اندام‏زایی مستقیم گیاه گل ساعتی (Passiflora caerulea L.) با استفاده از ریزنمونه‏های برگ و دمبرگ

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی سابق کارشناسی ارشد، دانشگاه کشاورزی و منابع طبیعی رامین خوزستان

2 استاد، دانشگاه کشاورزی و منابع طبیعی رامین خوزستان

3 استادیار، دانشگاه کشاورزی و منابع طبیعی رامین خوزستان

چکیده

گل ساعتی (Passiflora caerulea L.) درختچه­ای بالارونده، علفی و متعلق به تیرۀ گل ساعتی (Passifloraceae) است. یکی از بخش­های مهم بیوتکنولوژی، کشت بافت است که امکان تولید گیاهانی بدون پاتوژن و در حد انبوه را فراهم می­سازد. این پژوهش به‌منظور بررسی پتانسیل دو نوع ریزنمونۀ مختلف (قطعات برگ و دمبرگ) در راستای باززایی مستقیم گیاه گل ساعتی با استفاده از غلظت­های مختلف هورمون­های 6-بنزیل آمینوپورین (BAP)، 6 فورفوریل­ آمینو پورین (KIN) و تیدیازورون (TDZ) در ترکیب با ایندول بوتریک اسید (IBA) به‌صورت طرح کامل تصادفی و در سه تکرار انجام گرفت. نتایج نشان داد، در ریزنمونۀ دمبرگ بیشترین درصد باز‏زایی (100 درصد) و بیشترین شمار شاخساره با میانگین 9/8 عدد در محیط کشت MS حاوی 1 میلی‏گرم در لیتر BAP همراه با 1/0 میلی‏گرم در لیتر IBA به دست آمد. همچنین در ریزنمونۀ برگ بیشترین درصد باز‏زایی (66/86 درصد) و بیشترین شمار شاخساره با میانگین 6/8 عدد در محیط کشت یادشده به دست آمد.‏ در آزمایش­ ریشه­زایی، بیشترین درصد ریشه‏زایی (33/93 درصد) در محیط کشت MS حاوی 5/0 میلی­گرم در لیتر IBA به دست آمد. گیاهچه­های به‌دست‌آمده از شرایط درون شیشه­ای در گلدان قرار داده شدند و برای مدت 25 تا 30 روز پیش از انتقال به خاک در شرایط اتاق کشت نگهداری شدند که بیشتر از 90‏درصد زنده‏مانی نشان دادند. این دستورالعمل به نظر می‏رسد که استعداد کافی برای ریزازدیادی این ژرم پلاسم با ارزش را داشته باشد.

کلیدواژه‌ها


عنوان مقاله [English]

Direct organogenesis of passion flower (Passiflora caerulea L.) via leaf and petiole explants

نویسندگان [English]

  • Marziyeh Jafari 1
  • Mohammad Hossein Daneshvar 2
  • Amin Lotfi-Jalalabadi 3
1 Former M. Sc. Student, Ramin University of Agriculture and Natural Resources, Khoozestan, 63417-73637, Iran
2 Professor, Ramin University of Agriculture and Natural Resources, Khoozestan, 63417-73637, Iran
3 Assistant Professor, Ramin University of Agriculture and Natural Resources, Khoozestan, 63417-73637, Iran
چکیده [English]

Passion flower (Passiflora caerulea L.) is a climbing and herbaceous shrub belonging to the family of Passifloraceae. Tissue culture is an important part of biotechnology, which can produce plants free of pathogens and provides mass production. This study aimed to evaluate the potential of two different types of explants (leaf and petiole) in order to obtain shoot multiplication of Passiflora caerulea. Various concentrations of 6-benzyl amino purine (BAP), 6-furfuryl amino purine (KN) and Thidiazuron (TDZ) in combination with indole butyric acid (IBA) were used as a completely randomized design, in three replications. Results of this experiment showed that the highest regeneration frequency of the petiole explant (100%) and the maximum number of multiple shoots (8.9) was obtained in MS medium supplemented with 1.0 mg/l BAP along with 0.1 mg/l IBA. Also in leaf explants, the highest regeneration frequency (86.66%) as well as the maximum number of multiple shoots (8.6) were obtained in the above-mentioned medium. The highest rooting frequency (93.33%) was observed on MS medium supplemented with 0.5 mg/l IBA. In vitro-raised plantlets were potted and acclimatized under culture room conditions for 25–30 days before transfer to soil conditions, where the established plants showed more than 90 % survival. The described protocol had a high potential for the micropropagation of this valuable germplasm.

کلیدواژه‌ها [English]

  • Acclimatization
  • Regeneration
  • rooting
  • Tissue culture
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