واکنش رقم ها و کلون های امیدبخش سیب زمینی به تنش اسمزی و دمایی در شرایط درون شیشه ای

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی سابق دکتری، دانشکده کشاورزی، دانشگاه تبریز، تبریز، ایران

2 دانشیار، دانشکده کشاورزی، دانشگاه تبریز، تبریز، ایران

3 استادیار، دانشکده کشاورزی، دانشگاه محقق اردبیلی، اردبیل، ایران

چکیده

به­منظور مطالعه واکنش گیاه سیب­زمینی در شرایط درون­شیشه­ای به تنش اسمزی و دمایی، آزمایشی به­صورت فاکتوریل در قالب طرح بلوک‌های کاملاً تصادفی با سه تکرار اجرا گردید. عامل اول چهار سطح تنش اسمزی ناشی از پلی­اتیلن­­گلیکول 6000 شامل شاهد، 5/0- ، 1- و 5/1-  مگاپاسکال به­ترتیب معادل صفر، 200 گرم در لیتر، 300 گرم در لیتر و 370 گرم در لیتر از ماده PEG-6000 بود و عامل دوم دما در سه سطح شامل 15، 25 و 35 درجه سانتی­گراد روی گیاهچه­های سه کلون امید­بخش 143، 301 و 306 و دو رقم تجاری پیکاسو و مارفونا در محیط کشت MS اعمال شدند. صفات فیزیولوژیکی و بیوشیمیایی مانند مقدار آب نسبی برگ (RWC)، میزان نشت الکترولیت (EL)، مقدار پرولین و فعالیت آنزیم­های کاتالاز (CAT)، پراکسیداز (POX) و اسکوربات­پراکسیداز (APX) و محتوی پروتئین اندازه­گیری شدند. نتایج تجزیه واریانس نشان داد که اثر ساده سه عامل و همچنین اثر متقابل عامل­های مورد مطالعه در سطح احتمال یک درصد بر صفات یادشده به‌استثنای محتوی پروتئین، تأثیر معنی­داری داشتند. مقایسه میانگین تیمارها نشان داد که با افزایش دما و مقدار پلی­اتیلن­گلیکول از میزان آب نسبی برگها کاسته شد، اما میزان نشت الکترولیت، مقدار پرولین، فعالیت آنزیم­های کاتالاز، پراکسیداز و آسکوربات­پراکسیداز افزایش داشتند. رقم مارفونا نسبت به شرایط تنش متحمل­ترین وکلون306 حساس­ترین بود، گیاهچه­های آن با افزایش شدت تنش از بین رفتند. تلفیق تنش اسمزی با افزایش درجه حرارت برای گیاهچه­های سیب­زمینی در شرایط درون­شیشه­ای قابل­تحمل نبود، به­طوری­که تنش اسمزی 5/0- مگاپاسکال به بالا و 35 درجه سانتی­گراد باعث خشک­شدن گیاهچه­ها گردیدند.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Response of potato’s cultivars and promising clones to osmotic and temperature stress under in vitro conditions

نویسندگان [English]

  • Farnush Jafari 1
  • Jaber Panahandeh 2
  • Ali Reza Motallebi-azar 2
  • Mousa Torabie-Giglou 3
1 Former Ph. D. Student, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
2 Associate Professor, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
3 Assistant Professor, Faculty of Agriculture and Natural Resources, University of Mohagege Ardabili, Ardabil, Iran
چکیده [English]

In order to study responses of potato plantlets under in vitro conditions to osmotic and temperature stresses, a factorial experiment was conducted in a completely randomized blocks design with three replications. The first factor was osmotic stress caused by polyethylene glycol 6000 in four levels including control, 0.5, 0.1 and -1.5 MPa which were equal to 0, 200, 300 and 370 grl-1 PEG. The second factor was temperature at three levels of 15, 25 and 35 °C applied on plantlets of three Promising clones (143, 301 and 306) and two commercial cultivars (Picasso and Marfona) in MS medium. Physiological and biochemical traits including relative water content (RWC), electrolyte leakage (EL), proline, catalase (CAT), peroxidase (POX) and ascorbate peroxidase (APX) as well as protein content were measured. Results of variance analysis showed that the simple effects of three factors as well as the interaction were significant at 1≤0.01 on the mentioned traits, but the protein content was not significant. Comparing the mean of treatments showed that by increasing the temperature and concentrations of PEG, RWC was reduced, while EL, proline content, CAT, POX and APX enzymes activity were increased. Also, results indicated that Marfona was more tolerant to stress conditions than other varieties and clones, which seems is due to preventing the effects of stress by increasing the accumulation of proline and the activity of antioxidant enzymes. Clone 306 was more susceptible than other varieties and clones and showed more vulnerability to stress and its plantlets were killed by increasing stress levels. The combination of osmotic and temperature stresses were not tolerated by potato’s plantlets under in vitro conditions, because osmotic stress of -0.5 MPa and higher in combined with 35°C caused the plantlets to dry.

کلیدواژه‌ها [English]

  • Catalase
  • PEG
  • Peroxidase
  • potato
  • proline
  • temperature

مراجع

  1. Aebi, H. (1984). Catalase in vitro. Methods in Enzymology, 105, 121-126.
  2. Allagulova, C. R., Gilamov, F. R., Shakirova, F. M. & Vakhitov, V. A. (2003). The plant dehydrins: structure and functions. Biochemistry, 68, 945-951.
  3. Allen, E. J. & Scott, R. K. (1998). Principles of agronomy and their application in the potato industry. In: Harris, P. (Ed.), Potato crop. Chapman and Hall Ltd, London, 816-882.
  4. Amini, F., Askari, M. & Haghir, M. (2017). Changes in protein and antioxidant system of Cucumis sativus cv. Isfahani in response to drought stress. Journal of Cell & Tissue, 7(4), 375-385. (in Farsi)
  5. Amini, S., Ghobadi, C. & Yamchi, A. (2015). Proline accumulation and osmotic stress: an overview of P5CS gene in plants. Journal of Plant Molecular Breeding, 3, 44-55
  6. Anjum, N. A., Arena, C. & Singhgill, S. (2014), Reactive Oxygen species and response of antioxidants as ROSscavengers during enviromental stress in plant. Frontiers in Enviromental Science, 2, 1-13.
  7. Ariano, S., Bartolomeo, D., Cristos, X. & Andras, M. (2005). Antioxidant defenses in olive trees during drought stress: changes in activity of some antioxidant enzmes. Functional Plant Biology, 32, 45-53.
  8. Ashraf, M. & Iram, A. (2005). Drought stress induced changes in some organic substances in nodules and other plant parts of two potential legumes differing in salt tolerance. Flora, 200, 535-546.
  9. Bates, L. S., Waldern, R. P. & Tears, I. D. (1973). Rapid determination of free proline for water-stress studies. Plant and Soil, 39, 205-207.
  10. Boroujerdnia, M., Bihamta, M., AlamiSaid, K. & Abdossi, V. (2016). Effect of drought tension on proline content, soluble carbohydrates, electrolytes leakage and relative water content of bean (Phaseolus vulgaris L.). Crop Physiology Journal, 8(29), 23-41. (in Farsi)
  11. Bradford, M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72 (1-2), 248-254.
  12. Carr, M. K. V. (1989). Potato quality control with irrigation. Water Irrigation Review, 9, 28-29.
  13. Chance, B. & Maehly, A. (1955). Assay of catalase and peroxidase. Methods Enzymology, 2, 764-817.
  14. Cook, D., Fowler, S., Fiehnand, O. & Etval, A. (2004). Prominent role for the CBF cold response pathway in configuring the low temperature metabolome of arabidopsis. Plant Biology, 101, 15243-8.
  15. Dong, X., Bi, H., Wu, G. & Ai, X. (2013). Drought induced chilling tolerance in cucumber involves membrane stabilization improved by antioxidant system. International Journal of Plant Production, 7 (1), 67-80.
  16. Foryer, C. & Noctor, G. (2000), Oxygen processing in photosynthesis: regulation and signaling. New Phytologist, 146, 359-388.
  17. Ganjeali, A., Rahbarian, R., Bagheri, A. R. & Malekzadeh-Shafaroudi, S. (2014). Study on drought stress effects on morphological, physiological and biochemical characteristics of chickpea genotypes (Cicer arietinum L.) under field condition. Iranian Journal of Pulses Research, 5(1), 91-102. (in Farsi)
  18. Gholipour, S. & Ebadie, A. (2017). Study change compatibility metabolites and antioxidant enzyme activities of wheat cultivars under water stress. Journal of Process and Function, 6 (19), 219-232.
    (in Farsi)
  19. Golen, H., Turhan, E. & Eris, A. (2006). Changes in peroxidase activities and soluble proteins in strawberry varieties under salt stress. Acta Physiologiae Plantarum, 28, 109-116.
  20. Habibi, G. (2013). Effect of drought stress and selenium spraying on photosynthesis and antioxidant activity of spring barley. Acta Agriculturae. Slovenica, 101 (1), 31-39.
  21. Hajihosseinlo, N., Hosseini Sargein, S. & Jamei, R. (2016). The study of interactive effects of UV-B Radiation and drought stress on some physiological traits of two cultivar of gourd (Cucurbita pepo L.). Iranian Journal of Plant Physiology and Biochemistry, 1(2), 16-26. (in Farsi)
  22. Hassandokht, M. R., Bigdelo, M., Soltani, F. & Salehi Mohammadi, R. (2017). Evaluation of drought stress tolerance and its effects on growth characteristics in some accessions of colocynth (Citrullus colocynthis (L.) Schrad). Iranian Journal of Horticultural Science, 48(2), 275-284. (in Farsi)
  23. Hassani Moghadam, E., Esna-Ashari, M. & Rezainejad, A. H. (2015). Effect of Drought Stress on Some Physiological Characteristics in Six Commercial Iranian Pomegranate (Punica granatum L.) Cultivars. Plant Production Technology, 7(1), 1-11. (in Farsi)
  24. Hokmabadi, H., Noorian Bigdeli, S., Narimani, R. & Moghaddam, M. (2017). Study of beet bagasse effect on biochemical characteristics, photosynthetic pigments and antioxidant activity of leaf extracts of peppermint (Mentha piperita L.) under drought stress conditions. Journal of Science and Technology of Greenhouse Culture, 8 (1), 135-145.
  25. Hosseini Boldaji, S. A., Khavari-Nejad, R. A., Hassan Sajedi, R., Fahimi, H. & Saadatmand, S. (2012) Water availability effects on antioxidant enzyme activities lipid peroxidation, and reducing sugar contents of alfalfa (Medicago sativa L.). Acta Physiologia Plantarum, 34, 1177-1186.
  26. Javadi, T., Arzani, K. & Ebrahimzadeh, H. (2004). Evaluation of soluble carbohydrate content and proline in 9 genotypes of Asian pear (Pyrus serotina Rehd) under drought stress. Iranian Journal of Plant Biology, 17(3), 369-378. (in Farsi)
  27. Kakulvand, R., Fallah, S. & Abassi Sourki, A. (2017), Effects of species competition on photosynthetic pigments, prolin relative water content, and essence fenugreek (Trigonella foenum graceum) and black cumin (Nigella sativa L.) under drought stress conditions in intercropping system. Journal of process and Function, 6 (19), 255-270. (in Farsi)
  28. Karimi, S., Yadollahi, A. & Arzani, K. (2013). Response of Almond Genotypes to Osmotic Stress Induced In Vitro. Journal of Nuts, 4(4), 1-7.
  29. Laspina, N. V., Groppa, M. D., Tomaro, M. L. & Benavides, M. P. (2005). Nitric oxide protects sunflower leaves against Cd-induced oxidative stress. Plant Science, 169, 323-330.
  30. Liu, X. & Huang, B. (2000). Heat stress injury in relation to membrane lipid peroxidation in creeping bentgrass. Crop Science, 40, 503-510.
  31. Lutts, S., Kinet, J. M. & Bouharmont, J. (1995). Changes in plant response to NaCl during development of rice (Oryza sativa L.) varieties differing in salinity resistance. Journal of Experimental Botany, 46 (12), 1843-1852.
  32. Mafakheri, A., Siosemardeh, A., Bahramnejad, B. & Struik, P. C. (2011). Effect of drought stress and subsequent recovery on protein, carbohydrate contents, catalase and peroxidase activities in three chickpea (Cicer arietinum) cultivars. Australian Journal of Crop Science, 5 (10), 1255-1260.
  33. Maghsoodi, M., Razmjoo, J. & Gheysari, M. (2016).  Effect of drought on biochemical properties of root and shoot of alfalfa cultivars (Medicago sativa L.). Aridbiom Gournal, 6(1), 31-43.(in Farsi)
  34. Masoudi Sadaghiani, F. & Amini Dehaghi, M. (2016). Changes in Some Osmolytes and Potosynthetic Pigments Accumulation and Antioxidant Enzymes Activity in Potato under Water Stress in Greenhouse Conditions. Iranian Journal of Horticultural Science and Technology, 17 (1), 29-38. (in Farsi)
  35.  Mehri, H. R., Ghobadi, S., Baninasab, B., Ehsanzadeh, P. & Gholami, M. (2015). Evaluation of some physiological and morphological responses of four Iranian grapevine (Vitis vinifera L.) cultivars to drought stress under in vitro conditions. Journal of Plant Process and Function, 3(10), 115-126. (in Farsi)
  36. Michel, B. E. & Kaufmann, M. R. (1973). The osmotic potential of polyethylene glycol 6000. Plant Physiologym 51, 914-916.
  37. Mittler, R. (2002). Oxidative stress, antioxidant and stress tolerance. Annual Review of Plant Science, 7, 405-415
  38. Mohsenzade, S., Malboobi, M.A., Razavi, K. & Farrahi Aschtiani, S. (2006). Physiological and molecular responses of Aeluropus lagopoides to water deficit. Environmental and Experimental Botany, 56, 374-322
  39. Molaahmad Nalousi, A., Hatamzadeh, A., Ghasemnezhad, M. & Alibiglouei, M. H. (2015). The study of physiological and biochemical responses of Agrostis stolonifera and Festuca arundinacea Schreb. under drought stress. Iranian Journal of Plant Biology, 6(22), 105-116. (in Farsi)
  40. Moussa, H. & Abdel-Aziz, S. M. (2008). Comparative response of drought tolerant and drought sensitive maize genotypes to water stress. Australian Journal of Crop Science, 1, 31-36.
  41. Najaf Zadeh, S. & Ehsanpour, A. (2012), Effect of drought stress on some physiological parameters of two potato cultivars (Kenebec and Concord) under in vitro culture condition. Journal of Aridbiom, 2(1), 70-82. (in Farsi)
  42. Nakano, Y. & Asada, K. (1987). Purification of ascorbate peroxidase in spinach chloroplast in inactivation in ascorbate-depleted medium and reactivation by monodehydro ascorbate radical. Plant Cell Physiology, 28, 131-133.
  43. Nasibi, F. (2011). Effect of different concentrations of sodium nitroprusside (SNP) pretreatment on oxidative damages induced by drought stress in tomato plant. Iranian Journal of Plant Biology, 3(9), 63-74. (in Farsi)
  44. Nouri, A., Nezami, A., Kafi, M. & Hassanpanah, D. (2016), Evaluation of Water Deficit Tolerance of 10 Potato Cultivars Based on some Physiological Traits and (Solanum tuberosum L.) Tuber Yield in Ardabil Region. Journal of Crop Ecophysiology, 19(1), 243-268. (in Farsi)
  45. Rabbi Angourani, H., PanahandehYengejeh, J., Boland Nazar, S. A., Saba, J. & Zare Nahandi, F. (2016). The effect of drought stress and salicylic acid on some morpho-physiological and phytochemistry characteristics of medicinal pumpkin (Cucurbita pepo var. styriaca). Ph.D. dissertation, Horticultural science, Tabriz University.
  46. Ratnayaka, H. H., Molin, W. T. & Sterling, T. M. (2003). Physiological and antioxidant responses of cotton and spurred anoda under interference and mild drought. Journal of Experimental Botany, 54, 2293-2305.
  47. Razavizadeh, R. & Shahriari, E. (2017). Comparative study of drought stress effects on physiological and defense parameters of some varieties of sorghum under In vitro culture. Journal of Process and Function, 6 (19), 163-180. (in Farsi)
  48. Sairam, R. K. & Srivastava, G. C. (2002). Changes in antioxidant activity in sub-cellular fractions of tolerant and susceptible wheat genotypes in response to long term salt stress. Plant Science, 162, 897-904.
  49. Sanchez-Rodriguez, E., Rubio-Wilhelmi, M., Cervilla, L. M., Blasco, B., Rios, J. J., Rosale, M. A. S., Romero, L. & Ruiz, J. M. (2010). Genotypic differences in some physiological parameters symptomatic for oxidative stress under moderate drought in tomato plants. Plant Science, 178, 30-40.
  50. Sariri, R., Galvani, M., Fotouhi Ghazvini, R. & Jafarian, V. (2011). The effect of cold temperature stress on antifreeze protein production and lipid peroxidation in two citrus species. Iranian Journal of Plant Biology, 3(7), 97-102. (in Farsi)
  51. Sharma, P. & Dubey, R. S. (2005). Drought induces oxidative stress and enhances the activities of antioxidant enzymes in growing rice seedlings. Plant Growth Regulation, 46, 209-221.
  52. Sinclair, T. R. & Ludlow, M. M. (1985). Who taught plants the thermodynamics of water? The unfulfilled potential of plant water potential. Australian Journal of Plant Physiology, 12, 213-217.
  53. Smart, R. E. & Bingham, G. E. (1974). Rapid estimates of relative water content. Plant Physiology, 53(2), 258-260.
  54. Soukhtesaraee, R., Ebadi, A., Salami, S. A. & Lesani, H. (2017). Evaluation of oxidative parameters in three grapevine cultivars under drought stress. Iranian Journal of Horticultural Science, 48(1), 85-98. (in Farsi)
  55. Sreenivasulu, N., Grimm, B., Wobns, U. & Weschke, W. (2005). Asymmetrical leaves induced by water deficit show asymmetrical photosynthesis in common bean. Brazilian Journal of Plant Physiology, 17(2), 223-227.
  56. Tawfik, K. M. (2008). Effect of water stress in addition to potassiom application on mung bean. Australian Journal of Basic and Applied Sciences, 2, 42-52.
  57. Vendruscolo, A. C. G., Schuster, I., Pileggi, M., Scapim, C. A., Molinari, H. B. C., Marur, C. J. & Vieira, L. G. C. (2007). Stress-induced synthesis of prolineconfers tolerance to water deficit intransgenic wheat. Journal of Plant Physiology, 164, 1367-1376.
  58. Yadav, R. S. & Bhushan, C. (2001). Effect of moisture stress on growth and yield in rice genotype. Indian Journal of Agricultural Research, 2, 104-107.
  59.  Zarrabi, M. M., Talaai, A., Soleimani, A. & Haddad, R. (2010), Physiological Characteristic and Biochemical Changes Six Olive (Olea europaea L.) Cultivars under Drought Stress. Journal of Horticultural Science, 24(2), 234-244. (in Farsi)
  60. Zokaee Khosro Shahi, M. R., Esna Ashari, M., Ershadi, A. & Imani, A. (2014). Physiological responses of five almond Species to PEG-induced drought stress. Plant Production Technology, 5(2), 73-88. (in Farsi)

 

علوم باغبانی ایران
دوره 50، شماره 3
آذر 1398
صفحه 633-648

فایل ها

سابقه مقاله

  • تاریخ دریافت: 27 اردیبهشت 1397
  • تاریخ بازنگری: 12 تیر 1397
  • تاریخ پذیرش: 23 تیر 1397

هم رسانی

ارجاع به این مقاله

آمار