تأثیر محیط‌های کشت و تنظیم‌کننده‌های مختلف بر استقرار درون‌شیشه‌ای و تولید متابولیت‌های ثانویه گیاه Vaccinium arctostaphylos L.

نوع مقاله: مقاله کامل

نویسندگان

1 دانشجوی کارشناسی ارشد، دانشکده کشاورزی و منابع طبیعی، دانشگاه محقق اردبیلی، اردبیل

2 دانشیار، دانشکده کشاورزی و منابع طبیعی، دانشگاه محقق اردبیلی، اردبیل

3 استاد، دانشکده کشاورزی و منابع طبیعی، دانشگاه محقق اردبیلی، اردبیل

4 استادیار، دانشکده کشاورزی و منابع طبیعی، دانشگاه محقق اردبیلی، اردبیل

چکیده

این تحقیق به­منظور بررسی تأثیر نوع محیط کشت پایه و تنظیم­کننده­های رشد گیاهی مختلف بر استقرار و رشد ریزنمونه­های قره­قاط و میزان متابولیت‌های ثانویه در شرایط کشت درون­شیشه­ای انجام گردید. برای این منظور، ریزنمونه‌های تک­‌گره­ای و جوانه­های انتهایی قره­قاط از مناطق جنگلی استان اردبیل تهیه و از طریق تیمار با محلول g/l3 بنومیل، محلول H2O2 (5%)­، اتانول 70% و در نهایت محلول هیپوکلریت­سدیم 5/2% با 10pH=  به­مدت 12دقیقه به ترتیب ضدعفونی شدند. ریزنمونه­ها روی محیط کشت­های MS، AN و WPM حاوی  mg/l­1/0 NAA یا IBA و سطوح مختلف BAP، زآتین و TDZ کشت شدند. نتایج نشان داد که درصد ریزنمونه‌های رشد کرده و تعداد برگ در هر ریزنمونه به‌طور معنی‌داری تحت تأثیر نوع محیط کشت پایه، ترکیب هورمونی محیط کشت و اثر متقابل بین آنها قرار گرفت. تعداد برگ در هر ریزنمونه و رشد ریزنمونه‌ها در  محیط کشت MS به‌طور معنی‌داری بیشتر از محیط کشت­های پایه AN و WPM بود. بیشترین درصد ریزنمونه‌های رشد کرده و تعداد برگ در هر ریزنمونه در محیط کشت پایه MS حاوی (mg/l 1/0) IBA به­همراه 5/0 و 1 میلی‌گرم بر لیتر زآتین یا 5/0 میلی‌گرم بر لیتر BAP به­دست آمد.­همچنین از نظر میزان متابولیت‌های ثانویه بین تیمار­های مختلف اختلاف معنی­داری وجود داشت. بیشترین میزان ترکیبات آنتوسیانینی و فلاونوئید به­ترتیب در محیط کشت MS حاوی 2 میلی­گرم بر­لیتر BAP و 1/0 میلی­گرم بر­لیتر IBA و MS حاوی 2 میلی­گرم بر لیتر TDZ و 1/0 میلی­گرم بر لیتر NAA به­دست آمد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Effect of culture media and plant growth regulators on in vitro growth and production of secondary metabolites in Vaccinium arctostaphylos L.

نویسندگان [English]

  • Mehran Noruzpour 1
  • Nasser Zare 2
  • Rasool Asghari 3
  • Parisa Sheikhzadeh 4
1 M.Sc. Student, Department of Agronomy and Plant Breeding, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran
2 Associate Professor, Department of Agronomy and Plant Breeding, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran
3 Professor, Department of Agronomy and Plant Breeding, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran
4 Assistant Professor, Department of Agronomy and Plant Breeding, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran
چکیده [English]

The current study was conducted to evaluate the effect of basal culture media and plant growth regulators on establishment and growth of Whortleberry explants and production of secondary metabolites in in vitro conditions. Accordingly, single node explants and apical buds of Whortleberry were collected from forestry areas (Soha, Ardabil, Iran), surface sterilized by benomyl (3 g/l), H2O2 (5%), Ethanol (70%) and sodium hypochlorite (2.5%, pH=10) for 12 min, respectively. The explants were cultured on MS, AN, and WPM media supplemented with 0.1 mg/l NAA or IBA, and different levels of BAP, Zeatin and TDZ. Results showed that growth of explants (%) and number of leaves per explant were affected significantly by type of basal culture medium, plant growth regulators combination and their interaction. Number of leaves per explant and explant’s growth in MS medium was significantly higher than in AN, and WPM media. The highest shoots (%) and number of leaves were obtained in MS basal medium supplemented with 0.1 mg/l IBA plus 0.5 and 1 mg/l Zeatin or 0.5 mg/l BAP. Moreover, there were significant differences among the treatments for secondary metabolite contents. The highest amount of anthocyanin and flavonoid were obtained in MS medium supplemented with 2 mg/l BAP+ 0.1 mg/l IBA and MS medium plus 2 mg/l TDZ and 0.1 mg/l NAA.

کلیدواژه‌ها [English]

  • Basal MS medium
  • Caucasian whortleberry
  • In vitro micro-propagation
  • flavonoids
  • Medicinal plant
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