کاهش آسیب سرمازدگی و بهبود ویژگی‌های پاداکسندگی میوۀ انار با تیمارهای پیش و پس از برداشت با کاربرد متیل‌جاسمونات

نوع مقاله : مقاله پژوهشی

نویسندگان

1 استاد، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج، ایران

2 دانشجوی سابق دکتری، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج، ایران

3 دانشیار، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج، ایران

چکیده

انار از جمله میوه‌های حساس به سرمازدگی است و نگهداری آن در دمای پایین‌تر از 5 درجۀ سلسیوس منجر به ظهور نشانه‌های سرمازدگی می‌شود. این پژوهش به‌صورت یک آزمایش ترکیبی از تیمار پیش و پس از برداشت غلظت‌های مختلف متیل‌جاسمونات (0 (شاهد)، 1/0، 2/0 و 3/0 میلی‌مولار) بر کاهش سرمازدگی و ترکیب‌های پاداکسندگی (آنتی‌اکسیدانی) و ارزش تغذیه‌ای میوۀ انار رقم ملس ساوه انجام شد. میوه‌ها در دمای 1±4 درجۀ سلسیوس و رطوبت نسبی 95-90 درصد به مدت 4 ماه نگهداری شدند. تیمار 2/0 میلی‌مولار متیل‌جاسمونات به‌طور معنی‌داری سبب کاهش شاخص سرمازدگی، میزان نشت یونی و مالون‌دی‌آلدئید به ترتیب به میزان 54، 20 و 27 درصد شد. میوه‌های تیمارشده با سطح 2/0 میلی‌مولار متیل‌جاسمونات دارای ترکیب‌های فنلی، آنتوسیانین‌های کل، ظرفیت پاداکسندگی و فعالیت آنزیم فنیل‌آلانین ‌آمونیالیاز بیشتری در مقایسه با شاهد داشتند. همچنین میوه‌های انار تیمارشده با متیل‌جاسمونات درصد کاهش وزن، مواد جامد محلول کل و pH کمتر، ویتامین ث و اسید کل بیشتری در مقایسه با میوه‌های شاهد داشتند. به‌طورکلی متیل‌جاسمونات را می‌توان به‌عنوان یک روش سودمند و کاربردی برای افزایش مقاومت میوه‌های انار به آسیب سرمازدگی و بازدارندۀ کاهش ارزش تغذیه‌ای میوه‌ها در مدت انبارمانی و انتقال آن‌ها به بازار، به‌کار برد.

کلیدواژه‌ها


عنوان مقاله [English]

Amelioration of chilling injury and improvement of antioxidants in pomegranate by pre and postharvest methyljasmonate application

نویسندگان [English]

  • Mesbah Babalar 1
  • Farhad Pirzad 2
  • Mohammad Ali Askari Sarcheshmeh 3
  • Alireza Talaei 1
  • Hossein Lessani 1
1 Professor, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
2 Former Ph.D. Student, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
3 Associate Professor, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
چکیده [English]

Pomegranate fruit is one of the sensetive fruit to chilling injury (CI), when they are exposed to temperatures below 5 °C. This study applied a combination of pre and postharvest methyl jasmonate (MeJA) at 0 (control), 0.1, 0.2 and 0.3 mM on chilling injury (CI), antioxidant capacity and fruit quality in pomegranate fruit. Fruits were stored at 4±1 °C and 90-95% relative humidity for 4 months. MeJA treatment at 0.2 mM significantly delayed the postharvest CI and increased electrolyte leakage and malondialdehyde (MDA) accumulation about 54, 20 and 27 percent, repectively. In these fruits, total phenolics, DPPH scavenging activity, Phenylalanine ammonia-lyase and total anthocyanine were found at higher levels in treated than control pomegranates during postharvest storage. Also, treated fruits had lower weight loss, total soluble solids (TSS), pH and higher TA and Vit C. Overall, these findings demonstrated that pre and postharvest treatments with MeJA had potential effect on enhancing tolerance of pomegranates to postharvest chilling injury, maintaining the quality and extending storability and marketing of pomegranate fruits.

کلیدواژه‌ها [English]

  • Anthocyanins
  • electrolyte leakage
  • phenylalanine ammonia-lyase
  • storage
  1. Asghari, M. & Hasanlooe, A. R. (2015). Methyl jasmonate effectively enhanced some defense enzymes activity and Total antioxidant content in harvested “Sabrosa” strawberry fruit. Food Science and Nutrition, 26, 377-383.
  2. Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle-dye binding. Analytical Biochemistry, 72, 248–254.
  3. Cao, S., Zheng, Y., Wang, K., Rui, H. & Tang, S. (2010). Effect of methyl jasmonate on cell wall modification of loquat fruit in relation to chilling injury after harvest. Food Chemistry, 118, 641–647.
  4. Chanjirakul, K., Wang, S. Y., Wang, C. H. & Siriphanich, J. (2006). Effect of natural volatilecompounds on antioxidant capacity and antioxidant enzymes in raspberries. Postharvest Biology and. Technology, 40, 106-115.
  5. Chanjirakul, K., Wang, S. Y., Wang, C. H. & Siriphanich, J. (2007). Natural volatiletreatments increase free-radical scavenging capacity of strawberries and black-berries. Journal of the Science of Food and Agriculture, 87, 1463-1472.
  6. Chen, J. Y., He, L. H., Jiang, Y. M., Wang, Y., Joyce, D. C., Ji, Z. L. & Lu, W. J. (2008). Role of phenylalanine ammonia-lyase in heat pretreatment-induced chilling tolerance in banana fruit. Physiologia Planartarum, 132, 318-328.
  7. Eberhardt, M. V., Lee, C. Y. & Liu, R. H. (2000). Nutrition: Antioxidant activity of fresh apples. Nature, 405, 903-904.
  8. Fan, L., Shi, J., Zuo, J., Gao, L., Lv, J. & Wang, Q. (2016a). Methyl jasmonate delays postharvest ripening and senescence in the non-climacteric eggplant (Solanum melongena L.) fruit. Postharvest Biology and Technology, 120, 76-83.
  9. Fan, L., Wang, Q., Lv, J., Gao, L., Zuo, J. & Shi, J. (2016b). Amelioration of postharvest chilling injury in cowpea (Vigna sinensis) by methyl jasmonate (MeJA) treatments. Scientia Horticulturae, 203, 95-101.
  10. Flores, G. & Del Castillo, M. L. R. (2014). Influence of preharvest and postharvest methyl jasmonate treatments on flavonoid content and metabolomic enzymes in red raspberry. Postharvest Biology and Technology, 97, 77-82.
  11. Giusti, M. M. & Wrolstad, R. E. (2001). Characterization and measurement of anthocyanins by UV-Visible spectroscopy. In: Current Protocols in Food Analytical Chemistry, 1-13.
  12. Hassanpour, H., Hamidoghli, Y., Hajilo, J. & Adlipour, M. (2011). Antioxidant capacity and phytochemical properties of cornelian cherry (Cornus mas L.) genotypes in Iran. Scientia Horticulturae, 129, 459-463.
  13. Kashash, Y., Mayuoni-Kirshenbaum, L., Goldenberg, L., Choi, H. J. & Porat, R. (2016). Effects of harvest date and low-temperature conditioning on chilling tolerance of ‘Wonderful’pomegranate fruit. Scientia Horticulturae, 209, 286-292.
  14. Kim, H. J., Fonseca, J. M., Choi, J. H. & Kubota, C. (2007). Effect of methyl jasmonate on phenolic compounds and carotenoids of romaine lettuce (Lactuca sativa L.). Journal of Agricultural and Food Chemistry, 55, 10366-10372.
  15. Knee, M. (2002). Fruit quality and its biological basis. Published by Sheffield Academic Press.
  16. Kucuker, E., Ozturk, B., Celik, S. M. & Aksit, H. (2014). Pre-harvest spray application of methyl jasmonate plays an important role in fruit ripening, fruit quality and bioactive compounds of Japanese plums. Scientia Horticulturae, 176, 162-169.
  17. Li, Z., Hao, Y., Yang, Y. & Deng, W. (2010). Molecular cloning and expression analysis of a cytochrome P450 gene in tomato. Plant growth regulation, 61, 297-304.
  18. Martinez-Espla, A., Zapata, P. J., Castillo, S., Guillén, F., Martínez-Romero, D., Valero, D. & Serrano, M. (2014). Preharvest application of methyl jasmonate (MeJA) in two plum cultivars. 1. Improvement of fruit growth and quality attributes at harvest. Postharvest Biology and Technology, 98, 98-105.
  19. Meng, X., Han, J., Wang, Q. & Tian, S. (2009). Changes in physiology and quality of peach fruits treated by methyl jasmonate under low temperature stress. Food Chemistry, 114, 1028-1035.
  20. Mirdehghan, S. H., Rahemi, M., Martinez-Romero, D., Guillén, F., Valverde, J. M., Zapata, P. J., et al. (2007b). Reduction of pomegran­ate chilling injury during storage after heat treatment: role of polyamines. Postharvest Biology and Technology, 44, 19-25.
  21. Mirdehghan, S. H., Rahemi, M., Castillo, S., Martinez-Romero, D., Serrano, M. & Valero, D. (2007a). Pre-storage application of polyamines by pressure or immersion improves shelf life of pomegranate stored at chilling temperature by increasing endogenous polyamine levels. Postharvest Biology and Technology,44, 26-33.
  22. Moreno, F. D., Monagas, M., Blanch, G. P., Bartolome, B. & Castillo, M. L. R. (2010). Enhancement of anthocyanins and selected aroma compounds in strawberryfruits through methyl jasmonate vapor treatment. European Food Research and Technology, 230, 989-999.
  23. Nanda, S., Rao, D. V. S. & Krishnamurthy, S. (2001). Effects of shrink film wrapping and storage temperature on the shelf life and quality of pomegranate fruits cv. Ganesh. Postharvest Biology and Technology, 22, 61-69.
  24. Nguyen, T. B. T., Ketsa, S. & van Doorn, W. G. (2003). Relationship between browning and the activities of polyphenol oxidase and phenylalanine ammonia lyase in banana peel during low temperature storage. Postharvest Biology and Technology, 30, 187-193.
  25. Opara, U. L., Atukuri, J. & Fawole, O. A. (2015). Application of physical and chemical postharvest treatments to enhance storage and shelf life of pomegranate fruit-A review. Scientia Horticulturae, 197, 41-49.
  26. Rahemi, M. (2008). Postharvest (An Introduction to the physiology and handling of fruit, vegetables and ornamentals. Translation. (in Farsi)
  27. Reyes-Diaz, M., Lobos, T., Cardemil, L., Nunes-Nesi, A., Retamales, J., Jaakola, L. & Ribera-Fonseca, A. (2016). Methyl jasmonate: an alternative for improving the quality and health properties of fresh fruits. Molecules, 21, 567.
  28. Saavedra, G. M., Figueroa, N. E., Poblete, L. A., Cherian, S. & Figueroa, C. R. (2016). Effects of preharvest applications of methyl jasmonate and chitosan on postharvest decay, quality and chemical attributes of Fragaria chiloensis fruit. Food chemistry, 190, 448-453.
  29. Sayyari, M. (2009). Effects of Chemical and non-chemical on resistance to chilling injury and husk scald of pomegranate in storage. Ph.D. Thesis. Faculty of Horticulturae. University of Tehran. Iran. (in Farsi)
  30. Sayyari, M., Babalar, M., Kalantari, S., Martinez-Romero, D., Guillen, F., Serrano, M. & Valero, D. (2011). Vapour treatments with methyl salicylate or methyl jasmonate alleviated chilling injury and enhanced antioxidant potential during postharvest storage of pomegranates. Food Chemistry, 124, 964-970.
  31. Sayyari, M., Babalar, M., Kalantari, S., Serrano, M. & Valero, D. (2009). Effect of salicylic acid treatment on reducing chilling injury in stored pomegranates. Postharvest Biology and Technology, 53, 152-154.
  32. Sayyari, M., Valero, D., Babalar, M., Kalantari, S., Zapata, P. J. & Serrano, M. (2010). Prestorage oxalic acid treatment maintained visual quality, bioactive compounds, and antioxidant potential of pomegranate after long-term storage at 2°C. Journal of Agricultural and Food Chemistry, 58, 6804-6808.
  33. Serrano, M., Guillen, F., Martinez-Romero, D., Castillo, S. & Valero, D. (2005). Chemical constituents and antioxidant activity of sweet cherry at different ripening stage. Journal of Agricultural and Food Chemistry, 53, 2741-2745.
  34. Siboza, X. I., Bertling, I. & Odindo, A. O. (2014). Salicylic acid and methyl jasmonate improve chilling tolerance in cold-stored lemon fruit (Citrus limon). Journal of Plant Physiology, 171, 1722-1731.
  35. Soleimani Aghdam, M., Naderi, R., Askari Sarcheshmeh, N. A. & Babalar, M. (2016). Amelioration of postharvest chilling injury in anthurium cut flowers by g-aminobutyric acid (GABA) treatments. Postharvest Biology and Technology, 110, 70-76.
  36. Valero, D., Mirdehghan, S. H., Sayyari, M. & Serrano, M. (2015). Vapor treatments, chilling, storage, and antioxidants in pomegranates. Processing and Impact on Active Components in Food, ed. by Preedy V. Academic Press, San Diego, CA, 189-196.
  37. Wang, L. J. & Li, S. H. (2006). Salicylic acid-induced heat or cold tolerance in relation to Ca2+ homeostasis and antioxidant systems in young grape plants. Plant Science, 170, 685-694.
  38. Wang, S. Y., Bowman, L. & Ding, M. (2008). Methyl jasmonate enhances antioxidant activity and flavonoid content in blackberries (Rubus sp.) and promotes antiproliferation of human cancer cells. Food Chemistry, 107, 1261-1269.
  39. Yu, L., Liu, H., Shao, X., Yu, F., Wei, Y., Ni, Z., Xu, F. & Wang, H. (2016). Effects of hot air and methyl jasmonate treatment on the metabolism of soluble sugars in peach fruit during cold storage. Postharvest Biology and Technology, 113, 8-16.
  40. Zhang, X., Sheng, J., Li, F., Meng, D. & Shen, L. (2012). Methyl jasmonate alters arginine catabolism and improves postharvest chilling tolerance in cherry tomato fruit. Postharvest biology and technology, 64, 160-167.
  41. Zhao, S. J., Xu, C. C. & Zou, Q. (1994). Improvements of the method for measurement of malondialdehyde in plant tissue. Plant Physiology Communications, 30, 207-210.
  42. Ziosi, V., Bregoli, A. M., Fregola, F., Costa, G. & Torrigiani, P. (2009). Jasmonate-induced ripening delay is associated with up-regulation of polyamine levels in peach fruit. Journal of Plant Physiology, 166, 938-946.