اثر سالیسیلیک‌اسید و متیل‌جاسمونات بر تولید هایپریسین در کشت کالوس گل راعی (Hypericum perforatum L.) رقم Topas

نوع مقاله: مقاله کامل

نویسندگان

1 دانشجوی کارشناسی ارشد علوم باغبانی گرایش بیوتکنولوژی و ژنتیک مولکولی، دانشکده علوم زراعی، دانشگاه علوم کشاورزی و منابع طبیعی ساری

2 استادیار، گروه علوم باغبانی، دانشکده علوم زراعی، دانشگاه علوم کشاورزی و منابع طبیعی ساری

3 دانشیار، گروه علوم باغبانی، دانشکده علوم زراعی، دانشگاه علوم کشاورزی و منابع طبیعی ساری

چکیده

در این تحقیق اثر سالیسیلیک­اسید و متیل­جازمونات بر تولید هایپریسین در کشت کالوس گلراعی (Hypericum  perforatum L.)  رقمTopas  بررسی گردید. این آزمایش به­صورت فاکتوریل در قالب طرح کاملاً تصادفی با دو فاکتور شامل سالیسیلیک­اسید در چهار سطح (225،150،75،0 میکرومولار) و متیل­جاسمونات در سه سطح (150،75،0 میکرومولار) با سه تکرار در آزمایشگاه گروه پژوهشی علوم­باغبانی دانشگاه علوم­کشاورزی و منابع­طبیعی ساری انجام گرفت. فاکتور­های اندازه­گیری­شده شامل هایپریسین، فنل کل، فلاونوئید کل، آنتوسیانین کل و فعالیت آنزیم فنیل­آلانین­آمونیالیاز (PAL) بود. در مقایسه میانگین اثرات ساده تیمارها، اثر متیل­جازمونات بر فلاونوئید کل معنی­دار شد. اثر متقابل تیمارها برای هایپریسین، فلاونوئید­ها و آنتوسیانین­ها معنی­دار شد.در مجموع، در میان نتایج به‌دست‌آمده از این پژوهش، بیشترین میزان هایپریسین تولید­شده از اثر 150 میکرومولار سالیسیلیک­اسید و همین­طور اثر متقابل 225 میکرومولار سالیسیلیک­اسید (SA) در ترکیب با 75 میکرومولار متیل­جاسمونات (MJ) در مدت زمان 21 روز (سه هفته) به­دست آمد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Effect of Salicylic Asid and Methyle Jasmonat Elicitors on Hypericin production in stJohn’s wort (Hypericum perforatum L.) cv. Topas Callus culture

نویسندگان [English]

  • Mohammad Zamani 1
  • Hossein Moradi 2
  • Vida Chalavi 2
  • Seyyed Kamal Kazemitabar 3
1 M.Sc. Student of Horticultureal Science, Faculty of Agricultural Science, Sari Agricultural sciences and Natural Resources University, Iran
2 Assistant Professor, Faculty of Agricultural Science, Sari Agricultural Science and Natural Resources University, Iran
3 Associate Professor, Faculty of Agricultural Science, Sari Agricultural Science and Natural Resources University, Iran
چکیده [English]

Effects of salicylic acid and methyl Jasmonate on Hypericin production in callus culture of Hypericum perforatum L. cv Topas were investigated. This factorial experiment was carried out in a completely randomized design with two factors including the four levels of salicylic acid (225,150,75,0 µM) and methyl Jasmonate in three levels (150,75,0 µM) with three replications at the research lab Group of Sari University of Agricultural Sciences and natural resources, deprtment of Horticultural Science. Analysis of variance showed significant differences among treatments. Effect of treatments on total flavonoid Methyl Jasmonate effect was significant. Interaction effect was significant for Hypericin, flavonoids and anthocyanins. According to the results of this study in cell cultures of H. perforatum, low concentration (100 micromolar and lower) of both salicylic acid and methyl Jasmonate elicitors were effective for production of Hypericin. The results of this experiment showed that the highest amount of Hypericin produced from 150 μM salicylic acid (SA) and 225 μM salicylic acid in combination with 75 μM methyl Jasmonate (MJ) after 21 days.

کلیدواژه‌ها [English]

  • Elicitor
  • Naphtodianthrones
  • secondary metabolites
  • StJohn’s Wort
  1. Barnes, J., Anderson, L. A. & Phillipson, J. D. (2007). Herbal Medicin (3rd Ed). Pharmaceutical Press. London.UK, 710 pp.
  2. Coste, A., Vlase, L., Halmagyi, A., Deliu, C. & Coldea, G. (2011). Effects of plant growth regulators and elicitors on production of secondary metabolites in shoot cultures of Hypericum hirsutum and Hypericum maculatum. Plant Cell, Tissue and Organ Culture (PCTOC), 106, 279-288.
  3. Gadzovska, S., Maury, S., Delaunay, A., Spasenoski, M., Joseph, C. & Hagège, D. (2007). Jasmonic acid elicitation of Hypericum perforatum L. cell suspensions and effects on the production of phenylpropanoids and naphtodianthrones. Plant Cell, Tissue and Organ Culture (PCTOC), 89, 1-13.
  4. Gadzovska, S., Maury, S., Delaunay, A., Spasenoski, M., Hagege, D., Courtois, D. & Joseph, C. (2013). The influence of salicylic acid elicitation of shoots, callus, and cell suspension cultures on production of naphtodianthronesand phenylpropanoids in Hypericum perforatum L. Plant Cell Tissue and Organ Culture,113, 25-39.
    1. Gadzovska Simic, S., Tusevski, O., Maury, S., Delaunay, A., Joseph, C. & Hagège, D. (2014). Effects of Poly saccharide Elicitors on Secondary Metabolite Production and Antioxidant Response in Hypericum perforatum L. Shoot Cultures. Hindawi Publishing Corporatione Scientific World Journal, 1-10.
    2. Hamedani, M., Moradi, H. & Ghanbari, A. (2014). Effect of harvesting time and durability age on quality of blod orange (Citrus sinensis L.) cv Moro fruits. Journal of Iranian horticultural sciences (IJSH), 28(2), 252-259. (in Farsi)
    3. Jaimand, K., Rezaei, M., Mozafarian, V., Azadi, R., Naderihajibagherkendy, M., Moshkizadeh, M. & Golipour. (2007). determination of Hypericin content in leaf and Flower in 8 speies of Hypericum. Journal of Medicinal plants (JMP), 25, 49-55. (in Farsi)
    4. Mohammadi, M., Kazemitabar, K., Asili, J. & Kamali, H. (2014). Study of the antioxidant and antibac terial activity in methanolic, dichloromethan and hexane extracts of aerial parts of Cyperus longos. Journal of North Khorasan University of Medical Sciences,1, 161-167. (in Farsi)
    5. Morshedloo, M., Ebadi, A., Fatahimoghadam, M. & Yazdani, D. (2011). Study of parts of Essence, phenolic content and antioxidant activity in stGohns wort (Hypericum perforatum L.) Extract collecting from north of Iran. Journal of Medicinal Plant, 11(8), 218-226. (in Farsi)
    6. Mozafarian, V. (2012). Identification of medicinal and aromatic plants of Iran. Farhang e moaser Press. (in Farsi)
    7. Naik, P. M. & Al HKhairy, J. M. (2016). Abiotic and Biotic Elicitors Role in Secondary MetabolitesProduction through In Vitro Culture of Medicinal Plants, (pp. 247-277.) Intech.
    8. Namli, S., Isikalan, C., Akbas, F., Toker, Z. & Tilkat, E. (2014). Effects of UV-B radiation on total phenolic, flavonoid and hypericin contents in Hypericum retusum Aucher grown under in vitro conditions. Taylor&Francis, 28(24), 2286-2292.
    9. Omidbeigy, R. (2009). Production and Process of Medicinal Plant. (Volum 2). 5th Publishing. Behnashr Press (Astan-e-Ghods-e-Razavi). (in Farsi)
    10. Rajabi, A., Abaspoor, H. & Masood sinki, J. (2016). Effect of Methyl Jasmonate and salicylic acid Elicitors in stimulate the production of on the Hypericin in (Hypericum perforatum L.). New Cellular and Molecular Biotechnology Journal, 6(22), 41-50. (in Farsi)
    11. Rajaeian, S., Ehsanpour, A. & Toghyani, M. (2015). Changes in phenolic compound, TAL, PAL activity of Nicotiana rustica triggered by ethanolamine pretreatment under in vitro salt stress condition. Iranian Journal of Plant Biology, 7(26), 1-13. (in Farsi)
    12. Rezaei, A., Ghanati, F. & Behmanesh, M. (2011). Increased taxol production and release by methyl jasmonate, ultrasound, and dibutyl phthalate in hazelnut (Corylus avellana L.) cell culture. Journal of Plant Biology, 7, 55-72. (in Farsi)
    13. Samadi, S., Ghasemnezhad, A. & Alizadeh, M. (2014). Investigation on phenylalanine ammonialyase activity of artichoke (Cynara scolymus L.) affected by methyl jasmonate and salicylic acid in in-vitro conditions.journal of plant production, 21(4), 135-148. (in Farsi)
    14. Saunders, J. A. & Mcclure, J. W. (1974). The suitability of a quantitive spectrophotometric assay for phenyl alanine ammonia lyase activity in barely, buckwheat and pea seedlings. Journal of Plant Physiology, 54, 412-413.
    15. Walker, T., Bais, H. & Vivanco, J. (2002). Jasmonic acid induced hypericin production in cell suspension cultures of Hypericum perforatum L. (St. John’s wort). Phytochemistry, 60, 289-293.
    16. Wang, J., Qian, J., Yao, L. & Lu, Y. (2015). Enhanced production of flavonoids by methyl jasmonate elicitation in cell suspension culture of Hypericum perforatum. Bioresources and Bioprocessing, 2(5), 1-9.
    17. Yamaner, O., Erdag, B. & Gokbulut, C. (2013). Stimulation of the production of hypericins in in vitro seedlings of Hypericum adenotrichum by some biotic elicitors. Turkish journal of botany, 37, 153-159.
    18. Zarinkamar, F., Abdollahzadeh Zaviehjak, A., Sharifi, M. & Behmanesh, M. (2013). Effect of salicylic acid on flavonoids, apigenin, anthocyanin and carbohydrate in Matricaria chamomilla L. Iranian Journal of Plant Biology, 17, 67-74. (in Farsi)