تأثیر تنش شوری بر رشد، فعالیت آنزیم‌های پاداکسنده، پراکسیداسیون لیپیدی و کارایی نظام نوریII در خیار پیوندی روی پایه‌های کدو

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری، دانشکدۀ کشاورزی، دانشگاه تبریز

2 دانشیار، دانشکدۀ کشاورزی، دانشگاه تبریز

چکیده

شوری به‌عنوان یک تنش غیرزیستی مهم در کاهش رشد و تولید گیاهان، در نظر گرفته می­شود. شناسایی سازوکار­های بیوشیمیایی و فیزیولوژیکی که در مقاومت به تنش شوری دخالت دارند، می­تواند برای انتخاب پایه­های متحمل به شوری سودمند باشد. برای این هدف آزمایشی به‌منظور بررسی تأثیر پایه (سه پایۀ کدو شینتوزا، کبالت و روت­پاور) و تنش شوری (شاهد 0، 40، 60 و 80 میلی­مولار NaCl) روی رشد، عملکرد، سطح برگ، فعالیت آنزیم­های پاداکسنده (آنتی­اکسیدان)، مقدار مالون­دی­آلدئید (MDA) و فراسنجه (پارامتر)های نورساختی (فتوسنتزی) در برگ خیار (رقم خسیب)، 35 روز پس از تنش انجام شد. فراسنجه‌های رشدی در هر سه تیمار شوری به‌طور معنی­داری در گیاهان پیوندی نسبت به گیاهان غیر پیوندی بالاتر بود. عملکرد در گیاهان پیوندی 21-14 درصد بیشتر از گیاهان غیرپیوندی بود. فعالیت آنزیم‌های کاتالاز (CAT)، آسکوربات پراکسیداز (APX)، پلی­فنل­اکسیداز (PPO) و پراکسیداز (POD) در نتیجه تنش شوری افزایش یافت، اما این افزایش در گیاهان پیوندی 2-1/0 برابر گیاهان غیرپیوندی بود. در هر سه تیمار شوری، کاهش میزان هدایت روزنه­ای در گیاهان پیوندی در مقایسه با گیاهان غیرپیوندی به‌طور معنی­داری کمتر بود، افزون بر این پراکسیداسیون لیپیدی در گیاهان پیوندی 12-7 درصد کمتر از گیاهان غیرپیوندی بود. بیشترین بازدۀ کوانتومی نظام نوری (فتوسیستم) (Fv/Fm) II در برگ­های خیار تفاوت معنی­داری بین گیاهان پیوندی و غیرپیوندی نشان داد و این مقدار در گیاهان پیوندی 6-3 درصد بیشتر از گیاهان غیرپیوندی بود. این نتایج اشاره به این دارد که افزایش فعالیت آنزیم­های پاداکسنده، نسبت Fv/Fm و هدایت روزنه­ای در گیاهان پیوندی، مرتبط با تحمل بیشتر آن­ها به تنش شوری است.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Effect of salt stress on growth, antioxidant enzymes activity, lipid peroxidation and photosystem II efficiency in cucumber grafted on cucurbit rootstock

نویسندگان [English]

  • Esmaeil Madadkhah 1
  • Sahebali Bolandnazar 2
  • Shahin Oustan 2
1 Ph.D. Candidate, Faculty of Agriculture, University of Tabriz, Iran
2 Associate Professor, Faculty of Agriculture, University of Tabriz, Iran
چکیده [English]

Salinity is considered as one of the major abiotic stress limiting growth and productivity of plants. Identification of physiological and biochemical mechanisms involved in the resistance to salinity can be useful to select salt tolerant rootstocks. For this purpose, an experiment was conducted to investigate the effects of rootstock (three cucurbit rootstock, Shintoza, Cobalt, Rootpower) and salinity stress (0, 40, 60 and 80 mM NaCl) on growth, yield, leaf area, antioxidant enzymes activity, malondialdehyde (MDA) content and Photosynthetic parameters in cucumber (cv. Khasib) leaves were determined, 35 days after salt treatments. Plant Growth parameters in all salinity treatments were significantly higher in grafted plants than non-grafted plants. Grafted plants had 14-21% higher yield than non-grafted plants. The catalase (CAT), ascorbate peroxidase (APX), polyphenol oxidase (PPO) and peroxidase (POD) activity increased as a result of salinity stress, but this increase in grafted plant was 0.1-2 times of ungrafted plant. Reductions in stomatal conductance at the three salt treatments were significantly lower in the grafted plants in comparison to ungrafted plants. Moreover, lipid peroxidation (MDA content) in grafted plants was 7-12% less than ungrafted plants by salt stress. Maximal quantum yield of PS II (Fv/Fm) of cucumber leaves showed significant difference between grafted and ungrafted plant and this amount was 3-6 percent more than ungrafted plants. Results suggested that increase in activity of antioxidant enzymes, ratio of Fv/Fm and stomatal conductance in grafted plant could be associated with their greater tolerance to salinity stress.

کلیدواژه‌ها [English]

  • Antioxidant Enzymes
  • Grafting
  • Malondialdehyde
  • photosynthetic parameters
  • Salinity stress
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