اثر همزیستی قارچ میکوریز بر برخی از ویژگی‌های مورفولوژیکی، فیزیولوژیکی و بیوشیمیایی نارنج سه برگچه‌ای (Poncirus trifoliate L.) تحت تنش شوری

نوع مقاله : مقاله پژوهشی

نویسندگان

1 استاد، دانشکده کشاورزی، دانشگاه بوعلی سینا، همدان، ایران

2 دانشجوی سابق دکتری، دانشکده کشاورزی، دانشگاه بوعلی سینا، همدان، ایران

3 استادیار، دانشکده کشاورزی، دانشگاه بوعلی سینا، همدان، ایران

چکیده

تأثیر همزیستی 3 گونه­ قارچ­ میکوریزا آربوسکولار (AMF) از جنس Glomus، در دو ترکیب دوتایی و سه­تایی بر ویژگی­های رشد، غلظت کلروفیل، تبادلات گازی، فلورسانس کلروفیل و فعالیت چند آنزیم­ آنتی­اکسیدان در نارنج سه­برگچه­ای (Poncirus trifoliate L.)، تحت تنش شوری مطالعه شد. دانهال­های 40 روزه نارنج سه­برگچه­ای، به گلدان­های حاوی G. intraradices×G. mosseae و G. hoi×G. intraradices×G. mosseae و بدون قارچ همزیست (شاهد) منتقل شدند. بعد از 175روز، دانهال­ها به­مدت 45 روز در معرض غلظت­های صفر (شاهد)، 35 و 70 میلی­مولار کلریدسدیم قرار گرفتند. در دانهال­های کلونیزه­شده تحت تنش شوری در مقایسه با دانهال­های کلونیزه­نشده تحت تنش شوری، به­ویژه با ترکیب سه­تایی، میزان کلونیزاسیون، وزن خشک شاخه و ریشه، تعداد برگ، ارتفاع، تعرق و راندمان فتوشیمیایی (حداکثر بازده کوانتومی در وضعیت سازگاری به تاریکی "Fv/Fm") ارتقاء یافت. تلقیح دانهال­ها با میکوریزا، در شرایط شوری، خصوصاً استفاده از ترکیب سه قارچ، منجر به افزایش در فعالیت آنزیم­های آنتی­اکسیدان (کاتالاز و آسکوربات­پراکسیداز) در مقایسه با شاهد گردید. به­طور کلی، AMF توانست دانهال­ها را در برابر شوری، از طریق افزایش رشد و کاهش آسیب اکسیداتیو، کمک کند.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Effect of mycorrhizal fungi symbiosis on some morphological, physiological and biochemical characteristics in trifoliate orange (Poncirus trifoliate L.) under salinity stress

نویسندگان [English]

  • Mahmood Esna-Ashari 1
  • Marzieh Hadian Deljou 2
  • Asghar Mirzaie Asl 3
1 Professor, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran
2 Former Ph. D. Student, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran
3 Assistant Professor, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran
چکیده [English]

Symbiosis effect of three arbuscular mycorrhizal fungi (AMF) species from the Glomus genus with two and three mixtures were studied on growth characteristics, chlorophyll concentration, gas exchange, chlorophyll florescence and the activity of some antioxidant enzymes in trifoliate orange (Poncirus trifoliate L.) under salinity stress. 40 days old trifoliate orange seedlings were transferred into the pots containing G. intraradices×G. mosseae, G. hoi×G. intraradices×G. mosseae and no fungi (control). After 175 days, seedlings were exposed to 0 (control), 35 and 70 mM sodium chloride for 45 days. Mycorrizal colonization, shoot and root dry weight, leaf number, plant height, transpiration rate and photochemical efficiency (maximum quantum yield through the dark adaptation “Fv/Fm”) were increased in colonized seedlings under salinity stress specially when the three mixture of fungi were used compared with the non-AMF seedlings under salinity stress. Seedling colonization particularly with three fungi mixture caused increase in the activity of antioxidant enzymes (catalase and ascorbate peroxidase) compared with the control. Overall, AMF colonization could help trifoliate orange seedlings against salt stress through increasing growth and oxidative damage reduction.

کلیدواژه‌ها [English]

  • Antioxidant Enzymes
  • Colonization
  • photosynthesis
  • photochemical efficiency
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