تأثیر نانونقره بر آلودگی باکتریایی و شاخص‌های مورفولوژیکی و بیوشیمیایی بادام GN15 کشت بافتی

نوع مقاله: مقاله کامل

نویسندگان

1 دانشجوی سابق دکتری، دانشکده کشاورزی، دانشگاه محقق اردبیلی، اردبیل، ایران

2 دانشیار، دانشکده کشاورزی، دانشگاه محقق اردبیلی، اردبیل، ایران

3 دانشیار، دانشکده کشاورزی و منابع طبیعی دانشگاه ارومیه، ارومیه، ایران

چکیده

نانوتکنولوژی توانسته راهگشای روش­های نوینی در علوم گیاهی و تحقیقات کشاورزی باشد. این تحقیق به­منظور بررسی اثرات نانونقره در 4 غلظت (صفر (شاهد)، 100، 150 و 200 میلی­گرم بر لیتر) و 8 تکرار، در قالب طرح بلوک­های کامل تصادفی، بر درصد زنده­مانی، آلودگی­ باکتریایی و شاخص­های مورفولوژیکی و بیوشیمیایی ریزنمونه­های بادام GN15، در دانشگاه ارومیه، اجرا شد. جوانه انتهایی بادام رشدیافته در محیط کشت MS حاوی 3 درصد ساکاروز و 8/0 درصد آگار، در محیط مشابه، حاوی غلظت­های مختلف نانونقره و 1 میلی­گرم بر لیتر BAP، واکشت و در اتاقک رشد قرار داده شدند. با افزایش غلظت نانونقره، درصد زنده­مانی ریزنمونه­ها افزایش و آلودگی باکتریایی و قارچی کاهش یافتند. نانونقره در غلظت­های صفر تا 100 میلی­گرم بر لیتر، باعث افزایش طول ریشه، تعداد برگ، میزان کلروفیل a، b، کلروفیل کل، کارتنوئید و کربوهیدرات­های محلول برگ و در غلظت­های بالاتر، موجب کاهش این فاکتورها شد. افزایش غلظت نانونقره باعث کاهش طول ساقه و تعداد ساقه شد، اما روی تعداد ریشه تأثیر معنی­داری نداشت. با وجود تأثیر افزایش غلظت نانونقره در کاهش آلودگی باکتریایی، تا سطح 200 میلی­گرم بر لیتر، حتی تا کمتر از 10 درصد، بهترین سطح، درجهت بهبود ویژگی­های رویشی و بیوشیمیایی ریزنمونه­های GN15، تیمار 100 میلی­گرم بر لیتر بود.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Effects of Nano silver on bacterial contamination and morphological and biochemical indices of in vitro GN15 almond rootstock

نویسندگان [English]

  • Elnaz Hatami 1
  • Ali akbar Shokouhian 2
  • Alireza Ghanbari 2
  • Lotfali Naseri 3
1 Former Ph.D. Student, Faculty of Agriculture, University of Mohaghegh Ardabili, Ardabil, Iran
2 Associate Professor, Faculty of Agriculture, University of Mohaghegh Ardabili, Ardabil, Iran
3 Associate Professor, Faculty of agriculture, Urmia University, Urmia, Iran
چکیده [English]

Nanotechnology has been able to pave the way for new methods in plant sciences and agricultural research. This research was carried out to investigate effects of Nanosilver in 4 concentrations (0 (control), 100, 150 and 200 mg L-1) at 8 replications in a complete randomized block design, on viability, bacterial contamination and some morphological and biochemical indices of GN15 explants at Urmia University. Shoot tip of cultivated GN15 rootstock were placed in MS medium containing 3% sucrose and 0.8% agar and different concentrations of Nanosilver and 1 mg L-1 BAP. Samples were recultured and were grown in growth chamber. By increasing Nanosilver concentration, the plants survival increased and the percentage of bacterial and fungal contamination decreased. Nanosilver increased the root length, leaf number, chlorophyll a, b, total chlorophyll, carotenoids and soluble carbohydrates at concentrations from 0 to 100 mg L-1. At higher concentrations, these factors were decreased. Increasing the concentration of Nanosilver decreased the shoot number and lateral branches number of explants, but did not have a significant effect on root number. Despite the effect of increasing the concentration of Nano silver on reducing bacterial contamination, up to 200 mg L-1, even to less than 10%, the best level, improving the biotic properties of GN15 explants was 100 mg L-1.

کلیدواژه‌ها [English]

  • Carotenoid
  • Chlorophyll
  • Contamination
  • Nanotechnology
  • Root length
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