اثر رژیم‌های مختلف آبیاری و اسید سالیسیلیک بر ویژگی های مورفوفیزیولوژیکی گیاه همیشه‌بهار (Calendula officinalis L.) در شرایط اقلیمی زنجان

نوع مقاله: مقاله کامل

نویسندگان

1 دانشجوی سابق کارشناسی ارشد، دانشکدۀ کشاورزی، دانشگاه زنجان

2 استادیار، دانشکدۀ کشاورزی، دانشگاه زنجان

3 دانشیار، دانشکدۀ کشاورزی، دانشگاه زنجان

چکیده

به‌منظور ارزیابی اثر اسید سالیسیلیک بر برخی از ویژگی­های مورفوفیزیولوژیک گیاه همیشه‌بهار در شرایط کم­آبی، آزمایشی به‌صورت کرت­های خرد شده بر پایۀ طرح بلوک­های کامل تصادفی در سه تکرار در شرایط اقلیمی زنجان اجرا شد. تیمار­های آزمایش شامل سه رژیم آبیاری مختلف (33، 66 و 100 درصد نیاز آبی گیاه) به‌عنوان عامل اصلی و اسید سالیسیلیک در چهار سطح (0، 5/0، 1 و 5/1 میلی­مولار) به‌عنوان عامل فرعی بودند. بر پایۀ نتایج به‌دست‌آمده از این پژوهش تنش کم­آبی به‌طور معنی­داری میزان پرولین و فعالیت آنزیم پراکسیداز را افزایش داده و ارتفاع، وزن تر و درصد وزن خشک اندام‌های هوایی، شمار گل، طول عمر گل و محتوای نسبی آب برگ را کاهش داد. کاربرد برگی اسید سالیسیلیک در شرایط تنش کم­آبی در غلظت 1 میلی­مولار به‌طور معنی­داری باعث افزایش وزن تر و درصد وزن خشک اندام هوایی، شمار گل، طول عمر گل، محتوای نسبی آب برگ، فعالیت آنزیم پراکسیداز و میزان پرولین شد. با توجه به نتایج این پژوهش کاربرد برگی اسید سالیسیلک توانست واکنش گیاه در برابر تنش کم­آبی را در بیشتر صفات در سطح احتمال 1 درصد بهبود بخشد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Effect of different irrigation regimes and salicylic acid on morphophysiological characters of Calendula officinalis L. under zanjan climate conditions

نویسندگان [English]

  • Roghayeh Molae 1
  • Mitra Aelaei 2
  • Masood Arghavani 2
  • Jafar Nikbakht 3
1 Former M.Sc. Student, Faculty of Agriculture, University of Zanjan, Zanjan, Iran
2 Assistant Professors, Faculty of Agriculture, University of Zanjan, Zanjan, Iran
3 Associate Professor, Faculty of Agriculture, University of Zanjan, Zanjan, Iran
چکیده [English]

In order to evaluatethe effect of salicylic acid on some morpho physiological charactristics of Calendula officinalis.L. under water deficit stress, an experiment was set out  in split plot design based on RCBD with three replications under Zanjan climate conditions. Treatments consisted of 3 different irrigation regimes(100, 66, 33% ETC) as main plot and SA at 4 levels (0, 0.5, 1, 1.5 mM) as sub plots. Results indicated that, water deficit stress significantly increased proline content and peroxidase enzyme activity and reduced height, fresh and dry weights of shoot, flower number, flower vase life and leaf relative water content (RWC). Foliar application of SA at the 1 mM significantly increased fresh and dry weight of shoot, flower number, flower vase life, relative water content (RWC), peroxidase enzyme activity and proline content under water deficit stress. According to the results of this research, foliar application of salicylic acid could improve plant response in most traits at 1% level to drought stress.

کلیدواژه‌ها [English]

  • Flower number
  • leaf relative water content (RWC)
  • Peroxidase
  • proline
  1. Abdalla, M. M. & El-Khoshiban, N. H. (2007). The influence of water stress on growth, relative water content, photosynthetic pigments, some metabolic and hormonal contents of two Triticumaestivum cultivars. JournalofApplied Sciences Research, 3(12), 2062-2074.
  2. Abedi, T. & Pakniyat, H. (2012). Antioxidantenzyme changes in response to drought stressin ten cultivar of oilsed rap (BrassicanapusL.). Plant Breed, 46(4), 27-34. (in Farsi)
  3. Ali, Q., Ashraf, M. & Athar, H. R. (2007). Exogenously applied proline at different growth stages enhances growth of two maize cultivars grown under water deficit conditions. Pakistan Journal of Botany, 39, 1133-1144.
  4. Amiri dah Ahmadi, S. R., Rezvani Moghaddam, P. & Ahyaii, H. R. (2012). Effect of drought stress on some morphological properties and yield of dill (Anethum graveolens), coriander (Coriandrum sativum) and fennel (Foeniculum vulgare) in greenhouse conditions. Iranian Journal of Field Crops Research, 10(1), 116-124. (in Farsi)
  5. Andalibi, B. & Nouri, F. (2014). Effect of cycocel on photosynthetic activity and essential oil of (Foeniculum vulgare) Mill. under drought stress. Iranian Journal of Plant Biology, 22, 91-104. (in Farsi)
  6. Ashraf, M. (2010). Inducing drought tolerance in plants. Recent advances. Biotechnology Advance, 28, 169-183.
  7. Auge, R. M., Stodola, A. J. W., Moore, J. L., Klingeman, W. E. & Duan, X. (2003). Comparative dehydratation tolerance of foliage of several ornamental crops. Science Horticulture, 98, 511-516.
  8. Bahari, A. A., Sokhtesaraei, R., Chaghazardi, H. R., Masoudi, F. & Nazari, H. (2015). Effect of waterdeficit stress and foliar application of salicylic acidon antioxidants enzymes activity in leaves of Thymusdaenensis subsp.lancifollius. Cercetări Agronomiceîn Moldova, 17(1), 57-67..(in Farsi)                                                
  9. Bates, L. S., Waldran. R. P. & Teare, I. D. (1973). Rapid determination of free proline for water studies. Plant Soil, 39, 205-208.
  10. Belkhadi, A., Hediji, H., Abbes, Z., Nouairi, I., Barhoumi, Z., Zarrouk, M., Chaibi, W. & Djebali, W. (2010). Effects ofexogenous salicylic acid pre-treatment on cadmium toxicity and leaf lipid content in (Linum usitatissimum L.). Ecotoxicology and Environmental Safety, 73(5), 1004-1011.
  11. Bidabadi, S. S., Baninasab, B. & Ghobadi, C. (2012). Influence of salicylicacid on morphological and physiological responses of banana (Musa acuminata cv.‘Berangan’, AAA) shoot tips to in vitrowater stress induced by polyethylene glycol. Plant Omics Journal, 5(1), 33-39. (in Farsi)
  12. Chance, B. & Maehly, A. C. (1955). Assay of catalases and peroxidases. Methods in Enzymology, 2, 764-775.
  13. Demiralay, M., Sağlam, A. & Kadıoğlu, A. (2013). Salicylic acid delays leaf rolling by inducingantioxidantenzymes & modulating osmoprotectant content in Ctenanthe setosa under osmoticstress. Turkish Journal of Biology, 37, 49-59.
  14. Dehghan Niri, F., Saffari, V. R. & Maghsoudi Moud, A. A. (2016). Salicylic acid effects on stomatal characteristics and some growth of calendula plants (Calendula officinalis L.) under salt stress. Journal of Horticultural Science, 47(2), 193-202.
  15. Dole, J. M. & Wilkins, H. F. (2005). Floriculture. Principles and Species.
  16. Ghaderi, N., Talayi, A., Ebadi, A. & Lesani, H. (2010). Effect of drought stress and reirrigation on some physiological properties of grape varieties Sahany, Farokhi and white currants. Horticultural Science, 41(2), 179-188. (in Farsi)
  17. Hassani, A. & Omidbaigi R. (2002). Effect of water stress on some morphological, physiological and metaboliccharacteristics of basil. Agricultural Science, 12(3), 47-59. (in Farsi)
  18. Hashemi Dezfooli, A. & koocheki, A. (1995). Increase crop yield. Jahad Daneshgahi Publications of Mashhad University, P. 360. (in Farsi)
  19. Hayat, Q., Hayat, Sh., Irfan, M. & Ahmad, A. (2010). Effect of exogenous salicylic acid under changing enveironment. A review. Enviromental and Experimental Botany, 68, 14-25. (in Farsi)
  20. Heng Zhou, Z., Guo, K., Abdou Elbaz, A. & Min Yang, Z. (2009). Salicylic acid alleviates mercury Dracocephalum. Chemistry and Bioviversity, 7(8), 1919-1929. 
  21. Hosseini, S. M., Kafi, M. & Arghavan, M. (2016). Effect of salicylic acid on physiological andmorphological characteristics of Lolium perenne cv. Numan under drought stress. Horticulture Sceience, 47(2), (167-176). (in Farsi)
  22. Ichimura, K. & Goto, R. (2002). Effect of Gibberellin A on leaf yellowing and vase life of cut Narcissustazzeta var chinensis flowers. Journal of. Japanes Society of Horticulturae, 69(4), 423-427.
  23. Iqbal, D., Habib, U., Abbasi, N. A. & Chaudhry, A. N. (2012). Improvement in postharvest attributes of Zinnia (Zinnia elegans cv. Benarys Giant) cut flowers by the application of various growth regulators. Pakistan Journal ofBotany, 44, 1091-1094.
  24. Jabbarzadeh, Z., Khosh-Khui, M., & Salehi, H. (2009). The Effect of Foliar-applied Salicylic Acid on Flowering of African Violet. Australian Journal of Basic and Applied Sciences, 3, 4693-4696. (in Farsi)
  25. Jalili Marandi, R., Hassani, A., Abdollahi A. & Hanafi, S. (2011). Improvement of thevase life of cut gladiolus flowers by essentialoils, salicylic acid and silver thiosulfate. Journal of Medicinal Plants Research, 5(20), 5039-5043. (in Farsi)
  26. Jafarzadeh, L., Omidi, H. & Bostani, A. A. (2013). Effect of drought stress and bio-fertilizer on flower yield, photosynthesis pigments and proline content of Marigold (Calendula officinalis L.). Iranian Journal of Medicinal and Aromatic Plants, 29(3), 666-680. (in Farsi)
  27. Kabiri, R., Farahbakhsh, H. & Nasibi, F. (2012). Salicylic acid ameliorates the effectsof oxidative stress induced by water deficit inhydroponic culture of Nigella sativa. Journalof Stress Physiology and Biochemistry, 12(11), 1420-1425. (in Farsi)
  28. Kafi, M., Borzoee, A., Salehi, M. & Kamandi, A. (2009). Physiology of environmental stresses inplants. University of Mashhad. (pp. 58-89). (in Farsi)
  29. Kamali, M., Kharazi, S., Tehranifar, M., & Selahvarzi, Y. (2013). Effect Salicylic asid on growth and Some morpho-physiological traits of Gompherna globosa L. under salt stress. Journal of Horticultural Science, 26(1), 104-112. (in Farsi)
  30. Khalida, K. A. & Teixeira da Silva, J. A. (2010). Yield, essential oil and pigment content of Calendula officinalis L. flower heads cultivated under salt stress conditions. Scientia horticulturae, 126, 297-305.
  31. Ma, Q. Q., Wang, W., Li, Y. H., Li, D. Q. & Zou, Q. (2006). Alleviation of photoinhibition in drought-stressed wheat (Triticum aestivum) by foliar-applied glycinebetaine. Journal of plant physiology, 163(2), 165-175.
  32. Martin, R. J. & Deo, B. (2000). Effect of planpopulation on Calendula flower production. New Zealand. J. Crop and. Journal of Horticultural Science, 28, 37-47.
  33. Masumi, A., Kafi, M., Khazaii, H. R., Davari, K. & Zare Mehrjerdi, M. (2010). Effect of drought stress on water condition and electrolyte leakage, photosynthesis and chlorophyll fluorescence at different growth stages of twonative masses of Kochia Scoparia in salinity conditions. Iranian Journal of Field Crops Research, 10(3), 476-484. (in Farsi)
  34. Mazen, A. (2004). Accumulation of four metals in tissues of Corchorus olitorius and possiblemechanisms of their tolerance. Biologia Plantarum, 48(2), 267-272.
  35. Mei-hua, F., Jian-xin, W., Shi, L. & Fan, G. L. (2008). Salicylic Acid and 6-BA Effects in Shelf-life -Improvement of Gerbera jamesonii Cut Flowers. Anhui Agricultural Science Bulletin.
  36. Metwally, A., Finkemeier, I., Georgi, M., & Dietz, K. J. (2003). Salicylic acid alleviated thecadmium toxicity in barley seedling. Physiology and Biochemistry of Plant, 132, 272-281.
  37. Moradi, M. & Goldani, M. (2010). Evalution of different levels of salicylic acid on some growth indexes of (Calendula officinalis L.) under low irrigation conditions. Environmental Stresses in Crop Sciences, 4(1), 33-45. (in Farsi)
  38. Movahhedi Dehnavi, M., Ranjbar, M., Yadavi, A. R. & Kavusi, B. (2011). Effect ofcycocel on proline, soluble sugares, protein, oil and fatty acids of flax (Linum usitatissimum M.) plants under drought stressin a pot trial Environ. Stresses in Crop Science, 3, 129-138. (in Farsi)
  39. Rahmani, N., Taherkhani, T. & Daneshian, J. (2009). Effect of nitrogen application on physiological yield indexesunder conditions of drought stress in calendula medicinal plants (Calendula officinalis L.). New Findings inAgriculture, 4, 355-365. (in Farsi)
  40. Rampino, P., Spano, G., Pataleo, S., Mita, G., Napier, J. A., Di Fonzo, N., Shewry, P. R. & Perrotta C. (2006). Molecular analysis of a durum wheat stay green mutant: Expression pattern of photosynthesis- related genes. Journal of Cereal Science, 43, 160-168.
  41. Ramrudi, M. & Khamar, A.R. (2013). Interaction effects of foliar spray of salicylic acid and different irrigation treatments on some of the quantity, quality and osmotic regulators of basil. Journal of Plant Ecophysiology AppliedResearch, 1(1), 19-31. (in Farsi)
  42. Raskin, I. (1992). Role of salicylic acid in plants. Ann. Rev. Plant Physiol. Plant Mol. Biol. 43,439-463.
  43. Razmjoo J., Shariatmadari, H., Etemadi, N., Khajealdin, J., Landi, A., Namazi, Y., Borhani, M. &  Aslsni, H. (2004). Effect of environmental stresses on the most important landscaping plants and optimized selected conditions. In: Proceedings of Isfahan municipality landscaping and Parks organization. Research Presenter: Isfahan Industerial University. (in Farsi)
  44. Shakirova, M. F., akhabutdinova, A., Bezrukova, R., Fatkhutdinova, V. R. & Fatkhutdinova, D. R. (2003). Changes in the hormonal status of wheat seedlings induced bysalicylic acid and salinity. Plant Science, 164 (3), 317-322.
  45. Shahrokhi, N. (1996). Quality control methods for raw materials of herbal medicines. 1st. Tehran: Jahad Daneshgahi Publications of Shahid Beheshti University.
  46. Shen, X., Zhou, Y., Duan, L., Li, Z., Enej, A. E. & Li, J. (2010). Silicon effects on photosynthesis andantioxidant parameters of soybean seedlings under drought and ultraviolet-B radiation. Plant
  47. Physiology, 167, 1248-1252.
  48. Shi, Q. & Zhu, Z. (2008). Effects of exogenous salicylic acid on manganesetoxicity, element contentsand antioxidative system in cucumber. Environmental and Experimental Botany 63, 317-326.
  49. Vaziri, Z. H., Salamat, A., Ansari, M., Masihi, M., Heydari, N. & Dehghani sanich, H. (2008). Evapotranspiration plant (water consumption guidelines for plants) (Translation). Publications of the National Committee of Irrigation and Drainage, printing, Tehran. (in Farsi)
  50. Xing, H. L., Tan, L., An, L., Zhao, Z., Wang, S. & Zhang, C. (2004). Evidence for the involvement of nitric oxide and reactive oxygen species in osmotic stress tolerance of wheat seedlings: inverse correlation between leaf abscisicacid accumulation and leaf water loss. Plant Growth Regulation, 42, 61-68.
  51. Yazdanpanah, S., Abasi, F. & Baghzadeh, A. (2010). Effect of salicylic acid and ascorbic acid on proline, sugar and protein content in Satureja hortensis L. under aridity stress. Proceeding of the First National Conference of Environmental Stress in Agricultural Science, 28-29. The University of Birjand. (in Farsi)
  52. Zadehbagheri, M., Al Buali, F., Sadeghi, H. & Javanmardi, Sh. (2014). Evaluation of low irrigation on ionicchanges, RWC, proline content and some apparent characteristics of Petunia. Journal of Horticultural Science, 28(3), 347-359. (in Farsi)
  53. Zargarian, S. M., Tehranifar, A., Nemati, S. H. & Siavashpor, B. (2016). Effect of salicylic acid on morphophysiological traits of Flower bed of Asteraceae under water deficit stress.  Journal of Horticultural Science, 30(1), 151-162. (in Farsi)