اثر پرولین و اسید سالیسیلیک بر شاخص‌های مورفولوژیکی و فیتوشیمیایی استویا (Stevia rebaudiana Bert.)

نوع مقاله: مقاله کامل

نویسندگان

1 دانشجوی سابق کارشناسی ارشد، دانشکده کشاورزی، دانشگاه زنجان، زنجان، ایران

2 استادیار، دانشکده کشاورزی، دانشگاه زنجان، زنجان، ایران

چکیده

در این مطالعه به­منظور بررسی اثر پرولین و اسید سالیسیلیک بر صفات مورفولوژیکی و فیتوشیمیایی گیاه استویا آزمایشی به­صورت طرح فاکتوریل در قالب بلوک­های کامل تصادفی با سه تکرار در مزرعه تحقیقاتی دانشگاه زنجان اجرا شد. تیمارهای آزمایش شامل پرولین در سه سطح (صفر، 1، 2 میلی­مولار) و اسید سالیسیلیک در سه سطح (صفر، 3/0، 6/0 میلی­مولار) بودند که به­صورت محلول‌پاشی برگی پس از استقرار نشا، در سه مرحله به فاصله 10 روز انجام شد. صفات مورد بررسی شامل سطح برگ، عملکرد وزن تر و خشک برگ و کل بوته در واحد سطح، کلروفیل (a،b و کل)، کاروتنوئید، فنل کل، فلاونوئید کل و درصد فعالیت آنتی­اکسیدانی بودند. تیمارها به­طور قابل­توجهی در صفات بالا نسبت به شاهد تأثیر­گذار بودند. بالاترین میانگین سطح برگ (cm239/5949)، عملکرد تر (g/m217/787) و خشک برگ (g/m25/228) و عملکرد تر (g/m223/1339) و خشک کل بوته (g/m252/327) در سطح تیمار 1 میلی­مولار پرولین به­همراه 3/0 میلی­مولار اسید سالیسیلیک مشاهده شد. کاربرد اثرات ساده غلظت­های 2 میلی­مولار پرولین و 6/0 میلی‌مولار اسید سالیسیلیک منجر به افزایش میزان کلروفیل a، b، کاروتنوئید و فلاونوئید کل شد. بالاترین میزان کلروفیل کل (mg/g fw23/5) در اثرات متقابل تیمار 2 میلی­مولار پرولین به­همراه 3/0 میلی­مولار اسید سالیسیلیک و بالاترین میزان فنل (mg/g dw94/19) در 1 میلی­مولار پرولین به­همراه 6/0 میلی­مولار اسید سالیسیلیک مشاهده شد. می­توان استنباط نمود که تیمار با پرولین و اسید سالیسیلیک با تأثیر بر فرآیندهای رشدی و فیزیولوژیکی، در افزایش عملکرد و تولید متابولیت­های ثانویه استویا مؤثر بودند.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Effect of proline and salicylic acid on morphological and phytochemical characteristics of Stevia rebaudiana (Bert.)

نویسندگان [English]

  • Arefeh Rastgoo 1
  • Mohsen Sanikhani 2
  • Azizollah Kheiry 2
  • Mitra Aelaei 2
1 Former M.Sc. Student, Faculty of Agriculture, University of Zanjan, Zanjan, Iran
2 Assistant Professor, Faculty of Agriculture, University of Zanjan, Zanjan, Iran
چکیده [English]

To study the effect of foliar application of proline and salicylic acid on growth, morphological and phytochemical traits of Stevia, an experiment based on completely randomized block design in a factorial arrangement was conducted with three replications in the University of Zanjan. Foliar applications of proline in three levels (0, 1, 2 mM) as well as salicylic acid (0, 0.3, 0.6 mM) were applied after establishment of transplants in the field three times with 10 days intervals. Factors including leaf area, leaf fresh and dry weight, total fresh and dry weight, chlorophyll a, b and total chlorophyll, carotenoid, total phenol, total flavonoid and antioxidant activity were studied. Results showed that salicylic acid and proline applications significantly influenced above mentioned traits. The highest average leaf area/ plant (5949.39 cm2), leaf fresh (787.17 g/m2) and dry leaf weight (228.5 g/m2), total fresh (1339.23 g/m2) and total dry weight (327.52 g/m2) were recorded in 1 mM proline in combination with 0.3 mM salicylic acid. Solely applications of proline at 2 mM and salicylic acid at 0.6 mM enhanced chlorophyll a, b, carotenoid and total flavonoid contents. The highest average total chlorophyll (5.23 mg/g fw) observed in 2 mM proline in combination with 0.3 mM salicylic acid and highest phenol (19.94 mg/g dw) recorded in 1 mM proline in combination with 0.6 mM salicylic acid. Collectively, results showed that application of proline and salicylic acid significantly improved vegetative growth and production of active substances in Stevia rebaudiana.

کلیدواژه‌ها [English]

  • Active substances
  • Amino acid
  • Chlorophyll
  • Salicylic Acid
  • yield
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