بررسی تأثیر تغذیۀ برگی سیلیسیم بر شاخص‌های رشدی، فیزیولوژی و بیوشیمیایی گیاه نعناع در شرایط تنش کادمیوم

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی سابق کارشناسی ارشد، دانشکده کشاورزی، دانشگاه محقق اردبیلی

2 دانشیار، دانشکده کشاورزی، دانشگاه محقق اردبیلی

3 دانشجوی دکتری، دانشکده کشاورزی، دانشگاه محقق اردبیلی

چکیده

سیلسیم دومین عنصر فراوان در کره زمین است که وجود یا کاربرد آن تأثیر زیانبار فلزهای سنگین را بهبود می‌بخشد. به‌منظور بررسی تأثیر سیلسیم در افزایش تحمل به آلودگی کادمیوم در نعناع، آزمایشی در سال 94-1393 به‌صورت فاکتوریل در قالب طرح کامل تصادفی در چهار تکرار در گلخانۀ تحقیقاتی دانشکدۀ کشاورزی، دانشگاه محقق اردبیلی اجرا شد. تیمارهای این آزمایش شامل سطوح مختلف کادمیوم (0، 50، 100، 250 میلی­گرم در کیلوگرم) و محلول­پاشی متا سیلیکات سدیم در سطوح 0 و 1 میلی­مولار بود. شمار گلدان­ها در این آزمایش 32 عدد بود که در هر گلدان یک بوته قرار داشت. صفاتی مانند ارتفاع بوته، وزن تر اندام‌های هوایی، وزن خشک اندام‌های هوایی، وزن خشک ساقه، شمار برگ، سطح برگ، وزن خشک ریشه، محتوای نسبی آب، رنگیزه­های نورساختی (فتوسنتزی)، فعالیت آنزیم پراکسیداز، پلی­فنل اکسیداز، میزان پرولین، میزان کربوهیدرات­ها، اندازه­گیری شدند. نتایج نشان داد، که اثر متقابل تنش کادمیوم و کاربرد سیلسیم بر صفات ارتفاع بوته، سطح برگ، وزن خشک ساقه و اندام‌های هوایی و وزن تر ریشه، میزان پرولین، فعالیت آنزیم پراکسیداز و پلی­فنل اکسیداز، میزان سبزینه (کلروفیل)­های a و b  معنی­دار بوده است. بیشترین میزان کربوهیدرات­ها، فعالیت آنزیم پراکسیداز، آنزیم پلی­فنل اکسیداز، میزان سبزینه­های a و b، ارتفاع بوته­ها، وزن خشک ساقه و اندام‌های ­هوایی و سطح برگ­ها در تیمار 1 میلی­مولار سیلسیم و بدون کاربرد کادمیوم به دست آمد. درمجموع نتایج این پژوهش نشان داد، محلول­پاشی گیاه نعناع با سیلسیم در افزایش محتوای سبزینه­های a و b و وزن خشک اندام‌های هوایی، وزن خشک ساقۀ نعناع در شرایط تنش کادمیوم مؤثر بود.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Effect of Silicon nutrition on growth and physiology of spearmint (Mentha spicata L.) under Cadmium stress condition

نویسندگان [English]

  • Mehri Mahdavi 1
  • Behrooz Esmaielpour 2
  • Hamideh Fatemi 3
1 Former M. Sc. Student, Faculty of Agricultural Science , Mohaghegh Ardabili University, Iran
2 Associate Professor, Faculty of Agricultural Science , Mohaghegh Ardabili University, Iran
3 Ph.D. Candidate, Faculty of Agricultural Science, Mohaghegh Ardabili University, Iran
چکیده [English]

Silicon (Si) is the second most abundant element in the earth crust. Silicon has been shown to ameliorate the adverse effects of heavy metals on plants. In order to investigate the effects of silicon nutrition on tolerance of mint (Mentha spicata L.) to cadmium stress, a factorial experiment based on Completely Randomizad Design was conducted in four replications in research greenhouse of Mohaghegh Ardabili University at 2014-2015. Experimental factors included soil contamination by cadmium (0, 50, 100 and 250 mg/kg soil) and silicon nutrition (0 and 1 mM). The number of pots was 32 also in each pot one spearmint stand were planted. During this experiment, traits such as: plant height, plant dry weight, root and stem dry weight, leaf and stem number, leaf area, chlorophylls index, electrolyte leakage, relative water contents, chlorophylls a, chlorophylls b, total chlorophylls,  enzyme activity of peroxidase, polyphenoloxidase, as well as proline, carbohydrates, caretenoids were measured. Results indicated the intractive effects of cd stress and si on plant height, leaf area, plant dry weight, stem dry weight, root wet weight, proline, activity of peroxidase, polyphenoloxidase, chlorophylls a, chlorophylls b, were significant. The highest value for carbohydrate, peroxidase, polyphenoloxidase, chlorophylls a, chlorophylls b, plant height, plant and stem dry weight, leaf area, root wet weight were obtained by foliar spraying of 1 mM concentration of silicon and without cd stress. In general, it can be concluded that foliar spraying of silicon is effective to increase total chlorophylls, plant dry weight, and stem dry weight under cd stress.

کلیدواژه‌ها [English]

  • Heavy metal stress
  • Physiological traits
  • sodium metabisulfite
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