نوع مقاله : مقاله پژوهشی
نویسندگان
1 استادیار، دانشکده کشاورزی، دانشگاه زنجان
2 دانشجوی سابق کارشناسی ارشد، دانشکده کشاورزی، دانشگاه زنجان
3 استادیار گروه بیوتکنولوژی، پژوهشکدۀ فناوری‌های نوین زیستی، دانشگاه زنجان
چکیده
ارائۀ یک دستورکار افزونش انبوه درون شیشه‌ای برای توسعه و افزونش گل‌های زینتی ارزشمند همچون آنتوریوم یکی از مهم‌ترین فرایندها و هدف‌های اصلی در صنعت گل‌کاری است. در همین زمینه با هدف ارائۀ دستورکار تجاری افزونش انبوه درون شیشه‌ای آنتوریوم، تأثیر بنزیل آدنین (BA)1 و نفتالین استیک اسید (NAA)2 بر قابلیت باززایی و پرآوری آنتوریوم و قابلیت افزونش درون شیشه‌ای و همچنین ویژگی‌های کمی و کیفی آنتوریوم رقم صورتی با اعمال تیمارهای مختلف بررسی شد. ریزنمونههای جوانۀ جانبی در محیط کشت 2/MS حاوی غلظت‌های متفاوتی از بنزیل آدنین (3/0، 1/0، 0 میلیگرم بر لیتر) و نفتالیناستیک اسید (23/0، 07/0، 04/0،0 میلیگرم بر لیتر) کشت شد. بیشترین طول شاخساره (با میانگین 46/23 میلی‌متر) در محیط کشت حاوی 23/0 میلیگرم بر لیتر NAA به دست آمد. بیشترین شمار جوانه در محیط کشت حاوی 23/0 میلیگرم بر لیترNAA به همراه 3/0 میلیگرم بر لیترBA تولید شد. در مورد صفت شمار ریشه بیشترین میزان مربوط به تیمار 23/0 میلیگرم بر لیتر NAA بود. بیشترین درصد باززایی در تیمار 23/0 میلیگرم بر لیتر NAA + 3/0 میلیگرم بر لیتر BA و کمترین درصد باززایی در تیمار شاهد مشاهده شد. ترکیب‌بندی (فرمولاسیون) سادۀ به‌دست‌آمده می‌تواند به‌آسانی برای افزونش انبوه گیاهان سالم و بدون بیماری آنتوریوم به‌کار رود.
کلیدواژه‌ها
موضوعات
عنوان مقاله [English]
Regeneration, proliferation and commercialized micro propagation of Anthurium andraeanum cv. Pink
نویسندگان [English]
- Mitra Aelaei 1
- Hajar Narimani 2
- Abbas Bahari 3
- Jafar Mohammadi 1
1 Assist Professor, Faculty of Agriculture, University of Zanjan, Iran
2 Former M.Sc. Student, Faculty of Agriculture, University of Zanjan, Iran
3 Assistant Professor, Institute of Biological Technologies, University of Zanjan, Iran
چکیده [English]
A standard in vitro mass propagation protocol for development and propagation of valuable flowers such as Anthurium is one of the major procedures and goals in flower industry. Towards developing a commercialized propagation recipe for A. andraeanum cv. Pink, effects of BA and NAA were investigated on regeneration, proliferation and in vitro mass propagation efficiency of cultivar Pink, as well as quantitative and qualitative characteristics. The lateral buds were established in 1/2MS contains different concentrations of BA (0, 0.1, 0.3 mg/l) and NAA (0, 0.04, 0.07, 0.23 mg/l). The longest shoots (average of 23.46 mm), was obtained in 1/2MS containing 0.23 mg/l NAA .The highest number of buds were produced in medium containing 0.23 NAA plus 0.3mg/l BA. The highest root number was emerged in 0.23 mg/l NAA. The highest percentage of regeneration rate obtained in the media containing 0.23mg/l NAA+ 0.3mg/l BA compared to the control. Obtained protocol can be simply used for in vitro mass propagation of healthy Anthurium plants.
کلیدواژه‌ها [English]
- Benzyl adenine
- In vitro culture
- mass propagation
- naphthalene acetic acid
- proliferation
- Ajdarbin, M., Kafi, M., Mirmasoumi, M. & Azadi, P. (2015). Indirect Shoot Regeneration in Anthurium andreanum 'Clisto' from Leaf Explant. Journal of Ornamental Plants, 5(3), 159-166.
- Atak, Ç. & Çelik, Ö. (2012). Micropropagation of Anthurium spp. pp. 241-254. In: Dhal, N.K. and S.C. Sahu (eds.). Plant science. In Tech Press, Rijeka, Croatia. Doi: 10.5772/51426.
- Atta-Alla, H. M. C., Alister, B. G. & Van Staden, J. (1998). In vitro culture and establishment of Anthurium parvispathum South African journal of Botany, 64, 296-298.
- Bakhshi Khaniki, Gh. & Ghasemi, M. (2011). Micropropagation of Anthurium study through tissue culture. Cell-Molecular Biotechnology News, (4). (in Farsi)
- Bairamizadeh, E. & Azadi, P. (2007). The effect of growth regulators on shoot formation in Anthurium Andreanum. Research and Construction, (76), 63-70. (in Farsi)
- Bairamizadeh, E., Azadi, P. & Mii, M. (2008) Optimization of factors affecting organogenesis and somatic embryogenesis of Anthurium andreanum Lind. ‘TERA’. Propagation of Ornamental Plants, 8(4), 198-203.
- Callotte, V. (2004). Anthurium aristocracy. New Zealand Garden Journal, 7(1), 3-5.
- Cen, Y. Q., Jiang, R. M., Deng, Z. L. & Ni, D. X. (1993).In vitro propagation of Anthurium andreanum. Morphogenesis and effects of physical and chemical factors. Acta Horticulturae Sinica, 20, 187-192. (in Chinese with English abstract).
- Dufour, L. & Guerin, V. (2008). Growth developmental feature and flower production of Anthurium andreanum Lind. in tropical condition. Scientia Horticulturae, 98, 25-35.
- Gantait, S. & Sinniah, U. R. (2011). Morphology, flow cytometry and molecular assessment of ex-vitro grown micropropagated anthurium in comparison with seed germinated plants. African Journal of Biotechnology, 10(64), 13991-13998.
- Elibox, W. & Umaharan, P. (2010). Cultivar differences in the deterioration of vase-life in cut-flowers of Anthurium andraeanum is determined by mechanisms that regulate water uptake. Scientia Horticulturae, 124(1), 102-108.
- Gantait, S. & Sinniah, U. R. (2011). Grown micropropagated anthurium in comparison with seed germinated plants. Journal of Biotechnology, 10(64), 13991-13998.
- Geier, T. (1982). Morphogenesis and plant regeneration from spadix frag-ments of Anthurium scherzerianum cultivated in vitro. 5th Int. Cong. Plant Tiss. Cell Culture, Tokyo. pp. 137-138.
- Geier, T. (1982). Morphogenesis and plant regeneration from spadix fragments of Anthurium scherzerianum cultivated in vitro. In Plant tissue culture 1982: proceedings, 5th International Congress of Plant Tissue and Cell Culture held at Tokyo and Lake Yamanake, Japan, July 11-16, 1982/edited by Akio Fujiwara. Tokyo: Japanese Association for Plant Tissue Culture, [1982?].
- Gomes, F., Simoes, M., Lopes, M. L. & Canhoto, J. M. (2010). Effect of plant growth regulators and genotype on the micropropagation of adult trees of Arbutus unedo L. (strawberry tree). New Biotechnology, 27(6), 882-892.
- Hartmann, H. T., Kester, D. E., Davies, F. T. & Geneve, R. L. (1997). Plant propagation: principles and practices (No. Ed. 6). Prentice-Hall Inc.
- Hamidah, M., Debergh, P. C. & Abdul Karim, A. G. (1995). Somatic embryogenesis of Anthurium scherzerianum Schott. Mededelingen-Faculteit Landbouwkundige en Toegepaste Biologische Wetenschappen Universiteit Gent (Belgium), 60(4a), 1671-1673.
- Hedayat, M. & Khoshkhoy, M. (2005). Effect of culture media and growth regulators on micropropagation of Pyrethrum. Journal of Horticultural Science and Technology, (6), 171-182. (in Farsi)
- Ho, W. & Vasil, I. K. (1983). Somatic embryogenesis in sugarcane (Sancharrum officinarum L.). I. The morphology and ontogeny of somatic embryos. Protoplasma, 118, 169-180.
- Hua, X. (2014). Flower market research report in Chinese New Year in 2013. China Flowers Hortic, 13, 24-28.
- Jahan, M. T., Islam, M. R., Khan, R., Mamun, A. N. K., Ahmed, G. & Hakim, H. (2009). In vitro clonal propagation of Anthurium (Anthurium andraeanum) using callus culture. Plant Tissue Culture and Biotechnology, 9(1), 61-69.
- Jain, S. M. & Ochatt, S. J. (2010). Protocols for In Vitro Propagation of Ornamental Plants. Springer Protocols, Humana Press. New York.
- Kapchina, V. & Yakimova, E. (1997). Effect of puring and phenylurea cytokinins on peroxidase activity in relation to apical dominance of in vitro cultivated Rosa hybrid L. Bulgarian Journal of Plant Physiology, 23, 40-48.
- Khalighy, A. (2007). Check micropropagation and somatic emberyogenesis in the Alstromeria flower cultivar Fuego. M. Sc.thesis.Univ.Tehran. 49-58.
- Khorrami Raad, M. & Shoor, M. (2011). The effect of growth regulators on the micropropagation two commercial cultivars Anthurium andraeanum in vitro. Journal of Horticultural Science, 25(2), 137-146. (in Farsi)
- Khorrami Raad, M., Bohluli Zanjani, S., Ramezani Sayyad, A., Maghsudi, M. & Kaviani, B. (2012). Effect of Cultivar, Type and Age of Explants, Light Conditions and Plant Growth Regulators on Callus Formation of Anthurium. American-Eurasian Journal of Agricultural & Environmental Science, 12(6), 706-712.
- Kuehnle, A. R. & Sugii, N. (1991). Callus induction and plantlet regeneration in tissue cultures of Hawaiian Anthuriums. HortScience, 26(7), 919-921.
- Kunisaki, J. T. (1980). In vitro propagation of Anthurium andreanum. HortScience, 15, 508-509.
- Lee, C. W. & Thomas, J. C. (1985). Tissue culture propagation of buffalo gourd. HortScience, 20, 218-219.
- Martin, K. P., Joseph, D., Madassery, J. & Philip, V. J. (2003). Direct shoot regeneration from lamina explants of two commercial cut flower cultivars of Anthurium andraeanum Hort. In Vitro Cellular and Developmental Biology-Plant, 39(5), 500-504.
- Moradi, B. P. & Vaez livari, V. (2004). Proliferation and production of Anthurium andreanum through tissue culture system. The First National Seminar of Cut Flowers. (in Farsi)
- Nhut, D. T., Duy, N., Vy, N. N. H., Khue, C. D., Khiem, D. V. & Vinh, D. N. (2006). Impact of Anthurium spp. genotype on callus induction derived from leaf explants, and shoot and root regeneration capacity from callus. Journal of Applied Horticulture, 8(2), 135-137.
- Norton, M. E. & Boe, A. A. (1982). In vitropropagation of ornamental rosa ceous plants. HortScience, 17, 190-191.
- Rikken, M. (2010). The European Market for Fair and Sustainable Flowers and Plants. Trade for Development Centre, Belgian Development Agency, Belgium, pp. 63.
- Sedaghati, B. & Babaian Jelodar, N. (2015). Effect of leaf Explants types and various levels of 2,4-D on callus induction and plant regeneration in Anthurium andraeanum. Journal of Crop Breeding, (16). (in Farsi)
- Xilin, H. (1992). Effect of different cultivars and hormonal conditions on strawberry anther culture in vitro. Journal of Nanjing Agriculture University, 15, 21-28.
- Zeng, S. J., Yu, Z. M. & Ke, X. Y. (2004). Foliage Plant of Araceae. (pp.48-52.) China Forestry PublishingHouse.
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