اثر تابش اشعه فرابنفش C بر برخی ترکیبات فیتوشیمیایی و فعالیت پاداکسایشی در پوست حبه انگور رقم بیدانه سفید

نوع مقاله: مقاله کامل

نویسندگان

1 دانشجوی دکتری فیزیولوژی و اصلاح درختان میوه، گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه بوعلی سینا، همدان، ایران

2 استاد، گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه بوعلی سینا، همدان، ایران

چکیده

اثر پرتو فرابنفش نوع C (UV-C) به­تنهایی یا در ترکیب با دیگر تیمارها بر سنتز ترکیبات ثانویه در گیاهان، اخیرا مورد توجه قرار گرفته است. در این پژوهش، اثر پرتو UV-C بر برخی ویژگی‌های فیتوشیمایی و فعالیت برخی آنزیم‌های دخیل در متابولیسم ترکیبات فنلی در انگور مورد بررسی قرار گرفت. ابتدا حبه‌های انگور رقم بیدانهسفید در معرض پرتو UV-C در شدت­های0 (شاهد)، 8/0، 2/1 و 2/4 کیلوژول بر مترمربع قرار گرفته و سپس در دمای 15 درجه سانتی‌گراد به­مدت 6 روز نگهداری شدند. شدت­های کم پرتو فرابنفش، تجمع فنل کل در پوست حبه را القا کرد. کمترین میزان فلاونوئید کل در حبه­های تیمار شده با بالاترین شدتUV-C  (kJm-2 2/4) در روز اول مشاهده شد درحالی­که حبه‌های تیمارشده با شدت­ کم UV-C (kJm-2 8/0) پس از گذشت 5 روز بیشترین میزان فلاونوئید را داشتند (45/0 میلی­گرم روتین در گرم وزن تر). همچنین کاربرد تابش UV-C منجر به افزایش ظرفیت پاداکسایشی نمونه­های تیمارشده در مقایسه با شاهد گردید. لازم به ذکر است پرتوتابی باUV-C  اثر معنی­داری بر مقدار تانن کل پوست حبه نداشت؛ با این وجود اثر UV-C بر فعالیت آنزیم فنیل­آلانین­آمونیالیاز (PAL) در طول مدت نگهداری قابل­توجه بود. UV-C فعالیت آنزیم پراکسیداز (POD) را تا اوسط مدت­ نگهداری کنترل کرد؛ ولی در مورد آنزیم پلی‌فنل‌اکسیداز (PPO) این رفتار وابسته به شدت UV-C بود. نتایج این پژوهش نشان داد که کاربرد UV-C در شدت­های پایین، روش مناسبی برای افزایش میزان ترکیبات فنلی و ظرفیت پاداکسایشی انگور تازه­خوری رقم بیدانهسفید در طول مدت نگهداری کوتاه می­باشد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Influence of UV-C irradiation on some phytochemicals and antioxidant activities in ‘Sultana’ table grape skin

نویسندگان [English]

  • Ehsan Ranjbaran 1
  • Mansour Gholami 2
1 Ph. D. Candidate, Department of Horticultural Science, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran
2 Professor, Department of Horticultural Science, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran
چکیده [English]

The effect of UV-C irradiation, either alone or in combination with other treatments, on the biosynthesis of secondary compounds in plants has been recently become a matter of interest. In this study, the influence of UV-C light on some phytochemical traits and activity of enzymes involved in phenolic metabolism in grape was investigated. At first, the berries of table grape cv. Sultana were subjected to UV-C irradiation in different doses of 0 (control), 0.8, 1.2, and 4.2 kJm-2, then they were kept at 15°C for 6 days. UV-C low doses induced total phenol in the berry skin. The lowest flavonoid content was observed in berries subjected to the highest dose of UV-C (4.2 kJm-2) on the first day, whereas those irradiated by the low dose of UV-C (0.8 kJm-2) had the most flavonoid content after 5 days (0.45 mg Rutin/g FW). Also, UV-C application improved antioxidant capacity of treated samples in comparison with control. It should be noted that UV-C irradiation had no effect on total tannin content of the berry skin. However, the effect of UV-C radiation on phenylalanine amonialyase (PAL) activity was remarkable during storage. The UV-C restricted the POD activity up to the middle of storage. However, in terms of polyphenol oxidase (PPO), this behavior was dosage-dependent. The results showed that UV-C application at the low doses is an appropriate way to increase phenolic content and antioxidant capacity of table grapes cv. Sultana during limited storage time.

کلیدواژه‌ها [English]

  • Enzyme activity
  • irradiation
  • phenolic compound
  • Vitis vinifera
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