باززایی، پرآوری و افزونش تجاری آنتوریوم آندرانوم رقم صورتی (Anthurium andraeanum cv Pink) به‌روش ریزازدیادی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 استادیار، دانشکده کشاورزی، دانشگاه زنجان

2 دانشجوی سابق کارشناسی ارشد، دانشکده کشاورزی، دانشگاه زنجان

3 استادیار گروه بیوتکنولوژی، پژوهشکدۀ فناوری‌های نوین زیستی، دانشگاه زنجان

چکیده

ارائۀ یک دستورکار افزونش انبوه درون شیشه‌ای برای توسعه و افزونش گل‌های زینتی ارزشمند همچون آنتوریوم یکی از مهم‌ترین فرایندها و هدف‌های اصلی در صنعت گل‌کاری است. در همین زمینه با هدف ارائۀ دستورکار تجاری افزونش انبوه درون شیشه‌ای آنتوریوم، تأثیر بنزیل آدنین (BA)1 و نفتالین استیک اسید (NAA)2 بر قابلیت باززایی و پرآوری آنتوریوم و قابلیت افزونش درون شیشه‌ای و همچنین ویژگی‌های کمی و کیفی آنتوریوم رقم صورتی با اعمال تیمارهای مختلف بررسی شد. ریزنمونه­های جوانۀ جانبی در محیط کشت 2/MS حاوی غلظت‌های متفاوتی از بنزیل آدنین (3/0، 1/0، 0 میلی‌گرم بر لیتر) و نفتالین­استیک اسید (23/0، 07/0، 04/0،0 میلی‌گرم بر لیتر) کشت شد. بیشترین طول شاخساره (با میانگین 46/23 میلی‌متر) در محیط کشت حاوی 23/0 میلی­گرم ­بر لیتر NAA به دست آمد. بیشترین شمار جوانه در محیط کشت حاوی 23/0 میلی­گرم بر لیترNAA  به­ همراه 3/0 میلی­گرم­ بر لیترBA تولید شد. در مورد صفت شمار ریشه بیشترین میزان مربوط به تیمار 23/0 میلی‌گرم بر لیتر NAA بود. بیشترین درصد باززایی در تیمار 23/0 میلی‌گرم بر لیتر NAA + 3/0 میلی‌گرم بر لیتر BA و کمترین درصد باززایی در تیمار شاهد مشاهده شد. ترکیب‌بندی (فرمولاسیون) سادۀ به‌دست‌آمده می‌تواند به‌آسانی برای افزونش انبوه گیاهان سالم و بدون بیماری آنتوریوم به‌کار رود.  

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Regeneration, proliferation and commercialized micro propagation of Anthurium andraeanum cv. Pink

نویسندگان [English]

  • Mitra Aelaei 1
  • Hajar Narimani 2
  • Abbas Bahari 3
  • Jafar Mohammadi 1
1 Assist Professor, Faculty of Agriculture, University of Zanjan, Iran
2 Former M.Sc. Student, Faculty of Agriculture, University of Zanjan, Iran
3 Assistant Professor, Institute of Biological Technologies, University of Zanjan, Iran
چکیده [English]

A standard in vitro mass propagation protocol for development and propagation of valuable flowers such as Anthurium is one of the major procedures and goals in flower industry. Towards developing a commercialized propagation recipe for A. andraeanum cv. Pink, effects of BA and NAA were investigated on regeneration, proliferation and in vitro mass propagation efficiency of cultivar Pink, as well as quantitative and qualitative characteristics. The lateral buds were established in 1/2MS contains different concentrations of BA (0, 0.1, 0.3 mg/l) and NAA (0, 0.04, 0.07, 0.23 mg/l). The longest shoots (average of 23.46 mm), was obtained in 1/2MS containing 0.23 mg/l NAA .The highest number of buds were produced in medium containing 0.23 NAA plus 0.3mg/l BA. The highest root number was emerged in 0.23 mg/l NAA. The highest percentage of regeneration rate obtained in the media containing 0.23mg/l NAA+ 0.3mg/l BA compared to the control. Obtained protocol can be simply used for in vitro mass propagation of healthy Anthurium plants.  

کلیدواژه‌ها [English]

  • Benzyl adenine
  • In vitro culture
  • mass propagation
  • naphthalene acetic acid
  • proliferation
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