بررسی تنوع ژنتیکی برخی ارقام گل داوودی (.Chrysanthemum morifolium Ramat) با استفاده از نشانگر مولکولی ریزماهواره (SSR)

نوع مقاله: مقاله کامل

نویسندگان

1 دانشجوی سابق کارشناسی ارشد، گروه علوم باغبانی، گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه لرستان، خرم آباد

2 استادیار، گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه لرستان، خرم‌آباد

3 دانشیار، گروه اصلاح نباتات و زراعت، دانشکده کشاورزی، دانشگاه لرستان، خرم آباد

4 استادیار، گروه علوم باغبانی، دانشکده کشاورزی دانشگاه ایلام، ایلام

چکیده

آگاهی از تنوع ژنتیکی، پیش­شرط اصلی و اولین گام در اصلاح گیاهان می‌باشد. در همین راستا، این پژوهش به بررسی تنوع ژنتیکی 20 رقم گل داوودی (.Chrysanthemum morifolium Ramat) با استفاده از نشانگر مولکولی SSR پرداخته است. نشانگرهای مورد استفاده، در مجموع توانستند 731 باند با اندازه 128 تا 404 جفت باز ایجاد کنند. تعداد باند در آغازگرهای مختلف، دامنه­ای از 22 تا 96 عدد باند در ارقام مورد بررسی بود. میانگین تعداد آلل مؤثر برای 12 ترکیب آغازگری، 25/1 آلل بود که بیشترین تعداد آلل مؤثر در پنج آغازگر JH09، JH52، JH72، JH30 و JH47 با تعداد 2 آلل مشاهده شد. درصد چندشکلی باندها در ارقام، بالا (100 درصد) بود. PIC در ارقام مورد بررسی، از 26/0 تا50/0 متغیر و به­ترتیب مربوط به آغازگر JH42و JH31بود. دندروگرام حاصل از تجزیه کلاستر ارقام در فاصله 61/0 ضریب تشابه، ارقام را در هفت خوشه اصلی قرار داد. تجزیه به مؤلفه­های اصلی نشان داد که شش مؤلفه اول در مجموع بیش از 60 درصد از کل تغییرات را توجیه کردند. در مجموع، شش آغازگر JH09،JH11، JH52، JH72، JH30 و JH47 می­توانند به­عنوان آغازگرهای مفید و مطلوب برای تفکیک ژنوتیپ­ها و ارقام گل داوودی معرفی شوند.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Investigation of genetic diversity of 20 cultivars of Chrysanthemum (Chrysanthemum morifolium Ramat.) using SSR molecular markers

نویسندگان [English]

  • Ferdos Darabi 1
  • Abdollah Ehteshamn-Nia 2
  • Farhad Nazarian-Firouzabadi 3
  • Zeynab Roein 4
1 Former M.Sc. Student, Department of Horticulture Science, Department of Horticulture Science, Faculty of Agriculture, Lorestan University, Khorrmabad, Iran
2 Assiatant Professor, Department of Horticulture Science, Faculty of Agriculture, Lorestan University, Khorrmabad, Iran
3 Associate Professor of Biotechnology, Department of Agronomy and Plant Breeding, Faculty of Agriculture, Lorestan University, Khoramabad, Iran
4 Assistant Professor, Department of Horticulture Science, Ilam University, Ilam, Iran
چکیده [English]

Awareness of genetic diversity is a prerequisite and the first step in plant breeding. In this regard, the present study investigates the genetic diversity of 20 cultivars of Chrysanthemum (Chrysanthemum morifolium. Ramat) using SSR molecular markers. The markers used in total were able to create 731 bands with sizes from 128 to 404 bp. The number of bands in different primers were range from 22 to 96 bands in the cultivars studied. The average number of effective alleles for 12 primers was 1.25 alleles with the highest number of effective alleles in five primers JH09, JH52, JH72, JH30 and JH47 with 2 alleles. The polymorphism of the bands in the cultivars were high (100%). The PIC in the cultivars under study were from 0.26 to 0.50, respectively, and related to the JH42 and JH31 primers. The dendrogram obtained from the cluster analysis of cultivars in a similarity coefficient of 0.61 placed the cultivars in the seven main clusters. The analysis of the main components showed that the first six components justify more than 60% of the total variation. Overall, six primers JH09, JH11, JH52, JH72, JH30 and JH47 can be introduced as useful and desirable for separation of genotypes and cultivars of Chrysanthemum.

کلیدواژه‌ها [English]

  • Chrysanthemum
  • Markers Index
  • polymorphic information content
  • SSR Molecular Markers
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