تأثیر 24- اپی براسینولید بر برخی ویژگی‌های فیزیولوژی و عملکرد گشنیز (Coriandrum sativum L.) در شرایط تنش خشکی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی سابق کارشناسی ارشد، دانشکده کشاورزی، دانشگاه شهید باهنر کرمان

2 استادیار، دانشکده کشاورزی، دانشگاه شهید باهنر کرمان

چکیده

این آزمایش به‌منظور بررسی تأثیر تنش خشکی و کاربرد 24-اپی براسینولید بر ویژگی‌های فیزیولوژی و پاداکسندگی (آنتی‌اکسیدانی) گیاه دارویی گشنیز، به‌صورت کرت‌های خردشده بر پایۀ بلوک‌های کامل تصادفی با سه تکرار در شهرستان جیرفت اجرا شد. تیمارهای آزمایش شامل سه سطح تنش خشکی به‌صورت آبیاری با تنش ملایم (در پتانسیل ماتریک 2- بار)، آبیاری با تنش متوسط (در پتانسیل ماتریک 5/3- بار) و آبیاری با تنش شدید (در پتانسیل ماتریک 5/4- بار) به‌عنوان عامل اصلی و غلظت‌های 0، 5/0 و 1 میکرومولار 24- اپی براسینولید به‌صورت محلول‌پاشی برگی به‌عنوان عامل فرعی در نظر گرفته شدند. نتایج بررسی‌ها نشان داد، با توجه به معنی‌دار شدن تأثیر سطوح تنش خشکی، بیشترین درصد رطوبت نسبی، میزان سبزینه (کلروفیل)ها، کاروتنوییدها و محتوای پروتئین‌های محلول برگ‌ها در تیمار سطح خشکی ملایم (2- بار) و بیشترین درصد نشت یونی و میزان قندهای احیا در سطح شدید تنش خشکی (5/4- بار) به دست آمد. همچنین تأثیر محلول‌پاشی با 24-اپی براسینولید بر درصد نشت یونی و میزان پروتئین‌های محلول معنی‌دار شد. کمترین درصد نشت یونی و بیشترین میزان پروتئین‌های محلول در گیاهان تحت تیمار 1 میکرومولار براسینولید به‌دست‌آمده است. همچنین اثر متقابل تنش خشکی و محلول‌پاشی نیز بر عملکرد رویشی گشنیز معنی‌دار است. بیشترین میانگین عملکرد رویشی در گیاهان تحت تیمار خشکی خفیف 2- بار و در هر سه غلظت براسینولید به‌دست‌آمده که البته تفاوت معنی‌داری با میانگین به‌دست‌آمده در گیاهان تیمارشده با غلظت 5/0 میکرومولار براسینولید در شرایط تنش سطح متوسط (5/3- بار) نداشت.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Influence of 24-epibrassinolide on some physiological characteristics and the yield of Coriandrum sativum L. under drought stress

نویسندگان [English]

  • Neda Amiri 1
  • Najme Zeinali 2
  • Iraj Tavassolian 2
1 Former M. Sc. Student, Faculty of Agriculture, Shahid Bahonar University of Kerman, Iran
2 Assistant Professor, Faculty of Agriculture, Shahid Bahonar University of Kerman, Iran
چکیده [English]

This experiment was conducted in order to investigate effects of drought stress and 24-epibrassinolide effects on some physiological and antioxidant enzyme activity of medicinal Coriandrum sativum L. as split plot based on randomized complete block design with three replications in Jiroft, south of Kerman. The treatments consisted of three levels of irrigation at -2 bar (moderate drought), irrigation at -3.5 bar(mediate drought) of matric potential, mediate drought and -4.5 bar of matric potential (severe drought) as the main factor and three concentrations of 24-epibrassinolide as foliar spraying (0, 0.5 and 1 µM) as the sub factor. Results showed that according to significant effects of drought levels, the most values of RWC, total chlorophyll, carotenoide content and soluble protein and also, the most values of ion leakage percentage and reduced sugars were obtained in plants treated under moderate drought level (-2 bar). The effect of foliar spraying with 24-epibrassinolide was significant on ion leakage percentage and soluble proteins. The least values of ion leakage percent and the most value of soluble protein were obtaind in plants treated with 1µM of brassinolide. The mutual effects between drought levels and spraying with brassinolide were significant on vegetative yield of coriander. The most average of vegetative yield was obtained in plants under moderate drought at all three concentrations of brassinolide that they had not significant difference with yield average of plants treated by mediate level of drought and 0.5 µM of brassinolide.

کلیدواژه‌ها [English]

  • Carotenoide
  • foliar spraying
  • ion leakage
  • reduced sugar
  • soluble protein

مراجع

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سابقه مقاله

  • تاریخ دریافت: 17 شهریور 1395
  • تاریخ بازنگری: 07 آذر 1395
  • تاریخ پذیرش: 13 آذر 1395

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