بهینه‌سازی القای ریشه‌های مویین در کاسنی و تأثیر اکسین و منبع کربن بر رشد آن‌ها

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی سابق کارشناسی ارشد، کشاورزی و منابع طبیعی، دانشگاه محقق اردبیلی، اردبیل، ایران

2 دانشیار، دانشکده کشاورزی و منابع طبیعی، دانشگاه محقق اردبیلی، اردبیل، ایران

3 استاد، دانشکده کشاورزی و منابع طبیعی، دانشگاه محقق اردبیلی، اردبیل، ایران

چکیده

کشت ریشه‌های مویین، روش مؤثری برای تولید متابولیت‌های ثانویه گیاهی می‌باشد؛ زیرا ریشه‌های مویین از پایداری ژنتیکی و بیولوژیکی برخوردار بوده و قادر به تولید متابولیت‌ها، در زمان کوتاه و بدون نیاز به هورمون می‌باشند. کاسنی، از جمله گیاهان دارویی ارزشمند بوده و شامل ترکیبات دارویی مهمی می‌باشد. در این تحقیق، القای ریشه‌های مویین، توسطاگروباکتریوم رایزوژنز سویه A4 انجام شد. در آزمایش اول، تأثیر نوع و سن ریزنمونه و مدت هم‌کشتی، بر کارایی القای ریشه‌های مویین بررسی شد. در آزمایش دوم، تأثیر سه نوع محیط کشت مختلف (موراشیک و اسکوگ جامد، مایع و 2/1 غلظت مایع) بر میزان رشد و تولید زیست‌توده در ریشه‌های مویین بررسی شد. در آزمایش سوم، تأثیر نوع و سن ریزنمونه، بر میزان فنل کل بررسی گردید؛ همچنین در آزمایش چهارم، تأثیر غلظت‌های مختلف نفتالین­استیک­اسید (5/1 و 1، 5/0، 0 میلی‌گرم در لیتر) و ساکارز (6 و 5، 4، 3 درصد)، بر میزان تجمع زیست‌توده بررسی شد. نتایج به­دست­آمده نشان داد که بیشترین درصد القای ریشه‌های مویین (60 درصد) و تعداد ریشه (61/11 ریشه در هر ریزنمونه) در ریزنمونه‌های کوتیلدون 5 روزه، به­دست آمد. همچنین محیط کشت 2/1 غلظت، بهترین محیط کشت برای رشد ریشه‌های مویین بود و بیشترین میزان فنل کل، در ریشه‌های مویین حاصل از برگ 28 روزه حاصل شد. همچنین محیط کشت حاوی ترکیب 5/0 میلی‌گرم در لیتر نفتالین­استیک­اسید و 3 درصد ساکارز، بهترین تأثیر را در تجمع زیست­توده نشان دادند.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Optimization of hairy roots induction in chicory (Cichorium intybus L.) and effects of auxin and carbon source on their growth

نویسندگان [English]

  • Roghayeh Fathi 1
  • Mehdi Mohebodini 2
  • Esmaeil Chamani 3
1 Former M.Sc. Student, Faculty of Agriculture and Natural resources, University of Mohaghegh Ardabili, Ardabil, Iran
2 Associate Professr, Faculty of Agriculture and Natural resources, University of Mohaghegh Ardabili, Ardabil, Iran
3 Professor, Faculty of Agriculture and Natural resources, University of Mohaghegh Ardabili, Ardabil, Iran
چکیده [English]

Hairy root cultures are an effective method for production of secondary metabolites, because hairy roots are genetically and biologically stable and they are able to produce metabolite within a short time without need to use hormones. Chicory (Cichorium intybus L.) is one of the important medicinal plants that contains a number of important medicinal compounds.In this research, hairy root induction was established through the mediation of the A4 strain of Agrobacterium rhizogenes. In first experiment, the effects of type and age of explant and co-culture times on the efficiency of hairy root induction were investigated. In the second experiment, the effect of three different media (Murashige and Skoog solid, liquid and liquid half strength) were investigated on growth rate and biomass accumulation hairy roots. In third experiment, effects of type and age of explant were investigated on the total phenolic content of hairy roots. In fourth experiment, the effect of various concentrations of NAA (0, 0.5, 1 and 1.5 mg/l) and sucrose (3, 4, 5 and 6%) were investigated on biomass accumulation. The obtained results showed maximum hairy root induction (60 percent) and number of roots (11.61 roots per explant) induced from 5-days-old cotyledons. The half strength medium of liquid Murashige and Skoog was the best medium for growth of hairy roots. Also, maximum total phenolic content in hairy roots was obtained by 28-days-old leaves. Medium supplemented with combination of 0.5 mg/l NAA and 3% sucrose showed the best effect on biomass accumulation.

کلیدواژه‌ها [English]

  • Co-culture
  • NAA
  • RolB gene
  • secondary metabolites
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