بررسی چندشکلی نشانگرهای مولکولی SSR و ISSR در نژادگان‌های مختلف لیموترش بدون علائم بیماری جاروک

نوع مقاله: مقاله کامل

نویسندگان

1 دانشجوی سابق دکتری، دانشکده کشاورزی، دانشگاه گیلان، رشت، ایران

2 استاد، دانشکده کشاورزی، دانشگاه گیلان، رشت، ایران

3 دانشیار، پژوهشکدۀ مرکبات و میوه‌های نیمه گرمسیری، رامسر، ایران

4 استادیار، پژوهشکدۀ مرکبات و میوه‌های نیمه گرمسیری، رامسر، ایران

5 استادیار، دانشکده کشاورزی، دانشگاه گیلان، رشت، ایران

چکیده

لایم‌ها به دلیل کیفیت و اسیدیتۀ بالای عصارۀ میوه، به‌صورت تازه خوری و فرآوری شده استفاده می‌شود. در سال‌های اخیر، رخداد بیماری جاروک، باعث بین رفتن هزاران اصله درخت لایم در جنوب ایران ‌شده است. مهم‌ترین روش کنترل این بیماری، کاشت نژادگان (ژنوتیپ)های متحمل است. بنابراین، در این پژوهش، تنوع ژنتیکی 33 نژادگان مختلف لایم بدون علائم بیماری جاروک در مزرعه یا پس از آزمایش‌های مقدماتی، به‌همراه ده رقم تجاری مرکبات به‌عنوان شاهد با 17 جفت آغازگر SSR و 11 آغازگر ISSR بررسی شد. با توجه به محتوای اطلاعات چندشکلی (PIC) نشانگرهای ریزماهواره TAA1 و TC26 به ترتیب به میزان 72/0 و 68/0 و همچنین در نشانگرهای ISSR، آغازگرهای ISSR1، 809 و 810 هر سه به میزان 49/0 به‌عنوان مناسب‌ترین نشانگرهای متمایزکننده شناسایی شدند. نتایج نشان داد، بین لایم‌ها و دیگر انواع مرکبات از لحاظ نشانگرهای مورد بررسی تفاوت‌هایی وجود دارد. از سوی دیگر همۀ نژادگان‌های لایم بر پایۀ هر دو نشانگر، قرابت بالایی با رقم‌های مکزیکن لایم و پرشین لایم نشان دادند و در یک خوشه قرار گرفتند. بنابراین، بیشتر نژادگان‌های لیموترش در جنوب کشور متعلق به گروه لایم‌ها هستند.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Investigation of polymorphism in different acid lime (Citrus aurantifolia Swingle) genotypes with free witches broom disease by molecular markers of SSR and ISSR

نویسندگان [English]

  • Samaneh Raheb 1
  • Mahmood Ghasemnezhad 2
  • Behroz Golain 3
  • Morteza Golmohammadi 4
  • Atefeh Sabori 5
1 Former Ph. D. Student, Faculty of Agriculture, University of Guilan, Rasht, Iran
2 Professor, Faculty of Agriculture, University of Guilan, Rasht, Iran
3 Associate Professor, Citrus and Subtropical Fruits Research Center, Ramsar, Iran
4 Assistant Professor, Citrus and Subtropical Fruits Research Center, Ramsar, Iran
5 Assistant Professor, Faculty of Agriculture, University of Guilan, Rasht, Iran
چکیده [English]

Limes have been consumed as fresh fruits and processing products, due to high quality and acidity in fruit juice. In recent years, the incidence of Witches Broom disease resulted in destroy of thousands Limes trees in the south of Iran. The best method for controlling this disease is planting tolerant genotypes. Therefore, in this study, genetic diversity of 33 different lime genotypes which didn’t showed Witches Broom disease in the field and preliminary test, with 10 commercial citrus cultivars as a control was evaluated by 17 pair primers of SSR and 11 primers of ISSR. According to results with Simple Sequence Repeat (SSR) markers, the highest Polymorphic Information Content (PIC) were observed in TAA1 and TC26 with 0.72 and 0.68, respectively and by using ISSR markers were in ISSR1, 809 and 810 with 0.49 which was identified as a the most appropriate distinguishing markers. Results showed that there were differences between Limes and other types of citrus based on kind of studied markers. On the other hand, all of Lime genotypes had high affinity with Mexican Lime and Persian Lime and grouped in a cluster. Therefore, most of acid limes in south of Iran belonged to Limes group.

کلیدواژه‌ها [English]

  • citrus
  • Genetic diversity
  • lime
  • polymorphic information content
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