تأثیر اسید‌سالیسیلیک و متیل‌جاسمونات بر ویژگی‌های مورفولوژیکی، فعالیت آنزیمی‌های آنتی‌اکسیدانی و درصد اسانس گیاه سرخارگل (Echinacea purpurea L.) در شرایط اقلیمی زنجان

نوع مقاله: مقاله کامل

نویسندگان

1 دانشجوی سابق کارشناسی ارشد، دانشکده کشاورزی، دانشگاه زنجان

2 استادیار، دانشکده کشاورزی، دانشگاه زنجان

چکیده

به­­منظور ارزیابی اثر اسید­سالیسیلیک و متیل­جاسمونات و اثرات متقابل آنها بر برخی از ویژگی­های گیاه سرخارگل، آزمایشی به­صورت فاکتوریل بر پایه طرح بلوک­های کامل تصادفی در سه تکرار، در شرایط اقلیمی زنجان اجرا شد. تیمارهای آزمایش شامل سه سطح اسیدسالیسیلیک (صفر، 1 و 2 میلی­مولار) به­عنوان فاکتور اول و متیل­جاسمونات در سه سطح (صفر، 5/0 و 1 میلی­مولار) به­عنوان فاکتور دوم بودند. صفات مورد ارزیابی شامل صفات مورفولوژیکی (ارتفاع بوته، سطح برگ، تعداد گل، درصد وزن خشک بوته)، صفات فیزیولوژیکی (کلروفیل کل، کاروتنوئید، فلاونوئید) و فعالیت آنزیم­های آنتی­اکسیدانی پراکسیداز و کاتالاز و همچنین درصد اسانس بودند. بر پایه نتایج به‌دست­آمده در این پژوهش، اثر متقابل 1 میلی­مولار اسید­سالیسیلیک و 5/0 میلی­مولار متیل­جاسمونات بیشترین اثر را بر فعالیت آنزیم­های کاتالاز و پراکسیداز داشت. کاربرد برگی اسید­سالیسیلیک 2 میلی­مولار باعث افزایش ارتفاع، سطح برگ و تعداد گل شد. در بین غلظت­های مختلف متیل­جاسمونات، غلظت 1 میلی­مولار این ماده بیشترین اثر را بر مقدار کلروفیل کل، کاروتنوئید و فلاونوئید در مقایسه با تیمارهای اسیدسالیسیلیک داشت و غلظت 2 میلی­مولار اسید­سالیسیلیک، بیشترین اثر را بر مقدار درصد اسانس نسبت به تیمارهای متیل­جاسمونات بر جای گذاشت. با توجه به نتایج این پژوهش، در مجموع اثر ساده کاربرد برگی 2 میلی­مولار اسید­سالیسیلیک و 1 میلی­مولار متیل­جاسمونات توانست اکثر صفات اندازه­گیری­شده در این پژوهش را نسبت به تیمار شاهد به­طور معنی­داری بهبود بخشد.
 

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Effect of salicylic acid and methyl jasmonate on morphological traits, enzymatic activity and essential oil percentage of purple coneflower plant (Echinacea purpurea L.) in Zanjan climate

نویسندگان [English]

  • Yasin Dastyar 1
  • Mitra Aelaei 2
  • Azizollah Kheiry 2
1 Former M. Sc. Student, Faculty of Agriculture, University of Zanjan, Iran
2 Assistant Professor, Faculty of Agriculture, University of Zanjan, Iran
چکیده [English]

In order to evaluate the effects of salicylic acid, methyljasmonate and their interactions on some features of Echinacea purpurea L., a factorial experiment was conducted based on randomized complete block design with three replicates in Zanjan climate conditions. Treatments included three levels of salicylic acid (0, 1 and 2 mM) as first factor and methyl jasmonate at three levels (0, 0.5 and 1 mM) as second factor. Traits which were studied were as follow: plant height, leaf area, number of flowers and percentage of plant dry weight as morphological traits, total chlorophyll, carotenoids and flavonoids as physiological traits, activity of antioxidant enzymes such as peroxidase and catalase and essential oil percentage. Results showed that the interaction of 1 mM salicylic acid and 0.5 mM methyl jasmonate had the highest effect on the activity of catalase and peroxidase enzymes activity. Foliar application of salicylic acid at the 1 mM increased the height, leaf area and number of flowers. Among the different concentrations of methyl jasmonate, the concentration of 1 mM had the highest effect on total chlorophyll, carotenoids and flavonoids compared to salicylic acid treatments and the concentration of 2 mM salicylic acid which had the highest effect on essential oil percentage compared to methyl jasmonate treatments. In conclusion, the simple effect of 2 mM salicylic acid and 1 mM methyl jasmonate foliar application significantly improved most of the traits measured in this study compared to control treatment.

کلیدواژه‌ها [English]

  • Carotenoids
  • Chlorophyll
  • number of flowers
  • Peroxidase
  1. Abdul Jaleel, C. K., Riadh, R., Gopi, P., Manivannan, J., Ines, H. J., Al-Juburi, Z., Chang-Xing, S., Hong-Bo & Panneerselvam, R. (2009). Antioxidant defense responses: physiological plasticity in higher plants under abiotic constrains. Acta Physiologiae Plantarum, 31, 427-436.
  2. Agarwal, S., Sairam, K. R., Srivastava, Aruna, G. C. T. & Meena, C. R. (2005). Role of ABA, Salicylic acid, calcium and hydrogen peroxide on antioxidant enzyme induction in wheat seedlings. Journal Plant Sciences, 169, 559-570.
  3. Aldesuquy, H. S., Mankarios, A. T. & Awad, H. A. (1998). Effect of some antitranspirants on growth, metabolism and productivity of salinetreated wheat plants. Induction of stomatal closure, inhibition of transpiration andimprovement of leaf turgidity. Acta Botanica Hungarica, 41, 1-10.
  4. Bernath, J. (2000). Medicinal and aromatic plants. (In Hungarian). Mezo. Pub1. Budapest, pp. 556.
  5. Chance, B. & Maehly, A. C. (1955). Assay of catalases and peroxidases. Methods in Enzymology, 2, 764-775.
  6. Chen, J., Cheng, Z. & Zhong, S. (2007). Effect of exogenous salicylic acid on growth and H2O2 Metabolizing enzymes in rice seedlings lead stress. Journal of Environmental sciences, 19, 44-49.
  7. Closas, L. M., Toro, F. J., Calvó, G. & Pelacho, A. M. (1999). Effect of Methyl Jasmonate on the first developmental stages of globe artichoke. Acta Horticulturae, 660, 185-190.
  8. Comparot, S. M., Graham, C. M. & Reid, D. M. (2002). Methyl jasmonate elicits a differential antioxidant response in light and dark grown canola (Brassica napus) roots and shoots, Journal Plant Growth Regulation, 38, 21-30.
  9. Creelman, R. A. & Mullet, J. E. (1997). Biosynthesis and action of jasmonates in plants. Annual Review of Plant Physiology and Plant Molecular Biology, 48, 355-381.
  10. Creelman, R. A. & Mullet, J. E. (1995). Jasmonic acid distribution and action in plant: Regulation during development and response to biotic and abiotic stress. Proceedings of the National Academy of Sciences, U.S.A. 92, 4114-4119.
  11. Farooq, M., Wahid, A., Kobayashi, N., Fujita, D. & Basra, S. M. A. (2009). Plant drought stress effects. Mechanismsand management. Agronomy forSustainable Development, 29, 185-212. (in Farsi)
  12. Fatma, A. E. & Gharib, L. (2007). Effect of salicylic acid on the growth, metabolic activities and oil content of basil and marjoram. International Journal of Agriculture and Biology, 4, 485-492. (in Farsi)
  13. Gharib, F. A. L. (2006). Effect of salicylic acid on the growth, metabolic activities and oil content of basil and marjoram. International Journal of Agriculture & Biology, 4, 485-492.
  14. Goyal, S. & Ramawat, K. (2008). Ethrel treatment enhanced isoflavonoids accumulation in cell suspension cultures of Pueraria tuberosa, a woody legume. ActaPhysiologiaePlantarum, 30(6), 849-853.
  15. Gruenwald, J., Brendler, T. & Jaenicke, C. (1999). DDR for herbal medicines. Medical Economics Co., New Jersy, USA.Li, Th.S.C. 1998. Echinacea: Cultivation and medicinal value. Hort. Technology, 8, 122-129.
  16. Haji Reza, M. R., Hadavi, A., Zinanlou, A. A., Mirzapur, M. E. & Nayini, M. R. (2013). Effect of different levels of citric acid and salicylic acid on the pre-harvest stage on Rosa hybrida L. cutting branch. Science and Technology of Greenhouse Cultivation, 16 (4), 99-108. (in Farsi)
  17. Hamada, A. M. & Al-Hakimi, A. M. A. (2001). Salicylic acid versus salinity drought-induced stress on wheat seedlings.RostlinaVyroba, 47, 444-450. (in Farsi)
  18. Hayat, A. & Ahmad, T. (2007). Salicylic Acid A plant Hormone, Salicylic acid: biosynthesis, metabolism and physiological role in plant. Journal Scientia Horticulturae, 110, 97-98.
  19. Hayat, Q., Hayat, Sh., Irfan, M. & Ahmad, A. (2010). Effect of exogenous salicylic acid under changing environment. A review. Environmental and Experimental Botany, 68, 14-25.
  20. Jabbarzadeh, Z., Khosh-Khui, M. & Salehi, H. (2009). The effect of foliar-applied salicylic acid on flowering of African violet. Australian Journal of Basic and Applied Sciences, 3(4), 4693-4696. (in Farsi)
  21. Jung, S. (2004). Effect of chlorophyll reduction in Arabidopsis thaliana by methyl jasmonate or norflurazon on antioxidant systems. Plant Physiology and Biochemistry, 42, 225-231.
  22. Kabiri, R., Farahbakhsh, H. & Nasibi, F. (2012). Salicylic acid ameliorates the effects of oxidative stress induced by water deficit in hydroponic culture of Nigella sativa. Stress Physiology and Biochemistry, 12(11), 1420-1425.
  23. Kaijv, M., Sheng, L. & Chao, C. (2006).Antioxidation of flavonoids of green rhizome. Food Science, 27, 110-115.
  24. Kamali, M., Kharazi, S., Tehranifar, M. & Selahvarzi, Y. (2013). Effect Salicylic acid on growth and some morpho-physiological traits of Gompherna globosa L. under salt stress. Journal of Horticultural Science, 26(1), 104-112. (in Farsi)
  25. Keramat, B. & Daneshmand, F. (2012). Dual role of methyl jasmonate on physiological yields in soybean (Glycine mex L.). Process and Plant Function, 1(1), 37-26. (in Farsi)
  26. Khurana, J. P. & Cleland, C. F. (1992). Role of salicylic acid and benzoic acid in flowering of a photoperiod-insensitive strain, Lemna paucicostata LP6. Plant Physiology, 100(3), 1541-1546.
  27. Kovacik, J., Gruz, J., Backor, M., Strand, M. & Repcak, M. (2009). Salicylic acid induced changes to growth and phenolic metabolism in Matricaria chamomilla plant. Plant cell Reports, 28, 135-143.
  28. Li, N., Parson, B. L., Liu, D. R. &Mattoo, A. K. (1992). Accumulation of wound-inducible ACC syntheses transcript in tomato fruit is inhibited by salicylic acid and polyamines. Plant Molecular Biology, 18, 477-487.
  29. Li, Th. S. C. (1998). Echinacea: Cultivation and medicinal value. Hort Technology, 8(2), 122-129.
  30. Lorenzo, O. (2003). Ethylene response factor 1 integrates signals from ethylene and jasmonate pathways in plant defense. Plant Cell, 15, 165-178.
  31. Maciejewska, W. & Krupa, Z. (2002). Jasmonic acid and heavy metals in Arabidopsis plant a similar physiological response to both stressors. Plant Physiology, 159, 509-515.
  32. OmidBeigi, R. (2010). Production and processing of medicinal plants, Volume 4, Astan Quds Razavi Printing and Publishing Institute, Mashhad. (in Farsi)
  33. Pacheco, A. C., Cabral, C., Fermino, E. S. & Aleman, C. C. (2013). Salicylic acid induced changes to growth, flowering and flavonoids production in marigold plants. Global Journal of Medicinal Plant Reserch, 1(1), 95-100.
  34. Pinot, F., Benveniste, I., Salaun, J. & Durst, F. (1998). Methyl jasmonate induces lauric acid γ-hydroxylase activity and accumulation of CYP94A1 transcripts but does not affect epoxide hydrolase activities in Vicia sativa seedlings. Plant physiology, 118, 1481-1486.
  35. Popova, L., Ananieva, E., Hristova, V., Christov, K., Georgieva, K., Alexieva, V. & Stoinova, Z. H. (2003). Salicylic acid and methyl jasmonate induced protection on photosynthesis to paraquat oxidative stress. Bulgarian Journal of Plant Physiology, 18, 133-152.
  36. Qanati, F., Bakhtiyar, S. & Abdolmalaki, P. (2010). Effect of methyl jasmonate on secondary metabolites of the Marigold plant (Calendula officinalis L.). Journal of Biotechnology of TarbiatModarres University, 1 (3), 12-23. (in Farsi)
  37. Raskin, I. (1992). Role of salicylic acid in plants. Journal Annu Rev Plant MolBoil, 43, 463-739.
  38. Reymond, P. (2000). Differential gene expression in response to mechanical wounding and insect feeding in Arabidopsis. The Plant Cell, 12, 707-719.
  39. Salimi, F., Shakari, F., Azimi, M. R. & Zangani, A. (2011). The role of methyl jasmonate in improving salinity resistance through effect on some physiological characteristics of German chamomile (Matricaria chamomilla L.). Research on Medicinal and Aromatic Plants of Iran, 27, 711-700. (in Farsi)
  40. Seo, S., Ishizuka, K. & Ohash, Y. (1995). Induction of salicylic acid beta-glucosidase in tobacco (Nicotiana tabacum) leaves by exogenous salicylic acid. Plant and Cell Physiol, 36 (3), 447-453.
  41. Seyed Hajizadeh, H. & Aliloo, A. A. (2013). The effectiveness of per-harvest salicylic acid application on physiological traits in Lilium (Lilium longiflorum L.) cut flower. International Journal Science Environ, 1(12), 344-350. (in Farsi)
  42. Tayeb, M. A. (2005). Response of barley grains to the interactive effect of salinity and salicylic acid. Journal of Plant Growth Regul, 45, 215-224.
  43. Vatankhah, A., Kalantari, B. & Andalibi, B. (2016). Effect of methyl jasmonate and salinity stress on physiological and phytochemical properties of peppermint (Mentha piperita L.). Research on Medicinal and Aromatic Plants of Iran, 33, 465-449. (in Farsi)
  44. Ververidis, F., Trantas, E., Douglas, C., Vollmer, G., Kretzschmar, G. & Panopoulos, N. (2007). Biotechnology of flavonoids and other phenylpropaoid-derived natural products. Part I: Chemical diversity, impacts on plant biology and human health. Biotechnology Journal, 2(10).
  45. Vicente, M. R. & Plasencia, J. (2011). Salicylic acid beyond defence: Its role in plant growth and development. Journal of Experimental Botany, 62, 3321-3338.
  46. Walia, H., Wilson, C., Condamine, P., Liu, X., Ismail, A. & Close, T. (2007). Large-scale expression profiling and physiological characterization of jasmonic acid-mediated adaptation of barley to salinity stress. Journal of Plant, Cell & Environment, 4, 410-421.
  47. Wong, C. E., Singh, M. B. & Bhalla, P. L. (2009). Floral initiation process at the soybean shoot apical meristem may involve multiple hormonal pathways. Plant Signaling & Behavior, 7, 648-651.
  48. Zhao, X., Tan, H. J., Liu, Y. B. & Li, X. R. G. X. C. (2005). Effect of salt stress on growth and osmotic regulation in Thellungiella and Arabidopsis callus.Hen Plant Cell Tiss Organ Cult, 98, 97-103.