تأثیر اسید‌سالیسیلیک و متیل‌جاسمونات بر ویژگی‌های مورفولوژیکی، فعالیت آنزیمی‌های آنتی‌اکسیدانی و درصد اسانس گیاه سرخارگل (Echinacea purpurea L.) در شرایط اقلیمی زنجان

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی سابق کارشناسی ارشد، دانشکده کشاورزی، دانشگاه زنجان

2 استادیار، دانشکده کشاورزی، دانشگاه زنجان

چکیده

به­­منظور ارزیابی اثر اسید­سالیسیلیک و متیل­جاسمونات و اثرات متقابل آنها بر برخی از ویژگی­های گیاه سرخارگل، آزمایشی به­صورت فاکتوریل بر پایه طرح بلوک­های کامل تصادفی در سه تکرار، در شرایط اقلیمی زنجان اجرا شد. تیمارهای آزمایش شامل سه سطح اسیدسالیسیلیک (صفر، 1 و 2 میلی­مولار) به­عنوان فاکتور اول و متیل­جاسمونات در سه سطح (صفر، 5/0 و 1 میلی­مولار) به­عنوان فاکتور دوم بودند. صفات مورد ارزیابی شامل صفات مورفولوژیکی (ارتفاع بوته، سطح برگ، تعداد گل، درصد وزن خشک بوته)، صفات فیزیولوژیکی (کلروفیل کل، کاروتنوئید، فلاونوئید) و فعالیت آنزیم­های آنتی­اکسیدانی پراکسیداز و کاتالاز و همچنین درصد اسانس بودند. بر پایه نتایج به‌دست­آمده در این پژوهش، اثر متقابل 1 میلی­مولار اسید­سالیسیلیک و 5/0 میلی­مولار متیل­جاسمونات بیشترین اثر را بر فعالیت آنزیم­های کاتالاز و پراکسیداز داشت. کاربرد برگی اسید­سالیسیلیک 2 میلی­مولار باعث افزایش ارتفاع، سطح برگ و تعداد گل شد. در بین غلظت­های مختلف متیل­جاسمونات، غلظت 1 میلی­مولار این ماده بیشترین اثر را بر مقدار کلروفیل کل، کاروتنوئید و فلاونوئید در مقایسه با تیمارهای اسیدسالیسیلیک داشت و غلظت 2 میلی­مولار اسید­سالیسیلیک، بیشترین اثر را بر مقدار درصد اسانس نسبت به تیمارهای متیل­جاسمونات بر جای گذاشت. با توجه به نتایج این پژوهش، در مجموع اثر ساده کاربرد برگی 2 میلی­مولار اسید­سالیسیلیک و 1 میلی­مولار متیل­جاسمونات توانست اکثر صفات اندازه­گیری­شده در این پژوهش را نسبت به تیمار شاهد به­طور معنی­داری بهبود بخشد.
 

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Effect of salicylic acid and methyl jasmonate on morphological traits, enzymatic activity and essential oil percentage of purple coneflower plant (Echinacea purpurea L.) in Zanjan climate

نویسندگان [English]

  • Yasin Dastyar 1
  • Mitra Aelaei 2
  • Azizollah Kheiry 2
1 Former M. Sc. Student, Faculty of Agriculture, University of Zanjan, Iran
2 Assistant Professor, Faculty of Agriculture, University of Zanjan, Iran
چکیده [English]

In order to evaluate the effects of salicylic acid, methyljasmonate and their interactions on some features of Echinacea purpurea L., a factorial experiment was conducted based on randomized complete block design with three replicates in Zanjan climate conditions. Treatments included three levels of salicylic acid (0, 1 and 2 mM) as first factor and methyl jasmonate at three levels (0, 0.5 and 1 mM) as second factor. Traits which were studied were as follow: plant height, leaf area, number of flowers and percentage of plant dry weight as morphological traits, total chlorophyll, carotenoids and flavonoids as physiological traits, activity of antioxidant enzymes such as peroxidase and catalase and essential oil percentage. Results showed that the interaction of 1 mM salicylic acid and 0.5 mM methyl jasmonate had the highest effect on the activity of catalase and peroxidase enzymes activity. Foliar application of salicylic acid at the 1 mM increased the height, leaf area and number of flowers. Among the different concentrations of methyl jasmonate, the concentration of 1 mM had the highest effect on total chlorophyll, carotenoids and flavonoids compared to salicylic acid treatments and the concentration of 2 mM salicylic acid which had the highest effect on essential oil percentage compared to methyl jasmonate treatments. In conclusion, the simple effect of 2 mM salicylic acid and 1 mM methyl jasmonate foliar application significantly improved most of the traits measured in this study compared to control treatment.

کلیدواژه‌ها [English]

  • Carotenoids
  • Chlorophyll
  • number of flowers
  • Peroxidase

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علوم باغبانی ایران
دوره 50، شماره 1
خرداد 1398
صفحه 91-103

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سابقه مقاله

  • تاریخ دریافت: 05 آذر 1396
  • تاریخ بازنگری: 07 اسفند 1396
  • تاریخ پذیرش: 20 اسفند 1396

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