evaluation of drought stress on yield and physiological attributes in Cantaloupe crop (Cucumis melo L.). Indian Journal of Applied Research, 4, 6-10.
  46. Mansourifar, S., Shaban, M., Ghobadi, M. & Sabbaghpour, H. (2010). Physiological characteristics of Chickpea (Arietinum cicer L.) under drought stress and nitrogen fertilizer initiator. Iranian Journal of Pulses Research, 3(1), 53-66. (in Farsi)
  47. Masumi, A., Kafi, M., Khazaii, H. R., Davari, K. & Zare Mehrjerdi, M. (2010). Effect of drought stress on water condition and electrolyte leakage, photosynthesis and chlorophyll fluorescence at different growth stages of twonative masses of Kochia scoparia in salinity conditions. Iranian Journal of Field Crops Research, 10(3), 476-484. (in Farsi)
  48. Meda, A., Lamien, C. E., Romito, M., Millogo, J. & Nacoulma, O. G. (2005). Determination of the total phenolic, flavonoid and pralin contents in Burkina Fasan honey, as well as their scavenging activity. Food Chemistry, 91, 571-577.
  49. Miller, G., Suzuki, N. & Ciftci Yilmaz, S. (2010). Reactive oxygen species homeostasis and signaling during drought and salinity stresses. Plant Cell and Environment, 33, 453-467.
  50. Movahhedi Dehnavi, M., Ranjbar, M., Yadavi, A. R. & Kavusi, B. (2011). Effect of cycocel on proline, soluble sugares, protein, oil and fatty acids of flax (Linum usitatissimum M.) plants under drought stress in a pot trial Environ. Stresses in Crop Science, 3, 129-138. (in Farsi)
  51. Navvabpour, S., Mirkarimi, Sh. & Mazandarani, A. (2013). Evaluation of changes in enzymatic and non-enzymatic defense system of soybean cultivars in response to carcinogenic B carcinoma during growth stages. Crop Biotechnology, 5, 63-73. (in Farsi)
  52. Noctor, G. (2006). Metabolic signaling in defense and stress: the central roles of soluble redox couples. Plant Cell and Environment, 29, 409-425.
  53. Ozgen, U., Mavi, A., Terzi, Z., Yildirim, A., Coskun, M. & Houghton, P. J. (2006). Antioxidant properties of some medicinal Lamiaceae (Labiatae) species. Pharmaceutical Biology, 44, 107-112.
  54. Pandey, R. K., Maranville, J. W. & Admou, A. (2001). Tropical wheat response to irrigation and nitrogen in a Sahelian environment. I. Grain yield, yield components and water use efficiency. European Journal of Agronomy, 15, 93-105.
  55. Perucka, I. & Olszowka, K. (2010). Effect of calcium chloride treatment on the level of chlorogenic, β-carotene, lutein and tocopherols in lettuce (Lactuca sativa L.). Acta Agrobotanica, 64, 65-72.
  56. Perucka, I. & Olszowka, K. (2011). Accumulation of potassium, magnesium, calcium in fresh and cold stored leaves of lettuce (Lactuca sativa L.) after CaCl2 foliar treatment before harvest. The Elementary School Journal, 445- 454.
  57. Rimmer, D. L. (2006). Free radicals, antioxidants, and soil organic matter recalcitrance. European Journal of Soil Science, 57, 91-94.
  58. Sánchez-Madrigal, M. Á., Neder-Suárez, D., Quintero-Ramos, A., RuizGutiérrez, M. G., Meléndez-Pizarro, C. O., Piñón-Castillo, H. A., Galicia-García, T. & Ramírez-Wong, B. (2015). Physicochemical properties of frozen tortillas from nixtamalized maize flours enriched with β-glucans. Food Science and Technology, 35(3), 552-560.
  59. Sayyari, M., Ghavami, M., Ghanbari, F. & Kordi, S. (2013). Assessment of salicylic acid impacts on growth rate and some physiological parameters of lettuce plants under drought stress conditions. International Journal of Agriculture and Crop Science, 5(17), 1957-2013.
  60. Shen, X., Zhou, Y., Duan, L., Li, Z., Enej, A. E. & Li, J. (2010). Silicon effects on photosynthesis andantioxidant parameters of soybean seedlings under drought and ultraviolet-B radiation. Plant Physiology, 167, 1248-1252.
  61. Simsek, M. & Comlekcioglu, N. (2011). Effects of different irrigation regimes and nitrogen levels on yield and quality of melon (Cucumis melo L.). African Journal of Biotechnology, 10(49), 10009-10018.
  62. Soleimani Aghdam, M., Yousefpour, A. & Hassanpour, H. (2013). Enhancement of antioxidant capacity of cornelian cherry fruit by postharvest calcium treatment. Scientia Horticulture, 161, 160-164.
  63. Stepkowska, I. (2004). Porównanie dziaáania leczniczego Lactuca sp. (L) (Asteraceae) na podstawie medycyny dawnej, ludowej i wspóáczesnej. Post Fitoter, 4, 173–177.
  64. Sun, T., Powers, J. R. & Tang, J. (2007). Evaluation of the antioxidant activity of Asparagus, broccoli and their juices. Food Chemistry, 105, 101-106.
  65. Taiz, L. & Ezeiger, H. (2008). Plant Physiology. (2nd Ed.). Sinaye Associates Inc. Publisher. Sonderland Massachusetts. 757p.
  66. Tarigh Aleslami, M., Kafi, M., Nezami, A. & Zarghami, R. (2016). Interaction of cold and drought stress on changes in chlorophyll index, relative water content of leaves, electrolyte leakage and grain yield in three maize hybrid varieties. Journal of Crop Breeding, 9(23), 146-156. (in Farsi)
  67. Upadhyaya, H., Panda, S. K. & Dutta, B. K. (2011). CaCl2 improves post-drought recovery potential in (Camellia sinensis L. O. Kuntze). Plant Cell Reports, 30, 495-503.
  68. Vaziri, Z. H., Salamat, A., Ansari, M., Masihi, M., Heydari, N. & Dehghani sanich, H. (2008). Evapotranspiration plant (water consumption guidelines for plants) (Translation). Publications of the National Committee of Irrigation and Drainage, printing, Tehran. (in Farsi)